Author(s): Shaimaa Elsayed, Alaa El-Gindy, Yasser El-Shabrawy, ElSayed A. Ibrahim

Email(s): shaimaaharira@yahoo.com , alaa.elgindy@hotmail.com

DOI: 10.52711/0974-360X.2024.00012   

Address: Shaimaa Elsayed1,2, Alaa El-Gindy2, Yasser El-Shabrawy1,3 , ElSayed A. Ibrahim2
1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Horus University - Egypt, New Damietta, Egypt.
2Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt.
3Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt.
*Corresponding Author

Published In:   Volume - 17,      Issue - 1,     Year - 2024


ABSTRACT:
This paper elucidates the process of developing and validating the UPLC technique, aiming to enable the concurrent measurement of tenofovir and amoxicillin. This UPLC method is accurate and sensitive. The experimental setup involved utilizing a Kinetex 1.7µ C18 100A (2.1-mm × 50-mm) column maintained at a temperature of 30°C. The mobile phase employed a combination of methanol and phosphate buffer (0.1% orthophosphoric acid in water, pH 3.5) in a ratio of 30:70 (v/v). The flow rate was set at 0.2mL/min, and the detection wavelength for analysis was 230nm. All these conditions resulted in optimum chromatographic separation. This developed method of UPLC took less than 4 minutes to separate this mixture. The detection limits for two medications were determined to be 0.45µg/mL for tenofovir and 0.26µg/mL for amoxicillin. The calibration plots of both substances exhibited satisfactory linearity within the concentration range of 1.0 - 2.0 µg/mL. Remarkably, the method demonstrated excellent performance with high percentage recoveries ranging from 96.8% to 102.92% and low percentage relative standard deviation (%RSD) values below 2%. Consequently, the proposed methodology proved to be highly effective for detecting the targeted drugs in their respective dosage forms.


Cite this article:
Shaimaa Elsayed, Alaa El-Gindy, Yasser El-Shabrawy, ElSayed A. Ibrahim. A Green UPLC Method for the Simultaneous determination of Tenofovir and Amoxicillin in Biological Fluids and Dosage Forms. Research Journal of Pharmacy and Technology. 2024; 17(1):74-0. doi: 10.52711/0974-360X.2024.00012

Cite(Electronic):
Shaimaa Elsayed, Alaa El-Gindy, Yasser El-Shabrawy, ElSayed A. Ibrahim. A Green UPLC Method for the Simultaneous determination of Tenofovir and Amoxicillin in Biological Fluids and Dosage Forms. Research Journal of Pharmacy and Technology. 2024; 17(1):74-0. doi: 10.52711/0974-360X.2024.00012   Available on: https://rjptonline.org/AbstractView.aspx?PID=2024-17-1-12


REFERENCES:
1.    Liver T. Clinical and Research Information on Drug-Induced Liver Injury [Internet]. Bethesda (MD): National Institute of Diabetes and Digestive and Kidney Diseases. 2012; Antiviral Agents; 2022 Dec 2. PMID: 31643973.
2.    Rossignol JF. "Nitazoxanide: a first-in-class broad-spectrum antiviral agent". Antiviral. 2014; Res. 110:94–103. doi:10.1016/j.antiviral.2014;07.014. PMC 7113776. PMID 25108173.
3.    Rick D. Leslie H. Nicoll. "Pharmacology – Nursing Management". Contemporary Medical-Surgical Nursing. Cengage Learning, 2011; p. 397.
4.    Sean C. Martindale — The Complete Drug Reference, 2009; Vol. 1, 36th edn, The Pharmaceutical Press, London, UK .
5.    Malipatil. S.M.  Nandedkar, M.A. Determination of Tenofovir Disoproxil Fumarate by a Sensitive Simple Isocratic RP-HPLC Method. J. Ind. Council Chem. 2009; 26 (1), 67–69.
6.    Sharma, R. Pooja A. Validated RP – HPLC Method for Simultaneous Estimation of Emtricitabine and Tenofovir Disoproxil Fumarate in a Tablet Dosage Form. Eurasian J. Anal. Chem. 2009; 4 (3), 276–284.
7.    Appala N.R. Rao V.J.  Vanitha P.K. Mukilteo, K., Srinivasu,K., Simultaneous Estimation of Tenofovir Disoproxil, Emtricitabine and Efavirenz in Tablet Dosage Form by RP- HPLC. Orient.J. Chem. 2008; 24(2), in press.
8.    Joshi M. Nikalje A.P. Shahed M. Dehghan, M. HPTLC Method for the Simultaneous Estimation of Emtricitabine and Tenofovir in Tablet Dosage Form. Ind. J. Pharmaceut. Sci. 2009; 71, 95–97.
9.    Shirkhedkar  H. Bhirud Charushila J. Surana S. Application of UV-Spectrophotometric Methods for Estimation of Tenofovir Disoproxil Fumarate in Tablets. Pak. J. Pharm. Sci. 2009; 22 (1), 27–29.
10.    Kandagal P.B. Manjunatha D.H. Seetharamappa J. Kalanur  S.S. RPHPLC Method for the Determination of Tenofovir in Pharmaceutical Formulations and Spiked Human Plasma. Anal. Lett. 2008; 41 (4), 561–570.
11.    Rezk N.L.  Crutchley  R.D. Angela D. Kashuba M. Simultaneous quantification of emtricitabine and Tenofovir in human plasma using high-performance liquid chromatography after solid phase extraction. J. Chromatogr. B. 2005; 822, 201–208.
12.    Sagar S.P. Pratik  D.  Annapurna  M.M. Stability Indicating Ion-Pair RP-HPLC Method for Estimation of Tenofovir Disoproxil Fumarate in Tablet Dosage Forms. Int. J. Pharmaceut. Res.Dev. 2009; 1, 1–9.
13.    Pires de Abreu,L.R .Mas Ortiz,R.A, HPLC determination of amoxicillin comparative bioavailability in healthy volunteers after a single dose administration. J Pharm Pharmaceut Sci. 2003; 6(2):223-230.
14.    The British Pharmacopoeia, Her Majesty’s Stationery Office, London, 2010.
15.    ICH Harmonised Tripartite Guideline, Validation of Analytical Procedures, Text and Methodology, Q2(R1), International Conference on Harmonisation, Geneva, 2005.-
16.    Płotka-Wasylka J. A new tool for the evaluation of the analytical procedure: Green Analytical Procedure Index. Talanta 2018;181:204–209.
17.    Płotka-Wasylka J. A new tool for the evaluation of the analytical procedure: Green Analytical Procedure Index. Talanta  2018;181:204–209.

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