Bandana Sharma, Chidambaram Soundrapandian, Sonam Bhutia
Bandana Sharma1*, Chidambaram Soundrapandian2, Sonam Bhutia3
1,3Government Pharmacy College Sajong, Government of Sikkim, Sikkim University, Sajong, Rumtek, East Sikkim, India – 737135.
2College of Health Science, Debre Tabor University, P.O. Box 272, Debre Tabor, Ethiopia.
Volume - 16,
Issue - 3,
Year - 2023
The present study aim to prepare and characterize prolonged local drug delivery of antimicrobial on bones tissue formation and its In-vitro evaluation. The results of drug released pattern were analysed after coating. In formulation (F1), the drug release was steep till 73%, where coating showed no such retardation effect. After 73% of drug release, F1 showed prolonged release of drug even after coating with 1% and 2% Chitosan, F2 and F3 showed prolonged better effect with 1% as compared to 2% Chitosan coating. In formulations containing one part of bioglass (F4 and F6) Showed prolonged release by 2% coating followed by 1% Chitosan coating. F5 was the exception where coating favoured dissolution. F8 and F9 showed prolonged release of drug by 1% than 2% Chitosan. In-case of In-vitro study, concentration was maintained above MIC i,e above 0.032 except in 816 hours where the drug concentration was observed below MIC (Fig No-14). F4C was found to be the best i,e 0.880 (Table No-06) for prolonged drug release over 6 weeks. FTIR study confirmed that characteristic peaks showed by Moxifloxicin (Fig No-3 & Table No-5) were 719cm-1, 1045cm-1, 1702cm-1 and 3327cm-1 which were also shown by developed formulations (725cm-1, 1051cm-1, 1703cm-1 and 3543cm-1). For In-vitro bioactivity, SEM were also performed after 24hrs (Fig No-15) and 48hrs (Fig No-16), the intensity of the HAP in 48hrs was more as compared to 24rs which confirmed that formulation is able to induced new bone cell after implantation. This result indicates that these newly prepared formulations could be a potential drug delivery system in osteomylietis condition (induces new bone cell) and can be helpful for the scientists for the particular field of study in near future.
Cite this article:
Bandana Sharma, Chidambaram Soundrapandian, Sonam Bhutia. Formulation, Characterization and In-vitro Evaluation of Prolonged Local Drug Delivery of Antimicrobial on Bone Tissue Formation. Research Journal of Pharmacy and Technology 2023; 16(3):1147-2. doi: 10.52711/0974-360X.2023.00191
Bandana Sharma, Chidambaram Soundrapandian, Sonam Bhutia. Formulation, Characterization and In-vitro Evaluation of Prolonged Local Drug Delivery of Antimicrobial on Bone Tissue Formation. Research Journal of Pharmacy and Technology 2023; 16(3):1147-2. doi: 10.52711/0974-360X.2023.00191 Available on: https://rjptonline.org/AbstractView.aspx?PID=2023-16-3-27
1. Concia E, Prandini N, Massari L, Ghisellini F, Consoli V, Menichetti F. Osteomyelitis: clinical update for practical guidelines. Nuclear Medicine Communication.2006:27(8):645-660.doi:10.1097/00006231-200608000-00007.
2. Lalitha, P., Sachithanandam, V., Swarnakumar, N. S., & Sridhar, R. Review on Anti-inflammatory Properties of Mangrove plants. Asian Journal of Pharmaceutical Research. 2019: 9(4), 273-288.doi: 10.5958/2231-5691.2019.00045.5.
3. Walter G, Kemmerer M, Kappler C, Hoffmann R: Treatment algorithms for chronic osteomyelitis. Duetsches Arzteblatt International. 2012: 109(14): 257–264.doi: 10.3238/arztebl.2012.025.
4. Gardner MJ, Demetrakopoulos D, Shindle MK, Griffith MH, Lane JM. Osteoporosis and skeletal fractures. Health and Services Journal. 2006: 2 (1):62–69.doi: 10.1007/s11420-005-0137-8.
5. Ginebra MP, Traykova T and Planell JA. Calcium phosphate cements: competitive drug carriers for the musculoskeletal system. Biomaterials. 2006: 27(10):2171–2177.doi: 10.1016/j.biomaterials.2005.11.023.
6. Calhoun, J. H., & Manring, M. M. (2005). Adult osteomyelitis. Infectious Disease Clinics, 19(4), 765-786. doi: 10.1016/j.idc.2005.07.009.
7. Ikpeme, I. A., Ngim, N. E., & Ikpeme, A. A. Diagnosis and treatment of pyogenic bone infections. African health sciences. 2010: 10(1), 82. PMID: 20811530; PMCID: PMC2895795.
8. El-Husseini M, Patel S, MacFarlane RJ and Haddad FS. Biodegradable antibiotic delivery systems. Journal Bone Joint Surgery. 2011: 93(2): 151-157.doi: 10.1302/0301-620X.93B2.24933.
9. Salve, P. S. Development and in vitro evaluation colon targeted drug delivery system using natural gums. Magnesium. 2011: 61(41): 21.doi: 10.1080/1061186021000072690.
10. Patel, N., Nagesh, C., Chandrashekhar, S., Jinal, P., & Devdatt, J. Floating drug delivery system: An innovative acceptable approach in gastroretentive drug delivery. Research journal of pharmaceutical dosage forms and technology. 2012: 4(2), 93-103.doi: 10.5958/2231-5691.2020.00009.X.
11. Makeshwar, K. B., & Wasankar, S. R. Niosome: a novel drug delivery system. Asian journal of pharmaceutical research. 2013: (1): 16-20.doi.org/10.1248/bpb.34.945.
12. Mehta S., Humphrey JS, Dchenkmann DI, Seaber AV. Gentamicin distribution from collagen carrier. Journal Orthopaedic Research. 1996: 14(5): 749-754.https://doi.org/10.1002/jor.1100140511.
13. Bhowmik, D., Bhanot, R., Gautam, D., Rai, P., & Kumar, K. S. Osteomyelitis-symptoms, causes and treatment. Research Journal of Science and Technology. 2018: 10(2): 165-177.doi:10.5958/2349-2988.2018.00024.4.
14. Buchholz HW, Elson RA, Engelbrecht E, Lodenkamper H, Rottger J and Siegel A. Management of deep infection of total hip replacement. The Journal of Bone & Joint Surgery.1981:63:342–353. https://doi.org/10.1302/0301-620X.63B3.7021561.
15. S Bharati, C Soundrapandian, D Basu, S Datta. Studies on a novel bioactive glass and composite coating with hydroxyapatite on titanium based alloys: Effect of γ sterilization on coating. Journal of the European Ceramic Society. 2009: 29(12): 2527–2535. https://doi.org/10.1016/j.jeurceramsoc.2009.02.013.
16. Nep, E. I., & Conway, B. R. Preformulation studies on grewia gum as a formulation excipient. Journal of thermal analysis and calorimetry. 2012: 108(1): 197-205. https://doi.org/10.1007/s10973-011-1782-4.
17. Housheh, S., Trefi, S., & Chehna, M. F. Identification and Characterization of Prasugrel Degradation Products by GC/MS, FTIR and 1H NMR. Asian Journal of Pharmaceutical Analysis. 2017: 7(2):55-66.doi: 10.5958/2231-5675.2017.00010.2.
18. Padmavathi, Y., Anjali, A., Babu, N. R., & Kumar, P. R. Development and validation of new FTIR method for quantitative analysis of gliclazide in bulk and pharmaceutical dosage forms. Asian Journal of Research in Chemistry. 2017: 10(3): 377-382.doi:10.5958/0974-4150.2017.00064.5.
19. Harding, L., Qi, S., Hill, G., Reading, M., & Craig, D. Q. M. The development of microthermal analysis and photothermal microspectroscopy as novel approaches to drug–excipient compatibility studies. International journal of pharmaceutics. 2008: 354(1-2): 149-157. https://doi.org/10.1016/j.ijpharm.2007.11.009.
20. C Soundrapandian, S Datta,B Kundu,D Basu, and B Sa. Porous Bioactive Glass Scaffolds for Local Drug Delivery in Osteomyelitis:Development and In Vitro Characterization. AAPS Pharm Sci Tech 2010: 11(4):1675-1683. https://doi.org/10.1208/s12249-010-9550-5.
21. Merchant HA, Shoaib HM, Tazeen J. Once-daily tablet formulation and in-vitro release evaluation of cefpodoxime using hydroxypropyl methylcellulose: A technical note. AAPS Pharm Sci Tech 2006: 7: 78-80.
22. Bourne DW, Pharmacokinetics, In Modern Pharmaceutics, New York.2002:4:67-92.https://doi.org/10.1208/pt070378.
23. Korsmeyer R W, Gurny R, Docler E, Peppas N E. Mechanism of solute release from porous hydrophilic polymers. International Journal of Pharmaceutics. 1983: 15: 25-35.https://doi.org/10.1016/0378-5173(83)90064-9.
24. Peppas N.A. Analysis of Fickian and non-Fickian drug release from polymers. Pharmaceutica Acta Helvetiae. 1985: 60:110-111.PMID: 4011621.
25. Labbaf, S. Spherical Sub-micron Bioactive Glass Particles and Their Interaction with Cells In Vitro. 2011.
26. Hatcher, B. M. Bioactive Organic-inorganic Hybrids for Tissue Engineering Applications (Doctoral dissertation, University of Florida) 2004.https://doi.org/10.25560/9136.
27. Pecheva, E. Study of Biocompatible and Biological Materials: Can they be influenced by external factors? Materials Research Forum LLC. 2017.
28. RAI, B. The evaluation of bioactive polycaprolactone scaffolds as protein delivery systems for bone engineering applications. 2006.
29. M.C. Purohit, Anuj Kandwal, Reena Purohit, A.R. Semwal, Shama Parveen, Arun K. Khajuria. Antimicrobial Activity of Synthesized Zinc Oxide Nanoparticles using Ajuga bracteosa Leaf Extract. Asian Journal of Pharmaceutical Analysis. 2021; 11(4):275-0.doi: 10.52711/2231-5675.2021.00047.
30. Kumar, K., & Singh, B. K. synthesis, characterization and anti-microbial activity of some 4-thiazolidinone conjugatives. Asian Journal of Pharmaceutical Analysis. 2020: 10(4).doi: 10.5958/2231-5675.2020.00036.8.