Author(s):
M. V. Kumudhavalli, S. Sangeetha, S. Alexander, R. Shivananthan, B. S. Venkateswarlu, Kumar. M
Email(s):
kumudhu27@gmail.com
DOI:
10.52711/0974-360X.2023.00264 
Address:
M. V. Kumudhavalli*, S. Sangeetha, S. Alexander , R. Shivananthan, B. S. Venkateswarlu, Kumar. M
Department of Pharmaceutical Analysis, Vinayaka Missions Research Foundation (Deemed University), Yercaud Main Road, Kondappanaikenpatty, Vinayaka Mission’s Research l Salem, Tamilnadu 636008.
*Corresponding Author
Published In:
Volume - 16,
Issue - 4,
Year - 2023
ABSTRACT:
Suppositories very popular formulations and are currently used therapeutically all over the world. Especially in pediatrics, where they can be used for the effective lowering of high fever accomplished with seizure. The choice of a suppository as the mode of drug delivery system in all cases even in infants when oral delivery is impossible, that is an unconscious or vomiting patient, or in the case of infants. Therefore, the quality control study of this dosage form with a modern instrumental analytical technique is highly desired. In consequence of the increased application of suppositories, there is a current demand for a rapid, effective analytical methods required for routine analysis. The literature search revealed that methods for the UV and high-performance liquid chromatography (HPLC) analysis of DZP are very rare.Thereforethe aim of the present research was to develop and validate a suitable general sample preparation and chromatography method for suppositories containing DZP. The primary method (I) is based on UV Spectroscopy. UV spectra of Diazepam are recorded. The secondary method is based on the Reverse phase HPLC method was developed. The UV detector was operated at 286nm. After method development the proposed method was validated as per ICH guidelines. The developed method was accurate and precise which shows the evident from the analytical data and recovery studies. The analytical results were in good and it complies with the label claims.
Cite this article:
M. V. Kumudhavalli, S. Sangeetha, S. Alexander , R. Shivananthan, B. S. Venkateswarlu, Kumar. M. Extraction and Quantification of Ano Rectal Formulation by UV-LC Techniques. Research Journal of Pharmacy and Technology 2023; 16(4):1617-1. doi: 10.52711/0974-360X.2023.00264
Cite(Electronic):
M. V. Kumudhavalli, S. Sangeetha, S. Alexander , R. Shivananthan, B. S. Venkateswarlu, Kumar. M. Extraction and Quantification of Ano Rectal Formulation by UV-LC Techniques. Research Journal of Pharmacy and Technology 2023; 16(4):1617-1. doi: 10.52711/0974-360X.2023.00264 Available on: https://rjptonline.org/AbstractView.aspx?PID=2023-16-4-12
REFERENCES:
1. El-Hawary WF, Issa YM, Talat A. Spectrophotometric Determination of Diazepam in Pure form, Tablets and Ampoules. Int J Biomed Sci. 2007 Mar;3(1): 50-5. PMCID: PMC3614613 PMID: 23675021
2. Dastidar DG, Sa B. A comparative study of UV-spectrophotometry and first-order derivative UV-spectrophotometry methods for the estimation of diazepam in presence of Tween-20 and propylene glycol. AAPS PharmSciTech. 2009;10(4):1396-400.doi: 10.1208/s12249-009-9343-x
3. Kaura, A., Gupta, V., Roy, G. S., and Kaura, M. Spectrophotometric determination of chlorpheniramine maleate and phenylpropanolamine hydrochloride in dosage forms. International Current Pharmaceutical Journal, (2013). 2(5), 97–100. https://doi.org/10.3329/icpj.v2i5.14436
4. Ali Mohammad Akhoundi-Khalafi and Masoud Reza Shishehbore, A New Technique for Quantitative Determination of Dexamethasone in Pharmaceutical and Biological Samples Using Kinetic Spectrophotometric Method, International Journal of Analytical Chemistry, vol.2015, Article ID439271, 6 pages, 2015. https://doi.org/10.1155/2015/439271
5. Basavaiah K, Krishnamurthy G. Extractive spectrophotometric determination of some phenothiazine derivatives in pharmaceutical preparations. Talanta. 1998 Aug;46(4):665-70. https://doi.org/10.1016/S0039-9140(97)00323-8
6. Salem AA, Barsoum BN, Izake EL. Spectrophotometric and fluorimetric determination of diazepam, bromazepam and clonazepam in pharmaceutical and urine samples. Spectrochim Acta A Mol Biomol Spectrosc. 2004 Mar;60(4):771-80.https://doi.org/10.1016/S1386-1425(03)00300-7
7. Mannucci C, Bertini J, Cocchini A, Perico A, Salvagnini F, Triolo A. Simultaneous determination of otilonium bromide and diazepam by first-derivative spectroscopy. J Pharm Sci. 1992 Dec;81(12):1175-7. https://doi.org/10.1002/jps.2600811209
8. Furlanetto S, Orlandini S, Massolini G, Faucci MT, La Porta E, Pinzauti S. Optimisation and validation of a capillary electrophoresis method for the simultaneous determination of diazepam and otilonium bromide. Analyst. 2001 Oct;126(10):1700-6. https://doi.org/10.1039/B103091B
9. Sreejith K.R.*, Rajagopal P.L., Premaletha K. Analytical Method Development and Validation of Anostrozole in Pure and Tablet Dosage Form by UV Spectroscopy. Research J. Pharm. and Tech. 2017; 10(4): 1015-1019.https://doi.org/10.5958/0974-360X.2017.00183.4
10. Monika Maheshwari, Parul Soni. RP-HPLC Method development and Validation for Rapid estimation of Diazepam in Bulk and Pharmaceutical Dosage Form. Research Journal of Pharmacy and Technology. 2022; 15(5):1938-2. https://doi.org/10.52711/0974-360X.2022.00322
11. Tanaka M, Wang Q, Morikawa Y, Tsukamoto J, et al Efficacy, safety, and economic impact of diazepam suppositories with as-needed acetaminophen for prevention of seizure recurrence during the same fever episode in children with suspected simple febrile seizures. Epilepsia. 2022 Jul; 63(7): 1704-1713. https://doi.org/10.1111/epi.17271