Author(s):
Suhartono Suhartono, Zinatul Hayati, Rika Hayatunnida
Email(s):
suhartono@unsyiah.ac.id
DOI:
10.52711/0974-360X.2023.00470
Address:
Suhartono Suhartono1*, Zinatul Hayati2, Rika Hayatunnida1
1Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Jl. Tgk. Syech Abdurrauf No. 3, Darussalam, Banda Aceh, 23111, Indonesia.
2Microbiology Department, Faculty of Medicine, Universitas Syiah Kuala, Jl. T. Tanoeh Abe, Darussalam, Banda Aceh, 23111, Indonesia.
*Corresponding Author
Published In:
Volume - 16,
Issue - 6,
Year - 2023
ABSTRACT:
Inappropriate antibiotic use and diagnosis might contribute to the increasing occurrences of multidrug resistant (MDR) Escherichia coli and Klebsiella pneumoniae infections in both the community and hospitals settings. This study aimed to determine the distribution and antibiotic susceptibility of MDR Escherichia coli and Klebsiella pneumoniae based on clinical specimens, time interval, and patients’ gender at RSUDZA during a study period of February 2019-January 2021. Methods Isolation of Escherichia coli and Klebsiella pneumoniae was carried out using culture technique on blood agar and MacConkey agar. Identification of Escherichia coli and Klebsiella pneumoniae and antibiotic sensitivity tests were performed using VITEK® 2 Compact. The results showed that during the study period, there were a total of 669 isolates of Escherichia coli obtained with a percentage of MDR E. coli of 30.90% (208 isolates); and 525 isolates of Klebsiella pneumoniae with a percentage of MDR K. pneumoniae of 25.15% (132 isolates). Both MDR E. coli and MDR K. pneumoniae isolates showed their resistance to more than three classes of antibiotics. Based on clinical specimens, both MDR E. coli and K. pneumoniae were predominantly found in urine specimens, namely 122 isolates (58.65%) and 49 isolates (37.12%), respectively. Based on gender, MDR E. coli infection was commonly found in women, namely 114 isolates (54.80%), whereas MDR K. pneumoniae infection was commonly found in men, namely 72 isolates (54.13%). Overall, both MDR E. coli and K. pneumoniae were predominantly found as pathogens especially causing urinary tract infections in female patients during the time period of study. This study may suggest to effectively implement the surveillance programs and infections control in order to manage these MDR bacterial infections particularly within healthcare settings.
Cite this article:
Suhartono Suhartono, Zinatul Hayati, Rika Hayatunnida. Distribution of Multidrug Resistant Escherichia coli and Klebsiella pneumoniae Isolates among clinical specimens in the Zainoel Abidin General Hospital, Banda Aceh, Indonesia. Research Journal of Pharmacy and Technology 2023; 16(6):2854-8. doi: 10.52711/0974-360X.2023.00470
Cite(Electronic):
Suhartono Suhartono, Zinatul Hayati, Rika Hayatunnida. Distribution of Multidrug Resistant Escherichia coli and Klebsiella pneumoniae Isolates among clinical specimens in the Zainoel Abidin General Hospital, Banda Aceh, Indonesia. Research Journal of Pharmacy and Technology 2023; 16(6):2854-8. doi: 10.52711/0974-360X.2023.00470 Available on: https://rjptonline.org/AbstractView.aspx?PID=2023-16-6-48
REFERENCES:
1. Serra-Burriel M. Keys M. Campillo-Artero C et al. Impact of Multi-drug Resistant Bacteria on Economic and Clinical Outcomes of Healthcare-associated Infections in Adults: Systematic Review and Meta-Analysis. PLOS ONE. 2020;15(1):e0227139. DOI: https://doi.org/10.1371/journal.pone.0227139
2. Virawan H. Nuryastuti T. Nirwati H. Multidrug-resistant Klebsiella pneumoniae from Clinical Isolates at dr. Soeradji Tirtonegoro Central Hospital Klaten. Jurnal Kedokteran dan Kesehatan Indonesia. 2020;11(2):109-20. DOI: https://doi.org/10.20885/jkki.vol11.iss2.art3
3. Sidjabat HE. Paterson DL. Multidrug-resistant Escherichia coli in Asia: Epidemiology and Management. Expert Review of Anti-infective Therapy. 2015;13(5):575-91. DOI: https://doi.org/10.1586/14787210.2015.1028365
4. Moini AS. Soltani B. Taghavi Ardakani A et al. Multidrug-resistant Escherichia coli and Klebsiella pneumoniae Isolated from Patients in Kashan, Iran. Jundishapur Journal of Microbiology. 2015;8(10):e27517-e17. DOI: https://doi.org/10.5812/jjm.27517
5. Rajan AK, Jeyamani SVP. Assessment of antibiotic sensitivity patterns in a primary care hospital. Research Journal of Pharmacy and Technology. 2018;11(8):3411-14. DOI: https://doi.org/10.5958/0974-360X.2018.00628.5
6. Omran ST, Mohammed-Jawad NK, Fareed NY. Population knowledge about rational use of antibiotics and antibiotics resistance in Basrah, Iraq. Research Journal of Pharmacy and Technology. 2021;14(12):6659-64. DOI: https://doi.org/10.52711/0974-360X.2021.01150
7. Niveditha S, Umamageswari S, Aruna D, Kalyani M. Study of hand carriage of multi drug resistant bacteria using glove juice technique in health care workers. Research Journal of Pharmacy and Technology. 2021;14(2):650-56. DOI: https://doi.org/10.5958/0974-360X.2021.00116.5
8. Sreeja MK, Gowrishankar NL, Adhisa S, Divya KC. Antibiotic resistance-reasons and the most common resistant pathogens–a review. Research Journal of Pharmacy and Technology. 2017;10(6):1886-90. DOI: https://doi.org/10.5958/0974-360X.2017.00331.6
9. Al-Hilali SAM, Hadi ZJ, Aljayashi KG. Prevalence of plasmid-mediated quinolone resistance genes among ciprofloxacin-nonsusceptible Escherichia coli and Klebsiella pneumoniae isolated from clinical isolates in Najaf, Iraq. Research Journal of Pharmacy and Technology. 2021;14(4):1966-72. DOI: https://doi.org/10.52711/0974-360X.2021.00348
10. Sjahjadi NR. Rasyid R. Rustam E. Restusari L. Prevalensi Kuman Multi Drug Resistance (MDR) di Laboratorium Mikrobiologi RSUP dr. M. Djamil Padang periode Januari 2010-Desember 2012. Jurnal Kesehatan Andalas. 2014;3(3):440-4 DOI: https://doi.org/10.25077/jka.v3i3.169
11. Abbas HA, Kadry AA, Shaker GH, Goda RM. Resistance of Escherichia coli and Klebsiella pneumoniae isolated from different sources to β-lactam antibiotics. Research Journal of Pharmacy and Technology. 2017;10(2):589-91 DOI: https://doi.org/10.5958/0974-360X.2017.00116.0
12. Stanley IJ. Kajumbula H. Bazira J. Kansiime C. Rwego IB. Asiimwe BB. Multidrug Resistance among Escherichia coli and Klebsiella pneumoniae Carried in the Gut of Out-patients from Pastoralist Communities of Kasese District, Uganda. PLOS ONE. 2018;13(7):e0200093. DOI: https://doi.org/10.1371/journal.pone.0200093
13. Baaity Z, Almahmoud I, Khamis A. Prevalence of extended spectrum β lactamases (ESBL) in E. coli at Al-Assad teaching hospital. Research Journal of Pharmacy and Technology. 2017;10(7):2433-36. https://doi.org/10.5958/0974-360X.2017.00430.9
14. Hariharan P. Bharani T. Franklyne JS. Biswas P. Solanki SS. Paul-Satyaseela M. Antibiotic Susceptibility Pattern of Enterobacteriaceae and Non-fermenter Gram-negative Clinical Isolates of Microbial Resource Orchid. Journal of Natural Science, Biology and Medicine 2015;6(1):198-201. DOI: https://doi.org/10.4103/0976-9668.149121
15. Bien TLT. Sato-Takabe Y. Ogo M. Usui M. Suzuki S. Persistence of Multi-drug Resistance Plasmids in Sterile Water under very low Concentrations of Tetracycline. Microbes and Environments. 2015;30(4):339-43. DOI: https://doi.org/10.1264/jsme2.me15122
16. Venkateswarlu D, Pandian A, Ramesh S. Detection of β-lactamase in clinical samples of Prakasam district,Andhra Pradesh. Research Journal of Pharmacy and Technology. 2021;14(6):2995-98. DOI: https://doi.org/10.52711/0974-360X.2021.00524
17. Mancy AS, Shaheen S, Albaghdady A, Sabri NA. Efficacy and safety of colistin-imipenem/cilastatin combination therapy for multidrug-resistant gram-negative bacteria infections in critically ill pediatric patients. Research Journal of Pharmacy and Technology. 2022;15(3):1059-63. DOI: https://doi.org/10.52711/0974-360X.2022.00177
18. Aminul P. Anwar S. Molla MMA. Miah MRA. Evaluation of Antibiotic Resistance Patterns in Clinical Isolates of Klebsiella pneumoniae in Bangladesh. Biosafety and Health. 2021;3(6):301-6. DOI: https://doi.org/10.1016/j.bsheal.2021.11.001
19. Al-Faqeeh LAS, Naser R, Kagne S. Determination of antibiotic resistant profiles for bacteria isolated from clinical samples in Aurangabad, India. Research Journal of Pharmacy and Technology. 2020;13(8):3813-16. DOI: https://doi.org/10.5958/0974-360X.2020.00675.7
20. Derakhshan S. Najar Peerayeh S. Bakhshi B. Association between Presence of Virulence Genes and Antibiotic Resistance in Clinical Klebsiella pneumoniae Isolates. Laboratory medicine. 2016;47(4):306-11. DOI: https://doi.org/10.1093/labmed/lmw030
21. Firoozeh F. Mahluji Z. Khorshidi A. Zibaei M. Molecular Characterization of Class 1, 2 and 3 Integrons in Clinical Multi-drug Resistant Klebsiella pneumoniae isolates. Antimicrobial Resistance & Infection Control. 2019;8(1):1-7. DOI: https://doi.org/10.1186/s13756-019-0509-3
22. Jiang Y. Wang Y. Hua X. Qu Y. Peleg AY. Yu Y. Pooled Plasmid Sequencing Reveals the Relationship between Mobile Genetic Elements and Antimicrobial Resistance Genes in Clinically Isolated Klebsiella pneumoniae. Genomics, proteomics & bioinformatics. 2020;18(5):539-48. DOI: https://doi.org/10.1016/j.gpb.2020.12.002
23. Ahmed SS. Shariq A. Alsalloom AA. Babikir IH. Alhomoud BN. Uropathogens and their Antimicrobial Resistance Patterns: Relationship with Urinary Tract Infections. International Journal of Health Sciences (Qassim). 2019;13(2):48-55. https://pubmed.ncbi.nlm.nih.gov/30983946/
24. Shaifali I. Gupta U. Mahmood SE. Ahmed J. Antibiotic Susceptibility Patterns of Urinary Pathogens in Female Outpatients. North American Journal of Medical Sciences. 2012;4(4):163-9. DOI: https://doi.org/10.4103/1947-2714.94940
25. Suhartono S. Mahdani W. Hayati . Nurhalimah N. Species Distribution of Enterobacteriaceae and Non-enterobacteriaceae Responsible for Urinary Tract Infections at the Zainoel Abidin Hospital In Banda Aceh, Indonesia. Biodiversitas Journal of Biological Diversity. 2021;22(8):3311-8. DOI: https://doi.org/10.13057/biodiv/d220826
26. López‐de‐Andrés A. Albaladejo‐Vicente R. de Miguel‐Diez J. et al. Gender Differences in Incidence and In‐hospital Outcomes of Community‐Acquired, Ventilator‐Associated and Nonventilator Hospital‐acquired Pneumonia in Spain. International Journal of Clinical Practice. 2021;75(3):e13762. DOI: https://doi.org/10.1111/ijcp.13762