Author(s): Amber Vyas, Vishal Jain, Umakant Sahu, Narendra Kumar, Neelu Joshi


DOI: 10.52711/0974-360X.2023.00652   

Address: Amber Vyas1, Vishal Jain1*, Umakant Sahu1, Narendra Kumar1, Neelu Joshi2
1University Institute of Pharmacy, Pt. Ravishankar Shukla University Raipur, Chhattisgarh.
2School of Biotechnology and Bioinformatics, D. Y. Patil Deemed To Be University, Navi Mumbai.
*Corresponding Author

Published In:   Volume - 16,      Issue - 8,     Year - 2023

High Performance Thin Layer Chromatography (HPTLC) is the most potent and sophisticated type of Thin Layer Chromatography (TLC). It uses chromatographic layers with the highest levels of separation, efficiency and employs high-tech equipment for every step of the process, including accurate sample application, standardised reproducible chromatogram development, and software-controlled evaluation. HPTLC is a concept that incorporates both the use of established methodologies for qualitative and quantitative analysis and a widely standardised methodology founded on scientific facts. The resolution can be increased and more exact quantitative measurements, which satisfies all quality standards for today's analytical needs. Development of an analytical technique based on HPTLC and parameter validation in line with practical assessment. It complies with standards while reducing mistakes and inquiries. Quality Control and Quality Assurance of raw materials of Plant Origin can easily and effectively be done qualitative characterization and quantitative determination of mixtures of substances, Chemical Fingerprinting by High Performance Liquid Chromatography. This review article provides fundamental principles, guidance for proper validation practise, aids in selecting the best mobile phase, and clarifies the processes of the analytical process., protocol, separation, resolution, validation process, current advancements, changes made to TLC that led to HPTLC, optimization, process control, automation, and hyphenation.

Cite this article:
Amber Vyas, Vishal Jain, Umakant Sahu, Narendra Kumar, Neelu Joshi. HPTLC Method Development of Herbal drugs and its Validation: An Overview. Research Journal of Pharmacy and Technology. 2023; 16(8):3964-6. doi: 10.52711/0974-360X.2023.00652

Amber Vyas, Vishal Jain, Umakant Sahu, Narendra Kumar, Neelu Joshi. HPTLC Method Development of Herbal drugs and its Validation: An Overview. Research Journal of Pharmacy and Technology. 2023; 16(8):3964-6. doi: 10.52711/0974-360X.2023.00652   Available on:

1.    Farag MA, El Senousy AS, El-Ahmady SH, Porzel A, Wessjohann LA. Comparative metabolome-based classification of Senna drugs: a prospect for phyto-equivalency of its different commercial products. Metabolomics. 2019;15(5):1-10. doi:10.1007/s11306-019-1538-x
2.    Rahman S, Ul Haq F, Ali A, et al. Combining untargeted and targeted metabolomics approaches for the standardization of polyherbal formulations through UPLC–MS/MS. Metabolomics. 2019;15(9):1-11. doi:10.1007/s11306-019-1582-6
3.    Leong F, Hua X, Wang M, et al. Quality standard of traditional Chinese medicines: Comparison between European Pharmacopoeia and Chinese Pharmacopoeia and recent advances. Chinese Med (United Kingdom). 2020;15(1):1-20. doi:10.1186/s13020-020-00357-3
4.    Yu SM, Kim SJ, Yoon YC, Kim JH. Development and application of a chemical profiling method for the assessment of the quality and consistency of the Pelargonium sidoides extract. J Anal Sci Technol. 2021;12(1). doi:10.1186/s40543-021-00297-z
5.    Olefir Y V., Sakanyan EI, Shishova LI, Senchenko SP. Pharmacopoeial Quality Requirements for Medicinal Products. Pharm Chem J. 2019;52(11):936-941. doi:10.1007/s11094-019-01929-w
6.    Kowalska T. Botanicals – Its Versatile Potential and Selected Applications. Molecules. 2022;27(19):6607.
7.    Wei XC, Cao B, Luo CH, et al. Recent advances of novel technologies for quality consistency assessment of natural herbal medicines and preparations. Chinese Med (United Kingdom). 2020;15(1):1-24. doi:10.1186/s13020-020-00335-9
8.    Attimarad M, Mueen Ahmed KK, Aldhubaib BE, Harsha S. High-performance thin layer chromatography: A powerful analytical technique in pharmaceutical drug discovery. Pharm Methods. 2011;2(2):71-75. doi:10.4103/2229-4708.84436
9.    Upton R, David B, Gafner S, Glasl S. Botanical ingredient identification and quality assessment: strengths and limitations of analytical techniques. Phytochem Rev. 2020;19(5):1157-1177. doi:10.1007/s11101-019-09625-z
10.    Upton RT. Use of high-performance thin layer chromatography by the American Herbal Pharmacopoeia. J AOAC Int. 2010;93(5):1349-1354. doi:10.1093/jaoac/93.5.1349
11.    Siddiqui MR, AlOthman ZA, Rahman N. Analytical techniques in pharmaceutical analysis: A review. Arab J Chem. 2017;10:S1409-S1421. doi:
12.    Morozov S V., Tkacheva NI, Tkachev A V. On Problems of the Comprehensive Chemical Profiling of Medicinal Plants. Russ J Bioorganic Chem. 2019;45(7):860-875. doi:10.1134/S1068162019070070
13.    Balekundri A, Mannur V. Quality control of the traditional herbs and herbal products: a review. Futur J Pharm Sci. 2020;6(1). doi:10.1186/s43094-020-00091-5
14.    World Health Organization (WHO). WHO Traditional Medicine Strategy 2014-2023. World Heal Organ. Published online 2013:1-76. doi:2013
15.    Gupta R MA. Fingerprint Profile of Selected Ayurvedic Churnas/Preparations: An Overview. Altern Integr Med. 2013;02(06). doi:10.4172/2327-5162.1000125
16.    Singh S, Kumar A, Semwal BC. PRISMA Based Systematic Review: Pharmacognostic Study of Zanthoxylum armatum DC. Mini Rev Med Chem. 2021;21(14):1965-1997. doi:10.2174/1389557521999201230200503
17.    Arora P, Deol RK, Jena A, Kapoor VK. Quantitative Determination of Berberine in Marketed Herbal Formulations by TLC Densitometry and their Hypoglycemic Effect. Int J Pharmacogn Phytochem Res. 2018;9(11):1398-1404. doi:10.25258/phyto.v9i11.11181
18.    Woo CSJ, Lau JSH, El-Nezami H. Herbal Medicine. Toxicity and Recent Trends in Assessing Their Potential Toxic Effects. Vol 62. 1st ed. Elsevier Ltd.; 2012. doi:10.1016/B978-0-12-394591-4.00009-X
19.    Calixto JB. Twenty-five years of research on medicinal plants in Latin America: a personal view. J Ethnopharmacol. 2005;100(1-2):131-134. doi:10.1016/j.jep.2005.06.004
20.    Srirama R, Santhosh Kumar JU, Seethapathy GS, et al. Species Adulteration in the Herbal Trade: Causes, Consequences and Mitigation. Drug Saf. 2017;40(8):651-661. doi:10.1007/s40264-017-0527-0
21.    W.H.O. Traditional Medicine Strategy (2002–2005). WHO/EDM/TRM/2002.1. Published online 2002.
22.    Zade SR, Priya BL, Bagmar UR, Ganjiwale RO. Development and validation of HPTLC method for estimation of hepatoprotective diterpenoid andrographolide in polyherbal formulations. In: ; 2013.
23.    Alam P, Yusufoglu H, Alam A. HPTLC densitometric method for analysis of thymoquinone in Nigella sativa  extracts and marketed formulations. Asian Pacific J Trop Dis. 2013;3(6):467-471. doi:10.1016/S2222-1808(13)60102-4
24.    Dhalwal K, Shinde VM, Namdeo AG, Mahadik KR, Kadam SS. Development and validation of a TLC-densitometric method for the simultaneous quantitation of strychnine and brucine from Strychnos spp. and its formulations. J Chromatogr Sci. 2007;45 10:706-709. Ministry of Ayush, Govt. of India. The Ayurvedic Pharmacopoeia of India. First Edit. Pharmacopoeia Commission for Indian Medicine and Homoeopathy Ghaziabad; 2016.
25.    WHO. Quality control methods for medicinal plant materials World Health Organization Geneva. Who. Published online 1998. doi:10.1016/j.pbi.2015.01.003
26.    Rybak-Chmielewska H. Honey. Chem Funct Prop Food Saccharides. 2003;1(Volume 1):73-80. doi:10.7312/seir17116-004
27.    S. C. VERMA, C. L. JAIN, R. RANI, et al. Simple and Rapid Method for Identification of Curcuma Longa Rhizomes by Physicochemical and HPTLC Fingerprint Analysis. Chem Sci Trans. 2012;1(3):709-715. doi:10.7598/cst2012.203
28.    Pundarikakshudu K, Dave HN. Simultaneous Determination of Curcumin and Berberine in their Pure Form and from the Combined Extracts of Curcuma Longa and Berberis Aristata. Int J Appl Sci Eng. 2010;8(1):19-26. doi:10.6703/IJASE.2010.8(1).19
29.    Thorat N, Dodiya T, Prajapati D. HPTLC Method Development and Validation for Simultaneous Estimation of Berberine, Ellagic Acid and Ferulic Acid in Amrtadi Churna. J Nat Remedies. 2022;22(4):649-657. doi:10.18311/jnr/2022/31350
30.    Harron DWG. Technical Requirements for Registration of Pharmaceuticals for Human Use: The ICH Process. Textb Pharm Med. 2013;1994(November 1996):447-460. doi:10.1002/9781118532331.ch23
31.    Srivastava M. High-performance thin-layer chromatography (HPTLC). High-Performance Thin-Layer Chromatogr. Published online 2011:1-397. doi:10.1007/978-3-642-14025-9
32.    Shrivastava A, Gupta V. Methods for the determination of limit of detection and limit of quantitation of the analytical methods. Chronicles Young Sci. 2011;2(1):21. doi:10.4103/2229-5186.79345
33.    Folcará SC, Frommenwiler DA, Reich E, Casanovas RV. High performance thin-layer chromatography (HPTLC) in the quality control of herbal products. In: ; 2018.
34.    WHO. WHO guidelines on good agricultural and collection practices (GACP) for medicinal plants. World Health. Published online 2003:80.
35.    Yuan X, Chapman RL, Wu Z. Analytical methods for heavy metals in herbal medicines. Phytochem Anal. 2011;22(3):189-198. doi:10.1002/pca.1287
36.    Saper RB, Kales SN, Paquin J, et al. Heavy metal content of ayurvedic herbal medicine products. JAMA. 2004;292(23):2868-2873. doi:10.1001/jama.292.23.2868
37.    Liu SY, Woo SO, Koh HL. HPLC and GC-MS screening of Chinese proprietary medicine for undeclared  therapeutic substances. J Pharm Biomed Anal. 2001;24(5-6):983-992. doi:10.1016/s0731-7085(00)00571-9
38.    Ekor M. The growing use of herbal medicines: issues relating to adverse reactions and  challenges in monitoring safety. Front Pharmacol. 2014;4:177. doi:10.3389/fphar.2013.00177
39.    Kamboj A. Analytical Evaluation of Herbal Drugs. Drug Discov Res Pharmacogn. Published online 2012. doi:10.5772/26109
40.    Sumbul S, Ahmad MA, Asif M, Akhtar M, Saud I. Physicochemical and phytochemical standardization of berries of Myrtus communis Linn. J Pharm Bioallied Sci. 2012;4(4):322-326. doi:10.4103/0975-7406.103266
41.    Liu J, Zhang F, Ravikanth V, Olajide OA, Li C, Wei LX. Chemical Compositions of Metals in Bhasmas and Tibetan Zuotai Are a Major Determinant of Their Therapeutic Effects and Toxicity. Capasso R, ed. Evidence-Based Complement Altern Med. 2019;2019:1697804. doi:10.1155/2019/1697804
42.    Byard RW. A review of the potential forensic significance of traditional herbal medicines. J Forensic Sci. 2010;55(1):89-92. doi:10.1111/j.1556-4029.2009.01252.x
43.    Etkin NL. Quality Standards of Indian Medicinal Plants, Volume 1. J Ethnopharmacol. 2004;90(2-3):422. doi:10.1016/j.jep.2003.10.015
44.    Wagner H and B. Wagner, H. and Bladt, S. (1996) Plant Drug Analysis: A Thin Layer Chromatography Atlas. 2nd Edition, Springer-Verlag, Berlin.; 1996.
45.    Sunil Kumar KN, Pushpendra P, Holla BS, Ravishankar B, Yashovarma B. High performance thin layer chromatography qualitative densitometry as a  sensitive method to assess shelf life of polyherbal formulations: A study on Hutabhugadi Churna. Ayu. 2015;36(3):303-310. doi:10.4103/0974-8520.182745
46.    Sumbul S, Ahmad MA, Asif M, Akhtar M, Saud I. Physicochemical and phytochemical standardization of berries of Myrtus communis  Linn. J Pharm Bioallied Sci. 2012;4(4):322-326. doi:10.4103/0975-7406.103266
47.    Muyumba NW, Mutombo SC, Sheridan H, Nachtergael A, Duez P. Quality control of herbal drugs and preparations: The methods of analysis, their relevance and applications. Talanta Open. 2021;4. doi:10.1016/j.talo.2021.100070
48.    Bandaranayake WM. Quality control, screening, toxicity and regulations of herbal drugs. In: ; 2006.
49.    Yadav NP, Plants A, Dixit V. Recent Approaches in herbal drug standardization Recent Approaches in Herbal Drug Standardization Herbal drug standardization. 2014;(May).
50.    World Health Organization (WHO). World Health Organization, 2011. Quality control methods for herbal materials. World Health Organization. Published online 2011.
51.    Manilal A, Sabu KR, Woldemariam M, et al. Antibacterial Activity of Rosmarinus officinalis against Multidrug-Resistant Clinical Isolates and Meat-Borne Pathogens. Yang Q, ed. Evidence-Based Complement Altern Med. 2021;2021:6677420. doi:10.1155/2021/6677420
52.    Ristivojević P, Trifković J, Vovk I, Milojković-Opsenica D. Comparative study of different approaches for multivariate image analysis in HPTLC fingerprinting of natural products such as plant resin. Talanta. 2017;162(July 2016):72-79. doi:10.1016/j.talanta.2016.10.023
53.    Pandya H, Sharma R, Prajapati P. Standardization and quality control parameters of dashmoola kwatha Granules for instant use : an ayurvedic formulation. 2014;(January).
54.    Abo-Gharam AH, El-Kafrawy DS. Eco-friendly stability-indicating HPTLC micro-determination of the first FDA approved SARS-CoV-2 antiviral prodrug Remdesivir: Study of degradation kinetics and structural elucidation of the degradants using HPTLC-MS. Sustain Chem Pharm. 2022;29(June):100744. doi:10.1016/j.scp.2022.100744
55.    Hussain MS, Fareed S, Ali M. Simultaneous HPTLC-UV530 nm analysis and validation of bioactive lupeol and stigmasterol in Hygrophila auriculata (K. Schum) Heine. Asian Pac J Trop Biomed. 2012;2(2, Supplement):S612-S617. doi:
56.    Raman S, Murugaiyah V, Parumasivam T. Andrographis paniculata Dosage Forms and Advances in Nanoparticulate Delivery Systems: An Overview. Molecules. 2022;27(19). doi:10.3390/molecules27196164
57.    Wall PE. Thin-Layer Chromatography RSC Chromatography Monographs.; 2005.
58.    Chen Q, Hou H, Zheng D, Xu X, Xi X, Chen Y. HPTLC screening of saccharin in beverages by densitometry quantification and SERS confirmation. RSC Adv. 2022;12(14):8317-8322. doi:10.1039/d1ra09416e
59.    Kumar S, Jyotirmayee K, Sarangi M. Thin layer chromatography: A tool of biotechnology for isolation of bioactive compounds from medicinal plants. Int J Pharm Sci Rev Res. 2013;18(1):126-132.
60.    Hussain S, Maqbool K, Naseer B. High Performance Thin Layer Chromatography: Principle, Working and Applications. Int J Pharm Pharm Sci. 2019;4(72):83-88.
61.    Ramu B, Chittela KB. High Performance Thin Layer Chromatography and its role in the Pharmaceutical Industry: Review. Sci J Biosci Bioeng. 2018;5(3):29-34.
62.    Rashmin P, Mrunali P, Nitin D, Nidhi D, Bharat P. HPTLC method development and validation: Strategy to minimize methodological failures. J Food Drug Anal. 2012;20(4). doi:10.6227/jfda.2012200408
63.    Wixom RL. Chromatography : A Science of Discovery. John Wiley and Sons; 2022.
64.    Gharge S, Koli R, Gudasi S, Hiremath SI. Spectrophotometric, chromatographic and bioanalysis of selected recently approved drugs from 2015–2020 to treat cardiovascular diseases: an analytical review. Bull Natl Res Cent. 2023;47(1). doi:10.1186/s42269-023-01000-5
65.    Chew YL, Khor MA, Lim YY. Choices of chromatographic methods as stability indicating assays for pharmaceutical products: A review. Heliyon. 2021;7(3):e06553. doi:10.1016/j.heliyon.2021.e06553
66.    Alam P, Shakeel F, Alqarni MH, et al. Development and Validation of A Stability-Indicating Greener HPTLC Method for the Estimation of Flufenamic Acid. Published online 2023:1-13.
67.    Stanek N, Jasicka-misiak I, Samples H. HPTLC Phenolic Profiles as Useful Tools for the Authentication of Honey Materials and Methods. 2018;(April):2979-2989.
68.    Chothani DL, Patel MB, Mishra SH.  HPTLC Fingerprint Profile and Isolation of Marker Compound of Ruellia tuberosa . Chromatogr Res Int. 2012;2012:1-6. doi:10.1155/2012/180103
69.    Ekar T, Kreft S. Common risks of adulterated and mislabeled herbal preparations. Food Chem Toxicol. 2019;123:288-297. doi:10.1016/j.fct.2018.10.043
70.    Momtaz M, Bubli SY, Khan MS. Mechanisms and Health Aspects of Food Adulteration: A Comprehensive Review. Foods. 2023;12(1). doi:10.3390/foods12010199
71.    Jităreanu A, Trifan A, Vieriu M, Caba IC, Mârțu I, Agoroaei L. Current Trends in Toxicity Assessment of Herbal Medicines: A Narrative Review. Processes. 2023;11(1). doi:10.3390/pr11010083
72.    Chan K. Some aspects of toxic contaminants in herbal medicines. Chemosphere. 2003;52(9):1361-1371. doi:10.1016/S0045-6535(03)00471-5
73.    Posadzki P, Watson L, Ernst E. Contamination and adulteration of herbal medicinal products (HMPs): An overview of systematic reviews. Eur J Clin Pharmacol. 2013;69(3):295-307. doi:10.1007/s00228-012-1353-z
74.    Kushwaha SKS, Kushwaha N, Maurya N, Rai  a K. Role of Markers in the Standardization of Herbal Drugs : A Review. Arch Appl Sci Res. 2010;2(1):225-229.
75.    Singh RS, Angra V, Singh A, Masih GD, Medhi B. Integrative omics - An arsenal for drug discovery. Indian J Pharmacol. 2022;54(1):1-6. doi:10.4103/ijp.ijp_53_22
76.    Singh R, Singh PK, Kumar R, et al. Multi-Omics Approach in the Identification of Potential Therapeutic Biomolecule for COVID-19. Front Pharmacol. 2021;12(May):1-17. doi:10.3389/fphar.2021.652335
77.    Sucher NJ, Carles MC. Genome-based approaches to the authentication of medicinal plants. Planta Med. 2008;74(6):603-623. doi:10.1055/s-2008-1074517
78.    Sheidai M, Tabaripour R, Talebi SM, Noormohammadi Z, Koohdar F. Adulteration in medicinally important plant species of Ziziphora in Iran market: DNA barcoding approach. Ind Crops Prod. 2019;130(January):627-633. doi:10.1016/j.indcrop.2019.01.025
79.    Shivatare R, Hanumansingh Nagore D. “HPTLC” an important tool in standardization of herbal medical product: A review Evaluation of anti-inflammatory and antimicrobial activity of AHPL/AYTOP/0213 cream View project HPTLC’ an important tool in standardization of herbal medical product: A revi. 2013;(August 2016).
80.    Spangenberg B, Seigel A, Brämer R. Screening of orange peel waste on valuable compounds by gradient multiple development diode-array high-performance thin-layer chromatography. J Planar Chromatogr - Mod TLC. 2022;35(3):313-330. doi:10.1007/s00764-022-00176-2
81.    Raghavan govindarajan and vyas Tejas PP. Tool for Standardization of Complex Herbal Drugs. Published online 2019:986-992.
82.    Gayakwad H. Technique Used in Detection of Herbal Drugs. 2023;14:1-5. doi:10.37421/2157-7145.2023.14.532
83.    Blum F. High performance liquid chromatography. Br J Hosp Med. 2014;75(SUPPL. 2). doi:10.12968/hmed.2014.75.Sup2.C18
84.    Parab Gaonkar V, Mannur VK, Hullatti K. Quality assessment and Analytical Quality by Design-based RP-HPLC method development for quantification of Piperine in Piper nigrum L. Futur J Pharm Sci. 2022;8(1):16. doi:10.1186/s43094-022-00405-9
85.    Park J, Kim K, Ryu D, Whang JH, Mah JH. GC-MS/MS Method for Determination of Polycyclic Aromatic Hydrocarbons in Herbal Medicines. Molecules. 2023;28(9). doi:10.3390/molecules28093853
86.    Kang SM, Won JH, Han JE, et al. Chromatographic Method for Monitoring of Pesticide Residues and Risk Assessment for Herbal Decoctions Used in Traditional Korean Medicine Clinics. Molecules. 2023;28(8). doi:10.3390/molecules28083343
87.    Zhou JL, Qi LW, Li P. Herbal medicine analysis by liquid chromatography/time-of-flight mass spectrometry. J Chromatogr A. 2009;1216(44):7582-7594. doi:10.1016/j.chroma.2009.05.054
88.    Noviana E, Indrayanto G, Rohman A. Advances in Fingerprint Analysis for Standardization and Quality Control of Herbal Medicines. Front Pharmacol. 2022;13(June):1-21. doi:10.3389/fphar.2022.853023
89.    Jin C, Sun D, Wei C, Guo Z, Yang C, Li F. Gas chromatography-mass spectrometry analysis of natural products in Gypsophila Paniculata. HortScience. 2021;56(10):1195-1198. doi:10.21273/HORTSCI16000-21
90.    Borisov R, Kanateva A, Zhilyaev D. Recent Advances in Combinations of TLC With MALDI and Other Desorption/Ionization Mass-Spectrometry Techniques. Front Chem. 2021;9(December):1-12. doi:10.3389/fchem.2021.771801
91.    Verma KL, Kumar M, Singh AP. HPTLC-MS as a Neoteric Hyphenated Technique for Separation and Forensic Identification of Drugs. J Anal Sci Methods Instrum. 2018;08(01):1-15. doi:10.4236/jasmi.2018.81001
92.    Sareen S, Joseph L, Mathew G. Improvement in solubility of poor water-soluble drugs by solid dispersion. Int J Pharm Investig. 2012;2(1):12. doi:10.4103/2230-973x.96921
93.    Coskun O. Separation Tecniques: CHROMATOGRAPHY. North Clin Istanbul. 2016;3(2):156-160. doi:10.14744/nci.2016.32757
94.    Teen Teh AH, Dykes GA. Meat Species Determination. Encycl Meat Sci. 2014;2:265-269. doi:10.1016/B978-0-12-384731-7.00209-9
95.    Wilson ID, Poole CF. Planar chromatography – Current practice and future prospects. J Chromatogr B Anal Technol Biomed Life Sci. 2023;1214(September 2022):123553. doi:10.1016/j.jchromb.2022.123553
96.    Rohman A, Indrayanto G, Ingkaninan K. Editorial: Development, assessment, improvement, and standardization of methods in herbal drug research. Front Pharmacol. 2022;13(November). doi:10.3389/fphar.2022.1071194
97.    Cieśla Ł, Hajnos M, Waksmundzka-Hajnos M. Application of hydrophilic interaction TLC systems for separation of highly polar glycosidic compounds from the flowers of selected Verbascum species. J Planar Chromatogr - Mod TLC. 2011;24(4):295-300. doi:10.1556/JPC.24.2011.4.4
98.    Abere TA, Okeri HA, Okafor LA. Comparative evaluation of selected starches as adsorbent for Thin-layer Chromatography. Trop J Pharm Res. 2007;4(1):331-339. doi:10.4314/tjpr.v4i1.14617
99.    A. Baerheim Svendsen - R. Verpoorte. Adsorbents, Solvent Systems and TLC Techniques. Vol Volume 23.; 1983.
100.    Esmaiili K, Shetab-Boushehri SV. Comparative verification study of silica gel-coated TLC and HPTLC plates’ performances in separation of opium alkaloids on the basis of their physicochemical properties. J Anal Sci Technol. 2017;8(1). doi:10.1186/s40543-017-0131-z
101.    Dar A, Ahmad MN, Samin G, et al. Separation of Amino Acids, Dyes, and Pigments Using Novel Pressurized Circular TLC Assembly for Secure Medical Imaging Applications. Int J Anal Chem. 2023;2023. doi:10.1155/2023/9914633
102.    Juszczak AM, Zovko-Končić M, Tomczyk M. Recent Trends in the Application of Chromatographic Techniques in the Analysis of Luteolin and Its Derivatives. Vol 9.; 2019. doi:10.3390/biom9110731
103.    Koll K, Reich E, Blatter A, Veit M. Validation of standardized high-performance thin-layer chromatographic methods for quality control and stability testing of herbals. J AOAC Int. 2003;86(5):909-915. doi:10.1093/jaoac/86.5.909
104.    Jayshree S. Gawali*1, S. S. Aher2 RBS. Asian Journal of Research in Chemistry and Pharmaceutical Sciences Analytical Method Development and Validation of Anticancer. Asian J Res Chem Pharm Sci. 2015;3(2):51-65.
105.    Choma I, Jesionek W. TLC-Direct Bioautography as a High Throughput Method for Detection of Antimicrobials in Plants. Chromatography. 2015;2(2):225-238. doi:10.3390/chromatography2020225
106.    Cahyono B, Setyadewi M. C, Suzery M, Aminin ALN. The Comparison of Spectrophotometric and TLC-Densitometric for DPPH Radical Scavenging Activity Analysis of Three Medicinal Plant Extracts. JKPK (Jurnal Kim dan Pendidik Kim. 2020;5(2):110. doi:10.20961/jkpk.v5i2.40370
107.    Matysik E, Woźniak A, Paduch R, Rejdak R, Polak B, Donica H. The New TLC Method for Separation and Determination of Multicomponent Mixtures of Plant Extracts. J Anal Methods Chem. 2016;2016. doi:10.1155/2016/1813581
108.    Sonia K, Shree BB, Lakshmi KS. HPTLC method development and validation: An overview. J Pharm Sci Res. 2017;9(5):652-657.
109.    Agrahari V, Bajpai M, Nanda S. Essential concepts of mobile phase selection for Reversed phase HPLC. Res J Pharm Technol. 2013;6(5):459-464.
110.    Patel RB, Patel MR, Bhatt KK, Patel BG.  Development and Validation of HPTLC Method for Estimation of Carbamazepine in Formulations and Its In Vitro Release Study . Chromatogr Res Int. 2011;2011:1-8. doi:10.4061/2011/684369
111.    Morlock G. New HPTLC method, with systematic mobile-phase optimization, for determination of six apolar heterocyclic aromatic amines. J Planar Chromatogr - Mod TLC. 2004;17(6):431-434. doi:10.1556/jpc.17.2004.6.6
112.    Hajmalek M, Goudarzi M, Ghaffari S, Attar H, Mazlaghan MG. Development and validation of a HPTLC method for analysis of sunitinib malate. Brazilian J Pharm Sci. 2016;52(4):595-602. doi:10.1590/S1984-82502016000400003
113.    Pandhare RB, Kulkarni RN, Deshmukh VK, Mohite PB, Pawar AR. High-performance thin layer chromatography: A powerful analytical technique in pharmaceutical drug discovery. J Pharm Biol Sci. 2021;9(1):7-14. doi:10.18231/j.jpbs.2021.002
114.    Tiwari S, Talreja S. Thin Layer Chromatography (TLC) VS. Paper Chromatography: A Review. Acta Sci Pharm Sci. 2022;6(9):5-9. doi:10.31080/ASPS.2022.06.0894
115.    Smith SERM. Thin-Layer Chromatography RSC Chromatography Monographs.
116.    Sutar PB, Shimpi VR, Kamble PU. A review on HPTLC method development and validation for lacosamide in tablet and bulk. 2022;9(5):578-597.
117.    Divya P, Sathishkumar M, Malik A, Jyothi N. A review on high performance thin layer chromatography. EPRA International Journal of Research and Development. (IJRD). 2021;7838(October):8-13.
118.    Suneela S. dhaneshwar Deshpande P, Patil M, Vadnerkar G, Dhaneshwar SR. Development and validation of a HPTLC method for Estimation of Duloxetine Hydrochloride in Bulk Drug and in Tablet Dosage Form. Indian J Pharm Sci. 2008;(March-Apri):233-236.
119.    Ravi TK. Simultaneous HPTLC Determination of Rabeprazole and Itopride Hydrochloride From Their Combined Dosage Form. 2008;(June).
120.    Analysis P, College RVS. HPTLC Method for the Simultaneous Estimation of Aceclofenac and Diacerein in Tablets Dosage Forms. 2010;3(3):825-827.
121.    Deepthi R. Instrumentation and future prospects of HPTLC- A REVIEW. 2021;10(6):650-661. doi:10.20959/wjpr20216-20560
122.    Ansari MJ, Ahmad S, Kohli K, Ali J, Khar RK. Stability-indicating HPTLC determination of curcumin in bulk drug and pharmaceutical formulations. J Pharm Biomed Anal. 2005;39(1-2):132-138. doi:10.1016/j.jpba.2005.03.021
123.    Chaudhary S, Deep Ankalgi A, Kaushal P, Singh Ashawat M. High Performance Thin Layer Chromatography With Mass Spectrometry: a Review. Int J Sci Dev Res. 2022;7(8):641-645.
124.    Agrawal H, Kaul N, Paradkar AR, Mahadik KR. Stability indicating HPTLC determination of clopidogrel bisulphate as bulk drug and in pharmaceutical dosage form. Talanta. 2003;61(5):581-589. doi:10.1016/S0039-9140(03)00364-3
125.    Parmar P, Mehta A. Development and Validation of HPTLC Method for the Estimation of Clotrimazole in Bulk drug and Tablet Formulation. Published online 2009:451-454.
126.    Wilson ID, Poole CF. Planar chromatography – Current practice and future prospects. J Chromatogr B Anal Technol Biomed Life Sci. 2023;1214(November 2022):123553. doi:10.1016/j.jchromb.2022.123553
127.    Patel Vishal R, Patel Rakesh K. HPTLC method Development and validation for quantification of markers of dhatrinisha churna. Pharmacogn J. 2012;4(29):26-29. doi:10.5530/pj.2012.29.4
128.    Sona D, Baghel GS, Chandravanshi L, et al. High-performance thin-layer chromatography-based quantification of therapeutic phytochemicals in the methanolic extract of Ayurvedic formulation Drakshavaleha. J Planar Chromatogr - Mod TLC. 2022;35(2). doi:10.1007/s00764-022-00173-5
129.    Patel S, Baghel GS, Chauhan NS, et al. High-performance thin-layer chromatographic standardization and quantification of marker compounds in an Ayurvedic polyherbal formulation: Krishnadi Churna. J Planar Chromatogr - Mod TLC. 2021;34(6). doi:10.1007/s00764-021-00144-2
130.    Foudah AI, Alam P, Anwer MK, Yusufoglu HS, Abdel-Kader MS, Shakeel F. A green RP-HPTLC-densitometry method for the determination of diosmin in pharmaceutical formulations. Processes. 2020;8(7). doi:10.3390/pr8070817
131.    Walode SG, Chaudhari HK, Saraswat MS, Kasture A V., Wadodkar SG. Validated high performance thin layer chromatographic determination and content uniformity test for rosiglitazone in tablets. Indian J Pharm Sci. 2010;72(2):249-252. doi:10.4103/0250-474X.65034
132.    Alam P, Haq N, Alqarni MH, Shakeel F. Quantitative Analysis of Emtricitabine in Dosage Forms Using Green RP-HPTLC and Routine NP-HPTLC Methods - A Contrast of Validation Parameters. ACS Omega. 2020;5(51):33470-33477. doi:10.1021/acsomega.0c05537
133.    Bonfilio R, Cazedey ECL, de Araújo MB, Salgado HRN. Analytical Validation of Quantitative High-Performance Liquid Chromatographic Methods in Pharmaceutical Analysis: A Practical Approach. Crit Rev Anal Chem. 2012;42(1):87-100. doi:10.1080/10408347.2012.630926
134.    Jenkins D, Diallo C, Bethea E, Kaale E, Layloff T. Method Validation Approaches for Pharmaceutical Assessments – Highlights with High Performance Thin Layer Chromatographic (HPTLC) Techniques. Calibration Valid Anal Methods - A Sampl Curr Approaches. Published online 2018. doi:10.5772/intechopen.71765
135.    Shaalan RA, Belal TS, El Yazbi FA, Elonsy SM. Validated HPTLC methods for determination of some selected antihypertensive mixtures in their combined dosage forms. Bull Fac Pharmacy, Cairo Univ. 2014;52(2):225-237. doi:10.1016/j.bfopcu.2014.07.001
136.    Delvadiya K, Kabra P, Kimbahune R, Patel N, Nargund LVG. High-performance liquid chromatographic determination of paracetamol, propyphenazone, and caffeine in pharmaceutical formulations. Indian J Pharm Educ Res. 2013;47(4):65-72. doi:10.5530/ijper.47.4.9
137.    Thomas A, Kanakdhar A, Shirsat A, Deshkar S, Kothapalli L. A high performance thin layer chromatographic method using a design of experiment approach for estimation of phytochemicals in extracts of Moringa oleifera leaves. Turkish J Pharm Sci. 2020;17(2):148-158. doi:10.4274/tjps.galenos.2018.80958
138.    Dołowy M, Pyka-Pająk A, Jampílek J. Simple and accurate HPTLC-densitometric method for assay of nandrolone decanoate in pharmaceutical formulation. Molecules. 2019;24(3). doi:10.3390/molecules24030435
139.    Shewiyo DH, Kaale E, Risha PG, et al. Accuracy profiles assessing the validity for routine use of high-performance thin-layer chromatographic assays for drug formulations. J Chromatogr A. 2013;1293:159-169. doi:10.1016/j.chroma.2013.03.074
140.    Ferenczi-Fodor K, Végh Z, Nagy-Turák A, Renger B, Zeller M. Validation and quality assurance of planar chromatographic procedures in pharmaceutical analysis. J AOAC Int. 2001;84(4):1265-1276. doi:10.1093/jaoac/84.4.1265
141.    Jadhav K, Dhamecha D, Tate A, Tambe H, Patil MB. Application of UV spectrophotometric method for easy and rapid estimation of lafutidine in bulk and pharmaceutical formulation. Pharm Methods. 2011;2(4):264-267. doi:10.4103/2229-4708.93398
142.    Shinde AC, Devhadrao N V, Bansode AS, Bansode AS. Analytical method development and validation of assay test of pravastatin sodium tablets. J Drug Deliv Ther. 2019;9(2):70-75. doi:10.22270/jddt.v9i2.2379
143.    Belouafa S, Habti F, Benhar S, et al. Statistical tools and approaches to validate analytical methods: Methodology and practical examples. Int J Metrol Qual Eng. 2017;8. doi:10.1051/ijmqe/2016030
144.    Bhole RP, Shinde SS, Chitlange SS, Wankhede B. A high-performance thin layer chromatography (HPTLC) method for simultaneous determination of diphenhydramine hydrochloride and naproxen sodium in tablets. Anal Chem Insights. 2015;10(1):47-51. doi:10.4137/Aci.s31506
145.    Dixit RP, Barhate CR, Nagarsenker MS. Stability-indicating HPTLC method for simultaneous determination of ezetimibe and simvastatin. Chromatographia. 2008;67(1-2):101-107. doi:10.1365/s10337-007-0459-4
146.    Shraddha S Dhumal DDPD and DSBN, Abstract. A research on development and validation of HPTLC method for simultaneous estimation of Ofloxacin, Clotrimazole and Ornidazole in their combined. J Pharmacogn Phytochem. 2019;8(4):1896-1907. doi:10.53879/id.51.04.p0020
147.    Genete G, Hymete A, Bekhit AA. Development and validation of HPTLC assay method for simultaneous quantification of hydrocortisone and clotrimazole in cream and applying for stability indicating test. J Chil Chem Soc. 2012;57(3):1199-1203. doi:10.4067/S0717-97072012000300001
148.    Kalshetti S, Rao KP. Development and validation of HPLC method for the quantification of clotrimazole in a gelatin film formulation. Futur J Pharm Sci. 2021;7(1). doi:10.1186/s43094-021-00291-7
149.    Hussain S, Munjewar RR, Farooqui M. Development and Validation of a Simultaneous HPLC Method for Quantification of Amlodipine Besylate and Metoprolol Tartrate in Tablets. 2012;1(2):1-5.
150.    Kirchert S, Morlock GE. Orthogonal Hyphenation of Planar and Liquid Chromatography for Mass Spectrometry of Biomarkers out of the Bioassay Matrix (NP-HPTLC-UV/vis/FLD-Bioassay-RP/IEX-HPLC-UV/vis-ESI-MS). Anal Chem. 2020;92(13):9057-9064. doi:10.1021/acs.analchem.0c01251
151.    Patel K, Patel J, Patel M, Rajput G, Patel H. Introduction to hyphenated techniques and their applications in pharmacy. Pharm Methods. 2010;1(1):2. doi:10.4103/2229-4708.72222
152.    Badyal PN, Sharma C, Kaur N, Shankar R, Pandey A, Rawal RK. Analytical Techniques in Simultaneous Estimation : An Overview. Austin J Anal Pharm Chem. 2015;2(2):1-14.
153.    Bhole RP, Jagtap SR, Chadar KB, Zambare YB. Review on hyphenation in HPTLC-MS. Res J Pharm Technol. 2020;13(2):1028-1034. doi:10.5958/0974-360X.2020.00189.4
154.    Agatonovic-Kustrin S, Morton DW. Hyphenated TLC as a tool in the effect-directed discovery of bioactive natural products. Appl Sci. 2020;10(3). doi:10.3390/app10031123
155.    Rachana R. Joshi* Krishna R. Gupta and Shitalkumar S. Patil Ashokrao. Hyphenated technique- A Boon to Analytical World. Int J Pharm Sci Res. 2012;3(11):4184-4191.
156.    Nagajyothi S, Swetha Y, Neeharika J, Suresh P V, Ramarao N. Hyphenated Techniques-A Comprehensive Review. Int J Adv Res Dev. 2017;2:63-71.
157.    Agatonovic-Kustrin S, Morton DW. The power of hptlc-atr-ftir hyphenation in bioactivity analysis of plant extracts. Appl Sci. 2020; 10(22):1-3. doi:10.3390/app10228232
158.    Jiang Y, Kang X, Gao D, et al. Finding a suitable separation condition for TLC/FTIR analysis by using multiple-narrow-band TLC technique. RSC Adv. 2015; 5(28): 21544-21549. doi:10.1039/c4ra13537g
159.    Andola HC. High Performance Thin Layer Chromatography (HPTLC): A Modern Analytical tool for Biological Analysis. 2010;8(10):58-61.

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

56th percentile
Powered by  Scopus

SCImago Journal & Country Rank

Recent Articles


Not Available