Author(s): Dina Louis, Amira Rashad


DOI: 10.52711/0974-360X.2023.00298   

Address: Dina Louis1,2*, Amira Rashad2
1Pharmaceutics and Industrial Pharmacy Dept., Faculty of Pharmacy, Cairo University, Kasr Eleini Street, 11562, Cairo, Egypt.
2Pharmaceutics and Pharmaceutical Technology Dept., Faculty of Pharmacy, Heliopolis University, 3 Cairo-Belbeis Road, Cairo, 11785, Egypt.
*Corresponding Author

Published In:   Volume - 16,      Issue - 4,     Year - 2023

Hair dyes have been used to cover gray hair or to give an appealing look. In the present study, the extract of Calendula Officinalis was used in preparing a hair dye. This represents a natural gateway in development of hair dye to avoid damage caused by synthetic dyes. The yellow coloring principle of the extract, Calendulin, was converted into nano-sized formula ensuring hair fiber penetration. Nine formulae were prepared by solvent evaporation technique, based on a 32 factorial design using Design Expert® program, independent variables being: speed and time of homogenization; three levels: low, medium and high. Polyvinyl alcohol (0.5% w/v) was used as stabilizing agent. Formulae were evaluated by determination of Zeta size, potential and polydispersity index. Response surface modeling enabled choosing the optimal formula (least particle size, polydispersity index and highest zeta potential), which was evaluated by transmission electron microscopy and dyeing effect as a gel containing 0.5% Carboxymethyl cellulose. F7, produced by operating the homogenizer at 26000rpm for 10 minutes proved to be optimal which succeeded to dye white hair and lighten up the black hair as evidenced by optical microscope images. Hence it was recommended to use F7 in covering the white hair.

Cite this article:
Dina Louis, Amira Rashad. Formulation of Nano-sized dispersion from Calendula Officinalis: A Natural gateway to preparation of a Hair Dye. Research Journal of Pharmacy and Technology 2023; 16(4):1815-2. doi: 10.52711/0974-360X.2023.00298

Dina Louis, Amira Rashad. Formulation of Nano-sized dispersion from Calendula Officinalis: A Natural gateway to preparation of a Hair Dye. Research Journal of Pharmacy and Technology 2023; 16(4):1815-2. doi: 10.52711/0974-360X.2023.00298   Available on:

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