Kowsalya J, Kumudhaveni B, Jiyavutheen M, Kavithasai M, Radha R
Kowsalya J*, Kumudhaveni B, Jiyavutheen M, Kavithasai M, Radha R
Department of Pharmacognosy, College of Pharmacy, Madras Medical College, Chennai, Tamilnadu, India.
Volume - 16,
Issue - 4,
Year - 2023
Barleria cristata Linn. belongs to the family Acanthaceae, commonly known as Phillipine Violet. It has been traditionally used in the treatment of diseases including anaemia, toothache, respiratory disorders, skin diseases and cardio-vascular disorders. The aim of this study was intended to evaluate the in vivo cardio-protective activity of ethanolic extract of the leaves of Barleria cristata Linn. using Daphnia magna as the model. Pharmacognostical characters viz., macroscopy, microscopy, linear measurements, physicochemical constants, loss on drying, fluorescence analysis, determination of heavy metals and phytochemical studies were performed for the leaves of Barleria cristata. Acute toxicity study was performed as per OECD guidelines 202 which revealed that no significant mortality was observed up to 6µg/ml of ethanolic extracts of leaves of Barleria cristata Linn. The ethanolic extract of Barleria cristata with a dose of 20, 40, 60, 80 and 100µg/ml were tested on lactose induced arrhythmia of Daphnia magna and compared with standard metoprolol. The cardio protective activity of ethanolic extracts of Barleria cristata Linn. were observed separately in the Daphnia magna of control, lactose induced, treated with metoprolol and ethanolic extract groups. The results showed that the ethanolic extract of the plant has dose dependent cardio-protective activity on Daphnia magna. Pharmacognostical characters of Barleria cristata are used to identify and standardize the plant and there was no heavy metal toxicity was observed from the leaves of Barleria cristata Linn.
Cite this article:
Kowsalya J, Kumudhaveni B, Jiyavutheen M, Kavithasai M, Radha R. Assessment of Cardio-protective activity of Barleria cristata Linn. using Daphnia magna as model. Research Journal of Pharmacy and Technology 2023; 16(4):1587-2. doi: 10.52711/0974-360X.2023.00259
Kowsalya J, Kumudhaveni B, Jiyavutheen M, Kavithasai M, Radha R. Assessment of Cardio-protective activity of Barleria cristata Linn. using Daphnia magna as model. Research Journal of Pharmacy and Technology 2023; 16(4):1587-2. doi: 10.52711/0974-360X.2023.00259 Available on: https://rjptonline.org/AbstractView.aspx?PID=2023-16-4-7
1. Introduction and importance of medicinal plants and herbs [online]. 2019 [cited 2019 Jan 12]; Available from: URL: https://www.nph.gov.in/introduction-and-importance-of-medicinal-plants-and-herbs mt1.
2. Safety issues in the preparation of homeopathic medicines [online]. 2019 [cited 2019 Jan 21]; Available from: URL: http://www.who.int/medicine/areas/traditional/select mono vol4.pdf
3. Cardiac arrhythmia- A brief introduction. Kastur JA. University of Maryland, Baltimore, Maryland, USA.
4. Sharma HL and Sharma KK. Principles of pharmacology. 2nd ed. Delhi: Paras Medical Publisher: 2017; 292-310.
5. Yashashree VJ. Ancient literature and analysis of Ayurvedic medicine-manuscripts. 2018 Dec;7(3).
6. Kumudhaveni B et al. A Review on phytochemical and pharmacological activity of medicinal plant Barleria cristata. Res J Pharm Tech2020 Feb;13(2):999-1003.
7. Barleria cristata: Perspective towards phytopharmacological aspects [online]. 2019 [cited 2019 Feb 22]; Available from: URL: https://online library.wiley.com. Ethano medicinal uses of Barleria cristata/doi/full/ 10.1111 / jphp.12881.
8. Edward FG, Linda L. Barleria cristata fact sheet FPS-60, Environmental Horticulture Department, Institute of Food and Agricultural Sciences, University of Florida; Oct 1999.
9. Shanaz Banu, Josephine Leno Jenita, Manjula. D, K. B. Premakumari. Pharmacognostical Studies and Isolation of an alkaloid from Barleria cristata Linn. roots. Research J. Pharm. and Tech. 2020; 13(1):163-167.Inamdar JA et al. Structure and development of stomata in Acanthaceae family. Proc Indian Acad Sci. 2006;92(3).
10. Wallis TE. Textbook of Pharmacognosy. 5th ed. Delhi: CBS publishers and Distributors, Shahdara; 1985.
11. Trease GE, Evans WC. The textbook of Pharmacognosy. 16th ed. Edinburgh: Saunders Elseiver; 1978.
12. Wallis TE. Textbook of Pharmacognosy. 4th ed. Delhi: CBS publishers and Distributors, Shahdara; 1962.
13. Trease GE, Evans WC. The textbook of Pharmacognosy.10th ed. Edinburgh: Saunders Elseiver; 1961.
14. Trease GE, Evans WC. The textbook of Pharmacognosy. 11th ed. Edinburgh: Saunders Elseiver; 1978.
15. Paliwal GS. Structure and ontogeny of stomata in some Acanthaceae, Phytomorphology. 1966; 527-32.
16. Kokate CK. Practical Pharmacognosy. 4th ed. Delhi: Vallabh Prakashan publications; 1998.
17. British Pharmacopoeia Commission. British Pharmacopoeia 2016. London: TSO; 2016.
18. Brijesh kumar. WHO guidelines for Herbal Drugs; Part 3. 2011;3(4).
19. Reema Dheer, Surendra Kumar Swarnkar, Farah Syeed. Chromatographic Analysis of Barleria prionitis Linn. Research J. Pharm. and Tech 2019; 12(8):3679-3686.
20. Mawla AM et al. Isolation of phenylethanoid glycosides from Barleria cristata Linn. callus culture. BulI Pharm Sci Assuit Univ. 2015 Dec;28(2):199-204.
21. Mukul Pet al. Comparative analysis of phytochemical, cytotoxic anti-oxidant activity in leaf and bark extract of Barleria cristata. European J Pharm Med Res, 2015;2(3):586-93.
22. Chase CR, Pratt RJ. ‘Fluorescence of powdered vegetable drugs with a particular reference to development of Association’; 1978; vol 38:32-7.
23. Baskar S et al. Phytochemical, trace metal assessment and microbial efficiency of Barleria cristata. Int J Pharm Res, 2015;5(10).
24. Harborne JB. Phytochemical methods: A guide to modern techniques of plant analysis. 2nd ed. Chapman and Hall, London New York; 1983.
25. Mukurjee K Pulok. Quality and evaluation of herbal drugs. Elseiver; 2002; chapter 4:99-104.
26. K Amutha , D Victor Arokia Doss. In Vitro Antioxidant Activity of Ethanolic Extract of Barleria cristata L. Leaves. Research J. Pharmacognosy and Phytochemistry 2009; 1(3): 209-212.
27. Kokati Vankata Bhaskara Rao, Minki Munjal, Amie Patnayak, Loganathan Karthik, Gaurav Kumar. Phytochemical Composition, Antioxidant, Antimicrobial and Cytotoxic Potential of Methanolic Extracts of Adhatoda vasica (Acanthaceae). Research J. Pharm. and Tech. 6(9): September 2013; Page 1004-1009.
28. Shabi Ruskin, R., S. Ajina. Qualitative Phytochemical Screening and In-vitro Anthelmintic Activity of Adhatoda vasica (Acanthaceae). Research J. Pharm. and Tech. 2017; 10(2): 414-420.
29. Ekta Singh Chauhan, Kriti Sharma, Renu Bist. Andrographis paniculata: A Review of its Phytochemistry and Pharmacological Activities. Research J. Pharm. and Tech 2019; 12(2):891-900.
30. Firoj A. Tamboli, Harinath N. More. In vitro antipsoriatic study of successive solvent extracts of Barleria gibsoni Dalz. using HaCa T keratinocyte cells. Research J. Pharm. and Tech. 8(11): Nov., 2015; Page 1566-1569.
31. Akash Mazumdar, Neelam Gurung, Sumit Phulera, Jinsu Mathew, Gaurav Kumar, Loganathan Karthik, Kokati Venkata Bhaskara Rao. Evaluation of Antimicrobial Activity of Ipomoea fistulosa, Furcreae foetida and Barleria mysorensis: An in vitro study. Research J. Pharm. and Tech. 5(8): August 2012; Page 1081-1084.
32. S. Hemmalakshmi, S. Annapurani, S. Gayathri Devi. Comparative Phytochemical Screening and Total Phenolic Content of Different Extracts of Ficus racemosa, Morinda Tinctoria and Nerium Indicum Fresh Leaves. Research J. Pharm. and Tech 2016; 9(12):2222-2227.
33. S. Khadeerunnisa, S. Nelson Kumar, C. Rajaram, R. Manohar, K. Ravindra Reddy. Evaluation of Antiulcer Activity of Methanolic Extract of Barleria buxifolia in Experimental Rats. Research J. Pharm. and Tech 2020; 13(2):533-537.
34. OECD. Guidance document on acute oral toxicity. Experimental health and Safety Monograph Series on Testing and Assessment no.24; 2000.
35. Narmadha R, Devaki K. Toxicological evaluation and glucose tolerance test of ethanolic extract of Barleria cristata Linn. in Wistar albino rats, 2013.
36. S. Sheeba Tabassum, C. Rajaram1, S. Nelson Kumar, R. Manohar, K. Ravindra Reddy. Evaluation of Hepatoprotective activity of the Methanolic Extract of Barleria Cuspidata against CCl4 Induced Liver damage in Experimental Rats. Research J. Pharm. and Tech 2020; 13(2):538-542.
37. Jinsu Mathew, Kavita Maria Arora, Akash Mazumdar, Gaurav Kumar, Loganathan Karthik, Kokati Venkata Bhaskara Rao. Evaluation of phytochemical composition and antioxidant activity of aqueous extract of Barleria mysorensis and Furcraea foetida leaves. Research J. Pharm. and Tech. 5(12): Dec. 2012; Page 1503-1508.
38. Shah R. ‘Daphnia as an emerging model for toxicological genomics, its ecology and evolution. Adv Exp Biol; 2004; vol.2.
39. Periyanayagam K, Karthikeyan V. Cardioprotective effect of the leaves of Artocarpus heterophyllus L. on Daphnia magna. Innovare J Health Sci; 2013; vol.1(3):1-5.