ABSTRACT:
Polycystic Ovarian Syndrome is a heterogenous disorder characterized by hyperandrogenism, chronic anovulation and hyperglycaemia. An attempt is made in the present study to reduce the complications of PCOS especially hyperglycaemia and hyperandrogenism through the ethanolic root extracts of Asparagus racemosus by performing in vitro and in silico experiments. In vitro experiments such as inhibitory activity of a amylase and glucose adsorption, inhibition of glucose diffusion and glucose uptake by yeast cells were performed concentrating the enteric system to reduce the glucose entry into the blood circulation to manage the hyperglycaemia. In silico methods were performed using patch dock to screen the suitable antagonistic phyto ligand from the plant for the IRS1 and IRS 2 receptors for the negative down regulation of androgen production. The selected plant extract exhibited a concentration dependent glucose maintaining activity in the present investigation which was comparable to the positive drug Acarbose which exerts hypoglycemic activity acting as enteric system. In the patch dock analysis five ligands namely Quercetin, Racemosol, Rutin, Hyperoside and Shatavarin I were selected as best antagonistic ligands for the IRS1 and IRS 2 receptors in terms of their docking score and amino acid interactions. It can be concluded that the ethanolic root extracts of Asparagus racemosus can be considered as a potential drug for the management of PCOS through their dual role of reducing hyperglycaemia and hyperandrogenism.