Author(s):
Anindita Sarma, Sanjib Kalita, Sudem Wary, Satya Nath Doley, Kumanand Tayung
Email(s):
kumanand@gauhati.ac.in
DOI:
10.52711/0974-360X.2023.00424 
Address:
Anindita Sarma1, Sanjib Kalita1, Sudem Wary1, Satya Nath Doley2, Kumanand Tayung1*
1Mycology and Plant Pathology Laboratory, Department of Botany, Gauhati University, Guwahati, Assam – 781014.
2Department of Botany, Arya Vidyapeeth College, Guwahati, Assam – 781016.
*Corresponding Author
Published In:
Volume - 16,
Issue - 6,
Year - 2023
ABSTRACT:
Even though modern medicinal system is well developed and easily accessible, the rural people still continues to depend upon traditional medicinal system for curing various ailments. Several cases of fast recovery and many cases of death due to wrong treatment was observed during the year 2018-19 in rural villages of Nagaon district of Assam, India. The objective of this study was to evaluate the efficacy of some medicinal plants and their formulations used by traditional healer for treatment of gastrointestinal disorders. The information was collected by interaction with the traditional healers and altogether 14 plant species were documented for preparation of six different formulations. Antibacterial evaluation against E. coli, P. aeruginosa and S. aureus revealed that formulation prepared from freshly prepared plant materials showed better efficacy than formulations obtained from traditional healers. Again, organic solvents extracts showed greater antibacterial activity than the aqueous extracts. Amongst the solvents, the polar organic solvent methanol showed greater inhibitory effect as compared to other non polar organic solvents, ethanol and hexane. GC-MS analysis of the potent plant extracts showed presence of several bioactive compounds like tumerone, curlone, hexadecanoic acid, oleic acid, eicosanoic acid, erucic acid and isopimaric acid which have earlier reports of having significant antibacterial activities.
Cite this article:
Anindita Sarma, Sanjib Kalita, Sudem Wary, Satya Nath Doley, Kumanand Tayung. Antibacterial Evaluation of some traditionally used medicinal plants and their Formulations for treatment of Gastro Intestinal Disorders. Research Journal of Pharmacy and Technology 2023; 16(6):284-0. doi: 10.52711/0974-360X.2023.00424
Cite(Electronic):
Anindita Sarma, Sanjib Kalita, Sudem Wary, Satya Nath Doley, Kumanand Tayung. Antibacterial Evaluation of some traditionally used medicinal plants and their Formulations for treatment of Gastro Intestinal Disorders. Research Journal of Pharmacy and Technology 2023; 16(6):284-0. doi: 10.52711/0974-360X.2023.00424 Available on: https://rjptonline.org/AbstractView.aspx?PID=2023-16-6-2
REFERENCES:
1. Acharya KP. Rokaya MB. Ethnobotanical survey of medicinal plants traded in the streets of Kathmandu valley. Scientific World. 2005; 3(3):44-48.
2. Perampaladas K. Masum H. Kapoor A. Shah R. Daar AS. Singer PA. The road to commercialization in Africa: lessons from developing the sickle-cell drug Niprisan. BMC International Health and Human Right. 2010; 10(1):1-7. doi: 10.1186/1472-698X-10-S1-S11.
3. Soni S. Kumar P. Verma VS. Sharma M. Significance of Indian medicinal plants used for treatment of dementia. Asian Journal of Research in Pharmaceutical Science. 2014; 4(4): 202-205.
4. Mandal SC. Chakraborty R. Sen S. Evidence Based Validation of Traditional Medicines: a comprehensive approach. Springer Singapore. 2021.
5. Kumudhaveni B. Radha R. Anti-diabetic potential of a traditional Polyherbal Formulation – A Review. Research Journal of Pharmacy and Technology. 2017; 10(6):1865-1869. doi: 10.5958/0974-360X.2017.00327.4.
6. Coburn B. Some native medicinal plants of the western Gurung. Kailash. 1984; 11(1-2): 55-88.
7. Rokaya MB. Uprety Y. Poudel RC. Timsina B. Münzbergová Z. Asselin H. Tiwari A. Shrestha SS. Sigdel SR. Traditional uses of medicinal plants in gastrointestinal disorders in Nepal. Journal of Ethnopharmacology. 2014; 158:221-229. doi: 10.1016/j.jep.2014.10.014.
8. Dey P. Bardalai D. Kumar NR. Subramani C. Mukherjee M. Bhakta T. Ethnomedicinal knowledge about various medicinal plants used by the tribes of Tripura. Research Journal of Pharmacognosy and Phytochemistry. 2012; 4(6): 297-302.
9. Mukherjee PK. Harwansh RK. Bahadur S. Banerjee S. Kar A. Evidence-Based Validation of Indian Traditional Medicine: Way Forward. In From Ayurveda To Chinese Medicine. 2017; 2(1):48-61.
10. Jain SK. Standardization & safety measures: Quality based validation of herbal medicine. International Journal Pharmacogn & Chinese Medicine. 2019; 3(4). doi: 10.23880/ipcm-16000182.
11. Baruah D. Choudhury J. Kandimalla R. Kotoky J. A study on the traditional practices of some herbal medicine in the rural health care system of Assam. Punarnav. 2014; 2(3):01-10.
12. Tayung K. Sarkar M. Baruah P. Endophytic fungi occurring in Ipomoea carnea tissues and their antimicrobial potentials. Brazilian Archives of Biology and Technology. 2012; 55(5):653-660. doi: 10.1590/S1516-89132012000500003
13. Thankaraj SR. Sekar V. Kumaradhass HG. Perumal1 N. Hudson AS. Exploring the antimicrobial properties of seaweeds against Plasmopara viticola (Berk. and M.A. Curtis) Berl. and De Toni and Uncinulanecator (Schwein) Burrill causing downy mildew and powdery mildew of grapes. Indian Phytopathology. 2020; 73:185-201. doi:10.1007/S42360-019-00137-6.
14. Kelber O. Bauer R. Kubelka W. Phytotherapy in functional gastrointestinal disorders. Digestive Diseases. 2017; 35(S1):36-42. doi: 10.1159/000485489.
15. Kagyung R. Gajurel PR. Rethy P. Singh B. Ethnomedicinal plants used for gastro-intestinal diseases by Adi tribes of Dehang- Debang biosphere reserve in Arunachal Pradesh. Indian Journal of Traditional Knowledge. 2010; 9(3):496-501.
16. Qureshi MS. Reddy AV. Kumar GS. Nousheen L. Ethnobotanical study on medicinal plants used by traditional health practitioners and villagers of Garhphulghar Gram, Chhattisgarh, India. Asian Journal of Pharmaceutical Research. 2017; 7(2):98-105. doi: 10.5958/2231-5691.2017.00017.X.
17. Patil KB. Patil NB. Patil AA. Medicinal Plants for cure Common Diseases in Shirpur (Maharashtra, India). Asian Journal of Research in Pharmaceutical Science. 2019; 9(4):273-275.
18. Nimbekar T. Wanjari B. Nema M. Bhiskute SM. Traditional Knowledge on Antimicrobial activity of Some Ethnomedicinal Plants used by Tribes of Gondia District in Maharashtra State. Research Journal of Pharmacognosy and Phytochemistry. 2012; 4(3):147-51. doi: 10.36348/sjmps.2020.v06i06.002.
19. Biswakarma S. Pala NA. Shukla G. Chakravarty S. Ethnomedicinal plants used to cure stomach disorders in forest fringe communities in northern part of West Bengal. Indian Journal of Natural Product and Resource. 2017; 8(4):370-380.
20. Siddalinga MS, Vidyasagar GM. Medicinal plants used in the treatment of gastrointestinal disorders in Bellary district, Karnataka. India. Indian Journal of Traditional Knowledge. 2013;12(2):321-325.
21. Vineeta PNA. Shukla G. Chakravarty S. Traditionally used medicinal plants for treatment of stomach disorder in West Bengal, India: A scrutiny and analysis from secondary literature. Studies on Ethnomedicine. 2018; 12(3):163-183. doi: 10.31901/24566772.2018/12.03.530.
22. Pietrovski EF. Rosa KA. Facundo VA. Rios K. Marques MCA. Santos AR. Antinociceptive properties of the ethanolic extract and of the triterpene 3β,6β,16β-trihidroxilup20(29)-ene obtained from flowers of Combretum leprosum in mice. Pharmacology Biochemistry and Behavior. 2006; 83:90–99. doi: 10.1016/j.pbb.2005.12.010.
23. Asuzu IU. Njoku JC. The pharmacological properties of the ethanolic root extract of Combretum dolichopetalum. Phytotherapy Research. 1992; 6:125–128. doi: https://doi.org/10.1002/ptr.2650060304.
24. Naidu N. Kumar GS. Sivakrishna K. Anjinaik K. Kumar LP. Sneha G. Anti microbial and antioxidant evolution of aqueous extract of Terminalia chebula using disc diffusion, H2O2 scavenging methods. Asian Journal of Research in Pharmaceutical Science. 2017; 7(2): 112-114. doi: 10.5958/2231-5659.2017.00017.0.
25. Das B. Dash S. Choudhury RC. Pharmaceutical Properties of Terminalia bellerica (Bahera)-An Overview. Research Journal of Pharmacy and Technology. 2014; 7(5):592-597.
26. Mehra R. Makhija R. Vyas N. Role of Terminalia chebula on Gastrointestinal Mucosa. Research Journal of Pharmacy and Technology. 2012; 5(9):1183-1186.
27. Arun P. Purushotham KG. Jayarani J. Kumari V. Chamundeeswari D. Screening Antibacterial Activity of Various Extracts of Lawsonia inermis. Research Journal of Pharmacognosy and Phytochemistry. 2010; 2(3):185-187.
28. Kaur J. Kaur R. Nagpal AK. Documentation of traditional knowledge on medicinal plants used by local population of Kapurthala, Punjab (India). Journal of Chemical and Pharmaceutical Research. 2017; 9(3):351-355.
29. Okullo JBL. Omujal F. Bigirimana C. Isubikalu P. Malinga M. Bizuru E. Namutebi A. Obaa BB. Agea JG. Ethno medicinal uses of selected indigenous fruit trees from the lake Victoria Basin districts in Uganda. Journal of Medicinal Plants Studies. 2014; 2(1):78-88.
30. Maroyi A. Traditional use of medicinal plants in south-central Zimbabwe: Review and perspective. Journal of Ethnobiology and Ethnomedicine. 2013; 9:31. doi: 10.1186/1746-4269-9-31.
31. Ibrahim N. Kebede A. In vitro antibacterial activities of methanol and aqueous leave extracts of selected medicinal plants against human pathogenic bacteria. Saudi Journal of Biological Sciences. 2020; 27(9):2261-2268. doi: 10.1016/j.sjbs.2020.06.047.
32. Altemimi A. Lakhssassi N. Baharlouei A. Watson DG. Lightfoot DA. Phytochemicals: Extraction, Isolation, and identification of bioactive compounds from plant extracts. Plants. 2017; 6:42. doi: 10.3390/plants6040042.
33. Parekh J. Jadeja D. Chanda S. Efficacy of aqueous and methanol extracts of some medicinal plants for potential antibacterial activity. Turkish Journal of Biology. 2005; 29:203–210.
34. Mudzengi CP. Murwira A. Tivapasi M. Murungweni C. Burumu JV. Halimani T. Antibacterial activity of aqueous and methanol extracts of selected species used in livestock health management. Pharmaceutical Biology. 2017; 55(1):1054-1060. doi: 10.1080/13880209.2017.1287744.
35. Meena AK. Rao MM. Meena RP. Panda P. Pharmacological and phytochemical evidences for the plants of Wedelia Genus–A Review. Asian Journal of Pharmaceutical Research. 2011; 1(1):7-12.
36. Manandhar S. Luitel S. Dahal RK. In vitro antimicrobial activity of some medicinal plants against human pathogenic bacteria. Journal of Tropical Medicine. 2019; Article ID 1895340.
37. Debalke D. Birhan M. Kinubeh A. Yayeh M. Assessments of antibacterial effects of aqueous-ethanolic extracts of Sida rhombifolia’s aerial part. The Scientific World Journal. 2018. Article ID 8429809.
38. Lee HS. Antimicrobial properties of Turmeric (Curcuma longa L.) Rhizome-derived ar-tumerone and curcumin. Food Science and Biotechnology. 2006; 15(4):559-563.
39. Jankasem M. Wuthi-udomlert M. Gritsanapan W. Antidermatophytic properties of Ar-tumerone, turmeric oil, and Curcuma longa preparations. ISRN Dermatology. 2013; 250597. doi: 10.1155/2013/250597.
40. Negi PS. Jayprakash JK. Rao LJM. Sakariah KK. Antimicrobial activity of turmeric oil- A byproduct from curcumin manufacture. Journal of Agricultural Food Chemical. 1999; 47:2397–2400. doi:10.1021/jf990308d.
41. Jeyarani T. Subramanian S. Sneha R. Sudha ML. Negi PS. Characterisation of mango kernel fat and preparation of trans-free margarine for use in muffins. Journal of Nutrition and Food Science. 2015; 5(3). doi: 10.4172/2155-9600.1000357.
42. Erwa IY. Matenje FS. Mwachumu YM. Ishag OAO. Ahmed HM. Extraction, physicochemical characteristics and fatty acid profile of kernel oil from Mangifera indica L. cultivated in Sudan. Chemical Science International Journal. 2019; 28(4):1-7.
43. Agoramoorthy G. Chandrasekaran M. Venkatesalu V. Hsu MJ. Antibacterial and antifungal activities of fatty acid methyl esters of the blind-your-eye mangrove from India. Brazilian Journal of Microbiology. 2007; 3:739-742. doi: https://doi.org/10.1590/S1517-83822007000400028.
44. Adegoke AS. Jerry OV. Ademola OG. GC-MS Analysis of Phytochemical Constituents in Methanol Extract of Wood Bark from Durio Zibethinus Murr. International Journal of Medicinal Plants and Natural Products. 2019; 5(3):1-11.doi: 10.20431/2454-7999.0503001.
45. Bailey AV, De Lucca AJ. Moreau JP. Antimicrobial properties of some erucic acid- glycolic acid derivatives. Journal of the American Oil Chemists' Society. 1989; 66:932-934.
46. Smith E. Williamson E. Zloh M. Gibbons S. Isopimaric acid from Pinus nigra shows activity against multidrug resistant and EMRSA strains of Staphylococcus aureus. Phytotherapy Research. 2005; 19(6):538-542. doi: 10.1002/ptr.1711.
47. Gnanasekaran T. Vavitsas K. Ranberg JA. Nielsen AZ. Olsen CE. Hamberger B. Jensen PE. Heterologous expression of the isopimaric acid pathway in Nicotiana benthamiana and the effect of N-terminal modifications of the involved cytochrome P450 enzyme. Journal of Biological Engineering. 2015; 9:24. doi: 10.1186/s13036-015-0022-z.
48. Yuan H. Ma Q. Ye L. Piao G. The traditional medicine and modern medicine from natural products. Molecules. 2016; 21:559. doi: 10.3390/molecules21050559.