Author(s): Shaimaa Elsayed, Alaa El-Gindy, Yasser El-Shabrawy, ElSayed A. Ibrahim

Email(s): shaimaaharira@yahoo.com , alaa.elgindy@hotmail.com

DOI: 10.52711/0974-360X.2024.00012   

Address: Shaimaa Elsayed1,2, Alaa El-Gindy2, Yasser El-Shabrawy1,3 , ElSayed A. Ibrahim2
1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Horus University - Egypt, New Damietta, Egypt.
2Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt.
3Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt.
*Corresponding Author

Published In:   Volume - 17,      Issue - 1,     Year - 2024


ABSTRACT:
This paper elucidates the process of developing and validating the UPLC technique, aiming to enable the concurrent measurement of tenofovir and amoxicillin. This UPLC method is accurate and sensitive. The experimental setup involved utilizing a Kinetex 1.7µ C18 100A (2.1-mm × 50-mm) column maintained at a temperature of 30°C. The mobile phase employed a combination of methanol and phosphate buffer (0.1% orthophosphoric acid in water, pH 3.5) in a ratio of 30:70 (v/v). The flow rate was set at 0.2mL/min, and the detection wavelength for analysis was 230nm. All these conditions resulted in optimum chromatographic separation. This developed method of UPLC took less than 4 minutes to separate this mixture. The detection limits for two medications were determined to be 0.45µg/mL for tenofovir and 0.26µg/mL for amoxicillin. The calibration plots of both substances exhibited satisfactory linearity within the concentration range of 1.0 - 2.0 µg/mL. Remarkably, the method demonstrated excellent performance with high percentage recoveries ranging from 96.8% to 102.92% and low percentage relative standard deviation (%RSD) values below 2%. Consequently, the proposed methodology proved to be highly effective for detecting the targeted drugs in their respective dosage forms.


Cite this article:
Shaimaa Elsayed, Alaa El-Gindy, Yasser El-Shabrawy, ElSayed A. Ibrahim. A Green UPLC Method for the Simultaneous determination of Tenofovir and Amoxicillin in Biological Fluids and Dosage Forms. Research Journal of Pharmacy and Technology. 2024; 17(1):74-0. doi: 10.52711/0974-360X.2024.00012

Cite(Electronic):
Shaimaa Elsayed, Alaa El-Gindy, Yasser El-Shabrawy, ElSayed A. Ibrahim. A Green UPLC Method for the Simultaneous determination of Tenofovir and Amoxicillin in Biological Fluids and Dosage Forms. Research Journal of Pharmacy and Technology. 2024; 17(1):74-0. doi: 10.52711/0974-360X.2024.00012   Available on: https://rjptonline.org/AbstractView.aspx?PID=2024-17-1-12


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