Author(s): Baraa Abd Alkareem, Huda I. Al–Qadhi, Fatima S. Salman


DOI: 10.52711/0974-360X.2024.00060   

Address: Baraa Abd Alkareem*, Huda I. Al–Qadhi, Fatima S. Salman
Department of Pharmacology, Collage of Medicine, University of Baghdad/Iraq.
*Corresponding Author

Published In:   Volume - 17,      Issue - 1,     Year - 2024

PCOS is a prevalent endocrine condition affecting women in their reproductive years all over the world. Metabolic abnormalities in women with PCOS frequently include insulin resistance and dyslipidemia, which is characterized by low levels of HDL and increased cholesterol. Metformin, an insulin-sensitizing medication, is commonly used to treat PCOS. Omega-3 fatty acids, found in fish oil supplements, have been shown to have beneficial effects on lipid profiles and reduce inflammation, making them a potential complementary therapy for PCOS. This study aimed to evaluate the effect of a combination of metformin and omega-3 supplements on the lipid profiles of women with PCOS. This study aim to evaluate the effect of using metformin (1000mg/day) and omega 3(1000mg/day) for 3 consecutive months on cholesterol and HDL level (in prospective manner) of patient serum with PCOS. This study was performed at gynecology outpatient clinic and infertile center in AL-batol hospital during the period from December 2021 to march 2022. A total 59 women between (18-40years) with PCOS were randomly assigned into two group, the first group were taking metformin for 12 week and the second group were taking metformin and omega 3 for 12 week. There cholesterol and HDL were measure before and after the treatment .The result show that use of metformin and omega 3 is more significant (P value 0.281) in reducing the level of cholesterol and increase HDL(p value 0.627) than metformin alone (p value 0.525) and (p value 0.106) respectively

Cite this article:
Baraa Abd Alkareem, Huda I. Al–Qadhi, Fatima S. Salman. Metformin and Omega 3 effect on Cholesterol and HDL level in Iraqi women with Poly Cystic Ovary Syndrome. Research Journal of Pharmacy and Technology. 2024; 17(1):385-8. doi: 10.52711/0974-360X.2024.00060

Baraa Abd Alkareem, Huda I. Al–Qadhi, Fatima S. Salman. Metformin and Omega 3 effect on Cholesterol and HDL level in Iraqi women with Poly Cystic Ovary Syndrome. Research Journal of Pharmacy and Technology. 2024; 17(1):385-8. doi: 10.52711/0974-360X.2024.00060   Available on:

1.    Legro RS, Arslanian SA, Ehrmann DA, Hoeger KM, Murad MH, Pasquali R, Welt CK. Diagnosis and treatment of polycystic ovary syndrome: an Endocrine Society clinical practice guideline. The Journal of Clinical Endocrinology and Metabolism. 2013; 98(12): 4565-92.
2.    Norman RJ, Dewailly D, Legro RS, Hickey TE. Polycystic ovary syndrome. The Lancet. 2007 Aug 25;370(9588):685-97.
3.    Al-Hussaniy HA, Alburghaif AH, Naji MA. Leptin hormone and its effectiveness in reproduction, metabolism, immunity, diabetes, hopes and ambitions. Journal of Medicine and Life. 2021; 14(5): 600-606.
4.    Yan F, Zhang J, Zhang L, Zheng XJF, Function. Mulberry anthocyanin extract regulates glucose metabolism by promotion of glycogen synthesis and reduction of gluconeogenesis in human HepG2 cells. 2016;7(1):425-33.
5.    Vazirian M, Nabavi SM, Jafari S, Manayi AJF, Toxicology C. Natural activators of adenosine 5′-monophosphate (AMP)-activated protein kinase (AMPK) and their pharmacological activities. 2018;122:69-79.
6.    Kumar A, Mastana SS, Lindley MRJNrr. n-3 Fatty acids and asthma. 2016;29(1):1-16.
7.    Innes JK, Calder PCJP, Leukotrienes, Acids EF. Omega-6 fatty acids and inflammation. 2018;132:41-8.
8.    Yuan J, Wen X, Jia MJAoPM. Efficacy of omega-3 polyunsaturated fatty acids on hormones, oxidative stress, and inflammatory parameters among polycystic ovary syndrome: a systematic review and meta-analysis. 2021. 2021;10(8):8991-9001.
9.    Al-Qadhi HIJJoPS, Research. Effect of melatonin supplementation on serum LH level and BMI in women with polycystic ovarian syndrome. 2018;10(1):1-4.
10.    Sun X, Vilar S, TatonettiNPJStm. High-throughput methods for combinatorial drug discovery. 2013;5(205):205rv1-rv1.
11.    Ali MA, Rizvi S, Syed BA. Trends in the market for antihypertensive drugs. Nat Rev Drug Discov. 2017;16(5):309-10.
12.    Giles TD, Weber MA, Basile J, Gradman AH, Bharucha DB, Chen W, et al. Efficacy and safety of nebivolol and valsartan as fixed-dose combination in hypertension: a randomised, multicentre study. 2014; 383(9932): 1889-98.
13.    Al-kuraishy AA, Jalil HJ, Mahdi AS, Al-hussaniy HA. General anesthesia in patient with Brain Injury. Medical and Pharmaceutical Journal. 2022; 1(1): 24-34.
14.    Abdulameer AA, Mohammed ZN, Tawfeeq KT. Endoscopic characteristics and management of Subepithelial Lesions in Video-Gastascopie. Medical and Pharmaceutical Journal. 2022; 1(1): 4-13.
15.    Al-Kelaby WJ, Al Kaabi ZS, Alhussaniy HA. Histological and Histochemical Studies of the Eye Structure of Anas Platyrhynchus (Mallard) Duck Species. Indian Vet. J. 2023;100(3):07-15.
16.    Naji MA, Alburghaif AH, Saleh NK, Al-hussaniy HA. Patient expectations regarding consultation with a family doctor: a cross-sectional study. Medical and Pharmaceutical Journal. 2022; 1(1): 35-40.
17.    Ahmed MS, Reyadh AR, Shareef BQ, Ali AR, Hany AH, Meena AN. Increasing Prevalence of Congenital Hypothyroidism in children with Down Syndrome who have a family history of Thyroid disease. Research Journal of Pharmacy and Technology. 2023;16(3):1327-32.
18.    Al-Kuraishy HM, Al-Hussaniy HA, Al-Gareeb AI, Negm WA, El-Kadem AH, Batiha GE, Welson NN, Mostafa-Hedeab G, Qasem AH, Conte-Junior CA. Combination of Panax ginseng CA Mey and Febuxostat Boasted Cardioprotective Effects Against Doxorubicin-Induced Acute Cardiotoxicity in Rats. Frontiers in Pharmacology. 2022;13.
19.    Al-Kuraishy HM, AL‑Gareeb AL, NAJI HA. Febuxostat modulates oxidative and apoptotic pathways in acute doxorubicin‑induced cardiotoxicity: An experimental animal model study. Asian J Pharm Clin Res. 2019;12(4):73-6.
20.    Altalebi RR, Al-hussaniy HA, Al-tameemi ZS, Al-Zobaidy MA, Albu-Rghaif AH, Alkuraishy HM, Hedeab GM, Azam F, Al-Samydai AM, Naji MA. Non-alcoholic fatty liver disease: relation to juvenile obesity, lipid profile, and hepatic enzymes. Journal of Medicine and Life. 2023;16(1):42-7.
21.    Gasmi M, Hooker RW, Maaouia GB, Chikhad AB. The effects of intermittent fasting on markers of health: A narrative review. Medical and Pharmaceutical Journal. 2023;2(2):121-33.
22.    Al-Kuraishy HM, Al-Gareeb AI, Al-Hussaniy HA, Al-Harcan NA, Alexiou A, Batiha GE. Neutrophil Extracellular Traps (NETs) and Covid-19: A new frontiers for therapeutic modality. International Immunopharmacology. 2022:108516.
23.    Al-hussaniy HA, Al-Shammari AH, Sameer HN, Oraibi AI.  The relationship between statin therapy and adipocytokine/inflammatory mediators in dyslipidemic nondiabetic patients: A comparative study. Pharmacia. 2023 Jul 8;70(3):581-5.
24.    Al-Hussaniy HA, Аlmajidi YQ, Oraibi AI, Alkarawi AН. Nanoemulsions as medicinal components in insoluble medicines. Pharmacia. 2023;70(3):537-47.
25.    Sonali S. Gadge, Ashish D. Nakod, Vaishali P. Wasnik, Tilottama M. Gatkine, Shrutika S. Zile, Najuka H. Mohije, Vikrant L. Salode. Development and Validation of Q-absorbance Ratio method for Simultaneous Estimation of Teniligliptin hydrobromide and Metformin HCl in Multicomponent Dosage Form. Asian J. Pharm. Ana. 2019; 9(4):215-218.
26.    Anto Suji, Reeta Jeba Kumari, Nalini Jeyavanth Santha. A Quasi Experimental study to assess the Effectiveness of the Video assisted Teaching Programme on Knowledge and Self Reported Practices Related to concepts of Polycystic Ovary Syndrome among Adolescent girls in Selected Schools, Madurai, Tamil Nadu. Asian J. Nur. Edu. and Research 2016; 6(1): 48-52.
27.    Grace Jebakani Sweety. Assess the effectiveness of cocoa powder in reducing cholesterol level among the hypertensive clients at the rural area, Medavakkam, Chennai. Asian J. Nursing Education and Research. 2020; 10(3): 260-264.
28.    Analytical Method Development and Method Validation for the Simultaneous Estimation of Metformin hydrochloride and Pioglitazone hydrochloride in Tablet Dosage Form by RP-HPLC. Asian J. Pharm. Ana. 2(3): July-Sept. 2012; Page 85-89.
29.    Swapnil D. Jadhav, Akshay A. Magdum, Ramchandra M. Panchal, Pradip S. Koli. Simultaneous Estimation of Metformin Hydrochloride and Rosiglitazone Maleate from Tablet Dosage Form by Derivative Spectroscopic Method. Asian J. Pharm. Ana. 2013; 3(3): 94-97.
30.    Sri Lakshmi D, Jane T Jacob, Srinivasa Sastry D, Satyanarayana D. Simultaneous Estimation of Metformin Glimeperide and Voglibose by RP-UPLC. Asian J. Pharm. Ana. 2017; 7(1): 23-30.
31.    Sushil D. Patil, Sayali K. Chaure, Sanjay Kshirsagar. Development and validation of UV spectrophotometric method for Simultaneous estimation of Empagliflozin and Metformin hydrochloride in bulk drugs. Asian J. Pharm. Ana. 2017; 7(2): 117-123.
32.    AL-Hussaniy HA, AL-Tameemi ZS, AL-Zubaidi BA, Oraibi AI, Naji FA, Kilani S. Pharmacological properties of Spirulina species: Hepatoprotective, antioxidant and anticancer effects. Farmacia. 2023;71(4).
33.    Gandla. Kumara Swamy, R. Lalitha, Ch. Mounika, B. Soumya, D. Sudheer Kumar. A Validated RP-HPLC Method for Simultanious Determination of Metformin and Canagliflozin in Pharmaceutical Formulation. Asian J. Pharm. Ana. 2018; 8(2): 73-77.
34.    Ashok A. Hajare, Vrushali A. Patil . Formulation and Characterization of Metformin Hydrochloride Floating Tablets. Asian J. Pharm. Res. 2022; 2(3):111-117.
35.    Hisham Elrefay, Omnia A. Ismaiel, Wafaa S. Hassan, Abdalla Shalaby . Determination of Glibenclamide and Metformin Hydrochloride in active Pharmaceutical Ingredients and Combined Dosage Form Using a Stability-Indicating HPLC-UV Method. Asian J. Research Chem. 2013; 6(8): 716-721.
36.    Aldin K. The impact of Social Media on Consumers'health Behavior Towards CHOOSING Herbal Cosmetics. Journal of Critical Reviews. 2020;7(9).
37.    Gupta S, Jain R, Kapur S. Statins: A Review of Wonder Drug from Fungus. Research Journal of Pharmacy and Technology. 2014; 7(10): 1201.
38.    Al-hussaniy H, Al-tameemi ZS. Methicillin-Resistant Staphylococcus aureus and New Delhi Metallo beta-lactamases-types of antibiotic resistance, methods of prevention. Medical and Pharmaceutical Journal. 2022; 1(1): 14-24.
39.    Al-hussaniy HA, Altalebi RR, Alburagheef A, Abdul-Amir AG. The Use of PCR for Respiratory Virus Detection on the Diagnosis and Treatment Decision of Respiratory Tract Infections in Iraq. J Pure Appl Microbiol. 2022;16(1):201-6.
40.    Rajitha PB, Naik P. Optimization of growth medium and physicochemical parameters for high-yield biomass production and active metabolites from Aspergillus terreus. Research Journal of Pharmacy and Technology. 2021; 14(6): 2924-30.
41.    Sagheer, O.M., Ahmed, M.S., Jwaid, M.M., Alkhafaje, Z., Al-Hussaniy, H.A., Al-Tameemi, Z.S.The development of molecular docking and molecular dynamics and their application in the field of chemistry and computer simulation.Journal of Medical Pharmaceutical and Allied Sciences. 2023; 12(1): 5552-5562.
42.    Jwaid, M.M. Design, Synthesis, Molecular Docking and Antifungal Evaluation of Mixed Heterocyclic Moieties Containing Pyridine, 1,3,4-Oxadiazole and 1,2,3-Triazol Rings.International Journal of Drug Delivery Technology. 2022; 12(2): 705-710.
43.    Al-Hussaniy HA, Hassan AF, Oraibi AI, Al-Juhaishi AM, Naji FA, Al-Tameemi ZS. Clinical Pharmacogenetics of Angiotensin II Receptor Blockers in Iraq. Journal of Pharmacy and Bioallied Sciences. 2023; 15(3): 101-6.
44.    Sreejith K, Velraj M. Association between Metformin and Cognitive impairment–A Review. Research Journal of Pharmacy and Technology. 2020;13(2):1055-8.

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