Author(s): Bibhuti Bhusan Champati, Bhuban Mohan Padhiari, Asit Ray, Ambika Sahoo, Sudipta Jena, Tirthabrata Sahoo, Pratap Chandra Panda, Sanghamitra Nayak

Email(s): sanghamitran24@gmail.com

DOI: 10.52711/0974-360X.2023.00429   

Address: Bibhuti Bhusan Champati1, Bhuban Mohan Padhiari1, Asit Ray1, Ambika Sahoo1, Sudipta Jena1, Tirthabrata Sahoo2, Pratap Chandra Panda1, Sanghamitra Nayak1*
1Centre for Biotechnology, Siksha ‘O’ Anusandhan (Deemed to be University), Bhubaneswar - 751003, Odisha, India.
2Department of Botany, Science College, Konkarada, Ganjam - 761144, Odisha, India.
*Corresponding Author

Published In:   Volume - 16,      Issue - 6,     Year - 2023


ABSTRACT:
The steroidal saponin, Shatavarin IV is the major bioactive compound present in the root of Asparagus racemosus. Shatavarin IV contributes for a wide range of biological activities like antioxidant, antitussive, antidyspepsia, antiulcer and anticancer activity. The present investigation aimed to identify Shatavarin IV in five different species of the genus Asparagus. For the very first time, Shatavarin IV was identified in four species of Asparagus like Asparagus densiflorus, Asparagus setaceus, Asparagus plumosus and Asparagus sprengeri. The identification and quantification of Shatavarin IV was done by HPTLC analysis. The pre-coated silica gel 60 F254 plates were used as stationary phase for the development of chromatograms with ethyl acetate?methanol?water (7.5:1.5:1, v/v/v)) as mobile phase. The Shatavarin IV was detected at retardation factor (Rf) 0.55±0.05 and showed maximum absorption at 425nm. The method was validated for quantitative analysis and was found to be satisfactory. The six-point calibration curve of Shatavarin IV shows a standard deviation of 3.89 % with R2 value of 0.9968. The amount of Shatavarin IV were found to be 0.22%, 0.01%, 0.08%, 0.04% and 0.06% in A. racemosus, A. densiflorus, A. setaceus, A. plumosus and A. sprengeri respectively. The HPTLC Fingerprint developed in this research work could be useful for quality control and checking adulteration of all the five species of Asparagus. The presence of Shatavarin IV in other species of Asparagus would reduce the pressure on the Asparagus racemosus as theprimary source of drug thereby preserving its wild population.


Cite this article:
Bibhuti Bhusan Champati, Bhuban Mohan Padhiari, Asit Ray, Ambika Sahoo, Sudipta Jena, Tirthabrata Sahoo, Pratap Chandra Panda, Sanghamitra Nayak. Identification and quantification of Shatavarin IV in five different species of the genus Asparagus through a validated HPTLC method. Research Journal of Pharmacy and Technology 2023; 16(6): 2615-1. doi: 10.52711/0974-360X.2023.00429

Cite(Electronic):
Bibhuti Bhusan Champati, Bhuban Mohan Padhiari, Asit Ray, Ambika Sahoo, Sudipta Jena, Tirthabrata Sahoo, Pratap Chandra Panda, Sanghamitra Nayak. Identification and quantification of Shatavarin IV in five different species of the genus Asparagus through a validated HPTLC method. Research Journal of Pharmacy and Technology 2023; 16(6): 2615-1. doi: 10.52711/0974-360X.2023.00429   Available on: https://rjptonline.org/AbstractView.aspx?PID=2023-16-6-7


REFERENCES:
1.    Smita SS, Raj Sammi S, Laxman TS, Bhatta RS, Pandey R. Shatavarin IV elicits lifespan extension and alleviates Parkinsonism in Caenorhabditis elegans. Free Radical Research. 2017 Dec 2;51(11-12):954-69. https://doi.org/10.1080/10715762.2017.1395419
2.    Nagar BP, Dutt Garg V, Dhiman A. Ethnopharmacology, phytochemistry and bioactivity of Asparagus racemosus: an update. Pharmacologyonline. 2011;2:979-94.
3.    Alok S, Jain SK, Verma A, Kumar M, Mahor A, Sabharwal M. Plant profile, phytochemistry and pharmacology of Asparagus racemosus (Shatavari): A review. Asian Pacific Journal of Tropical Disease. 2013 Apr 1;3(3):242-51. https://doi.org/10.1016/S2222-1808(13)60049-3
4.    Gowthami R, Sharma N, Pandey R, Agrawal A. Status and consolidated list of threatened medicinal plants of India. Genetic Resources and Crop Evolution. 2021 Aug; 68(6):2235-63. https://doi.org/10.1007/s10722-021-01199-0
5.    Watharkar AD, Kadam SK, Khandare RV, Kolekar PD, Jeon BH, Jadhav JP, Govindwar SP. Asparagus densiflorus in a vertical subsurface flow phytoreactor for treatment of real textile effluent: a lab to land approach for in situ soil remediation. Ecotoxicology and Environmental Safety. 2018 Oct 15; 161:70-7. https://doi.org/10.1016/j.ecoenv.2018.05.078
6.    Mashele SS. Medicinal properties of selected asparagus species: a review. Phytochemicals in Human Health, 2019 Aug 9.
7.    Li SF, Wang J, Dong R, Zhu HW, Lan LN, Zhang YL, Li N, Deng CL, Gao WJ. Chromosome-level genome assembly, annotation and evolutionary analysis of the ornamental plant Asparagus setaceus. Horticulture Research. 2020 Dec 1; 7:1-7.https://doi.org/10.1038/s41438-020-0271-y
8.    Fonnesbech M, Fonnesbech A, Bredmose N.Development of Asparagus plumosus shoot tips grown in vitro. Physiologia Plantarum. 1977 Jun; 40(2):73-6.https://doi.org/10.1111/j.1399-3054.1977.tb01497.x
9.    Sharma SC, Sharma R, Kumar R. Spirostanosides of Asparagus sprengeri. Phytochemistry. 1983 Jan 1;22(10):2259-62. https://doi.org/10.1016/S0031-9422(00)80157-0
10.    Mohamed MA. Spirostanol saponins from Asparagus sprengeri and their molluscicidal activity. Natural Product Communications. 2007 Jul;2(7):731-736 https://doi.org/10.1177/1934578X0700200705
11.    Kadhim EJ, Salah Z (2014) Phytochemical investigation & antibacterial activity of the essential oils from two species of Asparagus (Asparagus officinalis & Asparagus sprengeri) family Liliaceae cultivated in Iraq. Pharmacie Globale. 2014 Jul 1;5(3):1-4
12.    Goyal RK, Singh J, Lal H. Asparagus racemosus-an update. Indian Journal of Medical Sciences. 2003 Sep 1;57(9):408-14.
13.    International Conference on Harmonization (2005) ICH Q2 (R1): Validation of Analytical Procedures: Text and Methodology, ICH Secretariat, Geneva.
14.    Mosic M, Dramicanin A, Ristivojevic P, Milojkovic-OpsenicaD. Extraction as a Critical Step in Phytochemical Analysis. Journal of AOAC International. 2020 Mar;103(2):365-72.
15.    Le VA, Parks ES, Nguyen HM, Roach DP. Effect of solvents and extraction methods on recovery of bioactive compounds from defatted Gac (Momordica cochinchinensis Spreng.) seeds. Separations. 2018 Sep;5(3):39. https://doi.org/10.3390/separations5030039
16.    Barve KH, Laddha KS, Jayakumar B. Extraction of saponins from Safed Musli. Pharmacognosy Journal. 2010 Sep 1;2(13):561-4.https://doi.org/10.1016/S0975-3575(10)80060-4
17.    Zhao Y, Wang X, Wang H, Liu T, Xin Z. Two new noroleanane-type triterpene saponins from the methanol extract of Salicornia herbacea. Food Chemistry. 2014 May 15;151:101-9. https://doi.org/10.1016/j.foodchem.2013.11.030
18.    Dawid C, Hofmann T. Structural and sensory characterization of bitter tasting steroidal saponins from asparagus spears (Asparagus officinalis L.). Journal of Agricultural and Food Chemistry. 2012 Dec 5;60(48):11889-900.https://doi.org/10.1021/jf304085j
19.    Haghi G, Hatami A, Mehran M. Determination of Shatavarin IV in Root Extracts of Asparagus racemosus by HPLC-UV. Analytical Chemistry Letters. 2012 Jan 1;2(1):1-6. https://doi.org/10.1080/22297928.2012.10648246
20.    Ahmad MP, Hussain A, Wahab S, Ansari AA, Singh S, Mishra C, Ahmad S. Pharmacognostical and Phytochemical evaluation of root of Asparagus racemosus Willd. Journal of Drug Delivery and Therapeutics. 2017 Nov 12;7(6):76-80. https://doi.org/10.22270/jddt.v7i6.1524
21.    Hazra K, Mandal AK, Mondal DN, Ravte RK, Hazra J, Rao MM. Seasonal dynamics of Shatavarin-IV, a potential biomarker of Asparagus racemosus by HPTLC: Possible validation of the ancient Ayurvedic text. Indian Journal of Traditional Knowledge (IJTK). 2019 Dec 27;19(1):174-81.
22.    Haldar S, Mohapatra S, Singh R, Katiyar CK. Quantitative evaluation of shatavarin IV by high-performance thin-layer chromatography and its isolation from Asparagus racemosus Willd. JPC-Journal of Planar Chromatography-Modern TLC. 2018 Jun;31(3):197-201. https://doi.org/10.1556/1006.2018.31.3.3
23.    Mohamad M, Ali MW, Ahmad A. Modelling for extraction of major phytochemical components from Eurycoma longifolia. Journal of Applied Sciences. 2010 Dec;10(21):2572-7.
24.    Karlund A, Moor U, Sandell M, Karjalainen RO. The impact of harvesting, storage and processing factors on health-promoting phytochemicals in berries and fruits. Processes. 2014 Sep;2(3):596-624. https://doi.org/10.3390/pr2030596
25.    Gautam M, Saha S, Bani S, Kaul A, Mishra S, Patil D, Satti NK, Suri KA, Gairola S, Suresh K, Jadhav S. Immunomodulatory activity of Asparagus racemosus on systemic Th1/Th2 immunity: implications for immunoadjuvant potential. Journal of Ethnopharmacology. 2009 Jan 21;121(2):241-7. https://doi.org/10.1016/j.jep.2008.10.028
26.    Thomas MT, Kurup R, Johnson AJ, Chandrika SP, Mathew PJ, Dan M, Baby S. Elite genotypes/chemotypes, with high contents of madecassoside and asiaticoside, from sixty accessions of Centella asiatica of south India and the Andaman Islands: for cultivation and utility in cosmetic and herbal drug applications. Industrial Crops and Products. 2010 Nov 1;32(3):545-50.https://doi.org/10.1016/j.indcrop.2010.07.003
27.    Saran PL, Singh S, SolankiV, Choudhary R,Manivel P. Evaluation of Asparagus adscendens accessions for root yield and shatavarin IV content in India. Turkish Journal of Agriculture and Forestry. 2021 Aug 18;45(4):475-83.
28.    Madhavan V, Tijare RD, Mythreyi R, Gurudeva MR, Yoganarasimhan SN. Pharmacognostical studies on the root tubers of Asparagus gonoclados Baker–Alternate source for the Ayurvedic drug Shatavari. Indian Journal of Natural Products and Resources. 2010 Mar;1(1):57-62.
29.    Tijare RD, Beknal AB, Mahurkar N, Chandy V. Pharmacognostical and phytochemical studies of root tubers of Asparagus gonoclados Baker. International Journal of Pharmacognosy and Phytochemical Research 4 (3). 2012:142-5.
30.    Ram M, Abdin MZ, Khan MA, Jha P. HPTLC fingerprint analysis: a quality control for authentication of herbal phytochemicals. Inhigh-performance thin-layer chromatography (HPTLC) 2011 (pp. 105-116). Springer, Berlin, Heidelberg.https://doi.org/10.1007/978-3-642-14025-9_7
31.    Nicoletti M. HPTLC fingerprint: a modern approach for the analytical determination of botanicals. Revista Brasileira de Farmacognosia. 2011 Oct;21(5):818-23. https://doi.org/10.1590/S0102-695X2011005000131
32.    Meena AK, Narasimhaji CV, Velvizhi D, Singh A, Rekha P, Kumar V, Ilavarasan R, Srikanth N, Dhiman KS. Determination of gallic acid in Ayurvedic Polyherbal formulation Triphala churna and its ingredients by HPLC and HPTLC. Research Journal of Pharmacy and Technology. 2018;11(8):3243-9.https://doi.org/10.5958/0974-360X.2018.00596.6
33.    Kharat SN, Ansari N, Mendhulkar VD. HPTLC screening for Flavonoids content in leaf extracts of Syzygium cumini (Linn.) and its Antimicrobial activity. Research Journal of Pharmacy and Technology. 2020;13(6):2720-6.https://doi.org/10.5958/0974-360X.2020.00484.9
34.    Daharwal SJ, Shrivastava S. Preliminary Phytochemical Screening and HPTLC Fingerprinting of Extracts of Thuja occidentalis. Research Journal of Pharmacy and Technology. 2019;12(10):4782-4.https://doi.org/10.5958/0974-360X.2019.00825.4
35.    Deep P, Mishra A, Verma N. Pharmacognostical Evaluation and HPTLC Analysis of Two Medicinally important Secondary Metabolites in Bauhinia variegata Leaves from Gorakhpur District in Summer Season. Research Journal of Pharmacy and Technology. 2020;13(6):2667-71.https://doi.org/10.5958/0974-360X.2020.00474.6
36.    Rajasekaran A, Arivukkarasu R, Bonagiri R, Saradhi RP. Antimicrobial evaluation and quantification of apigenin content by HPTLC in methanol stem extract of Cardiospermum halicacabum L. Research Journal of Pharmacy and Technology. 2014 May 28;7(5):537-43.
37.    Sampathkumar Y, Mahadevan SG, Jayaraman R. Physicochemical, Phytochemical screening and HPTLC Fingerprinting Analysis of Ethanolic extract of Mimusops elengi Linn. leaves. Research Journal of Pharmacy and Technology. 2020;13(5):2091-5.https://doi.org/10.5958/0974-360X.2020.00376.5
38.    Raj A, Vinnarasi J, Venkataraman R, Augustin M. HPTLC Fingerprinting Analysis of Tannin Profile on Canthium coromandelicum and Flueggea leucopyrus willd. Research Journal of Pharmacy and Technology. 2018;11(12):5355-8.https://doi.org/10.5958/0974-360X.2018.00975.7
39.    Goswami S, Singh RP, Gilhotra RM. HPTLC Fingerprinting of Quercetin and comparative assessment of Anthelmintic potency against Eudrilus eugeniae of Schleichera oleosa (Lour) Oken and Tagetes eretca Linn. Research Journal of Pharmacy and Technology. 2020 Dec 1;13(12):5699-704.https://doi.org/10.5958/0974-360X.2020.00992.0
40.    Shelar KU, Rao JR, Dhale C. Stability indicating HPTLC method development and validation for the estimation of celecoxib in bulk drug and its pharmaceutical formulation. Research Journal of Pharmacy and Technology. 2020;13(8):3661-5.https://doi.org/10.5958/0974-360X.2020.00647.2
41.    Tamilselvi N, Arivukkarasu R, Suresh P, Suriyan N, Thiramilan A, Valarmathi C. Method Development and Validation of Ezogabine by using HPTLC Method. Research Journal of Pharmacy and Technology. 2019 Dec 30;3(5.5):1-5.https://doi.org/10.5958/0974-360X.2019.00985.5

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