Author(s): Kose Jackalas, Elizabeth Mary Mathew

Email(s): ,

DOI: 10.52711/0974-360X.2023.00658   

Address: Kose Jackalas, Elizabeth Mary Mathew
School of Pharmacy, Faculty of Health Sciences, University of Botswana, Plot 4775 Notwane Rd, Gaborone, Botswana.
*Corresponding Author

Published In:   Volume - 16,      Issue - 9,     Year - 2023

Searsia lancea (SL) has been exploited by native healers in the Southern African region for the treatment of various bacterial and fungal infections. This study was aimed to analyze the antimicrobial activity and the minimum inhibitory concentration of SL root extract against Candida albicans, Candida glabrata, E.coli and Staph aureus. The maceration technique was used as the extraction method. The antifungal and antibacterial activity was determined by the agar well diffusion method. The agar dilution method was used for estimating the minimum inhibitory concentration (MIC). The biological investigations in this study clearly demonstrate the antibacterial and antifungal potential of SL aqueous and organic extract. The aqueous and organic Sersia Lancea root extract achieved zone of inhibition from 17.1-40.2mm against the tested organisms. The order of susceptibility of the pathogens to the tested extracts was found to be S.aureus>Candida Albicans>E.coli>Candida Glabrata. The results of the MIC experiment demonstrate that Candida Albicans and E.Coli were susceptible to the MIC of 3.12mg/mL for the SL distilled water root extract . The MIC of the aqueous extract against Candida glabrata and Staph Aureus were 6.25 and 12.5mg/mL respectively. All the microrgaisms were susceptible to MIC 6.25mg/mL for the DCM extract except for Candida Glabrata with MIC of 12.5mg/mL. The current work has not performed any isolation or structural elucidation studies for the potential compounds responsible for the antimicrobial activity. This is the first report to demonstrate the antibacterial and antifungal property of SL root extract. The results in this work provide scope for future studies to elucidate the structure of potential actives in SL root extract and to test their potential as possible antibacterial and antifungals.

Cite this article:
Kose Jackalas, Elizabeth Mary Mathew. In vitro Evaluation of Sersia Lancea Root Extract for the treatment of Common Microbial Infections. Research Journal of Pharmacy and Technology 2023; 16(9):4016-0. doi: 10.52711/0974-360X.2023.00658

Kose Jackalas, Elizabeth Mary Mathew. In vitro Evaluation of Sersia Lancea Root Extract for the treatment of Common Microbial Infections. Research Journal of Pharmacy and Technology 2023; 16(9):4016-0. doi: 10.52711/0974-360X.2023.00658   Available on:

1.    Moellering Jr RC. Discovering new antimicrobial agents. International Journal of Antimicrobial Agents. 2011; 37(1):2-9.doi: 10.1016/j.ijantimicag.2010.08.018
2.    Tarange R G, Kharat J S, Jedage H D, Manjunath K P. Phytochemical screening for Antioxidant and Antifungal activity of Lagenaria siceraria (Molina) Standl. leaf extract. Res. J. Pharmacognosy and Phytochem. 2019; 11(3):155-159. doi: 10.5958/0975-4385.2019.00026.8
3.    Punet Kumar, Sangam, Nitin Kumar. Pharmacognostical, Pharmacological Studies and Traditional uses of Plectranthus Ambonicus: A Review. Res. J. Pharmacognosy and Phytochem. 2019; 11(4):244-250. doi: 10.5958/0975-4385.2019.00041.4
4.    Swati Patil, S Prabhu, CI Jolly, S Narayanan. Antibacterial and Anti-Diarrhoel Activities of Flowers of Punica granatum Linn. Research J. Pharmacology and Pharmacodynamics. 2009; 1(2): 87-89.
5.    Anil G Markandeya, Narayan P Firke, Suneeti S Gore, Sunita Salunke Gawali, Shirish S Pingale. Antibacterial Activity of Celocia argentea Leaves Extract in Organic Solvents. Research Journal of Pharmacology and Pharmacodynamics. 2014; 6(2): 79-81.
6.    Van Wyk BE, Oudtshoorn BV, Gericke N. Medicinal Plants of South Africa. Pretoria (South Africa): Briza;1997.
7.    Kabongo‐Kayoka PN, Eloff JN, Obi CL, McGaw LJ. Antimycobacterial activity and low cytotoxicity of leaf extracts of some African Anacardiaceae tree species. Phytotherapy Research. 2016; 30 (12):2001-2011. doi: 10.1002/ptr.5706.
8.    Rayne S, Mazza G. Biological Activities of Extracts from Sumac (Rhus spp.): A Review. Plant foods for Human Nutrition . 2007;62(4):165-75. doi: 10.1007/s11130-007-0058-4.
9.    Gundidza M, Gweru N, Mmbengwa V, Ramalivhana NJ, Magwa Z, Samie A. Phytoconstituents and biological activities of essential Oil from Rhus lancea L. F. African Journal of Biotechnology. 2008;7(16): 2787-2789.
10.    Mulaudzi RB, Ndhlala AR, Kulkarni MG, Van Staden J. Pharmacological properties and protein binding capacity of phenolic extracts of some Venda medicinal plants used against cough and fever. Journal of Ethnopharmacology. 2012;143(1):185-93. doi: 10.1016/j.jep.2012.06.022.
11.    Vambe M, Aremu AO, Chukwujekwu JC, Finnie JF, Van Staden J. Antibacterial screening, synergy studies and phenolic content of seven South African medicinal plants against drug-sensitive and-resistant microbial strains. South African Journal of Botany. 2018;114:250-259. doi:10.1016/j.sajb.2017.11.011.
12.    Fouché G, Cragg GM, Pillay P, Kolesnikova N, Maharaj VJ, Senabe J. In vitro anticancer screening of South African plants. Journal of Ethnopharmacology. 2008; 28;119(3):455-61. doi: 10.1016/j.jep.2008.07.005.
13.    Chaman S, Sharma G, Reshi AK. Study of antimicrobial properties of Catharanthus roseus by agar well diffusion method.International Journal of Pharmacy and Pharmaceutical Sciences. 2013;3(5):65-68.
14.    Punasiya R, Dindorkar G, Pillai S. Antibacterial and Antifungal Activity of Flower extract of Murraya paniculata L. Asian J. Res. Pharm. Sci. 2020; 10(1):17-20. doi: 10.5958/2231-5659.2020.00004.1
15.    Sonu Jacob, Anil John J., Leena Thomas, Sabulal B. In vitro pharmacological activity of the whole plant Naregamia alata. Asian J. Research Chem. 2011; 5(2);265-269.
16.    K. G. Purushotham, P. Arun, J. Johnsy Jayarani, R. Vasanthakumari, D. Chamundeeswari. In Vitro Antibacterial and Antifungal Assay of Tectona grandis-A Screening Study. Research J. Pharmacognosy and Phytochemistry.  2010; 2(1): 57-60.
17.    Griffin SG, Markham JL, Leach DN. An agar dilution method for the determination of the minimum inhibitory concentration of essential oils. Journal of Essential Oil Research. 2000; 12:249-255.
18.    Rej S, Dutta M, Jamal S, Das S, Chatterjee S. Study of phytochemical constituents and antibacterial activity of Clerodendrum infortunatum. Asian Journal of Research in Pharmaceutical Science. 2014 ;4(4):187-95.
19.    Jaiganesh KP, Sreedharren B, Arunachalam G, Nirmala R, Nepolean R. Pharmacognostical and Antimicrobial investigation of Jatropha curcas, Linn., Leaf. Alcohol. 2013 ;17:7.
20.    Naidu N, Kumar GS, Sivakrishna K, Anjinaik K, Kumar LP, Sneha G. Anti microbial and antioxidant evolution of aqueous extract of Terminalia chebula using disc diffusion, H 2 O 2 scavenging methods. Proteus. 2017;7(2): 112-114. doi: 10.5958/2231-5659.2017.00017.0
21.    Daniel MM, Shimane WM. Medicinal and edible wild fruit plants of Botswana as emerging new crop opportunities. Journal of Medicinal Plant Research. 2011; 5(10):1836-1842.
22.    Vambe M, Naidoo D, Aremu AO, Finnie JF, Van Staden J. Bioassay-guided purification, GC-MS characterization, and quantification of phyto-components in an antibacterial extract of Searsia lancea leaves. Natural Product Research. 2021; 35(22): 4658-466.10.1080/14786419.2019.1700251.
23.    Mudzengi CP, Murwira A, Tivapasi M, Murungweni C, Burumu JV, Halimani T. Antibacterial activity of aqueous and methanol extracts of selected species used in livestock health management. Pharmeutical Biology. 2017; 55(1): 1054-1060. doi: 10.1080/13880209.2017.1287744.

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

56th percentile
Powered by  Scopus

SCImago Journal & Country Rank

Recent Articles


Not Available