Author(s):
Raja Chakraverty, Chowdhury Mobaswar Hossain, Anjan Adhikari, Pranabesh Chakraborty
Email(s):
rchakraborty20@yahoo.com
DOI:
10.52711/0974-360X.2023.00397
Address:
Raja Chakraverty1*, Chowdhury Mobaswar Hossain2, Anjan Adhikari3, Pranabesh Chakraborty4
1Doctoral Scholar (Pharmaceutical Technology), Maulana Abul Kalam Azad University of Technology, Nadia - 741249, West Bengal.
2 Head, Department of Pharmaceutical Technology, Maulana Abul Kalam Azad University of Technology, Haringhata, Nadia - 741249, West Bengal.
4Director, School of Food, Pharmaceutical, Medical Science and Technology, Makaut, Haringhata, Nadia - 741249, West Bengal.
3Head, Department of Pharmacology, MJN Medical College and Hospital, Coochbehar, West Bengal.
*Corresponding Author
Published In:
Volume - 16,
Issue - 5,
Year - 2023
ABSTRACT:
The herb Neptunia prostrata Linn. Is belonging to the family Mimosaceae have been used in folkloric medicine in the North-eastern states of Indiaof Assam and Tripura by indigenous herbal healers since time immemorial but there is a scarcity of any background study documenting its use as an antimicrobial herb. For the same, plants were collected and authenticated. Following identification of these herbs methanolic, ethanolic, pet ether and chloroform extracts were prepared using soxhletion. Acute toxicity study as per OECD guidelines 420 was assessed in wistar albino rats and in swiss albino mice (n=5) of both sexes at doses of 2000mg/kg body weight and did not reveal any morbidity or mortality in the animals within the stipulated period.Phytochemical screening was performed on all four extracts of Neptunia prostrata. Phytochemical constituents depicted presence of glycoside, flavonoids in only ethanolic, methanolic and chloroform extracts. Alkaloids were present in the chloroform extract. The antimicrobial activity was performed by disc agar diffusion method with respect to amoxicillin at standard doses against ATCC strains of Gram positive Staphylococcus aureus, Bacillus subtilis and Gram negative Salmonella typhi. The herbs showed antioxidant activity comparable to standard antioxidants in-vitro such as Ascorbic acid (Vitamin C) with comparable IC 50 values. Results of the antibacterial assay on the basis of zone of inhibition (mm) and MIC values of extracts of the extract (NPHE) under study to suggest these two indigenous herb have conspicuous and potent putative role in the therapeutics of a vast plethora bacterial infections that need to be corroborated for the expansion of future prospective in-vivo studies with larger sample size.
Cite this article:
Raja Chakraverty, Chowdhury Mobaswar Hossain, Anjan Adhikari, Pranabesh Chakraborty. Phytochemical Characterisation, Antioxidant and Antidiabetic activity of extracts of Neptunia prostrata Linn. Research Journal of Pharmacy and Technology 2023; 16(5):2411-4. doi: 10.52711/0974-360X.2023.00397
Cite(Electronic):
Raja Chakraverty, Chowdhury Mobaswar Hossain, Anjan Adhikari, Pranabesh Chakraborty. Phytochemical Characterisation, Antioxidant and Antidiabetic activity of extracts of Neptunia prostrata Linn. Research Journal of Pharmacy and Technology 2023; 16(5):2411-4. doi: 10.52711/0974-360X.2023.00397 Available on: https://rjptonline.org/AbstractView.aspx?PID=2023-16-5-56
REFERENCE:
1. Adebolu, T.T. and Oladimeji, S.A.. Antimicrobial activity of leaf extracts of Ocimumgratissium on selected diarrhoea causing bacteria in southwestern Nigeria. Afr. J. Biotechnol. 2005, 4: 682 - 684: 10.5958/2231-5713.2020.00011.2
2. Adiguzel, A., Gulluce, M., Sengul, M., Ogutcu, H., Sahin, F. and Karaman, I. Antimicrobial effects of Ocimumbasilicum (Labiatae) extract. Turk J. Biol. 2005, 29: 155 - 160: 10.52711/0974-360X.2022.00739
3. Ates, D.A. and Erdogrul, O.T. Antimicrobial activity of various medicinal and commercial plants extracts. Turk J. Biol. 2003, 27: 157 – 162:10.5958/2231-5713.2020.00011.2
4. Burt, S.A.. Essential oils: their antibacterial properties and potential applications in foods- a review. Int. J. Food Microbiology. 2004; 94: 223 – 253: 10.52711/0974-360X.2322.40738
5. Burt, S.A. and Reinders, R.D. Antimicrobial activity of selected plant essential oils against Escherichia coli O157:H7. Lett. Appl. Microbiol. 2003; 36: 162 – 167:10.5958/2231-5713.2020.00012.4
6. Chiej, R. (1984). Encyclopaedia of Medicinal Plants. MacDonald.
ISBN 0-356-10541-5, Little, Brown.
7. Chopra, R.N., Nayar, S.L. and Chopra, I.C. (1986). Glossary of Indian Medicinal Plants (Including the Supplement). Council of Scientific and Industrial Research. New Delhi.
8. Davis, J. Inactivation of antibiotics and the dissemination of resistance genes. Science, 2004; 264: 375 - 382:10.5958/2231-5713.2020.00011.4
9. Duke, J.A. and Ayensu, E.S. Medicinal Plants of China. Reference Publications. Inc.: ISBN 0-917256-20-4. 1985; Reference Publications, Algonac, MI (USA).
10. Dulger, B. and Gonuz, A. Antimicrobial activity of some endemic Verbascum, Salvia, and Stachy species. Pharm. Biol. 2004; 42: 301 - 304:10.5958/2231-5714.2020.00011.8
11. Dulger, B. and Gonuz, A. Antimicrobial activity of certain plants used in Turkish traditional medicine. Asian J. Plant Sci. 2004; 3: 104 – 107:10.5958/2231-5713.2020.00011.6
12. Erturk, O., Kati, H., Yayli, N. and Demirbag, Z.. Antimicrobial activity of Viscum album L. subsp. Abietis (Wiesb). Turk J. Biol., 2003; 27: 255 – 258:10.52711/0974-360X.2022.00745
13. Falerio, M.L., Miguel, M.G., Laderio, F., Venâncio, F., Tavares, R., Brito, J.C., Figueiredo, A.C., Barroso, J.G. and Pedro, L.G.. Antimicrobial activity of essential oils isolated from Portuguese endemic species of Thymus. Lett. Appl. Microbiol. 2003; 36: 35 - 40
14. Hayat, M.A. (1981). Principles and techniques of electron microscopy, Vol. 1. Edward Arlond Lt., London, 522 p.
15. Izzo, A.A., Di Carlo, G., Bicardi, D., De Fusco, R., Mascolo, N., Borrelli, F. and Capasso, F.. Biological Screening of Italian Medicinal Plants for Antibacterial Activity. Phytotherapy Res.2015; 9: 281 - 286.
16. Jirovetz, L., Buchbauer, G., Shafi, M.P. and Kaniampady, M.M..Chemotaxonomical analysis of the essential oil aroma compounds of four different Ocimum species from southern India. Eur. Food Res. Technol. 2013; 217: 120–124:10.52711/0974-224.2022.00287
17. Juliani, H.R. and Simon, J.E.. Antioxidant activity of basil. In: Trends in new crops and new uses. (Janick J and Whipkey A eds.), ASHS Press, Alexandria, V A, 2002; pp. 575 - 579: 10.52711/0974-224.2022.00499
18. Kalemba, D. and Kunicka, A. Antimicrobial and antifungal properties of essential oils. Curr. Med. Chem. 2003; 10: 813 – 829: 10.58712/0974-224.2022.00459
19. Kivcak, B., Mert, T. and Ozturk, H.T.. Antimicrobial and cytotoxic activity of Ceratonia siliqua L. extracts. Turk J. Biol.2002; 26: 197 - 200.
20. Lusture, J. (1983). The Herb Book. Bantam books: ISBN 0-553-23827-2, New York.
21. Martin, K.W. and Ernst, E.. Herbal medicines for treatment of fungal infections: a systematic review of controlled clinical trials. Mycoses. 2004; 47: 87 - 92
22. Ncube NS, Afolayan AJ, Okoh AI. Assessment techniques of antimicrobial properties of natural compounds of plant origin: current methods and future trends. African Journal of Biotechnology 2008; 7 (12): 1797-1806.
23. Handa SS, Khanuja SPS, Longo G, Rakesh DD. Extraction Technologies for Medicinal and Aromatic Plants. International centre for science and high technology. Trieste. 2008, 21-25.
24. Das K, Tiwari RKS, Shrivastava DK. Techniques for evaluation of medicinal plant products as antimicrobial agent: Current methods and future trends. Journal of Medicinal Plants Research. 2010; 4(2): 104-111.
25. Parekh J, Karathia N, Chanda S. Evaluation of antibacterial activity and phytochemical analysis of Bauhinia variegataL. bark. African Journal ofBiomedical Research. 2006; 9: 53-56.
26. Eloff JN. Which extractant should be used for the screening and isolation of antimicrobial components fromplants. Journal of Ethnopharmacology. 1998; 60: 18.
27. KP SG, CM M, Kumar V. Study on the diuretic activity of Cynodondactylon root stalk extract in albino rats. Research Journal of Pharmacy and Technology. 2009;2(2):338-40.
28. Shaikh S, Dubey R, Dhande S, Joshi YM, Kadam VJ. Phytochemical and pharmacological profile of Aphanamixispolystachya: An overview. Research Journal of Pharmacy and Technology. 2012;5(10):1260-3.
29. Tripathi S, Chaurey M, Balasubramaniam A, Balakrishnan N. Grewia asiatica Linn.-As a Phytomedicine: A Review. Research Journal of Pharmacy and Technology. 2010;3(1):1-3.
30. Basavaraj H, Purnima A. In–vitro antioxidant activity of aqueous and ethanolic extract of Cosciniumfenestratum root and Embeliaribes flower. Research Journal of Pharmacy and Technology. 2012;5(4):513-7.
31. Manokaran S, Jaswanth A, Sengottuvelu S, Nandhakumar J, Duraisamy R, Karthikeyan D, Mallegaswari R. Hepatoprotective activity of Aervalanata Linn. against paracetamol induced hepatotoxicity in rats. Research Journal of Pharmacy and Technology. 2008; 1(4):398-400.
32. Ekka NR, Namdeo KP, Samal PK. Standardization strategies for herbal drugs-An overview. Research Journal of Pharmacy and Technology. 2008 Dec 28;1(4):310-2.10.52711/0974-224.2022.00245
33. Singh A, Gowri K, Chitra V. A Review on Phytochemical Constituents and Pharmacological Activities of the plant: Aervalanata. Research Journal of Pharmacy and Technology. 2020 Mar 1;13(3):1580-6:10.52711/0974-224.2022.00566
34. Ghaisas MM, Wadikar AD, Gulati TB, Limaye RP. Anxiolytic Effect of a Methanolic Extract of the EmbeliaribesBurm F. in Mice. Research Journal of Pharmacy and Technology. 2010; 3(4):1136-9:10.52711/0974-224.2022.00499
35. Dubey SK, Batra A. Anti diabetic activity of Thuja occidentalis Linn. Research Journal of Pharmacy and Technology. 2008;1(4):362-5:10.52711/0974-369.2022.00744
36. Nayak A, Sajini RJ, Padmavati D, Karthik R. Chemistry and medicinal properties of Cosciniumfenestratum (Gaertn.) Colebr (Tree Turmeric). Research Journal of Pharmacy and Technology. 2012;5(2):198-202:10.52711/0974-36566.2022.00564
37. Jain A, Ojha V, Kumar G, Karthik L, Rao KV. Phytochemical composition and antioxidant activity of methanolic extract of Ficus benjamina (moraceae) leaves. Research Journal of Pharmacy and Technology. 2013; 6(11):1184-9: 10.52711/0974-560.2022.00736.
38. Choudhary N, Prabhu KS, Prasad SB, Singh A, Agarhari UC, Suttee A. Phytochemistry and Pharmacological exploration of Chenopodium album: Current and future perspectives. Research Journal of Pharmacy and Technology. 2020 12;13(8):3933-40.10.52711/0974-455.2022.00736.
39. Paul SK, Goyal S, Yadav P, Yadav S, Parashari D. Phytochemistry and Pharmacological aspects of Murrayakoenigii Linn. Research Journal of Pharmacy and Technology. 2013;6(6):695-7:: 10.52711/0974-360X.2022.00739.
40. Sureshkumar CA, Meera R, Devi P, Sathish M, Varadharajan R, Muthumani P. Antinociceptive, Anti-inflammatory activity of Coriander sativum Leaves. Research Journal of Pharmacy and Technology. 2010;3(3):744-7.
41. Maroyi A. Pharmacological properties and medicinal applications of Lantana rugosa Thunb. (Family Verbenaceae). Research Journal of Pharmacy and Technology. 2021 Jan 29;14(1):259-64: 10.52711/0974-380.2022.00744
42. Adnyana IK, Sukandar EY. Pharmacological effects of Garcinia mangostana L.: An Update Review. Research Journal of Pharmacy and Technology. 2020 Nov 13;13(11):5471-6.
43. Gunasekaran B, Muralidharan P, Pandiselvi A, Amutha P. Preliminary Phytochemical screening and Anti oxidant activities of Ethanolic extract of Caesalpinia sappan Linn. Research Journal of Pharmacy and Technology. 2008; 1(3):179-81:: 10.52711/0974-560.2022.00736
44. Rakha P, Nagpal M, Sharma S, Parle M. Anti-Inflammatory activity of petroleum ether extract of seeds of OcimumBasilicum Linn. Research Journal of Pharmacy and Technology. 2009; 2(3): 589-91: 10.52711/0974-380X.2022.00776
45. Samal PK, Dangi JS, Meena KP, Beck NR, Maheshwari G, Patel A. Hepatoprotective Activity of Butea monosperma bark on Liver Damage Caused by Paracetamol in Rats. Research Journal of Pharmacy and Technology. 2011; 4(5): 771-4:10.52711/0974-360X.2022.00739