Author(s): Nataliya Stadnytska, Natalija Monka, Nazar Manko, Piotr Pavel Wieczorek, Iza Jasicka-Misiak, Tetiana Holubieva, Vira Lubenets


DOI: 10.52711/0974-360X.2023.00649   

Address: Nataliya Stadnytska, Natalija Monka, Nazar Manko, Piotr Pavel Wieczorek, Iza Jasicka-Misiak, Tetiana Holubieva, Vira Lubenets
3/4 St. Yura Square, Department of Technology of Biologically Active Substances, Pharmacy and Biotechnology, Lviv Ukraine, 79013.
*Corresponding Author

Published In:   Volume - 16,      Issue - 8,     Year - 2023

The increased demand for medicinal products of natural origin causes the search for new plants with a certain spectrum of pharmacological action. Scorzonera purpurea subsp. rosea is a one of unexplored plants in the phytochemical and pharmacological sense. The aim of this research was to evaluate the phenolic compounds, antioxidant activity and antibacterial effect of ethanolic extracts of Scorzonera purpurea subsp. rosea (EE-Sp). The results of the study of EE-Sp which contained 70% of ethanol (E70) showed the high content of phenolic compounds (2.111mg/mL), flavonoids (0.276mg/mL) and anti-radical activity of 86%, which was determined by the DPPH method, its effective concentration of inhibition IC50 - 0.67mg/mL as well as the strong ferric reducing potential (FRAP method) - 23.58µmol/mL.The HPLC method was used to identify chlorogenic, caffeic, ferulic, rosmarinic acids, luteolin, quercetin, apigenin, apigenin-7-glucoside, and rutin. Chlorogenic acid, luteolin and apigenin were the dominant substances in terms of quantity. Studies of the antibacterial effect of the extract E70 with respect to the test-strains of bacteria Staphylococcus aureus ATCC 25923, Escherichia coli dH5a, Pseudomonas aeruginosa ATCC 9027 showed the effectiveness of the extract compared to control.

Cite this article:
Nataliya Stadnytska, Natalija Monka, Nazar Manko, Piotr Pavel Wieczorek, Iza Jasicka-Misiak, Tetiana Holubieva, Vira Lubenets. The Study of the Phenolic compounds, Antioxidant activity and Antibacterial effect of Herbal Ethanol extracts of Scorzonera purpurea subsp. rosea. Research Journal of Pharmacy and Technology. 2023; 16(8):3945-0. doi: 10.52711/0974-360X.2023.00649

Nataliya Stadnytska, Natalija Monka, Nazar Manko, Piotr Pavel Wieczorek, Iza Jasicka-Misiak, Tetiana Holubieva, Vira Lubenets. The Study of the Phenolic compounds, Antioxidant activity and Antibacterial effect of Herbal Ethanol extracts of Scorzonera purpurea subsp. rosea. Research Journal of Pharmacy and Technology. 2023; 16(8):3945-0. doi: 10.52711/0974-360X.2023.00649   Available on:

1.    Tekeshwar K., Vishal J. Phytochemical screening, phenolic, flavonoids, carotenoids contents and antioxidant activity of folkloric Memecylon edule roxb. Research J. Pharm. and Tech. 2016; 9(10):1547-1551. doi: 10.5958/0974-360X.2016.00303.6
2.    Pavithra S., Banu N. Free radical scavenging activity and total antioxidant capacity of tin chlorophyllin from Morinda citrifolia L. Research J. Pharm. and Tech. 2017; 10(2): 453-455. doi: 10.5958/0974-360X.2017.00091.9
3.    Bindu J., Dheeraj A., Vibhor KJ., Bharati A. Comparison of phenolic content and antioxidant properties of aqueous ethanolic extracts of leaves of Mangifera indica L. and Nicotiana tabacum L. Research J. Pharm. and Tech. 2018; 11(2):717-722. doi: 10.5958/0974-360X.2018.00135.X
4.    Marref SE., Benkiki N., Melakhessou MA. In vitro antioxidant activity, total phenolics and flavonoids contents of Gladiolus segetum Extracts. Research J. Pharm. and Tech. 2018; 11(11): 5017-5023. doi: 10.5958/0974-360X.2018.00915.0
5.    Melakhessou MA., Benkiki N., Marref SE. determination of antioxidant capacity, flavonoids and total phenolic content of extracts from Atractylis flava desf. Research J. Pharm. and Tech. 2018; 11(12): 5221-5226. doi: 10.5958/0974-360X.2018.00952.6
6.    Shukla A., Kaur A., Shukla RK., Anchal. Comparative evaluation of antioxidant capacity, total flavonoid and phenolic content of Ehretia acuminata R. Br. fruit. Research J. Pharm. and Tech. 2019; 12(4):1811-1816. doi: 10.5958/0974-360X.2019.00302.0
7.    Fellah K., Amrouche A., Benmehdi H., Memmou F. Phenolic profile, Antioxidants and kinetic properties of flavonoids and tannins fractions isolated from Prunus persica L. Leaves growing in Southwest Algeria. Research J. Pharm. and Tech. 2019; 12(9):4365-4372. doi: 10.5958/0974-360X.2019.00751.0
8.    Som KM., Manik S., Javaid I., Mohd Y., Razda S. Phytochemical analysis, total flavonoid, phenolic contents and antioxidant activity of extracts from the leaves of Rhododendron arboreum. Research J. Pharm. and Tech. 2020; 13(4):1701-1706. doi: 10.5958/0974-360X.2020.00307.8
9.    Thilagavathi T., Kathiravan G. Phytochemical analysis and antimicrobial activity of ethonolic leaf extract of Ficus racemosa Linn. Research J. Pharm. and Tech. 2017; 10(2): 537-540. doi: 10.5958/0974-360X.2017.00107.X
10.    Kumar A., Mahajan A., Begum Z. Phytochemical screening and in vitro study of free radical scavenging activity of flavonoids of Aloe vera. Research J. Pharm. and Tech. 2020; 13(2):593-598. doi: 10.5958/0974-360X.2020.00112.2
11.    Bargah RK., Kushwaha A., Tirkey A., Hariwanshi B. In Vitro antioxidant and antibacterial screening of flowers extract from Cassia auriculata Linn. Research J. Pharm. and Tech. 2020; 13(6):2624-2628. doi: 10.5958/0974-360X.2020.00466.7
12.    Sopan N. Kharat, Nida Ansari, Vijay D. Mendhulkar. HPTLC screening for flavonoids content in leaf extracts of Syzygium cumini (Linn.) and its Antimicrobial Activity. Research J. Pharm. and Tech. 2020; 13(6): 2720-2726. doi: 10.5958/0974-360X.2020.00484.9
13.    Pavlyuk I, Stadnytska N, Jasicka-Misiak I, Gorka B, Wieczorek PP, Novikov V. A study of the chemical composition and biological activity of extracts from wild carrot (Daucus carota L.) seeds waste. Research Journal of Pharmaceutical, Biological and Chemical Sciences. 2015; 6(2): 603-11.
14.    Pavliuk IV., Stadnytska NYe., Yasitska-Misiak I., Wechorek P., Zahorii HV., Brezvyn OM., Rudyk HV, Novikov VP. Doslidzhennia biolohichnoi aktyvnosti vtorynnoho ekstraktu zi shrotu travy materynky zvychainoi (Origanum vulgare). Ukrainskyi Biofarmatsev Tychnyi Zhurnal. 2015; 36: 21-4.
15.    Pavliuk I., Stadnytska N., Novikov V. Investigation of the kinetics of extraction of flavonoids from hop cones meal. Eastern-European Journal of Enterprise Technologies, 2015; 5/11(77): 36-41. doi: 10.15587/1729-4061.2015.50965
16.    Akkol EK., Acıkara OB., Süntar I., Citoglu GS., Keles H., Ergene B. Enhancement of wound healing by topical application of Scorzonera species: Determination of the constituents by HPLC with new validated reverse phase method. Journal of Ethnopharmacology. 2011; 137: 1018-27. doi: 10.1016/j.jep.2011.07.029
17.    Bader A, Tommasi ND, Cotugno R, Braca A. Phenolic compounds from the roots of Jordanian Viper’s Grass, Scorzonera judaica. Journal of Natural Products. 2011; 74: 1421-6. doi: 10.1021/np200143s
18.    Ugur A., Sarac N., Ceylan O., Duru ME., Beyatli Y. Chemical composition of endemic Scorzonera sandrasica and studies on the antimicrobial activity against multiresistant bacteria. Journal of Medicinal Food. 2010; 13(3): 635-9. doi: 10.1089/jmf.2008.0312
19.    Xie Y., Guo Q-S., Wang G-S. Preparative Separation and Purification of the Total Flavonoids in Scorzonera austriaca with Macroporous resins. Molecules. 2016; 21(768):1-11. doi: 10.3390/molecules21060768
20.    Abdelkader HB, Salah KBH, Liouane K, Boussaada O, Gafsi K, Mahjoub MA et al. Antimicrobial activity of Rhaponticum acaule and Scorzonera undulata growing wild in Tunisia. African Journal of Microbiology Research. 2010; 4: 1954–8.
21.    Harkati B, Akkal S, Bayat C, Laouer H, Franc, MGD. Secondary metabolites from Scorzonera undulata ssp. Deliciosa (Guss.) Maine (Asteraceae) and their antioxidant activities. Records of Natural Products. 2010; 4: 171–5.
22.    Keser S, Kak O. In vitro antimicrobial, antiradical, anticancer evaluation, and phytochemical contents of endemic Scorzonera semicana DC. Journal of Food Processing and Preservation. 2021; 45: 15971. doi: 10.1111/jfpp.15971
23.    Nesteruk Y. The plant world of the Ukrainian Carpathians: Chornohora: ecological travels: Reference book. Ukraine:Lviv; 2003.
24.    Lucian D, Cosmin CI. A short description of Scorzonera plant species present in Alexandru Beldie herbarium from I.N.C.D.S. Bucharest. Analele Universităţii din Craiova, seria Agricultură – Montanologie – Cadastru (Annals of the University of Craiova - Agriculture, Montanology, Cadastre Series. 2017; XLVII: 118-26.
25.    Yang YJ, Liu X, Wu HR, He XF, Bi YR, Zhu Y et al. Radical scavenging activity and cytotoxicity of active quinic acid derivatives from Scorzonera divaricata roots. Food Chemistry. 2013; 138: 2057-63. doi: 10.1016/j.foodchem.2012.10.122
26.    Wu Q-X, Su Y-B, Zhu Y. Triterpenes and steroids from the roots of Scorzonera austriaca. Fitoterapia. 2011; 82: 493-6. . doi: 10.1016/j.fitote.2011.01.006.
27.    Akkol EK, Smejkal K, Kurtul I, Ilhan M, Guragac FT, Iscan GS et al. Inhibitory activity of Scorzonera latifolia and its components on enzymes connected with healing process. Journal of Ethnopharmacology. 2019; 245: 112168:1-7. doi: 10.1016/j.jep.2012.01.015
28.    Acıkara OB, Ozbilgin S, Saltan-̇Iscan G, Dall’Acqua S, Rjaskova V, Ozgokce F et al. Phytochemical Analysis of Podospermum and Scorzonera n-Hexane Extracts and the HPLC Quantitation of Triterpenes. Molecules. 2018; 23: 1813:1-12. doi: 10.3390/molecules23071813
29.    Sahina H, Sarı A, Ozsoy N, Çelik BO, Koyuncu O. Two new phenolic compounds and some biological activities of Scorzonera pygmaea Sibth. & Sm. subaerial parts. Natural Product Research. 2018; 34: 621-8. doi: 10.1080/14786419.2018.1493585
30.    Granica S, Lohwasser U, Johrer K, Zidorn C. Qualitative and quantitative analyses of secondary metabolites in aerial and subaerial of Scorzonera hispanica L. (black salsify). Food Chemistry. 2015; 173: 321-31. doi: 10.1016/j.foodchem.2014.10.006
31.    Acikara OB, Hosek J, Babula P, Cvacka J, Budešínsky M, Dracinsky M et al. Turkish Scorzonera species extracts attenuate cytokine secretion via inhibition of NF-κB activation, showing anti-inflammatory effect in vitro. Molecules. 2016; 21(43): 1-14. doi: 10.3390/molecules21010043
32.    Akkol EK, Acıkara OB, Süntar I, Ergene B, Çitoglu GS. Ethnopharmacological evaluation of some Scorzonera species: In vivo anti-inflammatory and antinociceptive effects. Journal of Ethnopharmacology. 2012; 140: 261-70. doi: 10.1016/j.jep.2012.01.015
33.    Wu QX, He XF, Jiang CX, Zhang W, Shi Z-N, Li HF et al. Two novel bioactive sulfated guaiane sesquiterpenoid salt alkaloids from theaerial parts of Scorzonera divaricata. Fitoterapia. 2018; 124: 113-19. doi: 10.1016/j.fitote.2017.10.011
34.    Bahadır O, Citoglu GS, Smejkal K, Dall’Acqua S, Ozbek H, Cvacka J et al. Analgesic compounds from Scorzonera latifolia (Fisch. and Mey.) DC. Journal of Ethnopharmacology. 2010; 131: 83-7. DOI: 10.1016/j.jep.2010.06.003
35.    Sweidan A, El-Mestrah M, Kanaan H, Dandache I. Antibacterial and antibiofilm activities of Scorzonera mackmeliana. Pak. J. Pharm. Sci. 2020; 33(1): 199-206.
36.    Athmouni K, Belghith T, Bellassouad K, Ayadi AH. Effect of extraction solvents on the biomolecules and antioxidant properties of Scorsonera undulata (Asteraceae): application of factorial design optimisation phenolic extraction. Acta Sci. Pol. Technol. Aliment. 2015; 14(4): 313-30. doi: 10.17306/J.AFS.2015.4.32
37.    Dobrochaeva D.N., Kotov M.I., Prokudin YU.M. i dr. Opredelitel' vysshih rastenij Ukrainy. Kyiv: Fіtosocіocentr; 1999.
38.    Liubas N, Iskra R, Stadnytska N, Monka N, Havryliak V, Lubenets V. Antioxidant activity of thiosulfonate compounds in experiments in vitro and in vivo. Biointerface Research in Applied Chemistry. 2022; 12(3): 3106–16. Doi: 10.33263/BRIAC123.31063116
39.    Lootsik M, Manko N, Gromyko O, Tistechok S, Lutsyk M, Stoika R. Honeybee chitosan-melanin complex: isolation and investigation of antimicrobial activity. Ukrainian Biochemical Journal. 2020; 92(6): 143-53 doi: 10.15407/ubj92.06.143

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