Author(s): Tesfay Weletnsae, Tekleweyni Tadege, Kidu Hintsa, Zenebe Hagos, Davuluri Syam Babu, John Dogulas Palleti, Godavarthi Rajan Bhagyasri, Sudhish Rai, Krishna Chaithanya K

Email(s): krishanchaitanyawc@gmail.com

DOI: 10.52711/0974-360X.2024.00038   

Address: Tesfay Weletnsae1, Tekleweyni Tadege1, Kidu Hintsa1, Zenebe Hagos1, Davuluri Syam Babu2, John Dogulas Palleti3, Godavarthi Rajan Bhagyasri4, Sudhish Rai5, Krishna Chaithanya K1*,
1Department of Chemistry, College of Natural and Computational Sciences, Aksum University, Axum, Ethiopia.
2Department of Biotechnology, Vignan's Foundation for Science, Technology and Research, Vadlamudi, 522213 Guntur, Andhra Pradesh, India.
3Research and Development, Centre for Computational and Biological Sciences, 48 -12-17, Srinagar, Near RTC Complex, Visakhapatnam - 530016, Andhra Pradesh. India.
4Department of Chemistry, Jawaharlal Nehru Technological University, Anantapur - 515002, A. P. India.
5Department of Pharmacognosy, Tagore Institute of Pharmacy and Research, Bilaspur, Chhattisgarh, India.
*Corresponding Author

Published In:   Volume - 17,      Issue - 1,     Year - 2024


ABSTRACT:
Background: The clinical management of the inflammatory diseases by using NSAIDs and selective COX-2 inhibitors, shunting arachidonic acid pathway to the 5-LOX pathway, which is associated with the production of high amount of Leukotrienes and cysteinyl-leukotrienes, which are involved in pathophysiological of various type of cancers and inflammatory diseases. The substantial efforts are being made to the development of dual inhibitors against Cyclooxygenase (COX-2) and 5- Lipoxygenase (5-LOX) should be more growing demand for efficient as anti-inflammatory medicine with lower side effects than the currently available drugs. Maytenus arbutifolia (M. arbutifolia) is medicinal plant belongs to the family Celastraceae, and the leaves of M.arbutifolia have been used for the treatment of skin infections and inflammatory related disease. Objective: The current study aimed to examine the in vitro screening of M. arbutifolia leaf organic extracts for the development of anti-inflammatory agents through evaluate the inhibitory effect on Cyclooxygenase- 1and2 and 5- Lipoxygenases enzymes. Methods: The powdered dried leaves of the M. arbutifolia (100 g) was extracted with successive Soxhlet extraction by using solvents of low polarity (Petroleum ether) to a high polar solvent (Ethanol). These leaf extracts were evaluated for the in vitro anti-inflammatory activity by 5- Lipooxygenase, Cyclooxygenase-1 and Cyclooxygenase-2 inhibitory activities by ELISA method. Results: Among the tested extracts, the ethanolic leaf extracts of M.arbutifolia showed (MALET) potential 5-LOX inhibition i.e.77.58 % at 100µg/ml with IC50 of 23.76µg/ml, and also exhibited two fold COX-2 versus COX-1 inhibition, with potent COX-2 inhibitory effect with IC50 of 30.35µg/ml. The promising anti-inflammatory activity exhibited by MALET was due to the presence of secondary metabolites such as flavonoids, tannins and polyphenolic compounds. Conclusion: The current study concluded that ethanolic leaf extracts of M.arbutifolia is a promising source for isolation of 5-LOX and COX-2 dual inhibitory compound and highlights the potential traditional remedies for managing of inflammatory related diseases and cancers.


Cite this article:
Tesfay Weletnsae, Tekleweyni Tadege, Kidu Hintsa, Zenebe Hagos, Davuluri Syam Babu, John Dogulas Palleti, Godavarthi Rajan Bhagyasri, Sudhish Rai, Krishna Chaithanya K. In vitro Screening of 5-LOX /COX-2 Dual Inhibitors from selected Medicinal plant Maytenus arbutifolia. Research Journal of Pharmacy and Technology. 2024; 17(1):241-8. doi: 10.52711/0974-360X.2024.00038

Cite(Electronic):
Tesfay Weletnsae, Tekleweyni Tadege, Kidu Hintsa, Zenebe Hagos, Davuluri Syam Babu, John Dogulas Palleti, Godavarthi Rajan Bhagyasri, Sudhish Rai, Krishna Chaithanya K. In vitro Screening of 5-LOX /COX-2 Dual Inhibitors from selected Medicinal plant Maytenus arbutifolia. Research Journal of Pharmacy and Technology. 2024; 17(1):241-8. doi: 10.52711/0974-360X.2024.00038   Available on: https://rjptonline.org/AbstractView.aspx?PID=2024-17-1-38


REFERENCES:
1.    Linlin C, Huidan D, Hengmin C, Jing F, Zhicai Z, et.al. Inflammatory responses and inflammation-associated diseases in organs. Oncotarget. 2018; 9(6): 7204-7218. https://doi.org/10.18632/oncotarget.23208.
2.    Jiri G, Milan R, Eva K, Stuart BG. Inflammation and its resolution and the musculoskeletal system. Journal of Orthopedic Translation. 2017; 10:52-67. https://doi.org/10.1016/j.jot.2017.05.007.
3.    Uttara B, Singh AV, Zamboni P, Mahajan RT. Oxidative stress and neurodegenerative diseases: a review of upstream and downstream antioxidant therapeutic options. Current Neuropharmacology, 2009; 7(1):65-74. https://doi.org/10.2174/157015909787602823.
4.    Kany S, Vollrath JT, Relja B. Cytokines in Inflammatory Disease. International Journal of Molecular Sciences. 2019; 20(23): 6008. https://doi.org/10.3390/ijms20236008.
5.    Pratheesh kumar P, Sreekala C, Zhang Z, Budhraja A, Ding S. et.al Cancer prevention with promising natural products: mechanisms of action and molecular targets. Anticancer Agents in Medicinal Chemistry. 2012; 12(10): 1159-84. https://doi.org/10.2174/187152012803833035.
6.    Yona S, Gordon S. Inflammation: Glucocorticoids turn the monocyte switch. Immunology and Cell Biology. 2007; 85(2):81-2. https://doi.org/10.1038/sj.icb.7100034.
7.    Ramamoorthy S, Cidlowski JA. Corticosteroids: Mechanisms of Action in Health and Disease. Rheumatic Disease Clinics of North America. 2016; 42(1):15-31. https://doi.org/10.1016/j.rdc.2015.08.002.
8.    Vane JR,  Botting RM. Anti-inflammatory drugs and their mechanism of action. Inflammation Research. 1998; 47(2): S78-87.  https://doi.org/10.1007/s000110050284.
9.    Kleiman A, Tuckermann JP. Glucocorticoid receptor action in beneficial and side effects of steroid therapy: lessons from conditional knockout mice.  Molecular and Cellular Endocrinology. 2007; 15; 275(1-2): 98-108. https://doi.org/10.1016/j.mce.2007.05.009.
10.    Goldstein JL, Cryer B. Gastrointestinal injury associated with NSAID use: a case study and review of risk factors and preventative strategies. Drug, Healthcare and Patient Safety. 2015; 22; 7:31-41. https://doi.org/10.2147/dhps.s71976.
11.    Ziboh VA, Naguwa S, Vang K, Wineinger J, Morrissey BM et al. Suppression of leukotriene B4 generation by ex-vivo neutrophils isolated from asthma patients on dietary supplementation with gammalinolenic acid-containing borage oil: possible implication in asthma. Clinical and Developmental Immunology. 2004; 11(1): 13-21. https://doi.org/10.1080/10446670410001670445.
12.    Skelly MM, Hawkey CJ. Dual COX inhibition and upper gastrointestinal damage.  Current Pharmaceutical Design.2003; 9(27):2191-5.https://doi.org/10.2174/1381612033453965.
13.    Elaine E, Tania AA, Ruti RR, Domingos SN, Anado CTC. Analagesic activity of Psychrotriacolorata (Willd. ex R. and S.) Muell. Arg. alkaloids.  Journal of Ethno Pharmacology.  1995: 48(2): 77-83.https://doi.org/10.1016/0378-8741(95)01287-n.
14.    GakunjuD, Mberu E, DossajiS, Gray A, Waigh R, Waterman P, Watkins W. Potent antimalarial activity of the alkaloid nitidine, isolated from a Kenyan herbal remedy. Antimicrobial Agents and Chemotherapy. 1995; 39(12): 2606-2609. https://doi.org/10.1128/aac.39.12.2606.
15.    Das S, Haldar PK, Pramanik G, Suresh Kumar RB. Evaluation of anti-inflammatory activity of Clerodendron infortunatum Linn. extract in rats. Global Journal of Pharmacology. 2010; 4(1): 48-50. https://doi.org/10.1007/s13300-010-0019-z.
16.    Bisht R, Bhattacharya S, Jaliwala YA. COX and LOX inhibitory potential of Abroma augusta and Desmodium gangeticum. The Journal of Phytopharmacology. 2014; 3: 168-175. https://doi.org/10.31254/phyto.2014.3303.
17.    Kumar RS, Rajkapoor B, Perumal P, Kumar SV, Geetha AS.Beneficial effects ofmethanolic  extract of Indigofera linnaei Ali. on the inflammatory and nociceptive responses in rodent models. Brazilian Journal of Pharmaceutical Sciences. 2016; 52(1): 113-123. https://doi.org/10.1590/s1984-82502016000100013.
18.    Audu SA, Mohammed I, Kaita HA. Phytochemical screening of the leaves of Lophira lanceolata (Ochanaceae). Life Science Journal. 2007; 4(4): 75-79.
19.    Ayoola GA, Coker HAB, Adesegun SA, Adepoju-Bello AA, Obaweya K et.al. Phytochemical screening and antioxidant activities of some selected medicinal plants used for malaria therapy in south western Nigeria. Tropical Journal of Pharmacy Research. 2008; 7(3): 1019-1024. https://doi.org/10.4314/ijhr.v1i2.47920.
20.    Yarla NS, Bishayee A, Sethi G, Reddanna P, Kalle AM, Dhananjaya BL, Duddukuri, GR. Targeting arachidonic acid pathway by natural products for cancer prevention and therapy. Seminars in Cancer Biology. 2016; 40-41: 48-8. https://doi.org/10.1016/j.semcancer.2016.02.001.
21.    Reddy DB, Reddy TC, Jyotsna G, Sharan S, Priya N, Lakshmipathi V, Reddanna P. Chebulagic acid, a COX-LOX dual inhibitor isolated from the fruits of Terminalia chebula Retz induces apoptosis in COLO-205 cell line. Journal of Ethnopharmacology. 2009; 124(3): 506-12. https://doi.org/10.1016/j.jep.2009.05.022.
22.    Shang JH, Cai XH, Feng T, Zhao YL, Wang JK, Zhang LY, Yan M, Luo XD. Pharmacological evaluation of Alstonia scholaris: anti-inflammatory and analgesic effects. Journal of Ethnopharmacology. 2010; 129(2): 174-81. https://doi.org/10.1016/j.jep.2010.02.011.
23.    Khan H, Ali Khan M, Hussan I.  Enzyme inhibition activities of the extracts from rhizomes of Gloriosa superba Linn (Colchicaceae). Journal of enzyme inhibition and Medicinal Chemistry 2007; 22(6), 722-725. https://doi.org/10.1080/14756360601164853.

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