Author(s): Pandian PaneerSelvam, Usha Kumari, Dharma Raj Tanimale, Mohamed Asem, Sherly Deborah George, Subramani Parasuraman


DOI: 10.52711/0974-360X.2023.00619   

Address: Pandian PaneerSelvam1,2, Usha Kumari1, Dharma Raj Tanimale1,2, Mohamed Asem1,2, Sherly Deborah George3, Subramani Parasuraman4
1Unit of Physiology, Faculty of Medicine, AIMST University, Kedah, Malaysia.
2Clinical Skills Centre, Faculty of Medicine, AIMST University, Kedah, Malaysia.
3Department of Physiology, Faculty of Medicine, Manipal University College Malaysia, Melaka, Malaysia.
4Department of Pharmacology, Faculty of Pharmacy, AIMST University, Kedah, Malaysia.
*Corresponding Author

Published In:   Volume - 16,      Issue - 8,     Year - 2023

Background: Hesperidin is a naturally occurring Bioflavonoid found in citrus fruits. It has anti-inflammatory, antioxidant, antihypertensive, antimicrobial, anti-carcinogenic and vasodilator activities. Hesperidin’s effect on normal behavioral is not clear. Hence the present study is aimed to elucidate the effects of hesperidin on behavioral and biochemical markers of liver and kidney function in Sprague Dawley (SD) rats. Method: Healthy, adult, male SD rats were used for the study. Animals were divided into 5 groups (n = 6) viz., Group I: Control, Group II: Vitamin C(200mg/kg), Group III: Hesperidin (25mg/kg), Group IV: Hesperidin (50mg/kg) and GroupV: Hesperidin (100mg/kg). The standard and test drugs were suspended in 0.5% w/v carboxymethyl cellulose and administered once daily through oral gavage for 28 consecutive days. Throughout the study changes in behavioral functions (locomotor activity, muscular strength, learning and memory) and body weight were monitored at regular intervals. Blood samples were collected from all the experimental rats and used for biochemical parameters analysis. Results: Vitamin C and hesperidin did not show any significant alterations in locomotion, grip strength, muscular strength and spatial memory when compared with normal control. Both vitamin C and hesperidin improved the normal anxiety behavior without affecting regular body weight gain and biochemical markers of liver and kidney function. Conclusion: The experimental rats administered with hesperidin at the dose levels of 25, 50and100 mg/kg did not show any changes in psychomotor behavior and significantly improved their alertness without affecting the biochemical markers of liver and kidney function.

Cite this article:
Pandian PaneerSelvam, Usha Kumari, Dharma Raj Tanimale, Mohamed Asem, Sherly Deborah George, Subramani Parasuraman. Elucidating the Effect of hesperidin on Behavioral and biochemical markers of liver and kidney function in Sprague Dawley Rats. Research Journal of Pharmacy and Technology 2023; 16(8):3749-4. doi: 10.52711/0974-360X.2023.00619

Pandian PaneerSelvam, Usha Kumari, Dharma Raj Tanimale, Mohamed Asem, Sherly Deborah George, Subramani Parasuraman. Elucidating the Effect of hesperidin on Behavioral and biochemical markers of liver and kidney function in Sprague Dawley Rats. Research Journal of Pharmacy and Technology 2023; 16(8):3749-4. doi: 10.52711/0974-360X.2023.00619   Available on:

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