Author(s): Yazan Islim, Dima Muhammad


DOI: 10.52711/0974-360X.2023.00648   

Address: Yazan Islim1*, Dima Muhammad1,2
1Undergraduate Student, Faculty of Pharmacy, Manara University, Latakia, Syria.
2PhD at the Department of Pharmacognosy at the Faculty of Pharmacy, Tishreen University, Latakia, Syria.
*Corresponding Author

Published In:   Volume - 16,      Issue - 8,     Year - 2023

This study aims to investigate the antibacterial activity of the ethanolic extract of common myrtle “Myrtus communis Linn.” leaves (Myrtaceae), specifically against Escherichia coli and Staphylococcus aureus isolated from patients at Tishreen University Hospital, Syria. Leaves were subjected to ultrasound-assisted extraction using the distilled-ethanol as a solvent. The dried extract, with a yield of 25.3%, was dissolved in two types of solvent Dimethylsolfoxide (DMSO), and a mixture of Tween 80 solution 10% and DMSO 8:2 and then tested for antibacterial activity using the standard disk diffusion technique. In this work, DMSO showed better properties in the improvement of the solubility and the diffusion of the prepared extract, thus enhancing its antibacterial action.The inhibition zones showed that the tested ethanolic extract has interesting activity against both tested strains with higher activity towards S. aureus.The overall results encourage the application of this extract to combat bacterial resistance, and as an additive in the food industry to control the foodborne disease caused by S. aureus.

Cite this article:
Yazan Islim, Dima Muhammad. Antibacterial study of the Ethanolic extract of local common Myrtle leaves Myrtus communis L. (Myrtaceae). Research Journal of Pharmacy and Technology. 2023; 16(8):3941-4. doi: 10.52711/0974-360X.2023.00648

Yazan Islim, Dima Muhammad. Antibacterial study of the Ethanolic extract of local common Myrtle leaves Myrtus communis L. (Myrtaceae). Research Journal of Pharmacy and Technology. 2023; 16(8):3941-4. doi: 10.52711/0974-360X.2023.00648   Available on:

1.    Rohit Raj, Chandrashekar. K. S, Vasudev Pai. Antimicrobial activity of Syzygium caryophyllatum L. Research Journal of Pharmacy and Technology. 2021; 14(8):4275-6. doi: 10.52711/0974-360X.2021.00742
2.    Nikhat F, D Satynarayanaa, Arun B Joshia. Phytochemical and Pharmacological Investigation of Roots of Syzygium Cuminii (L) Skeel. Asian J. Research Chem. 2008; 1(1): 22-25.
3.    Shilpa Maria, IJ Kuppast, JH Virupaksha, M Vanajakshi, K Prasad. Glossary of Callistemon lanceolatus D.C. – An ornamental plant with Marvellous Properties. Research Journal of Pharmacology and Pharmacodynamics. 2015; 7(4): 165-170 DOI 10.5958/2321-5836.2015.00033.6
4.    Males Z, et al. Comparative phytochemical and antimicrobial investigations of Hypericum perforatum L. subsp. perforatum and H. perforatum subsp. angustifolium (DC.) Gaudin. Acta Pharm. 2006; 56: 359–367 PMID: 19831284.
5.    Nassar MI, et al. Secondary Metabolites and Bioactivities of Myrtus communis. Pharmacognosy Res. 2014; 2: doi: 10.4103/0974-8490.75449
6.    Sabiha Sumbul, et al. Myrtus communis Linn. - A review, Indian Journal of Natural Products and Resources. 2011; 2(4): 395-402
7.    Charles DJ, Myrtle in Antioxidant properties of spices, herbs, and other sources, New York, 2013
8.    Ghazal Alipour Talesh, Saeedeh Dashti and Hossein Hosseinzadeh, Review of Pharmacological Effects of Myrtus communis L. and its Active Constituents, Phytotherapy Research, 2014, DOI: 10.1002/ptr.5122
9.    Aishwarya J. Ramalingam. History of Antibiotics and Evolution of Resistance. Research J. Pharm. and Tech. 2015; 8(12): 1719-1724. DOI 10.5958/0974-360X.2015.00309.1
10.    Hisham A. Abbas, Mona A. El-Saysed, Amira M. Ganiny, Azza Abdel Fattah. Antimicrobial Resistance Patterns of Proteus mirabilis isolates from Urinary tract, burn wound and Diabetic Foot Infections. Research J. Pharm. and Tech. 2018; 11(1): 249-252. DOI 10.5958/0974-360X.2018. 00046.X
11.    Sreeja. M.K, Gowrishankar N.L, Adisha. S, Divya. K.C. Antibiotic Resistance-Reasons and the Most Common Resistant Pathogens – A Review. Research J. Pharm. and Tech. 2017; 10(6): 1886-1890. DOI 10.5958/0974-360X.2017.00331.6
12.    Wilma Mary Thomson, M. Sudha, N. Venkateswaramurthy, R. Sambath Kumar. A Review on the Irrational Antibiotics usage in Pediatrics for Respiratory Tract Infections. Research J. Pharm. and Tech. 2019; 12(10):5126-5130. DOI 10.5958/0974-360X.2019.00888.6
13.    Mushtaq A. Mir, et al., Oteef, GC-MS analysis of Myrtus communis extract and its antibacterial activity against Gram- positive bacteria, 2020, doi: 10.1186/s12906-020-2863-3
14.    Dima Muhammad, et al., Triterpenoids from the leaves of Alphitonia xerocarpus Baill and their biological activity. Phytochemistry. 2016; 129 DOI: 10.1016/j.phytochem.2016.07.005
15.    Cockerill Franklin R., et al., Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard-ninth Edition. CLSI Clinical and Laboratory Standards Institute ISBN978-1-56238-784-6, p52
16.    Claudia Giuliani, et al., Botanic Garden as a Factory of Molecules: Myrtus communis L. subsp. communis as a Case Study. Plants 2022; 11: 754. DOI: 10.3390/plants11060754
17.    Rachna Chaturvedi, Preeti Chandra, Vineeta Mittal. Biofilm Formation by Acinetobacter Spp. in association with Antibiotic Resistance in clinical samples Obtained from Tertiary Care Hospital. Research J. Pharm. and Tech. 2019; 12(8): 3737-3742. DOI 10.5958/0974-360X.2019.00620.6
18.    M. Deepigaa, Gopinath P. Detection of Vancomycin Resistance among Clinical Isolates of Staphylococcus aureus. Research J. Pharm. and Tech. 2018; 11(2):621-623. DOI 10.5958/0974-360X.2018.00115.4
19.    Kishnani Khushboo, Bhandari Saloni, Rathore Kamal Singh. A Briefing of a Global Crisis: Antibiotic Resistance. Asian J. Res. Pharm. Sci. 2020; 10(4): 264-272. DOI 10.5958/2231-5659.2020.00047.8
20.    Nielsen CK, et al. Effects of Tween 80 on Growth and Biofilm Formation in Laboratory Media. Front. Microbiol. 2016; 7:1878. doi: 10.3389/fmicb.2016.01878

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

56th percentile
Powered by  Scopus

SCImago Journal & Country Rank

Recent Articles


Not Available