Author(s):
Fina Syifa’una Musthoza, Raden Joko Kuncoroningrat Susilo, Suhailah Hayaza, Qurrotu A’ yun, Nur’aini Fikriyah, Ufairanisa Islamatasya, Dwi Winarni, Sri Puji Astuti Wahyuningsih, Ruey-an Doong, Deya Karsari, Aristika Dinar Yanti, Aulia Umi Rohmatika, Win Darmanto
Email(s):
windarmanto@fst.unair.ac.id
DOI:
10.52711/0974-360X.2023.00590 
Address:
Fina Syifa’una Musthoza1, Raden Joko Kuncoroningrat Susilo2, Suhailah Hayaza2, Qurrotu A’ yun1, Nur’aini Fikriyah1, Ufairanisa Islamatasya1, Dwi Winarni3, Sri Puji Astuti Wahyuningsih3, Ruey-an Doong4, Deya Karsari5, Aristika Dinar Yanti5, Aulia Umi Rohmatika1, Win Darmanto3,6*
1Magister Program in Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya, 60115, Indonesia.
2Department of Nanotechnology Engineering, Faculty of Advance Technology and Multidiscipline, Universitas Airlangga, Surabaya, 60115, Indonesia.
3Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya-60115, Indonesia.
4Institute of Analytical and Environmental Sciences, National Tsing Hua University, Sec. 2 Kuang Fu Road, Hsinchu 30013, Taiwan.
5Stem Cell Research and Development Center, Universitas Airlangga, Surabaya, 60115, Indonesia.
6Institute of Science Technology and Health, Jombang 61419, Indonesia.
*Corresponding Author
Published In:
Volume - 16,
Issue - 8,
Year - 2023
ABSTRACT:
Polysaccharide extract has potential as an anticancer agent. Abelmoschus esculentus is one of the plants that is rich in polysaccharide content, but its anticancer activity is still need explore. This study aimed to explore the anticancer activity of Abelmoschus esculentus crude extract polysaccharide by measure the effect on levels of pro-inflammatory cytokines IL-2, IL-12, TNF-a, IFN-? in HeLa cell line. This study used HeLa cells as a research model with four groups, including KN (media and DMSO), K+ (doxorobicin µg/mL), P1 (Abelmoschus esculentus L. extract 200µg/mL) and P2 (Abelmoschus esculentus L. extract 400µg/mL). The Abelmoschus esculentus L. was extracted using boiled distilled water. Cytokine levels were analyzed using the ELISA test. The results of cytokine levels showed decreasing in the levels of cytokines IL-2, IL-12, TNF-a, IFN-?, (P < 0.005). In conclusion, the polysaccharide extract of Abelmoschus esculentus L. has the potential as an anticancer and therapeutic agent for cancer. Research on green okra polysaccharide crude extract as an immunosuppressant is expected to be a potential bio-pharmaceutical source.
Cite this article:
Fina Syifa’una Musthoza, Raden Joko Kuncoroningrat Susilo, Suhailah Hayaza, Qurrotu A’ yun, Nur’aini Fikriyah, Ufairanisa Islamatasya, Dwi Winarni, Sri Puji Astuti Wahyuningsih, Ruey-an Doong, Deya Karsari, Aristika Dinar Yanti, Aulia Umi Rohmatika, Win Darmanto. In Vitro Analysis of Anticancer Potential of Green Okra (Abelmoschus esculentus L.) Polysaccharide Extracts in HeLa Cervical Cancer Cell Line. Research Journal of Pharmacy and Technology 2023; 16(8):3578-2. doi: 10.52711/0974-360X.2023.00590
Cite(Electronic):
Fina Syifa’una Musthoza, Raden Joko Kuncoroningrat Susilo, Suhailah Hayaza, Qurrotu A’ yun, Nur’aini Fikriyah, Ufairanisa Islamatasya, Dwi Winarni, Sri Puji Astuti Wahyuningsih, Ruey-an Doong, Deya Karsari, Aristika Dinar Yanti, Aulia Umi Rohmatika, Win Darmanto. In Vitro Analysis of Anticancer Potential of Green Okra (Abelmoschus esculentus L.) Polysaccharide Extracts in HeLa Cervical Cancer Cell Line. Research Journal of Pharmacy and Technology 2023; 16(8):3578-2. doi: 10.52711/0974-360X.2023.00590 Available on: https://rjptonline.org/AbstractView.aspx?PID=2023-16-8-10
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