Author(s): Gaurav Thakur, Vikas Kumar, Anuradha Sourirajan, Kamal Dev

Email(s): kamaldevbhardwaj1969@gmail.com

DOI: 10.52711/0974-360X.2023.00662   

Address: Gaurav Thakur1, Vikas Kumar1,2, Anuradha Sourirajan1, Kamal Dev1,3*
1Faculty of Applied Sciences and Biotechnology, Shoolini University of Biotechnology and Management Sciences, Bajhol, PO Sultanpur, District Solan - 173229 (H.P), India.
2Department of Biotechnology, University Institute of Biotechnology, Chandigarh University, Gharuan, Mohali, Punjab, 140413, India.
3Department of Pharmacology and Toxicology, Wright State University, Dayton, OH, 4543, USA.
*Corresponding Author

Published In:   Volume - 16,      Issue - 9,     Year - 2023


ABSTRACT:
Aegle marmelos (Rutaceae) also called as Bael is an important medicinal plant of Himalayan region. The present study aimed to evaluate the antioxidant and antimicrobial activity of different solvent extracts (n-hexane and ethanol) A. marmelos leaves from different locations of Himachal Pradesh. All the extracts were tested for the antioxidant potential by DPPH radical scavenging and FRAP assay and antimicrobial activity against Gram positive (Staphylococcus aureus), Gram negative (Escherichia coli, Klebsiella pneumoniae) bacterial and fungal strains-Candida albicans (ATCC90028) and C. albicans (MTCC277) using broth dilution method. Significant variation in antioxidant potential was observed in the ethanolic extract of A. marmelos leaves from eight different locations of Himachal Pradesh. Ethanolic extract of Mandi (M3) region showed comparatively more antioxidant potential as revealed from lower IC50 value in DPPH (14.77µg/ml) in comparison to leaves extract of other districts; whereas n hexane extract of Bilaspur (B2) showed higher FRAP activity as shown by lower IC50 value (6.62µM) as compared to leaves extracts of other districts. Antioxidant potential of ethanol extract was slightly higher than that of n-hexane for all the populations of A. marmelos. Ethanolic extracts of Sirmour district (SM1) showed lowest Minimum Inhibitory Concentration (MIC) (7.81µg/ml) against S. aureus, ethanolic extract of Mandi (M3) showed lowest MIC (7.81µg/ml) against E. coli, whereas n-hexane extracts of Chamba (CH1) and Una (U2) showed lowest MIC value (7.81µg/ml) against K. pneumonia. The n-hexane extract of Solan (SO3), ethanolic extract of Kangra (K2), ethanolic extract of Chamba (CH3) showed least MIC (31.25µg/ml) against C. albicans (ATCC90028), whereas ethanolic extracts of district Mandi (M3) showed lowest MIC (31.25 µg/ml) against C. albicans (MTCC277). From the current study it may be concluded that leaf samples of A. marmelos from Mandi district has comparatively more antioxidant potential among all other samples, whereas comparative variation in antibacterial and antifungal activity of A. marmelos leaves from different geographical regions was observed.


Cite this article:
Gaurav Thakur, Vikas Kumar, Anuradha Sourirajan, Kamal Dev. Comparative Antimicrobial and Antioxidant potential of leaf extracts of Aegle marmelos from different regions of Himachal Pradesh. Research Journal of Pharmacy and Technology 2023; 16(9):4037-1. doi: 10.52711/0974-360X.2023.00662

Cite(Electronic):
Gaurav Thakur, Vikas Kumar, Anuradha Sourirajan, Kamal Dev. Comparative Antimicrobial and Antioxidant potential of leaf extracts of Aegle marmelos from different regions of Himachal Pradesh. Research Journal of Pharmacy and Technology 2023; 16(9):4037-1. doi: 10.52711/0974-360X.2023.00662   Available on: https://rjptonline.org/AbstractView.aspx?PID=2023-16-9-6


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