Author(s):
Rabab Kamel, Nahla A. El-Wakil, Nermeen A. Elkasabgy
Email(s):
nermeen.ahmed.elkasabgy@pharma.cu.edu.eg
DOI:
10.52711/0974-360X.2023.00569 
Address:
Rabab Kamel1, Nahla A. El-Wakil2, Nermeen A. Elkasabgy3*
1Pharmaceutical Technology Department, National Research Centre, Cairo, Egypt.
2Cellulose and Paper Department, National Research Centre, Cairo, Egypt.
3Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, Cairo, 11562 Egypt.
*Corresponding Author
Published In:
Volume - 16,
Issue - 7,
Year - 2023
ABSTRACT:
Injectable in-situ forming hydrogel scaffolds (IHS) were prepared using TEMPO-oxidized nanofibrillated cellulose (TONFC) originating from sugarcane bagasse. TONFC (0.5%w/w) was prepared and characterized, then various concentrations of poloxamer 407 (P407) were added to prepare the thermo-responsive hydrogels. Two sources of calcium; Fujicalin® (DCP) or Hydroxyapatite (TCP), were utilized to prepare the calcium-enriched HIS loaded with the antiresorptive drug raloxifene hydrochloride. Physicochemical evaluation comprising the gelation temperature, drug content, injectability and in-vitro drug release were performed in addition to the examination of the morphological characters. The chosen formulation 'Ca-IHS4' consisted of TONFC, 15% P407 and 10% TCP displayed the most extended release pattern (for 12 days) with the smallest burst effect. SEM images of the in-situ formed scaffolds presented a highly porous 3D structure essential for cells migration, nutrient transport and tissue infiltration. Cell biology studies were performed using Saos-2 cells and proved that “Ca-IHS4” is biocompatible and has cell regenerative effect. These findings suggest that Ca-IHS4 presents a simple, safe and non-invasive platform for bone regeneration.
Cite this article:
Rabab Kamel, Nahla A. El-Wakil, Nermeen A. Elkasabgy. Injectable hydrogel scaffolds composed of Nanocellulose derived from sugarcane bagasse and combined with calcium for Bone regeneration. Research Journal of Pharmacy and Technology 2023; 16(7):3439-0. doi: 10.52711/0974-360X.2023.00569
Cite(Electronic):
Rabab Kamel, Nahla A. El-Wakil, Nermeen A. Elkasabgy. Injectable hydrogel scaffolds composed of Nanocellulose derived from sugarcane bagasse and combined with calcium for Bone regeneration. Research Journal of Pharmacy and Technology 2023; 16(7):3439-0. doi: 10.52711/0974-360X.2023.00569 Available on: https://rjptonline.org/AbstractView.aspx?PID=2023-16-7-66
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