Author(s): Shivangi Saxena, Dipjyoti Chakraborty


DOI: 10.52711/0974-360X.2024.00033   

Address: Shivangi Saxena, Dipjyoti Chakraborty*
Department of Bioscience and Biotechnology, Banasthali Vidyapith, Rajasthan - 304022, India.
*Corresponding Author

Published In:   Volume - 17,      Issue - 1,     Year - 2024

Bauhinia variegata L. is reported to have medicinal properties in traditional literature. The antioxidant activities of the ethanolic and aqueous extracts were evaluated by the DPPH method, phoshomolybdenum assay, reducing power method. Characterization of leaf ethanolic extract was done by GC-MS. The major compounds identified by GC-MS are cyclopropanebutanoic acid, 2-[[2-[[2-[(2-pentylcyclopropyl) methyl] cyclopropyl]methyl] cyclopropyl]methyl] and minor compounds are, 2,7-Diphenyl-1,6-dioxopyridazino[4,5 :2',3']pyrrolo[4',5'-d]pyridazine, Ethyl iso-allocholate, Dasycarpidan-1-methanol, acetate. The compounds identified in this study have pharmacological value, anti-microbial properties and show anti-oxidant activity.

Cite this article:
Shivangi Saxena, Dipjyoti Chakraborty. Antioxidant activity and GC-MS Analysis of Bauhinia variegata L. (Fabaceae). Research Journal of Pharmacy and Technology. 2024; 17(1):208-2. doi: 10.52711/0974-360X.2024.00033

Shivangi Saxena, Dipjyoti Chakraborty. Antioxidant activity and GC-MS Analysis of Bauhinia variegata L. (Fabaceae). Research Journal of Pharmacy and Technology. 2024; 17(1):208-2. doi: 10.52711/0974-360X.2024.00033   Available on:

1.    Okigbo RN. Eme UE. Ogbogu S. Biodiversity and conservation of medicinal and aromatic plants in Africa. Biotechnology and Molecular Biology Reviews. 2008; 3(6): 127-134.
2.    Promprom W. Chatan W. GC-MS Analysis and antioxidant activity of Bauhinia nakhonphanomensis leaf ethanolic extract. Pharmacognosy Journal. 2017; 9(5):663-667. doi.10.5530/pj.2017.5.105
3.    Owolabi OJ. Omogbai EK.Obasuyi O. Antifungal and antibacterial activities of the ethanolic and aqueous extract of Kigelia africana (Bignoniaceae) stem bark. African Journal of Biotechnology. 2007; 6(14): 1677-1680.
4.    Malairajan P. Gopalakrishnan G.Narasimhan S.Veni KJ. Analgesic activity of some Indian medicinal plants. Journal of Ethnopharmacology. 2006; 106(3): 425-428. doi.
5.    Sengupta R. Desai D. Physicochemical and preliminary phytochemical studies on Kachnar bark (Bauhinia variegata). Der Pharmacia Lettre. 2015; 7(7): 67-71.
6.    Chakraborty D. Mandal SM. Chakraborty J. Bhattacharyaa PK. Bandyopadhyay A. Mitra A. Gupta K. Antimicrobial activity of leaf extract of Basilicumpolystachyon (L) Moench. 2007; 45(8): 744-748. doi.
7.    Chakraborty D.Mandal SM. Fractional changes in phenolic acids composition in root nodules of Arachis hypogaea L. Plant Growth Regulation. 2008; 55: 159-163.doi.
8.    Singleton VL. Orthofer R.Lamuela-Raventós RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. In: Methods in Enzymology. 1999; 299: 152-178. doi.
9.    Bais HP. Walker TS. Stermitz FR. Hufbauer RA.Vivanco JM. Enantiomeric-dependent phytotoxic and antimicrobial activity of catechin Arhizo secreted racemic mixture from Spotted Knapweed. Plant Physiology. 2002; 128 (4):1173-1179. doi.
10.    Alam MN. Bristi NJ. Rafiquzzaman M. Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharmaceutical Journal. 2013; 21(2): 143-152. doi.
11.    Ruch RJ. Cheng SJ. Klaunig JE. Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis. 1989; 10(6): 1003-1008. doi.
12.    Al-Gara NI. Abu-Serag NA. Shaheed KA. Al Bahadly ZK. Analysis of bioactive phytochemical compound of (Cyperus alternifolius L.) by using gas chromatography–mass spectrometry. In: IOP conference series: Materials Science and Engineering. 2019; 571(1): 012047. doi.10.1088/1757-899X/571/1/012047
13.    Alam R. Imon RR. Talukder ME. Akhter S. Hossain MA. Ahammad F. Rahman MM. GC-MS analysis of phytoconstituents from Ruellia prostrata and Senna tora and identification of potential anti-viral activity against SARS-CoV-2. RSC Advances. 2021; 11(63): 40120-40135. doi.10.1039/D1RA06842C
14.    Khan N. Ali A. Qadir A. Ali A. Warsi MH. Tahir A. Ali A. GC-MS analysis and antioxidant activity of Wrightia tinctoria R. Br. leaf extract. Journal of AOAC International. 2021; 104(5): 1415-1419. doi.
15.    Malathi K. Ramaiah S. Ethyl iso-allocholate from medicinal rice Karungkavuni inhibits dihydropteroate synthase in Escherichia coli: A molecular docking and dynamics study. Indian Journal of Pharmaceutical Sciences. 2017; 78(6):780-788. doi.10.4172/pharmaceutical-sciences.1000184
16.    Al MJ. Mohammed GJ. Anti-bacterial, antifungal activity and chemical analysis of Punica grantanum (Pomegranate peel) using GC-MS and FTIR Spectroscopy. International Journal of Pharmacognosy and Phytochemical Research. 2016; 8(3): 480-494
17.    Kamble VA. Moon AH. Bioassay-guided fractional isolation and identification of anti-MRSA compounds by GC-MS from Tridax procumbens L. World Journal of Pharmacy and Pharmaceutical Sciences. 2015; 4(6): 1022-1031. url:
18.    Grassmann J. Hippeli S. Elstner EF. Plant’s defence and its benefits for animals and medicine: role of phenolics and terpenoids in avoiding oxygen stress. Plant Physiology and Biochemistry. 2002; 40(6-8): 471-478. doi.
19.    Mandal SM. Chakraborty D. Dey S. Phenolic acids act as signaling molecules in plant-microbe symbioses. Plant Signaling and Behavior. 2010; 5(4): 359-368. doi.
20.    Jin T. Liu L. Zhang H. Hicks K. Antimicrobial activity of nisin incorporated in pectin and polylactic acid composite films against Listeria monocytogenes. International Journal of Food Science and Technology. 2009; 44(2): 322-329. doi.
21.    Bairagi SM. Agrawal RC. Nema N. Antimicrobial activity of Bauhinia variegata leaves (ethanolic) extract. Research Journal of Pharmacognosy and Phytochemistry. 2011; 3(5): 244-246.
22.    Gunalan G. Saraswathy A.Vijayalakshmi K. Preliminary phytochemical and pharmacognostical analysis of Bauhinia variegata Linn. leaves. Research Journal ofPharmacognosy and Phytochemistry. 2011; 3(5): 236-240.
23.    Khare P. Kishore K. Sharma DK. Catalase and superoxide dismutase (SOD) activity in Swiss Albino mice treated with ethanolic leaf extract of Bauhinia variegata. Research Journal of Pharmacy and Technology. 2019; 12(9): 4259-4262. doi.10.5958/0974-360X.2019.00764.9
24.    Pethani T. Thumar M. Vaishnav D. Dudhrejiya D. A concise study of pharmacognostic and antioxidant potential of stem bark from Bauhinia variegata Linn. Research Journal of Pharmacy and Technology. 2018; 11(8): 3250-3256. doi.10.5958/0974-360X.2018.00597.8
25.    Sharma RK. Rajni GP. Nathiya D. Sharma AK. Assessment of wound healing activity of roots of Bauhinia variegata Linn. by excision and incision model in Albino Rats. Asian Journal of Research in Pharmaceutical Sciences. 2015; 5(3): 145-152. doi.10.5958/2231-5659.2015.00023.5
26.    Jadhav AG. Narkhede SB. Patil MB. Vidyasagar G. Hepatoprotective Activity of Bauhinia variegata bark against carbon tetrachloride induced hepatotoxicity in female Albino Wistar rats. Research Journal of Pharmacology and Pharmacodynamics. 2010; 2(3): 239-242.
27.    Deep P. Mishra A. Verma N. Pharmacognostical evaluation and HPTLC analysis of two medicinally important secondary metabolites in Bauhinia variegata leaves from Gorakhpur district in summer season. Research Journal of Pharmacy and Technology. 2020; 13(6): 2667-2671. doi.10.5958/0974-360X.2020.00474.6  
28.    Mohammed M. Mahdi MF. Talib B. Ibrahim SA. Identification and Isolation of lupeol and β-sitosterol from Iraqi Bauhinia variegata and determination the cytotoxic activity of the hexane extract of its leaves, stems and flowers. Research Journal of Pharmacy and Technology. 2021; 14(11): 5703-8. doi.10.52711/0974-360X.2021.00991
29.    Jain T. Dubey D. Jain V. Dashora K. Regulatory status of traditional medicines in different countries: an overview. Research Journal of Pharmacy and Technology. 2011;4(7): 1007-1015.
30.    Gulalkari RA. Preparation, characterization and pharmacological evaluation of Bauhinia variegata Lauha Bhasma. Research Journal of Pharmacy and Technology. 2022; 15(11): 5295-5301. doi.10.52711/0974-360X.2022.00892
31.    Shukla SS. Sharma V. Gidwani B. Vyas A. Daharwal SJ. Pandey RK. Chromatographic fingerprint: amodern scientific tool for standardization of traditional medicines. Research Journal of Pharmacy and Technology. 2021; 14(7): 4003-4010. doi.10.52711/0974-360X.2021.00694

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

56th percentile
Powered by  Scopus

SCImago Journal & Country Rank

Recent Articles


Not Available