Author(s):
Vishal Gupta, Pallavi L C, Serenn Rose Thomson, Balaji O, Shivaprakash Gangachannaiah
Email(s):
shiva.g@manipal.edu
DOI:
10.52711/0974-360X.2023.00459 
Address:
Vishal Gupta1, Pallavi L C2, Serenn Rose Thomson1, Balaji O1, Shivaprakash Gangachannaiah1*
1Department of Pharmacology, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, India.
2Department of Physiology, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, India.
*Corresponding Author
Published In:
Volume - 16,
Issue - 6,
Year - 2023
ABSTRACT:
Betel nut is one of the most common addictive substance. Not many studies have explored the potential of varenicline as cessation therapy. We studied the benefits of varenicline in reducing the reward effects in rat model. Forty male albino rats of wistar strain weighing 150-250grams were used in the present study. Rats were randomized to 5 groups of 8 rats each to: Control; Betel nut group; Varenicline at doses of 0.5, 1 and 2mg/kg along with betel nut. Conditioned Place Preference (CPP) model was used for studying the drug seeking behavior. The CPP score was compared between the groups. Results showed a significant increase in body weight of rats at the end of the study compared to baseline in betel nut and varenicline groups. Compared to betel nut group (656.25±55) there was significant decrease in CPP score in Varenicline group at 0.5mg/kg (97.5±48); 1mg/kg (18.75±20) and at dose of 2mg/kg (-60±6). The study demonstrates the benefits of varenicline in betelnut dependence. There was dose dependent decrease in betel nut seeking behavior with varenicline in rat model of drug dependence.
Cite this article:
Vishal Gupta, Pallavi L C, Serenn Rose Thomson, Balaji O, Shivaprakash Gangachannaiah. The effect of Varenicline on Betel nut dependence in Albino wistar rats. Research Journal of Pharmacy and Technology 2023; 16(6):2791-4. doi: 10.52711/0974-360X.2023.00459
Cite(Electronic):
Vishal Gupta, Pallavi L C, Serenn Rose Thomson, Balaji O, Shivaprakash Gangachannaiah. The effect of Varenicline on Betel nut dependence in Albino wistar rats. Research Journal of Pharmacy and Technology 2023; 16(6):2791-4. doi: 10.52711/0974-360X.2023.00459 Available on: https://rjptonline.org/AbstractView.aspx?PID=2023-16-6-37
REFERENCES:
1. Baviskar HP, Dhake GT, Kasai MA, Chaudhari NB, Deshmukh TA. Review of Piper Betle. Res. J. Pharmacognosy and Phytochem. 2017; 9(2): 128-134. doi: 10.5958/0975-4385.2017.00024.3
2. Nishmitha Gretta Dsouza, Jennifer Fernandes, Sonal D’Souza, Ronald Fernandes. In Vitro Antioxidant Activity of Leaves Extracts of Areca catechu. Research J. Pharm. and Tech. 2019; 12(4): 1536-1540. doi: 10.5958/0974-360X.2019.00254.3
3. Neeraj S Vyawahare, Virendra G Kagathara, RG Katedeshmukh, Pushpendra K Sharma, Smeeta M Mohod. Evaluation of Antiulcer Activity of Piper betel Leaves Extract in Rats. Research J. Pharmacology and Pharmacodynamics. 2010; 2(4): 278-282. Available on: https://rjppd.org/AbstractView.aspx?PID=2010-2-4-16
4. Nem Kumar Jain, Rituparna Roy, Hero Khan Pathan, Aditi Sharma, Shakhi Ghosh, Santosh Kumar. Formulation and Evaluation of Polyherbal Aqueous Gel from Psidium guajava, Piper betel and Glycerrhiza glabra Extract for Mouth Ulcer Treatment. Res. J. Pharmacognosy and Phytochem. 2020; 12(3):145-148. doi: 10.5958/0975-4385.2020.00024.2
5. K. Radhika, B. Kumaravel, V. Thamizhiniyan, S. Subramanian. Biochemical evaluation of antidiabetic activity of Piper betel leaves extract in alloxan-induced diabetic rats. Asian J. Research Chem. 6(1): January 2013; Page 76-82. Available on: https://ajrconline.org/AbstractView.aspx?PID=2012-5-1-32
6. Abdullah Ansari, Tarique Mahmood, Paramdeep Bagga, Farogh Ahsan, Arshiya Shamim, Shoaib Ahmad, Mohammad Shariq, Saba Parveen. Catechism of protective potential of Areca catechu in Isoprenaline challenged Wistar rats. Research Journal of Pharmacy and Technology. 2021; 14(6):3067-3. doi: 10.52711/0974-360X.2021.00536
7. Nishant. M. Patel, H. P. Suryawanshi, S. P. Pawar. Phytopharmacological Study of Areca catechu leaf. Res. J. Pharmacology and Pharmacodynamics. 2019; 11(3):95-100. doi: 10.5958/2321-5836.2019.00017.X
8. V.A. Kangralkar, A.R. Kulkarni. Evaluation of effect of Piper betel, Centella asiatica and Aristolochia indica extracts on bacterial enzymes in 1, 2-dimethyl hydrazine induced colon cancer in wistar rats. Research J. Pharm. and Tech. 7(2): Feb. 2014; Page 151-154. Available on: https://rjptonline.org/AbstractView.aspx?PID=2014-7-2-23
9. B. Usharani, R. Monisha. In-vitro Cytotoxicity assay of Betel quid extract against HeLa cells. Research Journal of Pharmacy and Technology. 2022; 15(1):47-0. doi: 10.52711/0974-360X.2022.00009
10. Rutuja R Shah, Poonam J Patil, Pratibha R Adnaik, Rahul S Adnaik. Biochemical profiling of Antifungal soap activity of betel leaf (Piper betle L.) extract and Garlic oil by In vitro method. Asian J. Pharm. Res. 2020; 10(4):260-262. doi:10.5958/2231-5691.2020.00045.3
11. Anju Mary Daniel, Eby Aluckal, Giju Baby George, Mathews Baby. Etiopathogenisis of Oral Sub Mucous Fibrosis. Res. J. Pharmacology and Pharmacodynamics.2017; 9(3): 167-170. doi: 10.5958/2321-5836.2017.00028.3
12. Papke RL, Horenstein NA, Stokes C. Nicotinic Activity of Arecoline, the Psychoactive Element of" Betel Nuts", Suggests a Basis for Habitual Use and Anti-Inflammatory Activity. PloS one. 2015 Oct 21;10(10):e0140907. doi: 10.1371/journal.pone.0140907
13. O’Connor EC, Parker D, Rollema H, Mead AN. The α4β2 nicotinic acetylcholine-receptor partial agonist varenicline inhibits both nicotine self-administration following repeated dosing and reinstatement of nicotine seeking in rats. Psychopharmacology. 2010 Feb 1; 208(3):365-76. doi: 10.1007/s00213-009-1739-5
14. Cunnigham CL, Gremel CM, Groblewski PA. Drug-induced conditioned place preference and aversion in mice. Nature protocols 2006;1(4):1662-1670. doi:10.1038/nprot.2006.279
15. Burton-Bradley, Talonu. Betel quid and betel nut chewing and some betel nut derived nitrosamines.IARC Working Group on the Evaluation of Carcinogenic Risks to Humans, International Agency for Research on Cancer Vol 85. France; 2004. p 173. http://monographs.iarc.fr/ENG/Publications/corrigenda.php
16. Champix: EPAR - Scientific Discussion - European Medicines Agency. (1998, September 4). Retrieved January 17, 2017, from http://www.ema.europa.eu/docs/en_GB/document_library/EPAR_-_Scientific_Discussion/human/000699/WC500025254.pdf
17. Biala G, Staniak N, Budzynska B. Effects of varenicline and mecamylamine on the acquisition, expression, and reinstatement of nicotine- conditioned place preference by drug priming in rats. Naunyn-Schmiedeberg’s archives of pharmacology. 2010; 381(4);361-370. doi: 10.1007/s00210-010-0498-5
18. Mohammad Perwaiz Iqbal, Naseema Mehboobali, Ghulam Haider, and et al. Effects of betel nut on cardiovascular risk factors in a rat model. BMC Cardiovascular Disorders 2012, 12:94. doi: 10.1186/1471-2261-12-94
19. Lohith T.S, Shridhar N.B, Dilip S.M and et al. Repeated dose 28-day oral toxicity study of raw areca nut extract in rats. International Research Journal of Pharmacy 2013; 4(5):238-240. doi: 10.7897/2230-8407.04550
20. Wen-Yuan Lin, F. Xavier Pi-Sunyer, Chiu-Shong Liu, and et al. Betel Nut Chewing Is Strongly Associated With General and Central Obesity in Chinese Male Middle-aged Adults. Obesity (Silver Spring). 2009 June; 17(6): 1247–1254. doi:10.1038/oby.2009.38
21. Boucher BJ, Mannan N. Metabolic effects of the consumption of Areca catechu. Addict Biol. 2002 Jan;7(1):103-10. doi: 10.1080/13556210120091464. PMID: 11900629.
22. Selçuk EB, Sungu M, Parlakpinar H, and et al. Evaluation of the cardiovascular effects of varenicline in rats. Drug Design, Development and Therapy 2015:9; 5705–5717. doi https://doi.org/10.2147/DDDT.S92268