Basima J. Mohammad, Jabbar A. A. Al-Saaidi, Dirgham H. Y. AL_Zwean
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Basima J. Mohammad1, Jabbar A. A. Al-Saaidi2, Dirgham H. Y. AL_Zwean3
1Dept. Public Health, College of Veterinary Medicine, University of Al- Qadisiyah, Al-Qadisiyah, Iraq.
2Dept. Physiology, College of Veterinary Medicine, University of Al- Qadisiyah, Al-Qadisiyah, Iraq.
3Dept. Public Health, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq.
Volume - 16,
Issue - 3,
Year - 2023
The goal of this study was to document the toxic effects of aflatoxin B1 (AfB1) on the testis, epididymis, liver, and kidneys of adult male rats, and to employ camel milk as a natural antidote to neutralize these effects. For these purposes, 120 adult male Wister rats (90 days old) were divided into four groups (30 males each); control (C) group (drinking water was supplied), Cm group (camel milk was supplied at 10ml/kg bw/day), Af group (AfB1 was supplied at 0.3mg/kg bw/day), and CmAf group (combination treatment were supplied). The males were weighed and sacrificed on days 21 and 42 of the treatment to evaluate the histological changes of the liver, kidneys, testes, and epididymis. The body weight and testicular, epididymal, prostate, and seminal vesicle weights in the Af group decreased significantly, however this was improved in AfCm group. At day 21, histological findings of AF group revealed a decline of testicular germ layers and spermatogenesis arrest, necrotic and degenerative changes of hepatocytes and renal tubules, epididymal epithelial hyperplasia with cytoplasmic vacuolation, and depletion of sperms from the epididymis' lumen. At day 42, the severity of the histopathological changes were time-dependet. At both experimental periods, AfCm group reported substantial reduction in the degree of germ epithelium with normal seminiferous tubules epithelia with active spermatogenesis, necrosis of some hepatocytes beside infiltration of some inflammatory cells, most glomeruli and tubules were normal but some with sloughing tubular epithelia, and normal epididymalpseudostratified columnar epithelium with sperms in the lumen.
Cite this article:
Basima J. Mohammad, Jabbar A. A. Al-Saaidi, Dirgham H. Y. AL_Zwean. The Protective Role of Camel Milk against Reprotoxicity, Hepatotoxicity, and Nephrotoxicity in Aflatoxic-Induced Male Rats. Research Journal of Pharmacy and Technology 2023; 16(3):1072-8. doi: 10.52711/0974-360X.2023.00179
Basima J. Mohammad, Jabbar A. A. Al-Saaidi, Dirgham H. Y. AL_Zwean. The Protective Role of Camel Milk against Reprotoxicity, Hepatotoxicity, and Nephrotoxicity in Aflatoxic-Induced Male Rats. Research Journal of Pharmacy and Technology 2023; 16(3):1072-8. doi: 10.52711/0974-360X.2023.00179 Available on: https://rjptonline.org/AbstractView.aspx?PID=2023-16-3-15
1. Galali Y. Al-Dmoor HM. Miraculous Properties of Camel Milk and Perspective of Modern Science. J Fam Med Dis Prev. 2019;5(1):1–7. DOI: 10.23937/2469-5793/1510095
2. Ali A. Baby B.Vijayan R. From desert to medicine: A review of camel genomics and therapeutic products. Front Genet. 2019; 10(2):1–20. DOI: 10.3389/fgene.2019.00017.
3. Mirmiran P.Ejtahed HS.Angoorani P.Eslami F.Azizi F. Camel milk has beneficial effects on diabetes mellitus: A systematic review. Int J EndocrinolMetab. 2017;15(2):42150. DOI: 10.5812/ijem.42150.
4. Aitken RJ.Koopman P. Lewis SEM. Seeds of concern. Nature 2004;432(7013):48–52. DOI: 10.1038/432048a.
5. Swan SH. Elkin EP.Fenster L. The question of declining sperm density revisited: An analysis of 101 studies published 1934-1996. Environ Health Perspect 2000; 108(10):961–6. http://ehpnet1.niehs.nih.gov/docs/2000/108p961-966swan/abstract.html.
6. Shenuka S.Thiyagarajan T.Sambath Kumar R. A Review on the Effect of Immunosuppressants on Fertility. Research J. Pharm. and Tech. 2019; 12(3): 1441-1447.DOI: 10.5958/0974-360X.2019.00239.7.
7. Herath CB. Jin W. Watanabe G.et al. Adverse effects of environmental toxicants, octylphenol and bisphenol A, on male reproductive functions in pubertal rats. Endocrine 2004; 25(2):163–72. DOI:10.1385/ENDO:25:2:163.
8. Sharpe RM. Lifestyle and environmental contribution to male infertility. Br Med Bull. 2000;56(3):630–42. DOI: 10.1258/0007142001903436.
9. Alnaemi HS. Estimation of Aflatoxin M1 Levels in Some Dairy Products Manufactured from Raw Milk Experimentally Inoculated with Toxin. Iraqi J Vet Med. 2019;43(1):50–8. DOI:10.30539/iraqijvm.v43i1.471.
10. Marijani E.Charo-Karisa H.Gnonlonfin GJB. Et al. Effects of aflatoxin B1 on reproductive performance of farmed Nile tilapia. Int J Vet Sci Med. 2019;7(1):35–42. DOI: 10.1080/23144599.2019.1678315.
11. Parveen A. Singh A.Rajendiran A. et al. Herbal elicited Hepatoprotection and Hepatotoxicity – A Comprehensive Review. Asian J. Pharm. Res. 2022; 12(2):155-1.DOI: 10.52711/2231-5691.2022.00024.
12. Rathore P.Rao SP. Roy A. et al. Hepatoprotective Activity of Isolated Herbal Compounds. Research J. Pharm. and Tech. 2014; 7(2): 229-234.
13. Jwad SM. Abbas B.Jaffat HS. Study of The Protective Effect of Vitamin C plus E on Lincomycin-Induced Hepatotoxicity and Nephrotoxicity. Research J. Pharm. and Tech. 2015, 8(2): 177-184.DOI:10.5958/0974-360X.2015.00032.3.
14. Abdullah AG.Sedeeq BI.Azzubaidi MS. Histopathological changes in liver tissue after repeated administrations of an intermediate dose of coenzyme Q10 to Wistar Rats. Research J. Pharm. Tech. 2021; 14(8): 4025-8.DOI:10.52711/0974-360X.2021.00697.
15. Rani VU.Sudhakar M. Ramesh A. Protective effect of Puerariatuberosa Linn. in arsenic induced nephrotoxicity in rats. Asian J. Pharm. Res. 2017; 7(1): 15-20.DOI: 10.5958/2231-5691.2017.00003.X.
16. Mansoori AN.Gautam RK. and Tiwari PC. A Review on Genotoxicity. Asian J. Pharm. Res. 2014; 4(3): 162-165.https://asianjpr.com/AbstractView.aspx?PID=2014-4-3-7.
17. Banu N.Muthumary JP. Screening of aflatoxigenic property of some Aspergillusflavus isolated from sunflower seeds and its products at sunflower oil refineries. Research J. Science and Tech. 2010; 2(5): 102-107.https://rjstonline.com/AbstractView.aspx?PID=2010-2-5-4.
18. Khalil R.Yassin M.Yaseen SJ. Detection some Aflatoxins in some locally marketed Raw Peanuts. Research J. Pharm. and Tech 2021; 14(3):1431-1437.DOI: 10.5958/0974-360X.2021.00255.9.
19. Sukmanadi M. Effendi MH. The protective effect of Capsaicin (Capsicum annum L) against the induction of Aflatoxin B1 in hepatocytes: A study of liver Histopathology in mice (Musmusculus). Research J. Pharm. and Tech. 2021; 14(2):813-816.DOI: 10.5958/0974-360X.2021.00143.8.
20. Albonico M. Schutz LF. Caloni F. et al, In vitro effects of the Fusarium mycotoxins fumonisin B1 and beauvericin on bovine granulosa cell proliferation and steroid production. Toxicon, 2017; 128(3), 38–45. DOI:10.1016/j.toxicon.2017.01.019.
21. Park H. Park HS. Lim W. Song G. Ochratoxin A suppresses proliferation of Sertoli and Leydig cells in mice. Medical Mycology, 2020; 58(1), 71–82. DOI:10.1093/mmy/myz016.
22. Khoury Del. Fayjaloun S. Nassar M. et al. Updates on the effect of mycotoxins on male reproductive efficiency in mammals. Toxins, 2019; 11(9), 515–536. DOI:10.3390/toxins11090515.
23. Adedara IA. Nanjappa MK. Farombi EO. Et al. Aflatoxin B1 disrupts the androgen biosynthetic pathway in rat Leydig cells. Food and Chemical Toxicology : An International Journal Published for the British Industrial Biological Research Association, 2014; 65(3), 252–259. DOI:10.1016/J.FCT.2013.12.027.
24. Kudayer AM.Alsandaqchi ATA.Saleh FM.Alwan NA. Toxic Effect of Aflatoxin B1 on Heart, Lung, and Testis of Male Albino Rats: Histopathology Study. IOP Conf. Ser.: Mater. Sci. Eng., 2019; 571 012055. DOI:10.1088/1757-899X/571/1/012055.
25. Bilinska B. Hejmej A. Kotula-Balak M. Preparation of Testicular Samples for Histology and Immunohistochemistry. Methods in Molecular Biology (Clifton, N.J.). 2018. 1748, 17–36. DOI:10.1007/978-1-4939-7698-0_3.
26. Schefler WC. Statistics for the biological sciences. Addison- Wesley Publishing Company, London, 2nd ed., 1979. DOI:10.1002/bimj.19700120316.
27. Trebak F.Alaoui A.Alexandre D. et al. Impact of aflatoxin B1 on hypothalamic neuropeptides regulating feeding behavior. Neurotoxicology. 2015;49(7):165–73. Available from: DOI: 10.1016/j.neuro.2015.06.008.
28. Hassan AA. Rashid MA.Koratum K. Effect Of Aflatoxin B1, Zearalenone And Ochratoxin A On Some Hormones Related To Fertility In Male Rats. Life Sci J. 2010;7(3):64–72. Available from: https://xueshu.baidu.com/usercenter/paper/show?paperid=a0d5efc58bd49a910a627015888dd354
29. Castelino JM.Routledge MN. Wilson S. et al. Aflatoxin exposure is inversely associated with IGF1 and IGFBP3 levels in vitro and in Kenyan schoolchildren. MolNutr Food Res. 2015;59(3):574–81. DOI: 10.1002/mnfr.201300619.
30. Rasheed N.Alghasham A.Rasheed Z. Lactoferrin from Camelusdromedarius Inhibits Nuclear Transcription Factor-kappa B Activation, Cyclooxygenase-2 Expression and Prostaglandin E2 Production in Stimulated Human Chondrocytes. Pharmacognosy Res. 2016;8(2):135–41. DOI: 10.4103/0974-8490.175612.
31. Rasheed Z. Medicinal values of bioactive constituents of camel milk: A concise report. Int J Health Sci (Qassim). 2017;11(5):1–2. PMID: 29114185; PMCID: PMC5669503.
32. Muleta A. Hailu D. Stoecker BJ. Belachew T. Camel milk consumption is associated with less childhood stunting and underweight than bovine milk in rural pastoral districts of Somali, Ethiopia: a cross-sectional study. Journal of Nutritional Science, 2021; 10(9), 1–8. DOI: 10.1017/JNS.2021.75.
33. Supriya C.Girish BP.Sreenivasula Reddy P. Aflatoxin B1-induced reproductive toxicity in male rats: Possible mechanism of action. Int J Toxicol. 2014;33(3):155–61. DOI: 10.1177/1091581814530764.
34. Dohle GR. Smit M. Weber RFA. Androgens and male fertility. World Journal of Urology, 2003; 21(5), 341–345. DOI: 10.1007/S00345-003-0365-9.
35. Ebaid H. Abdel-Salam B. Hassan I. et al. Camel milk peptide improves wound healing in diabetic rats by orchestrating the redox status and immune response. Lipids Health Dis. 2015;14(1):132. DOI: 10.1186/s12944-015-0136-9.
36. El-Sawy HBI. Soliman MM. El-Shazly SA. Et al. Protective effects of camel milk and vitamin E against monosodium glutamate induced biochemical and testicular dysfunctions. ProgrNutr. 2018; 20(1):76-85. DOI: 10.23751/pn.v20i1.5870.
37. Owumi SE.Adedara IA.Akomolafe AP. Et al. Gallic acid enhances reproductive function by modulating oxido-inflammatory and apoptosis mediators in rats exposed to aflatoxin-B1. ExpBiol Med. 2020;245(12):1016–28. DOI: 10.1177/1535370220936206.
38. Mohamed AAR.Abdellatief SA.Khater SI. Ali H. Al-Gabri NA. Fenpropathrin induces testicular damage, apoptosis, and genomic DNA damage in adult rats: Protective role of camel milk. Ecotoxicol Environ Saf. 2019;181(10):548–58. DOI: 10.1016/j.ecoenv.2019.06.047.
39. Mekuria AN.Routledge MN. Gong YY.Sisay M. Aflatoxins as a risk factor for liver cirrhosis: A systematic review and meta-analysis. BMC PharmacolToxicol. 2020;21(1):1–8. Available from: DOI: 10.1186/s40360-020-00420-7.
40. Korish AA.Arafah MM. Camel milk ameliorates steatohepatitis, insulin resistance and lipid peroxidation in experimental non-alcoholic fatty liver disease. BMC Complement Altern Med. 2013;13:264. DOI: 10.1186/1472-6882-13-264.
41. Zuberu J.Saleh MIA.Alhassan AW. Et al. Hepatoprotective Effect of Camel Milk on Poloxamer 407 Induced HyperlipidaemicWistar Rats. Open Access Maced J Med Sci. 2017;5(7):852–8. DOI: 10.3889/oamjms.2017.158.
42. Li H. Xing L. Zhang M. Wang J.Zheng N. The Toxic Effects of Aflatoxin B1 and Aflatoxin M1 on Kidney through Regulating L-Proline and Downstream Apoptosis. Biomed Res Int. 2018;(8):9074861. DOI: 10.1155/2018/9074861.
43. Al-Asmari AK.Abbasmanthiri R. Al-Elawi AM. Et al. Effect of camel milk against renal toxicity in experimental rats. Pak J Pharm Sci. 2017;30(2):561–5. https://pubmed.ncbi.nlm.nih.gov/28650321/.