Author(s): Seema Y. Mendhekar, Punit R. Rachh

Email(s): mendhekarseema41@gmail.com

DOI: 10.52711/0974-360X.2023.00711   

Address: Seema Y. Mendhekar1,2*, Punit R. Rachh1
1Department of Pharmaceutical Science, Bhagwant University, Ajmer, Rajasthan, India.
2Department of Pharmacognosy, Nagpur College of Pharmacy, Nagpur, Maharashtra, India.
*Corresponding Author

Published In:   Volume - 16,      Issue - 9,     Year - 2023


ABSTRACT:
Background: Water caltrop (Singara), a plant belonging to the Trapaceae or Lytraceae family is an essential medicinal herb used in Ayurvedic preparations. Due to its unique characteristics and therapeutic properties. It is one of the most widely consumed vegetables in Asian countries. Taiwan, China and areas of Southeast Asia are home to Singara. Objective: To study the pharmacognostic, physicochemical and preliminary phytochemical investigation of leaves of Trapa natans Linn. to establish the salient diagnostic features for the leaf. Methods: Macroscopical, microscopical, physicochemical evaluation, detection of inorganic constituents, fluorescence analysis, extractive value and phytochemical analysis were performed. Extraction was performed by using different solvent such as petroleum ether, chloroform, acetone, methanol and distill water. Results: Macroscopicaly leaves were rhomboid, fan-shaped, and serrated on the edges, measuring 2-6.5cm in diameter, broader than long, denticulate, denate, serrate, or incised with whole base, sharp apex, and thickly pubescent or villous beneath. Microscopically leaf shows abundant multicellular covering trichomes, anomocytic stomata, crystal, starch, aerenchyma cell, xylem and phloem. The result of physicochemical parameters such as foreign matter, loss on drying (moisture content), total ash, acid insoluble ash, water soluble ash, water soluble extractive value, alcohol soluble extractive value were found to be 0.2%w/w, 6.5%w/w, 8.5%w/w, 2.5%w/w, 4.5%w/w, 5.6%w/w, 3.2%w/w respectively. Qualitative tests of inorganic elements confirmed the presence of calcium, magnesium, potassium, sulphate, phosphate and carbonate. The result of fluorescence analysis showed that in visible light, the leaves powder exhibit various shades of brown and green fluorescence. Various shades of green, brown and black were found under UV light. Preliminary phytochemical investigation of various extracts using different solvents confirmed the presence of alkaloids, tannins, phenolic compounds, flavonoids, steroids, saponins, carbohydrates and glycoside respectively. Conclusion: The results of this study concluded that the presences of various phytoconstituents in Trapa natans Linn. leaves may be medicinally useful. Also the results can be helpful in setting some diagnostic indices for identification, authentication and preparation of the monograph of the leaf of the plant.


Cite this article:
Seema Y. Mendhekar, Punit R. Rachh. Pharmacognostic, Physicochemical and Preliminary Phytochemical Investigation Trapa natans Linn. Leaves. Research Journal of Pharmacy and Technology 2023; 16(9):4341-9. doi: 10.52711/0974-360X.2023.00711

Cite(Electronic):
Seema Y. Mendhekar, Punit R. Rachh. Pharmacognostic, Physicochemical and Preliminary Phytochemical Investigation Trapa natans Linn. Leaves. Research Journal of Pharmacy and Technology 2023; 16(9):4341-9. doi: 10.52711/0974-360X.2023.00711   Available on: https://rjptonline.org/AbstractView.aspx?PID=2023-16-9-55


REFERENCES:
1.    Petrovska BB. Historical Review of Medicinal Plants' usage. Pharmacognosy Reviews. 2012; 6(11): 1-5.
2.    Veeresham C. Natural products derived from plants as a source of drugs. Journal of Advanced Pharmaceutical Technology and Research. 2012; 3(4): 200–201.
3.    Hosseinzadeh, S., Jafarikukhdan, A., Hosseini, A. and Armand, R. (2015) The Application of Medicinal Plants in Traditional and Modern Medicine: A Review of Thymus vulgaris. International Journal of Clinical Medicine, 6, 635-642.
4.    Yuan H, Ma Q, Ye L, Piao G. The traditional medicine and modern medicine from natural products. Molecules. 2016; 21(5): 559.
5.    Sharma R, Kumari N, Ashawat MS, Verma CP. Standardization and phytochemical screening analysis for herbal extracts: Zingiber officinalis, Rosc., Curcuma longa Linn., Cinnamomum zeylanicum Nees., Piper longum, Linn., Boerhaavia diffussa Linn. Asian Journal of Pharmacy and Technology. 2020; 10(3): 127-33.
6.    WHO (World Health Organization) (2011) The World Traditional Medicines Situation, in Traditional medicines: Global Situation, Issues and Challenges. Geneva 3:1–14.
7.    De Luca V, Salim V, Atsumi SM, Yu F (2012) Mining the biodiversity of plants: a revolution in the making. Science 336: 1658–61. 10.1126/science.1217410.
8.    Brandao M, Cosenza GP, Pereira FL, Vasconcelos AS, Fagg CW (2013) Changes in the trade in native medicinal plants in Brazilian public markets. Environ Monit Assess 185: 7013–23. 10.1007/s10661-013-3081-y.
9.    Chen S, Yao H, Han J, Liu C, Song J, Shi L, et al. (2010) Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant species. PLoS One 5: e8613 10.1371/journal.pone.0008613
10.    Newmaster S, Ragupathy S, Janovec J (2009) A botanical renaissance: state-of-the-art DNA barcoding facilitates an Automated Identification Technology system for plants. Int J Comput Appl T 35: 1.
11.    Sucher NJ, Carles MC (2008) Genome-based approaches to the authentication of medicinal plants. Planta Med 74: 603–23. 10.1055/s-2008-1074517.
12.    Kharbanda C, Sarwar AM, Hamid H, Bano S, Haider S, Nazreen S, Javed K. Trapa natans L. root extract suppresses hyperglycemic and hepatotoxic effects in STZ-induced diabetic rat model. Journal of Ethnopharmacology. 2014; 151(2): 931–936.
13.    Majee C, Mazumder R, Choudhary AN. Pharmacognostical investigation of trapa natans l leaves. Res J Pharm Technol. 2021;14(9):4680-4684. doi:10.52711/0974-360X.2021.00813.
14.    Faruk MO, Amin MZ, Sana NK, Shaha RK, Biswas KK.Biochemical analysis of two varieties of water chestnuts (Trapa sp.). Pakistan Journal of Biological Sciences. 2012; 15 (21): 1019–1026
15.    Rodrigues RP, Aggarwal C, Saha NK. Canning of water chestnut (Singhara) (Trapa bispinosa Roxb.). Journal of Food Science and Technology.1964; 1: 28–31.
16.    Shalabh B, Akash J, Jasmine C. Trapa natans (Water Chestnut): An Overview. International Research Journal of Pharmacy. 2012; 3(6): 31–33.
17.    Khandelwal K.R. SV, Practical Pharmacognosy- Nirali Prakashan. 25 edition NP 2015: page no. 12. 1- 12.35.
18.    Rodica Bercu, Histoanatomy of the leaves of Trapa natans (Trapaceae), PHYTOLOGIA BALCANICA 10 (1): 51–55, Sofifi a, 2004.
19.    Surabi S, Krishnan V. Phytochemical and elemental analysis of Jasminum cuspidatum (L.). Surabi et al World Journal of Pharmaceutical Research. 2018;7:943. doi:10.20959/wjpr20186-11481
20.    The ayurvedic pharmacopoeia of India Part-II Volume-I Government of India ministry of health and family welfare department of ayush, First edition,2007:Page:141.
21.    Panda D, Kumar Dash S, Kumar Dash G. Preliminary phytochemical screening and Pharmacogcostic studies of the common weeds, Mimosa pudica Linn., Chromolaena odorata Linn. and Tragia involucrata Linn. Research Journal of Pharmacognosy and Phytochemistry. 2012;4(2):92-96.
22.    Indian Pharmacopoeia 2007 Volume 1 The Indian Pharmacopoeia Commission Ghaziabad.; 2007. www.ipc.gov.in
23.    World Health Organization. Pharmaceuticals Unit. Quality control methods for medicinal plant materials. Geneva : World Health Organization. Published online 1998:122.
24.    Khandelwal K.R. SV, Practical Pharmacognosy- Nirali Prakashan. 25 edition NP 2015: page no. 25.8-29.9.
25.    Chase CR, Pratt R. Fluorescence of powdered vegetable drugs with particular reference to development of a system of identification. Journal of the American Pharmaceutical Association. 1949; 38: 324-331
26.    Gaikwad S, Mohan GK, Reddy K. Proximate analysis and preliminary phytochemical standardization of leaves of two Malvaceous Plants. Research Journal of Pharmacognosy and Phytochemistry. 2011; 3(5): 211-6.
27.    Bahadur S, Roy A, Chanda R, Baghel P, Saha S, Choudhury A. Extraction and evaluation of some phytochemcial and physicochemical properties of Hibiscus rosasinensis mucilage. Research Journal of Pharmacognosy and Phytochemistry. 2018(4): 205-8.
28.    Kaushik R, Jain J, Rai P, Sharma Y, Kumar V, Gupta A. Pharmacognostical, physicochemical and preliminary phytochemical studies of Anthocephalus cadamba (Roxb.) Leaves. Research Journal of Pharmacy and Technology. 2018; 11(4): 1391-7.
29.    Khandelwal K.R. SV, Practical Pharmacognosy- Nirali Prakashan. 25 edition NP 2015: page no. 25.1-25.6.

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