Author(s): Nayan Talukdar, Karabi Das, Kemina Moirangthem, Indrani Barman


DOI: 10.52711/0974-360X.2023.00326   

Address: Nayan Talukdar1, Karabi Das1, Kemina Moirangthem1, Indrani Barman2*
1Program of Biotechnology, Faculty of Science, Assam down town University, Guwahati 781026, Assam, India.
2Program of Biochemistry, Faculty of Science, Assam down town University, Guwahati 781026, Assam, India.
*Corresponding Author

Published In:   Volume - 16,      Issue - 4,     Year - 2023

Allium tuberosom, a species native to Chinese province of Shanxi and also found in the North-eastern states of India especially Manipur, is well known for its medicinal properties such as antibacterial, cardiac, stimulant, anti-cancer, etc. Fresh samples of Allium tuberosum were collected from Manipur and assayed for the presence of potent phytochemicals. Moreover, the extracts of the sample were also investigated for their anti-oxidant and anti-diabetic activities. Two solvents of varied polarity were used for soxhlet extraction and standard protocols were employed throughout the study. Presence of various phytochemicals such as alkaloids, flavonoids, saponins, etc. was reported. Upon evaluation of anti-oxidant activity by DPPH method, it was recorded that more the concentration of the sample more was its anti-oxidant activity for both the extracts (hexane and methanol), i.e. upto 63.1% for hexane extract and 67.4% for methanol extract. Anti-diabetic activity by a-amylase inhibition assay was also dependent on its concentration. 64.19% for hexane extract and 62.7% for methanol extract of a-amylase inhibiting activity was recorded. The results of the current study prove the potentiality of the species to be further considered as a valuable asset for clinical trials so as to be explored in pharmaceutical purposes.

Cite this article:
Nayan Talukdar, Karabi Das, Kemina Moirangthem, Indrani Barman. Evaluation of In-vitro Anti-oxidant and α-amylase inhibition activity of Allium tuberosom and its Phytochemical analysis. Research Journal of Pharmacy and Technology 2023; 16(4):1987-1. doi: 10.52711/0974-360X.2023.00326

Nayan Talukdar, Karabi Das, Kemina Moirangthem, Indrani Barman. Evaluation of In-vitro Anti-oxidant and α-amylase inhibition activity of Allium tuberosom and its Phytochemical analysis. Research Journal of Pharmacy and Technology 2023; 16(4):1987-1. doi: 10.52711/0974-360X.2023.00326   Available on:

1.    Saini N, Wadhwa S, Singh GK, Rawal S, Singhal S. Comparative study between cultivated garlic (Allium sativum) and wild garlic (Allium tuberosum). Asian Journal of Pharmaceutical Research and Development. 2013 Jul 1:108-25.
2.    Panda S, Mandal M, Satpathy M. Evaluation of the extract of Allium cepa Linn. for biochemical and antibacterial activities. Asian Journal of Research in Chemistry. 2016;9(3):113-5.10.5958/0974-4150.2016.00020.1
3.    Khalid N, Ahmed I, Latif MS, Rafique T, Fawad SA. Comparison of antimicrobial activity, phytochemical profile and minerals composition of garlic Allium sativum and Allium tuberosum. Journal of the Korean Society for Applied Biological Chemistry. 2014 Jun;57(3):311-7.
4.    Roshan S, Khan A, Ali S, Tazneem B. Effect of Allium sativum in Immoblilization Stress Induced Albino Rats. Liver. 2010 Aug 28;5(62.5):6-818.
5.    Choudhary R. Benificial effect of Allium sativum and Allium tuberosum on experimental hyperlipidemia and atherosclerosis. Pakistan Journal of Physiology. 2008 Dec 31;4(2):7-10.
6.    Sultana F, Mohsin M, Sah AN. In-vitro Antioxidant and Antimicrobial Activity of Allium tuberosum Rottler. ex Spreng. Int. J. Adv. Res. Biol. Sci. 2015;2(12):178-87.
7.    Savithramma N, Rao ML, Suhrulatha D. Screening of medicinal plants for secondary metabolites. Middle-East Journal of Scientific Research. 2011;8(3):579-84.
8.    Karthik VP, Suresh P, David DC. In vitro hydrogen peroxide scavenging activity and alpha amylase inhibitory activity of Croton tiglium extract. Research Journal of Pharmacy and Technology. 2019 Jun 1;12(6):3045-7. DOI: 10.5958/0974-360X.2019.00515.8
9.    Gogoi P, Islam M. Phytochemical screening of Solanum nigrum L and S. myriacanthus Dunal from districts of upper Assam, India. IOSR J Pharm. 2012;2(3):455-9. 10.9790/3013-0230455459
10.    Banu KS, Cathrine L. General techniques involved in phytochemical analysis. International journal of advanced research in chemical science. 2015 Apr;2(4):25-32.
11.    Jose BE, Panneerselvam P. In vitro Antidiabetic activity of Ethanol and Aqueous leaf extracts of Azima tetracantha Lam. Research Journal of Science and Technology. 2019 Mar 31;11(1):64-8. 10.5958/2349-2988.2019.00009.3
12.    Singh MP, Jaiswal R, Mandal S. Evaluation of Immunomodulatory Activity of Aqueous Extract of the Bulb of Allium sativam In Wistar Rats. Research Journal of Pharmacology and Pharmacodynamics. 2010;2(6):401-6.
13.    Molyneux P. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J. sci. technol. 2004 Dec;26(2):211-9.
14.    SLDV RM, Das MC, Vijayaraghavan R, Shanmukha I. In vitro evaluation of antidiabetic activity of aqueous and ethanolic leaves extracts of Chloroxylon swietenia. National Journal of Physiology, Pharmacy and Pharmacology. 2017;7(5):486.
15.    Bimal KG, Eun SS, Eun HK, Amal KG, Chang YY, Bal KG. A comparative evaluation of the antioxidant activity of some medicinal plants popularly used in Nepal. Journal of Medicinal Plants Research. 2011 May 18;5(10):1884-91.
16.    Nhut PT, An TN, Minh LV, Truc TT, Anh NH. Phytochemical screening of Allium Tuberosum Rottler. ex Spreng as food spice. InIOP Conference Series: Materials Science and Engineering 2020 Dec 1 (Vol. 991, No. 1, p. 012021). IOP Publishing.
17.    Manimaran M, Kannabiran K. Alpha amylase activity of Streptomyces sp. VITMK1 isolated from marine soil sample of Pichavaram, Tamil Nadu, India. Research Journal of Pharmacy and Technology. 2018 Jun 30;11(6):2180-2. 10.5958/0974-360X.2018.00403.1
18.    Helen PA, Bency BJ. Inhibitory potential of Amaranthus viridis on α-amylase and glucose entrapment efficacy In vitro. Research Journal of Pharmacy and Technology. 2019;12(5):2089-92.
19.    Khanam Z, Wen CS, Bhat IU. Phytochemical screening and antimicrobial activity of root and stem extracts of wild Eurycoma longifolia Jack (Tongkat Ali). Journal of King Saud University-Science. 2015 Jan 1;27(1):23-30. 10.1016/j.jksus.2014.04.006
20.    Rangasamy P, Hansiya VS, Maheswari PU, Suman T, Geetha N. Phytochemical analysis and evaluation of In vitro antioxidant and anti-urolithiatic potential of various fractions of Clitoria ternatea L. Blue flowered leaves. Asian Journal of Pharmaceutical Analysis. 2019 Apr;9(2):67-76. 10.5958/2231-5675.2019.00014.0
21.    Talukdar N, Dutta AM, Chakraborty R, Das K. Screening of phytochemicals, antioxidant and inhibitory effect on alpha-amylase by ethanolic extract of Elaeocarpus Ganitrus (Bark). International Journal of Pharmaceutical Sciences and Research. 2017 Dec 1;8(12):5270-5.10.13040/IJPSR.0975-8232.8(12).5270-75
22.    Kasote DM, Katyare SS, Hegde MV, Bae H. Significance of antioxidant potential of plants and its relevance to therapeutic applications. International journal of biological sciences. 2015;11(8):982. 0.7150/ijbs.12096
23.    Narasimhan R. Phytochemical screening and antioxidant studies in the Pulp extracts of Cucurbita maxima. Asian Journal of Pharmaceutical Research. 2016 Mar 1;6(1). 10.5958/2231-5691.2016.00001.0  

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