Author(s): Shubhangi A. Patil, Pratibha S. Salve, Rohan S. Phatak, Niranjan D. Chivate

Email(s): , , ,

DOI: 10.52711/0974-360X.2023.00172   

Address: Shubhangi A. Patil1*, Pratibha S. Salve2, Rohan S. Phatak3, Niranjan D. Chivate4
1Research Scholar KIMS Deemed to be University, Karad 415539, Maharashtra, India.
2Asst. Professor, Department of Pharmacology, KIMS Deemed to be University, Karad 415539. Maharashtra, India.
3Jr. Research Officer, Directorate of Reserch, KIMS Deemed to be University, Karad 415539. Maharashtra, India.
4Sr. Lecturer KCT’S Krishna College of Pharmacy, Karad, 415539 Maharashtra, India.
*Corresponding Author

Published In:   Volume - 16,      Issue - 3,     Year - 2023

In the present study, Total phenolic content, total flavonoid content and in-vitro antioxidant activity of Psidium guajava L. (Myrtecae) leaves extracts (aqueous and methanolic) were estimated quantitatively by using spectrophotometric methods. Antioxidant activity of extracts were expressed as percentage of DPPH radicals inhibition.AEPG shows 40% whereas, MEPG shows 56.66% inhibition.The total phenolic contents (TPC) were calculated by using standard curve of gallic acid and TPCof AEPG and MEPG were 37.72 mg GAE/g and 84.91 mgGAE/g of dry weight of extract respectively .Whereas, total flavonoid contents (TFC) were calculated by using standard calibration curve of Quercetin and TFC of AEPG and MEPG were 67.37 mg QE/g and 86.75 mg QE/g of dry weight of extract correspondingly. MEPG showed the highest phenolic and flavonoid contents. The high content of phenolic compounds indicated that Psidium guajava having potent antioxidant activity and can become one of the promising agent from natural plant source of antioxidant and widely used as folk medicine.

Cite this article:
Shubhangi A. Patil, Pratibha S. Salve, Rohan S. Phatak, Niranjan D. Chivate. Quantitative Estimation of Total Phenolic, Total Flavonoid content and Assessment of In-Vitro Antioxidant Capacity of Psidium guajava L. Leaves Extracts. Research Journal of Pharmacy and Technology 2023; 16(3):1028-2. doi: 10.52711/0974-360X.2023.00172

Shubhangi A. Patil, Pratibha S. Salve, Rohan S. Phatak, Niranjan D. Chivate. Quantitative Estimation of Total Phenolic, Total Flavonoid content and Assessment of In-Vitro Antioxidant Capacity of Psidium guajava L. Leaves Extracts. Research Journal of Pharmacy and Technology 2023; 16(3):1028-2. doi: 10.52711/0974-360X.2023.00172   Available on:

1.    Song FL et al. Total phenolic contents and antioxidant capacities of selected chinese medicinal Plants. International Journal of Molecular Sciences.2010;6(11):2362-72. doi: 10.3390/ijms11062362.
2.    Joshi UP. Wagh RD.Estimation of total phenolic, total flavonoid content and assessment of in vitro antioxidant activity of extracts of plant Ehretialaevis bark. Journal Of Pharmacy And Biological Sciences. 2018;13(6) :13-21. doi: 10.9790/3008-1306021322.
3.    Salah EM et al. In vitro Antioxidant Activity, Total Phenolics and Flavonoids Contents of Gladiolus segetum Extracts. Research J. Pharm. and Tech 2018; 11(11): 5017-5023. doi: 10.5958/0974-360X.2018.00915.0
4.    Som K et al. Phytochemical analysis, Total Flavonoid, Phenolic contents and Antioxidant activity of extracts from the Leaves of Rhododendron arboreum. Research J. Pharm. and Tech. 2020; 13(4):1701-1706. doi: 10.5958/0974-360X.2020.00307.8
5.    Kavitha S et al. Phytochemical Studies and Quantification of total content of Phenols, Tannins and Flavonoids in selected endangered plant species. Res. J. Pharmacognosy and Phytochem. 2018; 10(4): 277-281. doi: 10.5958/0975-4385.2018.00044.4
6.    Panchaksharam S, Kuravappullam VR. Estimation of phenolic compounds present in the plant extracts using high pressure liquid chromatography, antioxidant properties and its antibacterial activity. Indian Journal of Pharmaceutical Education and Research. 2018; 52(2) : 321-326. doi: 10.5530/ijper.52.2.37
7.    Kim JH et al. Turmeric (Curcuma longa) inhibits inflammatory nuclear factor (NF)-kB and NF-kB-regulated gene products and induces death receptors leading to suppressed proliferation,induced chemo sensitization, and suppressed osteoclastogenesis. Molecular Nutrition and Food Research. 2012; 56 (3) : 454–465. doi: 10.1002/mnfr.201100270.
8.    Jagadish LK et al.Comparative study on the antioxidant, anticancer and antimicrobial property of agaricus bisporusimbach before and after boiling. African Journal of Biotechnology. 2009; 8(4) : 654–661.doi:
9.    Pelegrini PB, et al.Identification of a novel storage glycine-rich peptide from guava (Psidium guajava) seeds with activity against Gram-negative bacteria, Peptides.2008;29(8):1271-1279.doi: 10.1016/j.peptides.2008.03.013.
10.    Bontempo P et al. Psidium guajava L. anti-neoplastic effects: induction of apoptosis and cell differentiation. Cell Prolif .  2012;45(1):22-31.doi: 10.1111/j.1365-2184.2011.00797.x.
11.    Banu MS, Sujatha K. Antimicrobial potential of ethanolic extract of Psidium guajava leaf and its isolated fraction against some pathogenic microorganisms. Research J. Pharmacology and Pharmacodynamics. 2012;4(3): 169-171.
12.    Shetty P et al. Wound Healing Potential of Psidium guajava var. Pyrifera. Research J. Pharm. and Tech. 2019; 12(12): 6067-6070. doi: 10.5958/0974-360X.2019.01053.9
13.    Barbalho SM et al. Psidium guajava (Guava): A plant of multipurpose medicinal applications. Medicinal & Aromatic Plants.2012;1(4):1-6. doi : 10.4172/2167-0412.1000104.
14.    Joseph B.Priya M. Review on nutritional, medicinal and pharmacological properties of guava (Psidium guajava Linn.). International Journal of Pharma And Bio Sciences.2011; 2 (1) : 53-69.
15.    Simão AA extract of Psidium guajava leaves: Phenolic compounds and inhibitory potential on digestive enzymes.Annals of the Brazilian Academy of Sciences.2017; 89 (3 Suppl.): 2155-2165.
16.    Zhang B. at,el. Phenolic profiles of 20 canadian lentil cultivars and their contribution to antioxidant activity and inhibitory effects on a-Glucosidase and pancreatic lipase. Food Chemistry. 2015; 1(172) : 862-872. doi: 10.1016/j.foodchem.2014.09.144.
17.    Vogel P et al. Polyphenols benefits of Olive leaf (Olea Europaea L.) to human health. Nutricon Hospitalaria. 2015; 31(3): doi:1427-1433. :10.3305/nh.2015.31.3.8400
18.    Jayakumari S. et al. Evaluation of toothache Activity of methanolic extract and its various fractions from the leaves Psidium guajava Linn. International Journal of Pharma and Bio Sciences. 2012 ; 3 (2): 238-249.
19.    Wadkar KA, Magdum CS. Evaluation of Total Phenolic Content, Flavonoid Content and Antioxidant Activity of Stem Bark of Careya arborea Roxb. Research J. Pharmacognosy and Phytochemistry. 2010; 2(1): 49-51.
20.    Shinde S et al. Hypolipidemic activity of Psidium  guajava linn leaves extracts in hyperlipidemic  rats. International Journal of Pharmacy and Pharmaceutical Sciences. 2013; 5(1):70-72.doi:
21.    Kokate CK, Purohit AP, Gokhale SB, Pharmacognosy, Practical Pharmacognosy.16th ed. Pune: Nirali Publishers, 1993, 493-497.
22.    Rao N, Mittal S, Sudhanshu, Menghani E. Assessment of Phytochemical Screening, Antioxidant and Antibacterial Potential of the Methanolic Extract of Ricinus communis L. Asian J. Pharm. Tech. 3(1):  2013; 20-25.
23.    Muchandi AA. Dhawale SC.  Estimation of total phenolic contents, total flavonoid contents and     muscle  co-ordination activity of ethanolic extract of Stereospermum suaveolens DC. International Journal of Research in Pharmaceutical and Nano Sciences,2017; 6(3): 118 - 124.
24.    Kamtekar S et al. Estimation of phenolic content, flavonoid content, antioxidant and alpha amylase    inhibitory activity of marketed polyherbal formulation.Journal of Applied Pharmaceutical Science. 2014;4(9): 061-065.doi : 10.7324/JAPS.2014.40911.
25.    Elayarani M, Shanmuganathan P, Muthukumaran P. In Vitro Anti-Oxidant Activity of the Various Extracts of Cassia auriculata L. Flower by UV Spectrophotometer. Asian J. Pharm. Tech. 2011; 1(3): 70-72.
26.    Phatak RS et al. Investigation of antioxidant and antidiabetic activities of Murrayya koenigii leavesmethanolic and aqueous extract by methods.International Journal of Research in Pharmaceutical Sciences. 2018; 9(4):1460-1464.doi 10:7324/JAPS.2014.40911.
27.    Tamboli FA , More HN, Bhandugare SS , Patil1AS , Nilawar ND , Jadhav NR EJPMR, 2020; 7(8) : 571-574.
28.    Amini MH. et al. Phytochemical Screening and Antioxidant Activity of Heracleum afghanicum Kitamura leaves. Research Journal of Pharmacy and Technology. 2017; 10(10):3498-3502. doi: 10.5958/0974-360X.2017.00626.6.
29.    Beena WS et al. Antioxidative Activity of Psidium guajava Leaf Extract- A Review. Research J. Pharmacognosy and Phytochemistry 2011; 3(4): 143-147.
30.    Melakhessou MA, Benkiki N, Marref SE. Determination of  Antioxidant  Capacity, Flavonoids and Total Phenolic Content of Extracts from Atractylis flava Desf. Research J.  Pharm. and Tech 2018; 11(12): 5221-5226. doi: 10.5958/0974-360X.2018.00952.6
31.    Rajendran R, Basha NS, Ruby S. Evaluation of In Vitro Antioxidant Activity of stem-bark and stem-wood of Premna serratifolia Lin., (Verbenaceae). Research J. Pharmacognosy and Phytochemistry. 2009; 1(1):11-14.

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