Author(s):
Nur Vita Purwaningsih, Ellies Tunjung Sari Maulidyanti, Rahma Widyastuti
Email(s):
elliestunjungismail@gmail.com
DOI:
10.52711/0974-360X.2023.00309
Address:
Nur Vita Purwaningsih, Ellies Tunjung Sari Maulidyanti*, Rahma Widyastuti
Department of Medical Laboratory Technology, Faculty of Health Sciences, Muhammadiyah University of Surabaya, Surabaya 60113, Indonesia.
*Corresponding Author
Published In:
Volume - 16,
Issue - 4,
Year - 2023
ABSTRACT:
The selection of tools, especially the use of vacutainer tubes, affects the results of the examination to determine the stability of serum from plain vacutainer with vacutainer separation gel on the quality of uric acid and glucose test results. This study used an experimental design. The study took blood samples of 3 ml each in SST vacutainer and plain vacutainer serum. The mean uric acid levels using plain vacutainer and SST vacutainer were 4.5875±0.59048 and 4.9250±0.56318, respectively. The mean glucose levels using plain vacutainer and SST vacutainer were 90.64±12.33 and 94.12±13.30, respectively. Uric acid and glucose levels using gel-separated vacutainer were higher than uric acid levels using plain vacutainer. There was a significant difference in uric acid levels between regular vacutainers and SST vacutainers, similar to glucose levels (p = 0.000, p = 0.000). Uric acid and glucose levels using SST vacutainer were higher than uric acid levels using plain vacutainer. The following types of vacutainer separator gel and plain vacutainer affect the quality of uric acid and glucose test results.
Cite this article:
Nur Vita Purwaningsih, Ellies Tunjung Sari Maulidyanti, Rahma Widyastuti. Comparison of Serum Stability against Uric Acid and Glucose. Research Journal of Pharmacy and Technology 2023; 16(4):1885-9. doi: 10.52711/0974-360X.2023.00309
Cite(Electronic):
Nur Vita Purwaningsih, Ellies Tunjung Sari Maulidyanti, Rahma Widyastuti. Comparison of Serum Stability against Uric Acid and Glucose. Research Journal of Pharmacy and Technology 2023; 16(4):1885-9. doi: 10.52711/0974-360X.2023.00309 Available on: https://rjptonline.org/AbstractView.aspx?PID=2023-16-4-58
REFERENCES:
1. Saha D, Jain VK, Jain B, Tandey R. Good laboratory practice: design and utility. Asian J Pharm Technol. 2011;1(1):1–3. Available at: https://ajptonline.com/AbstractView.aspx?PID=2011-1-1-1
2. Hartono H, Subarniati R, Pudjirahardjo WJ, Judajana FM. Manajemen Pengetahuan untuk Keselamatan Pasien. Indones J Clin Pathol Med Lab. 2016;20(3):253–9. http://dx.doi.org/10.24293/ijcpml.v20i3.474
3. Hedayati M, Razavi SA, Boroomand S, Kheradmand Kia S. The impact of pre-analytical variations on biochemical analytes stability: A systematic review. J Clin Lab Anal. 2020;34(12):1–15. https://doi.org/10.1002/jcla.23551
4. Kachhawa K, Kachhawa P, Varma M, Behera R, Agrawal D, Kumar S. Study of the Stability of Various Biochemical Analytes in Samples Stored at Different Predefined Storage Conditions at an Accredited Laboratory of India. J Lab Physicians. 2017;9(01):011–5. https://doi.org/10.4103/0974-2727.187928
5. Putri AE, Indrasari YN, Hernaningsih Y. The effect of storage and time of blood specimen examination of plasma prothrombin time and activated partial thromboplastin time stabilities. Indian J Forensic Med Toxicol. 2020;14(3):1673–8. Available at: https://repository.unair.ac.id/106358/
6. Muslim M, Suhariadi F, Damayanti NA, Purnomo W. The performance of medical laboratory technician based on situation awareness and psychological capital with the work engagement mediation. Indian J Public Heal Res Dev. 2018;9(1):199–202. Available at: https://repository.unair.ac.id/92678/
7. Rahmanitarini A, Hernaningsih Y, Indrasari YN. The stability of sample storage for complete blood count (CBC) toward the blood cell morphology. Bali Med J. 2019;8:482. https://doi.org/10.15562/bmj.v8i2.1369
8. Lestari A. Different Amount of Thrombocytes on Blood Storage for 24 Hours in Room and Refrigerator. J Vocat Heal Stud. 2019;3(2):59–62. https://doi.org/10.20473/jvhs.V3.I2.2019.59-62
9. Kahar H. Keuntungan dan kerugian penjaminan mutu berdasarkan uji memastikan kecermatan (POCT). Indones J Clin Pathol Med Lab. 2018;13(1):38–41. http://dx.doi.org/10.24293/ijcpml.v13i1.898
10. Cuhadar S, Koseoglu M, Atay A, Dirican A. The effect of storage time and freeze-thaw cycles on the stability of serum samples. Biochem Medica. 2013;23(1):70–7. https://doi.org/10.11613/bm.2013.009
11. Bowen RAR, Remaley AT. Interferences from blood collection tube components on clinical chemistry assays. Biochem Medica. 2014;24(1):31–44. https://doi.org/10.11613%2FBM.2014.006
12. Cuhadar S, Atay A, Koseoglu M, Dirican A, Hur A. Stability studies of common biochemical analytes in serum separator tubes with or without gel barrier subjected to various storage conditions. Biochem Medica. 2012;22(2):202–14. https://doi.org/10.11613/bm.2012.023
13. Gómez Rioja R, Martínez Espartosa D, Segovia M, Ibarz M, Llopis MA, Bauça JM, et al. Laboratory sample stability. Is it possible to define a consensus stability function? An example of five blood magnitudes. Clin Chem Lab Med. 2018;56(11):1806–18. https://doi.org/10.1515/cclm-2017-1189
14. Arslan FD, Karakoyun I, Basok BI, Aksit MZ, Baysoy A, Ozturk YK, et al. The local clinical validation of a new lithium heparin tube with a Barrier: BD vacutainer® barricor LH plasma tube. Biochem Medica. 2017;27(3):1–11. https://doi.org/10.11613%2FBM.2017.030706
15. Lima-Oliveira G, Lippi G, Salvagno GL, Montagnana M, Picheth G, Guidi GC. Preanalytical management: Serum vacuum tubes validation for routine clinical chemistry. Biochem Medica. 2012;22(2):180–6. https://doi.org/10.11613/bm.2012.021
16. Hegstad S, Spigset O, Helland A. Stability of 21 Antihypertensive Drugs in Serum Collected in Standard (Nongel) Serum Tubes Versus Tubes Containing a Gel Separator. Ther Drug Monit. 2020;42(2):335–40. https://doi.org/10.1097/ftd.0000000000000708
17. Setiawan B, Nugraheni UR, Rahayu M. Vacutainer serum separator sebagai alternatif tabung penampung darah pada pemeriksaan kadar ureum. J Muhammadiyah Med Lab Technol. 2021;4(1):81. http://dx.doi.org/10.30651/jmlt.v4i1.7447
18. Beeler MF. Medical Laboratory Technology. Am J Trop Med Hyg. 1963;12(5):835–835. https://doi.org/10.4269/ajtmh.1963.12.835
19. Guder WG. History of the preanalytical phase: A personal view. Biochem Medica. 2014;24(1):25–30. https://doi.org/10.11613%2FBM.2014.005
20. Bharath CL, Ramadurg B. Antidiabetic Activity of Phragmites karka (Retz.) in Alloxan Induced Diabetic Rats. Res J Pharmacol Pharmacodyn. 2013;5(6):371–5. Available at: https://rjppd.org/AbstractView.aspx?PID=2013-5-6-21
21. Abbas SH, Khdair SA. Study the Effect of Maternal Factors on Prevalence of Gestational Diabetes Mellitus and Effect of Treatment Types on Serum Blood Glucose. Res J Pharm Technol. 2021;14(10):5264–9. http://dx.doi.org/10.52711/0974-360X.2021.00917
22. Hassan N, Shahwan M, Alkhoujah S, Jairoun A. Association between serum liver enzymes and metabolic syndrome among type 2 diabetes mellitus patients. Age. 2021;56(9.8):59–83. https://doi.org/10.5958/0974-360X.2021.00188.8
23. Furqon A, Nurmukhlis H, Kasiman S. Stabilitas Konsentrasi Glukosa Darah Simpan Jangka Pendek Dalam Tabung Berteknologi Pemisah Jel. Pharmaciana. 2015;5(2):108–14. http://dx.doi.org/10.12928/pharmaciana.v5i2.2310
24. Tandi J, Danthy R, Kuncoro H. Effect of Ethanol Extract from Purple Eggplant Skin (Solanum melongena L) On Blood Glucose Levels and Pancreatic Β Cells Regeneration on White Rats Male Hypercholesterolemia-Diabetic. Res J Pharm Technol. 2019;12(6):2936–42. https://doi.org/10.5958/0974-360X.2019.00494.3
25. Sirajwala HB, Sharma D, Agravatt AM. A study of serum total calcium and uric acid levels in preeclampsia Abstract : Is now with. Indian J Basic Appl Med Res. 2013;(1):50–6. Available at: https://www.ijbamr.com/assets/images/issues/pdf/PDF%20DECEMBER%2013%20%2050-56.pdf.pdf
26. Faught RC, Marshall J, Bornhorst J. Solution densities and estimated total protein contents associated with inappropriate flotation of separator gel in different blood collection tubes. Arch Pathol Lab Med. 2011;135(9):1081–4. https://doi.org/10.5858/2010-0488-oar.1
27. Murtadha JH, Razzaq HA. Association of serum renal function levels with heart failure disease in iraqi patients. Res J Pharm Technol. 2021;14(5):2464–6. https://doi.org/10.5958/0974-360X.2019.00494.3
28. Dhivya S, Gangopadhyay S, Kumar P, Sarkar G. Serum creatinine and eGFR are affected in female hypothyroid patients with poor Thyroid control. Asian J Pharm Technol. 2020;10(4). http://dx.doi.org/10.5958/2231-5713.2020.00040.9
29. Mohammed OJ, Saeed AM, Mohammed IS. RP–HPLC Developed Method for Uric Acid Estimation in Human Serum. Res J Pharm Technol. 2019;12(10):4703–8. https://doi.org/10.5958/0974-360X.2019.00810.2
30. Nisa U, Astana PRW, Kuncoro H. The effect of Antihypertensive Herb formula of Indonesian traditional medicines against serum uric acid levels in mild Hypertensive patients. Res J Pharm Technol. 2021;14(1):254–8. http://dx.doi.org/10.5958/2231-5713.2020.00040.9
31. Kocijancic M, Cargonja J, Delic-Knezevic A. Evaluation of the BD Vacutainer® RST blood collection tube for routine chemistry analytes: Clinical significance of differences and stability study. Biochem Medica. 2014;24(3):368–75. https://doi.org/10.11613/bm.2014.039
32. Martsiningsi, Otnel. Gambaran Kadar Asam Urat Darah Metode Basah ( Uricase-PAP ) Pada Sampel Serum dan Plasma. J Teknol Lab. 2016;5(1):20–6. Available at: https://www.teknolabjournal.com/index.php/Jtl/article/view/72/51
33. Kösem A, Topçuoǧlu C, Sezer S, Turhan T, Cevher ŞK, Yenigün EC, et al. Comparison of some biochemical tests in different blood collection tubes in hemodialysis patients. Turkish J Biochem. 2020;45(1):26–36. http://dx.doi.org/10.1515/tjb-2018-0341
34. Erdal EP, Mitra D, Khangulov VS, Church S, Plokhoy E. The economic impact of poor sample quality in clinical chemistry laboratories: results from a global survey. Ann Clin Biochem. 2017;54(2):230–9. https://doi.org/10.1177/0004563216651647
35. Nwe WWP, Thwin AMM, San Yi M, Yee KT, Oo MS, Rahman NIBA, et al. Serum Uric Acid, Lipid Profile and Atherogenic Index of Plasma in Dipper and Non-dipper Normotensive Subjects. Res J Pharm Technol. 2021;14(5):2511–6. https://doi.org/10.52711/0974-360X.2021.00442