The present work is performed on analytical method development with its latter validation study of a simple and accurate UV-Visible spectrophotometric method for the content determination of the Pazopanib HCl (PAZ). The optimum conditions and solvent selection for the analysis of the drug were established. Absorption maxima (?max) taken for the analysis was 267nm in the purposed medium. Calibration curve was obtained and plotted against the observed values. Validation of the method was performed as per the current ICH guidelines for linearity, accuracy, precision, LOD and LOQ. The method is simple, accurate, sensitive, precise and reproducible. The method can be used in IPQC and QC testing on a commercial stage as well as in small scale labs, as the method is economical and cheap. The analysis is done on UV-Visible spectrophotometer which is easily available in every institute or in small labs as well.
Cite this article:
Ramendu Mishra, Anjana Devi. Spectrophotometric method development and validation for determination of pazopanib in the bulk and the formulation. Research Journal of Pharmacy and Technology 2023; 16(8):3633-7. doi: 10.52711/0974-360X.2023.00598
Ramendu Mishra, Anjana Devi. Spectrophotometric method development and validation for determination of pazopanib in the bulk and the formulation. Research Journal of Pharmacy and Technology 2023; 16(8):3633-7. doi: 10.52711/0974-360X.2023.00598 Available on: https://rjptonline.org/AbstractView.aspx?PID=2023-16-8-18
1. Miyamoto S, Kakutani S, Sato Y, Hanashi A, Kinoshita Y, Ishikawa A. Drug review: Pazopanib. Jpn J Clin Oncol. 2018;48(6):503–13.
2. Perazella MA, Dreicer R, Rosner MH. Renal cell carcinoma for the nephrologist. Kidney Int [Internet]. 2018;94(3):471–83. Available from: https://doi.org/10.1016/j.kint.2018.01.023
3. Escudier B, Porta C, Schmidinger M, Rioux-Leclercq N, Bex A, Khoo V, et al. Renal cell carcinoma: ESMO Clinical Practice Guidelines for diagnosis, treatment and follow-up. Ann Oncol. 2019; 30(5):706–20.
4. Verheijen RB, Beijnen JH, Schellens JHM, Huitema ADR, Steeghs N. Clinical Pharmacokinetics and Pharmacodynamics of Pazopanib: Towards Optimized Dosing. Clin Pharmacokinet. 2017; 56(9):987–97.
5. Hara N. Uv-Visible Spectroscopy. Zairyo-to-Kankyo. 1993; 42(8): 529–38.
6. Kothekar V. Basic UV-Vis Theory, Concepts and Applications Basic UV-Vis Theory, Concepts and Applications. Protocol. 2012;1–28.
7. Owen T. Principles and Applications of Timing Spectroscopy. Hewlett-Packard. 1996;24.
8. Pokala RV, Kumari K, Bollikola HB. Uv Spectrophotometry Method Development and Validation of Sulfadiazine and Trimethoprim in Combined Dosage Form. Int J Pharm Pharm Sci. 2018; 10(1): 103.
9. Agency EM. European Medicines Agency: An unacceptable choice. Prescrire Int. 2011;20(121):278.
10. da Silva Guedes J, da Silva Guedes ML. Quantificação do indicador de Nelson de Moraes (curva de mortalidade proporcional). Rev Saude Publica. 2006; 40(6): 951–61.
11. BR J, KV S, PP K. Development and Validation of UV Spectroscopic First Derivative Method for Simultaneous Estimation of Dapagliflozin and Metformin Hydrochloride in Synthetic Mixture. J Bioequivalence Stud. 2015;(July).
12. Khanage SG, Mohite PB, Jadhav S. Development and validation of UV-visible spectrophotometric method for simultaneous determination of eperisone and paracetamol in solid dosage form. Adv Pharm Bull. 2013;3(2):447–51.
13. Pankti D, Kusum M, Mehul P. Development and validation of UV-Visible Spectrophotometric method for simultaneous estimation of momentasone furoate, hydroquinone and tretinoin from their pharmaceutical dosage form. Int J Pharm Sci Rev Res. 2013; 21(1): 296–300.
14. Khanchandani SS, Galgatte UC, Chaudhari PD. Development and validation of UV-visible spectroscopic method for estimation of rizatriptan benzoate in bulk and tablet dosage form. Asian J Pharm Clin Res. 2013; 6(4): 113–6.
15. Jain PS, Chaudhari AJ, Patel SA, Patel ZN, Patel DT. Development and validation of the UV-spectrophotometric method for determination of terbinafine hydrochloride in bulk and in formulation. Pharm Methods. 2011; 2(3): 198–202.