ABSTRACT:
Object Moringa oleifera has numerous medicinal values and uses worldwide. Moringa oleifera is applied to remedy numerous diseases like malaria, arthritis, parasitic diseases, typhoid fever, swelling, cuts, and diseases of the skin, diabetes, and hypertension. However, no scientific evidence is available about the cardio-tropic activity of Moringa oleifera. The current research was carried out to investigate the cardio-tropic activity of hydro-alcoholic extract of Moringa oleifera leaves on the isolated heart of the Albino Wistar rat. The Moringa oleifera leaves were collected, washed, shade dried, and extracted with 70% ethanol. A stock solution (100µg/ml) was prepared by using distilled water. The cardio-tropic activity was screened on the isolated rat heart at a dose of 0.1ml, 0.2ml, and 0.4ml of 70% ethanolic extract of the Moringa oleifera leaf. Acetylcholine (0.0125ml, 0.0250ml, 0.0500ml, 0.1000ml) and adrenaline (0.05ml, 0.1ml, 0.2ml, 0.4ml) were used as standard drugs. Also, on the isolated rat heart, the dose-response curve of 70% ethanolic extract of the Moringa oleifera leaf and the standard drugs were screened at the above same dose. The 70% ethanolic extract of Moringa oleifera leaf (100µg/ml) has produced significant negative cardio-tropic activity as compared to standard drugs. The study indicates that 70% ethanolic extract of Moringa oleifera leaf shows substantial negative cardio-tropic activity on the isolated rat heart in dose dependent manner.
Cite this article:
Hira Das, Mrityunjoy Majumdar, Sourav Roy, Prodip Roy, Shristi Singh, Sohel Mondal, Asmita Das. Preclinical Evaluation of hydroalcoholic extract of Moringa oleifera leaves for its Cardiotropic activity on albino Wistar rat. Research Journal of Pharmacy and Technology 2023; 16(7):3417-1. doi: 10.52711/0974-360X.2023.00565
Cite(Electronic):
Hira Das, Mrityunjoy Majumdar, Sourav Roy, Prodip Roy, Shristi Singh, Sohel Mondal, Asmita Das. Preclinical Evaluation of hydroalcoholic extract of Moringa oleifera leaves for its Cardiotropic activity on albino Wistar rat. Research Journal of Pharmacy and Technology 2023; 16(7):3417-1. doi: 10.52711/0974-360X.2023.00565 Available on: https://rjptonline.org/AbstractView.aspx?PID=2023-16-7-62
REFERENCES
1. Jain S, Buttar HS, et al. Prevention of CardiovascularDiseases with Anti-Inflammatory and Anti- Oxidant Nutraceuticals and HerbalProducts: An Overview of Pre-Clinical and ClinicalStudies. Recent Pat Inflamm Allergy DrugDiscov. 2018Oct 1;12(2):145-157.doi: 10.2174/1872213X12666180815144803
2. Cardiovascular diseases (CVDs), https://www.who.int/news-room/fact-sheets/detail/cardiovascular-diseases-(cvds): access on 30th June 2021.
3. Gupta N, et al. Review of Some Important Medicinal Plants Possesses Anti-Inflammatory Activity. Research Journal of Pharmacy and Technology. 2011;4(10):1506-1512
4. Prasad J, et al. An Ethnopharmacological Review: On Commonly used Anti-Oxidant Plants with Anti-Hypertensive. Research Journal of Pharmacology and Pharmacodynamics. 2018; 10(3):125-133. doi:10.5958/2321-5836.2018.00024.1
5. Majumdar M, et al. Study of wound healing activity of different formulations of Nigella sativa seed extract. Research Journal of Pharmacy and Technology. 2016 Dec 1; 9(12):2097.doi: 10.5958/0974-360X.2016.00427.3
6. Shekshavali T, Roshan S. Screening of Diuretic Activity of traditionally used Medicinal Plants. Research Journal of Pharmacology and Pharmacodynamics. 2017Dec 30; 9(4):178-80.doi: 10.5958/2321-5836.2017.00030.1
7. Saha S, et al. Preclinical Evaluation of Hydro Alcoholic Extract of fruits of Cascabela thevetia for its Cardio Toxic Activity. Research J. Pharm. and Tech 2019Aug 1; 12(8):3755-3758. doi: 10.5958/0974-360X.2019.00643.7
8. Introduction and importance of medicinal plants, https://www.nhp.gov.in/introduction-and-importance-of-medicinal-plants-and-herbs_mtl; access on 27th May 2021.
9. Meireles D, et al. A review of properties, nutritional and pharmaceutical applications of Moringa oleifera: integrative approach on conventional and traditional Asian medicine. Advances in Traditional Medicine. 2020 Dec;20(4):495-515. doi:10.1007/s13596-020-00468-0
10. Panya T, et al. Development and Evaluation of Lozenge from Moringa oleifera leafextract. Research J. Pharm. and Tech. 2016Jul 28; 9(7):805-809. doi: 10.5958/0974-360X.2016.00154.2
11. Moringa oleifera, https://en.wikipedia.org/wiki/Moringa_oleifera; access on 20th June, 2021.
12. Krishnaveni J,Ananthi T. Hepatoprotective Effect of Moringa oleifera in Isoniazid InducedRats. Research J. Pharm. and Tech.2011Dec 28; 4(12):1901-1903.
13. Estri M, et al. Red Moringa oleiferaleaffermentation extract protecting Hepatotoxicity in Balb/C mice injected with Salmonella typhi through Nrf-2, HO-1, and SOD-2 signalingpathways. Research J. Pharm. and Tech. 2020Dec 1; 13(12):5947-5952.doi: 10.5958/0974-360X.2020.01038.0
14. Charde RM, Charde MS, Fulzele SV, Satturwar PM, Kasture AV, Joshi SB. Evaluation of EthanolicExtract of Moringa Oleifera for WoundHealing, Anti-inflammatory and Antioxidant Activities on Rats. Research J. Pharm. and Tech.2011February; 4(2):254-258.
15. Mabrouki L,et al. Cardiac ameliorative effect of Moringa oleifera leafextract in high-fat diet-induced obesity in ratmodel. BioMed research international. 2020 Feb 28;2020:1-10.doi:10.1155/2020/6583603
16. Masum NHM, et al. In vitro AntioxidantActivities of Differentparts of the Plant Moringa oleifera Lam. Research J. Pharm. and Tech.2012 Dec 28; 5(12): 1532-1537.
17. Dhimmar N,et al.Pharmacological activities of Moringa oleifera: An Overview. Research J. Pharm. and Tech.2015Apr 1; 8(4):476-480. doi: 10.5958/0974-360X.2015.00079.7
18. Abd Rani NZ, et al. Moringa genus: a review of phytochemistry and pharmacology. Frontiers in Pharmacology. 2018 Feb 16;9:108. doi:10.3389/fphar.2018.00108
19. Karishma SK, et al. PharmacologicalEvaluation of LeafExtract of Terminalia bellerica with Moringa oleifera for its SynergisticAction on Anti-diabetic Activity and Anti-inflammatory Activity in Rats. Research J. Pharm. and Tech. 2019Mar 31; 12(3): 1181-1184.doi: 10.5958/0974-360X.2019.00195.1
20. Indurwade NH, et al. ComparativeStudy of Antimicrobial and AntioxidantActivity between Seeds and Leaves of CrudeDrug Moringa oleifera. Research J. Pharm. and Tech. 2019Apr 30; 12(4):1843-1848.doi: 10.5958/0974-360X.2019.00308.1
21. Okoye EI, et al. Formulation and Characterization of Moringa oleiferaLeaf Granules. I: MicromeriticProperties. Research J. Pharm. and Tech. 2013 Jan 28; 6(1): 66-74.
22. FatmawatiS, et al. Fermented Ethanolic Extract of Moringa oleifera leaves with Lactobacillus plantarum FNCC 0137 as Immunomodulators on Salmonella typhi-Infected Mice. Research J. Pharm. and Tech. 2020 Dec 1; 13(12): 5777-5782.doi: 10.5958/0974-360X.2020.01007.0
23. Kokate CK, Purohit AP, Gokhale SB. Text book of Pharmacognosy.Nirali Prakashan, Pune.2012.
24. Kulkarni SK. Hand book of experimental pharmacology. Vallabh Prakashan, New Delhi. 2012.
25. Tripathi KD. Essentials of medical pharmacology.Jaypee Brothers Medical Publishers (P) Ltd,New Delhi. 2015.
26. Rang HP, et al. Rang and Dale's pharmacology.Churchill Livingstone, Edinburgh. 2015.
REFERENCES
1. Jain S, Buttar HS, et al. Prevention of CardiovascularDiseases
with Anti-Inflammatory and Anti- Oxidant Nutraceuticals and HerbalProducts: An
Overview of Pre-Clinical and ClinicalStudies. Recent Pat Inflamm Allergy
DrugDiscov. 2018Oct 1;12(2):145-157.doi: 10.2174/1872213X12666180815144803
2. Cardiovascular diseases (CVDs),
https://www.who.int/news-room/fact-sheets/detail/cardiovascular-diseases-(cvds):
access on 30th June 2021.
3. Gupta N, et al. Review of Some Important
Medicinal Plants Possesses Anti-Inflammatory Activity. Research Journal of
Pharmacy and Technology. 2011;4(10):1506-1512
4. Prasad J, et al. An Ethnopharmacological
Review: On Commonly used Anti-Oxidant Plants with Anti-Hypertensive. Research
Journal of Pharmacology and Pharmacodynamics. 2018; 10(3):125-133.
doi:10.5958/2321-5836.2018.00024.1
5. Majumdar M, et al. Study of wound healing
activity of different formulations of Nigella sativa seed extract. Research
Journal of Pharmacy and Technology. 2016 Dec 1; 9(12):2097.doi:
10.5958/0974-360X.2016.00427.3
6. Shekshavali T, Roshan S. Screening of Diuretic
Activity of traditionally used Medicinal Plants. Research Journal of
Pharmacology and Pharmacodynamics. 2017Dec 30; 9(4):178-80.doi:
10.5958/2321-5836.2017.00030.1
7. Saha S, et al. Preclinical Evaluation of
Hydro Alcoholic Extract of fruits of Cascabela thevetia for its Cardio Toxic
Activity. Research J. Pharm. and Tech 2019Aug 1; 12(8):3755-3758. doi:
10.5958/0974-360X.2019.00643.7
8. Introduction and importance of medicinal
plants,
https://www.nhp.gov.in/introduction-and-importance-of-medicinal-plants-and-herbs_mtl;
access on 27th May 2021.
9. Meireles D, et al. A review of properties,
nutritional and pharmaceutical applications of Moringa oleifera: integrative
approach on conventional and traditional Asian medicine. Advances in
Traditional Medicine. 2020 Dec;20(4):495-515. doi:10.1007/s13596-020-00468-0
10. Panya T, et al. Development and Evaluation
of Lozenge from Moringa oleifera leafextract. Research J. Pharm. and Tech.
2016Jul 28; 9(7):805-809. doi: 10.5958/0974-360X.2016.00154.2
11. Moringa oleifera, https://en.wikipedia.org/wiki/Moringa_oleifera;
access on 20th June, 2021.
12. Krishnaveni J,Ananthi T. Hepatoprotective
Effect of Moringa oleifera in Isoniazid InducedRats. Research J. Pharm. and
Tech.2011Dec 28; 4(12):1901-1903.
13. Estri M, et al. Red Moringa oleiferaleaffermentation
extract protecting Hepatotoxicity in Balb/C mice injected with Salmonella typhi
through Nrf-2, HO-1, and SOD-2 signalingpathways. Research J. Pharm. and Tech.
2020Dec 1; 13(12):5947-5952.doi: 10.5958/0974-360X.2020.01038.0
14. Charde RM, Charde MS, Fulzele SV, Satturwar
PM, Kasture AV, Joshi SB. Evaluation of EthanolicExtract of Moringa Oleifera
for WoundHealing, Anti-inflammatory and Antioxidant Activities on Rats.
Research J. Pharm. and Tech.2011February; 4(2):254-258.
15. Mabrouki L,et al. Cardiac ameliorative
effect of Moringa oleifera leafextract in high-fat diet-induced obesity in
ratmodel. BioMed research international. 2020 Feb
28;2020:1-10.doi:10.1155/2020/6583603
16. Masum NHM, et al. In vitro
AntioxidantActivities of Differentparts of the Plant Moringa oleifera Lam.
Research J. Pharm. and Tech.2012 Dec 28; 5(12): 1532-1537.
17. Dhimmar N,et al.Pharmacological activities
of Moringa oleifera: An Overview. Research J. Pharm. and Tech.2015Apr 1;
8(4):476-480. doi: 10.5958/0974-360X.2015.00079.7
18. Abd Rani NZ, et al. Moringa genus: a review
of phytochemistry and pharmacology. Frontiers in Pharmacology. 2018 Feb
16;9:108. doi:10.3389/fphar.2018.00108
19. Karishma SK, et al.
PharmacologicalEvaluation of LeafExtract of Terminalia bellerica with Moringa
oleifera for its SynergisticAction on Anti-diabetic Activity and
Anti-inflammatory Activity in Rats. Research J. Pharm. and Tech. 2019Mar 31;
12(3): 1181-1184.doi: 10.5958/0974-360X.2019.00195.1
20. Indurwade NH, et al. ComparativeStudy of
Antimicrobial and AntioxidantActivity between Seeds and Leaves of CrudeDrug
Moringa oleifera. Research J. Pharm. and Tech. 2019Apr 30; 12(4):1843-1848.doi:
10.5958/0974-360X.2019.00308.1
21. Okoye EI, et al. Formulation and
Characterization of Moringa oleiferaLeaf Granules. I: MicromeriticProperties.
Research J. Pharm. and Tech. 2013 Jan 28; 6(1): 66-74.
22. FatmawatiS, et al. Fermented Ethanolic
Extract of Moringa oleifera leaves with Lactobacillus plantarum FNCC 0137 as
Immunomodulators on Salmonella typhi-Infected Mice. Research J. Pharm. and
Tech. 2020 Dec 1; 13(12): 5777-5782.doi: 10.5958/0974-360X.2020.01007.0
23. Kokate CK, Purohit AP, Gokhale SB. Text book
of Pharmacognosy.Nirali Prakashan, Pune.2012.
24. Kulkarni SK. Hand book of experimental
pharmacology. Vallabh Prakashan, New Delhi. 2012.
25. Tripathi KD. Essentials of medical
pharmacology.Jaypee Brothers Medical Publishers (P) Ltd,New Delhi. 2015.
26. Rang HP, et al. Rang and Dale's pharmacology.Churchill
Livingstone, Edinburgh. 2015.