Author(s): Asha Mary John, Anjana Baby, Asmin Juberia Rahim, Besteena Baby, Elsa Babu

Email(s): ashabenish@gmail.com

DOI: 10.52711/0974-360X.2023.00696   

Address: Asha Mary John1*, Anjana Baby2, Asmin Juberia Rahim2, Besteena Baby2, Elsa Babu2
1MGM Silver Jubilee College of Polytechnic and Pharmaceutical Sciences, Department of Pharmacology, Pampakuda, Ernakulam, Kerala, India.
2Chemists College of Pharmaceutical Sciences and Research, Department of Pharmacology, Ernakulam, Kerala, India.
*Corresponding Author

Published In:   Volume - 16,      Issue - 9,     Year - 2023


ABSTRACT:
The purpose of this study is to assess the hypolipidemic potential of formulated suspensions of atorvastatin, alcoholic extract of Murraya koenigii and their combination on hyperlipidemic Wistar rats. Hyperlipidemia was induced in all groups except negative control group, using High Fat Diet (HFD). Formulated suspensions of Murayya koenigii leaf extract, atorvastatin and its combination were administered to HFD fed rats for 28 days. Treatment related changes in body weights were recorded. Extraction of Murraya koenigii was carried out by maceration. After completion of treatments, blood samples were collected immediately and serum separated was used for the estimation of lipid profile and glucose levels. Treatment with all the formulated suspensions shows significant normalization of body weight compared with positive control group (62.5±3.14) in HFD fed animals. The effect of combined suspension was more pronounced (40.08±4.86) and shows significant reduction in body weight gain, P<0.01 than that of Murraya koenigii leaf extract (57.94 ±4.36) or atorvastatin suspensions (45.97± 4.48) alone. Combined suspension also exhibits significant reduction, P<0.001 in serum TC, TG, LDL and VLDL. It also enhanced the cardio protective lipid HDL after 28 days of treatment. A significant reduction in serum glucose levels were noted with suspension of Murraya koenigii leaf extract alone, P<0.05 compared with positive control. It is possible to draw the conclusion from the current study that combining atorvastatin and Murraya koenigii leaf extract in a suspension may have had a synergistic effect on lowering body weight and hypolipidemic activity.


Cite this article:
Asha Mary John, Anjana Baby, Asmin Juberia Rahim, Besteena Baby, Elsa Babu. Evaluation of Synergistic Hypolipidemic Activity of Murraya koenigii and Atorvastatin suspension in Hyperlipidemic rats. Research Journal of Pharmacy and Technology 2023; 16(9):4251-6. doi: 10.52711/0974-360X.2023.00696

Cite(Electronic):
Asha Mary John, Anjana Baby, Asmin Juberia Rahim, Besteena Baby, Elsa Babu. Evaluation of Synergistic Hypolipidemic Activity of Murraya koenigii and Atorvastatin suspension in Hyperlipidemic rats. Research Journal of Pharmacy and Technology 2023; 16(9):4251-6. doi: 10.52711/0974-360X.2023.00696   Available on: https://rjptonline.org/AbstractView.aspx?PID=2023-16-9-40


REFERENCE:
1.    Duan Y, Zeng L, Zheng C, Song B, Li F, Kong X, Xu K. Inflammatory Links Between High Fat Diets and Diseases. Frontiers in Immunology. 2018; 9: 2649. https://doi.org/10.3389/fimmu.2018.02649
2.    M. Selvakumar, Vijayalakshmi Chinniah, Venkata Rathina Kumar Thiagarajan. Antiobesity Activity of Ficus religiosa on High Fat Diet Induced Model. Research J. Pharm. and Tech. 2015; 8(6): 679-682. doi: 10.5958/0974-360X.2015.00107.9
3.    T. Sudha1, D. Akila Devi, L. Kaviarasan. Antihyperlipidemic effect of Stevia rebaudiana on Alloxan Induced Diabetic Rats. Asian J. Pharm. Tech. 2017; 7 (4):202-208. doi: 10.5958/2231-5713.2017.00031.9
4.    Mawhoob N. Alkadasi, Abdulsalam Mohammed Alshami, H.Y. Alhabal. Study the Relation of Serum Lipids with Body Mass Index Among Students in Zabeed Education Collage, Hudaiadah University, Yemen. Asian J. Pharm. Ana. 2015; 5(1): 31-35. doi: 10.5958/2231-5675.2015. 00006.X
5.    Ch. Nagabhushanam, P Ramesh babu, NK Durga Devi, A Vasu, Devala Rao. A Comparative Randomized Study of Guggulu and Atorvastatin Hypercholesterolemia Patients. Research J. Pharmacology and Pharmacodynamics. 2010; 2(2):187-189.
6.    Preeti Tiwari. Antihyperlipidemic Potential of Balarishta Prepared by Traditional and Modern Methods in High Fat Diet Induced Hyperlipidemic rats. Asian J. Res. Pharm. Sci. 2014; 4(1): 07-11.
7.    Paras Virani, Rajanit Sojitra, Hasumati Raj, Vineet Jain. Atorvastatin: A Review on Analytical Method and Its Determination in Pharmaceuticals and Biological Matrix. Asian J. Pharm. Ana. 2015; 5(3): 151-160. doi: 10.5958/2231-5675.2015.00025.3
8.    Nitin Mahurkar, S.M Sayeed ul hasan, S. Mutaal Quadri. Antihyperlipidemic Effect of Polyherbal Formulation (PHF) in High Fat Diet Induced Hyperlipidemia. Res. J. Pharm. Dosage Form. and Tech. 2015; 7(1): 11-14. doi: 10.5958/0975-4377.2015.00003.8
9.    Iyer UM, Mani UV. Studies on the effect of curry leaves supplementation (Murraya koenigii) on lipid profile, glycated proteins and amino acids in non-insulin dependent diabetic patients. Plant Foods for Human Nutrition.1990; 40(4):275-282. doi: 10.1007/BF02193851
10.    Molly J, Edison S, Vijajaraghavan R, Ajith TA. Effect of curry leaves and cucumber fruit on lipid profile in menopausal women with hyperlipidemia: a randomized controlled pilot study. Int J Clin Trials. 2017;4(1):7-13. https://dx.doi.org/10.18203/2349-3259.ijct20164059
11.    Angelidi AM, Stambolliu E, Adamopoulou KI, Kousoulis AA. Is Atorvastatin Associated with New Onset Diabetes or Deterioration of Glycemic Control; Systematic Review Using Data from 1.9 million patients. International Journal of Endocrinology. 2018; Article ID 8380192,1-17. https://doi.org/10.1155/2018/8380192
12.    Abubakar AR, Haque M. Preparation of Medicinal Plants: Basic Extraction and Fractionation Procedures for Experimental Purposes. Journal of Pharmacy and Bioallied Sciences. 2020; 12(1):1-10. doi: 10.4103/jpbs.JPBS_175_19
13.    Kalaivanan S, Sarumathy S, Anisha Ebens J, Naresh Kumar K, Roobena Parveen A, Nasreen Ashraf M.Clinical Assessment and Comparison of Lipid Profiles among Coronary Artery Disease and type 2 Diabetes Mellitus Patients receiving Statin Therapy. Research J. Pharm. and Tech. 2017; 10(1): 18-20. doi: 10.5958/0974-360X.2017.00005.1
14.    Khandelwal K R, Practical Pharmacognosy Techniques and Experiments, 18th edition., Nirali Prakashan, 2007: 153.
15.    Donalda RM, Camargo SS, Meyre-Silvaa C,Meira Quintãoa NL,Filhoa VC, Bresolina TMB,Lucinda-Silva RM. Development of an oral suspension containing dry extract of Aleurites moluccanus with anti-inflammatory activity. Brazilian Journal of Pharmacognosy. 2015; 26: 68–76. http://dx.doi.org/10.1016/j.bjp.2015.06.011
16.    Santoshkumar J, Manjunath S, Pranavkumar SM. A study of anti-hyperlipidemia, hypolipedimic and anti-atherogenic activity of fruit of Emblica officinalis (Amla) in high fat fed albino rats. International Journal of Medical Research and Health Sciences. 2013 ;2(1):70-77.
17.    Shyamala M, Venukumar M, Mukalel L. Antioxidant potential of the Syzygium aromaticum (Gaertn.) Linn. (cloves) in rats fed with high fat diet. Indian J. Pharmacol. 2003; 35.
18.    Sikarwar MS, Patil MB. Antihyperlipidemic activity of Salacia chinensis root extracts in triton-induced and atherogenic diet-induced hyperlipidemic rats. Indian J Pharmacol. 2012; 44:88-92. doi: 10.4103/0253-7613.91875.
19.    Boizel R, Laporte F, Benhamou YP, Foulon T, Lardy B, Halimi S. Ratio of Triglycerides to HDL Cholesterol as an Indicator of LDL Particle Size in Patients with Type 2 Diabetes and Normal HDL Cholesterol Levels. Diabetes Care. 2000; 23(11): 1679-85. doi: 10.2337/diacare.23.11.1679.
20.    Kumar VS, Sharma A, Tiwari R, Kumar S, Murraya koenigii. (Curry leaf): a review. Journal of Medicinal and Aromatic Plant Science. 1999; 21(4):1139-1141.
21.    Gahlawat DK, Jakhar S,Dahiya P. Murraya koenigii. (L.) Spreng: an ethnobotanical, phytochemical and pharmacological review. Journal of Pharmacognosy and Phytochemistry. 2014; 3(3):109-119.
22.    Chandra BV, Buchineni M, Kudagi BL, Mohan PR, Haritha M, Devi NA. Hypolipidemic activity of Murraya koenigii in Alloxan induced Diabetic rats. The Pharma Innovation Journal. 2016; 5(5): 101-103.
23.    Mani DN, Bawankule DU, Saroj BK. Hyperlipidemic model: studying lipid profile in small experimental animal. Int J Pharm Pharm Sci. 2012, 4(3): 337-340.
24.    Harrison D, Kathy V, Horing B, Drexler H. Role of oxidative stress in atherosclerosis. American Journal of Cardiology. 2003;91, 7A–11A. doi: 10.1016/s0002-9149(02)03144-2
25.    Wilson PWF. High density lipoprotein, low density lipoprotein and coronary heart disease. American Journal of Cardiology. 1990; 66, 7A–10A. doi: 10.1016/0002-9149(90)90562-f.
26.    Ganda OP. Statin-induced diabetes: incidence, mechanisms, and implications. F1000Research.2016; 5, F1000 Faculty Rev-1499. doi: 10.12688/f1000research.8629.1.
27.    Tembhurne SV, Sakarkar DM. Anti-obesity and hypoglycemic effect of ethanolic extract of Murraya Koenigii (L) leaves in high fatty diet rats. Asian Pacific Journal of Tropical Disease, s166-s168 (2012). DOI:10.1016/S2222-1808(12)60145-5
28.    Baskaran C, Bai VR, Kanimozhi D. Screening for Antimicrobial Activity and Phytochemical Analysis of Various Leaf Extract of Murraya koenigii. International Journal of Research in Ayurveda and Pharmacy. 2011;2(6): 1807-10.
29.    R Namitha, J S Ampily. Phytochemical analysis and antimicrobial screening of Murayya koenigii Extracts. Res. J. Pharmacognosy and Phytochem. 2020; 12(2): 101-105. doi: 10.5958/0975-4385.2020.00017.5
30.    A. Sowmya, T. Ananthi. Hypolipidemic activity of Mimosa pudica Linn on Butter Induced Hyperlipidemia in Rats. Asian J. Res. Pharm. Sci. 1(4): Oct.-Dec. 2011; Page 123-126.
31.    Vinarova L, Vinarov Z, Atanasov V, Pantcheva I, Tcholakova S, Denkov N. Lowering of cholesterol bioaccessibility and serum concentrations by saponins: In vitro and in vivo studies. Food Funct. 2015;6(2):501-12. doi: 10.1039/c4fo00785a
32.    Li H, Wang QJ, Zhu DN, Yang Y. Reinioside C. A triterpene saponin of Polygala aureocauda Dunn, exerts hypolipidemic effect on hyperlipidemic mice. Phytother Res. 2008; 22 (2): 159-164. doi: 10.1002/ptr.2262
33.    Stancu C, Sima, A. Statins: mechanism of action and effects. Journal of Cellular and Molecular Medicine. 2001;5(4), 378–387. doi: 10.1111/j.1582-4934. 2001.tb00172.x

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

1.3
2021CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags


Not Available