Author(s): Agung Krismariono, Carolyn Purnama S

Email(s): agung-k@fkg.unair.ac.id

DOI: 10.52711/0974-360X.2024.00048   

Address: Agung Krismariono1, Carolyn Purnama S2
1Department of Periodontology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
2Student of Periodontic Residency Program, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
*Corresponding Author

Published In:   Volume - 17,      Issue - 1,     Year - 2024


ABSTRACT:
Periodontitis is an irreversible inflammatory disease of the periodontal tissues. The leading cause of periodontitis is Porphyromonas gingivalis bacteria, which secretes virulence factors, namelyLipopolysaccharide (LPS) and proteases, which cause the inflammation of gingival junctional epithelium and loss of attachment, resulting in the formation of a periodontal pocket. The most common type of MMP found in the junctional epithelium is MMP-7. Invasion of Porphyromonas gingivalis bacteria causes an increase in proteolytic activity on the expression and activation of MMP-7, which causes loss of attachment. Efficacious plant extracts, namely irrigated curcumin 1%, have an anti-inflammatory effect. This research is independent research using experimental white rats (Rattus norvegicus) male strain Wistar, 20 weeks old, with a body weight of 325-350 grams. This research is to see the levels of MMP-7 with the ELISA method. Forty-eight periodontitis model rats were divided into two groups. Twenty-four periodontitis model rats as the control group were exposed to Porphyromonas gingivalis, and another twenty-four periodontitis model rats as the treatment group were exposed to Porphyromonas gingivalis and 1% curcumin irrigation. Each group was observed at 24 hours, on days-7 and days-14. The results showed that MMP-7 levels in the treatment group were significantly lower than in the control group on on days-7 and days-14. In the treatment group, MMP-7 levels at 24 hours were significantly lower than on the on days-7 and days-14. In the treatment group, MMP-7 levels did not experience a significant difference between the on days-7 and days-14. The results of this study indicate that 1% curcumin can reduce MMP-7 levels in cases of chronic periodontitis due to Porphyromonas gingivalis bacteria.


Cite this article:
Agung Krismariono, Carolyn Purnama S. MMP-7 Levels Decrease in Gingival Epithelium Exposed to Porphyromonas Gingivalis after Curcumin administration. Research Journal of Pharmacy and Technology. 2024; 17(1):309-3. doi: 10.52711/0974-360X.2024.00048

Cite(Electronic):
Agung Krismariono, Carolyn Purnama S. MMP-7 Levels Decrease in Gingival Epithelium Exposed to Porphyromonas Gingivalis after Curcumin administration. Research Journal of Pharmacy and Technology. 2024; 17(1):309-3. doi: 10.52711/0974-360X.2024.00048   Available on: https://rjptonline.org/AbstractView.aspx?PID=2024-17-1-48


REFERENCES:
1.    Newman, Henry H.Takei, DDS, MS F, Perry R.Klokkeveld, DDS, MS F, Fermin A. Carranza, Dr Odont F. Newman and Carranza’s Clinical Periodontology 13th ed. Newman Carranza’s Clin Periodontol. 2018;47(6):1573–89
2.    Prithi R. Geetha RV. Static Effects of Fruits on Periodontitis. Research J. Pharm. and Tech. 2014; 7(3): 365-367.
3.    Ruchika Agarwal, Lakshmi T.. Salivary Enzymes as Biomarkers for Periodontitis – An Update. Research J. Pharm. and Tech. 2014; 7(1): 98-100.
4.    Puvvalla B, A. S, Mundinamanae DB, M. AS, Bhat D. Unfolding Various Concepts of Junctional Epithelium. J Heal Allied Sci NU. 2018; 8. doi:10.1055/s-0040-1708742
5.    Krismariono A. Less Invasive Vestibule Access Tunneling with Platelet-rich Fibrin Membrane for the Treatment of Gingival Recession. J Contemp Dent Pract. 2019; 20(9): 1024-1028. PMID: 31797823. doi:10.5005/jp-journals-10024-2638
6.    Agung Krismariono, Yunita Purwaningsih. The Influence of 1% Curcumin on Laminin -5γ2 In Periodontitis Condition. Research Journal of Pharmacy and Technology. 2022; 15(7): 3096-0. doi: 10.52711/0974-360X.2022.00518
7.    Ke B, Fan C, Yang L, Fang X. Matrix metalloproteinases-7 and kidney fibrosis. Front Physiol. 2017; 8: 1–7. doi:10.3389/fphys.2017.00021
8.    Vandenbroucke RE, Vanlaere I, Van Hauwermeiren F, Van Wonterghem E, Wilson C, Libert C. Pro-inflammatory effects of matrix metalloproteinase 7 in acute inflammation. Mucosal Immunol. 2014; 7(3): 579-88. doi: 10.1038/mi.2013.76.
9.    Jayawardene KLTD, Palombo EA, Boag PR. Natural Products Are a Promising Source for Anthelmintic Drug Discovery. Biomolecules. 2021; 11(10): 1457. doi: 10.3390/biom11101457.
10.    Tuti Kusumaningsih, Ira Arundina, Tantiana, Rini Devijanti R, Indeswati Diyatri, Mohammed Aljunaid, Poppy Raissa Hudayanti. The Effects of Brotowali (Tinospora crispa) Extract on The Number of Macrophage Cells in Wistar Rats (Rattus norvegicus) Suffering from Periodontitis. Research Journal of Pharmacy and Technology. 2021; 14(6): 3077-1. doi: 10.52711/0974-360X.2021.00538
11.    Prasad S, Tyagi AK, Aggarwal BB. Recent developments in delivery, bioavailability, absorption and metabolism of curcumin: the golden pigment from golden spice. Cancer Res Treat. 2014; 46(1): 2-18. doi: 10.4143/crt.2014.46.1.2
12.    Berbudi A, Rahmi N, Atik N, Wikayani T, Qomarilla N, Rahayu NS, et al. The administration of low-dose Curcuma longa extract induces M2 polarization in peritoneal macrophage culture. Res J Pharm Technol [Internet]. 2021; 14(2): 1079–84. doi:10.5958/0974-360X.2021.00194.3
13.    Kemisha Sanghvi, Chandrasheker K. S, Vasudev Pai, Aswatha Ram H. N. Review on Curcuma longa: Ethnomedicinal uses, Pharmacological Activity and Phytochemical constituents. Research J. Pharm. and Tech. 2020; 13(8): 3983-3986. doi: 10.5958/0974-360X.2020.00704.0
14.    Krismariono, A. (2015). The decreasing of NFκB level in gingival junctional epithelium of rat exposed to Porphyromonas gingivalis with application of 1% curcumin on gingival sulcus. Dental Journal. 2015; 48(1): 35–38. https://doi.org/10.20473/j.djmkg.v48.i1.p35-38
15.    Orozco CM, Belnap DM. Control of Matrix Metalloproteinases in a Periodontitis Model : Molecules That Trigger or Inhibit MMP Production. 2016; https://scholarsarchive.byu.edu/etd/6242
16.    Xie YL, Chu JG, Jian XM, Dong JZ, Wang LP, Li GX, et al. Curcumin attenuates lipopolysaccharide/D-galactosamine-induced acute liver injury by activating Nrf2 nuclear translocation and inhibiting NF-kB activation. Biomed Pharmacother. 2017; 91: 70-77.  doi: 10.1016/j.biopha.2017.04.070
17.    Mysak J, Podzimek S, Sommerova P, Lyuya-Mi Y, Bartova J, Janatova T, Prochazkova J, Duskova J. Porphyromonas gingivalis: major periodontopathic pathogen overview. J Immunol Res. 2014; 2014: 476068. doi: 10.1155/2014/476068
18.    Elgezawi M, Haridy R, Almas K, Abdalla MA, Omar O, Abuohashish H, Elembaby A, Christine Wölfle U, Siddiqui Y, Kaisarly D. Matrix Metalloproteinases in Dental and Periodontal Tissues and Their Current Inhibitors: Developmental, Degradational and Pathological Aspects. Int J Mol Sci. 2022; 23(16):1-17. doi: 10.3390/ijms23168929.
19.    Kivelä-Rajamäki M, Maisi P, Srinivas R, Tervahartiala T, Teronen O, Husa V, Salo T, Sorsa T. Levels and molecular forms of MMP-7 (matrilysin-1) and MMP-8 (collagenase-2) in diseased human peri-implant sulcular fluid. J Periodontal Res. 2003; 38(6): 583-90. doi: 10.1034/j.1600-0765.2003.00688.x.
20.    Cury PR, Canavez F, de Araújo VC, Furuse C, de Araújo NS. Substance P regulates the expression of matrix metalloproteinases and tissue inhibitors of metalloproteinase in cultured human gingival fibroblasts. J Periodontal Res. 2008; 43(3): 255-60. doi: 10.1111/j.1600-0765.2007.01022.x.
21.    Prafulla S. Lata P. Priya R.Vidya S. Novel Curcumin Derivatives: Targeted for Anti-Inflammatory Activity. Asian J. Research Chem. 2019; 12(2): 49-54. doi: 10.5958/0974-4150.2019.00011.7
22.    Nair TS, Meghana R, Shlini P. Antimicrobial Activity of the protein fraction obtained in the extraction of Curcumin. Asian J Res Chem. 2019; 12(4): 199-202. doi: 10.5958/0974-4150.2019.00037.3
23.    Xiao CJ, Yu XJ, Xie JL, Liu S, Li S. Protective effect and related mechanisms of curcumin in rat experimental periodontitis. Head Face Med. 2018; 14(1): 12. doi: 10.1186/s13005-018-0169-1
24.    V. V, Jyotsna NL, Bakshi V, Ismail S, Mohanty D, T. K, et al. Optimization of Extraction of Curcuminoids from Turmeric Powder (Curcuma longa). Research J. Pharm. and Tech. 2021; 14(9): 4615-4620.doi: 10.52711/0974-360X.2021.00802
25.    Sri Vasavi Reddy A, J. Suresh, Hemant K.S. Yadav, Apurva Singh. A Review on Curcuma longa. Research J. Pharm. and Tech. 2012; 5(2):158-165.
26.    Saha S, Roy A, Bahadur S, Choudhury A. Bioenhamcement of Curcumin by Dual approach. Res J Pharm Technol. 2016; 9(8): 1059-1063. doi :10.5958/0974-360X.2016.00200.6
27.    Kishu Tripathi. Curcumin-The Spice of Life-I. Res J Pharmacogn Phytochem. 2009; 1(3): 153-161
28.    Suhag A, Dixit J, Dhan P, Prakash D. Role of curcumin as a subgingival irrigant: a pilot study. Perio. 2007; 4(2): 115–21.
29.    Livada R, Shiloah J, Tipton DA, Dabbous MK. The Potential Role of Curcumin in Periodontal Therapy: A Review of the Literature. J Int Acad Periodontol. 2017; 19(3): 70-79.
30.    Gharib SA, Altemeier WA, Van Winkle LS, Plopper CG, Schlesinger SY, Buell CA, Brauer R, Lee V, Parks WC, Chen P. Matrix metalloproteinase-7 coordinates airway epithelial injury response and differentiation of ciliated cells. Am J Respir Cell Mol Biol. 2013; 48(3): 390-6. doi: 10.1165/rcmb.2012-0083OC.
31.    Lundmark A, Johannsen G, Eriksson K, Kats A, Jansson L, Tervahartiala T, Rathnayake N, Åkerman S, Klinge B, Sorsa T, Yucel-Lindberg T. Mucin 4 and matrix metalloproteinase 7 as novel salivary biomarkers for periodontitis. J Clin Periodontol. 2017; 44(3): 247-254. doi: 10.1111/jcpe.12670
32.    Augustina, E. F., Sudiana, I. K., Soetjipto, & Rubianto, M. Expression of NF-κB and MMP-7 on defenses of the gingival epithelium injected lps porphyromonas gingivalis with the administration of curcumin. Journal of International Dental and Medical Research. 2019; 12(3): 941-946.
33.    Sunagawa Y, Funamoto M, Sono S, Shimizu K, Shimizu S, Genpei M, Miyazaki Y, Katanasaka Y, Morimoto E, Ueno M, Komiyama M, Kakeya H, Wada H, Hasegawa K, Morimoto T. Curcumin and its demethoxy derivatives possess p300 HAT inhibitory activity and suppress hypertrophic responses in cardiomyocytes. J Pharmacol Sci. 2018; 136(4): 212-217. doi: 10.1016/j.jphs.2017.12.013
34.    Okahashi N, Inaba H, Nakagawa I, Yamamura T, Kuboniwa M, Nakayama K, Hamada S, Amano A. Porphyromonas gingivalis induces receptor activator of NF-kappaB ligand expression in osteoblasts through the activator protein 1 pathway. Infect Immun. 2004; 72(3): 1706-14. doi: 10.1128/IAI.72.3.1706-1714.2004

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

1.3
2021CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags


Not Available