Author(s):
Pradeep Kumar Samal, Bharti Vaishnaw, Bharti Ahirwar, Kedar Prasad Meena, Aarti Tiwari, Kamdev Sen, Dipendra Nirmalkar, Pameshwar Sahu, Geetanjali Darsena
Email(s):
samalpharmacology@rediffmail.com
DOI:
10.52711/0974-360X.2023.00977 
Address:
Pradeep Kumar Samal*, Bharti Vaishnaw*, Bharti Ahirwar, Kedar Prasad Meena, Aarti Tiwari, Kamdev Sen, Dipendra Nirmalkar, Pameshwar Sahu, Geetanjali Darsena
Guru Ghasidas Vishwavidyalaya, Bilaspur, Chhattisgarh.
*Corresponding Author
Published In:
Volume - 16,
Issue - 12,
Year - 2023
ABSTRACT:
Objective: To study the Hepatoprotective potential and Antioxidant activity of 4 – hydroxybenzyl alcohol in carbon tetrachloride induced liver damage in rats. Materials and methods: Rats were divided into five groups of six animals. Group I was treated with normal saline (Normal control) Group II was treated with the CCl4 hepatotoxic (negative control) Group III was treated with standard drug silymarin (positive control) and Group IV and V was treated with test drug 4–hydroxybenzyl alcohol (Test groups) respectively. On the 7th day, rats were sacrificed and blood was withdrawn by cardiac puncture. The levels and activities of serum biochemical parameters and antioxidant enzymes were then assayed using standard procedures. Results: The hepatoprotective activity of 4–hydroxybenzyl alcohol in this study was compared with the reference drug silymarin. In CCl4 treated animals, 4–hydroxybenzyl alcohol significantly decreased levels of serum transaminases, alkaline phosphatase and total bilirubin, and increased the level of total protein. Treatment with 4 – hydroxybenzyl alcohol resulted in a significant increase in the levels of catalase, superoxide dismutase and reduced glutathione as compared to CCl4 treated group. Histopathology of the liver tissue further supports the biochemical findings confirming the hepatoprotective potential of 4 – hydroxybenzyl alcohol. Conclusions: From the results it can be concluded that 4–hydroxybenzyl alcohol possesses Hepatoprotective effect and antioxidant activity against CCl4 induced liver damage in rats.
Cite this article:
Pradeep Kumar Samal, Bharti Vaishnaw, Bharti Ahirwar, Kedar Prasad Meena, Aarti Tiwari, Kamdev Sen, Dipendra Nirmalkar, Pameshwar Sahu, Geetanjali Darsena. Evaluation of the Hepatoprotective potential and Antioxidant activity of 4–Hydroxybenzyl alcohol on Carbon tetrachloride Induced liver damage in Experimental animals. Research Journal of Pharmacy and Technology.2023; 16(12):6022-7. doi: 10.52711/0974-360X.2023.00977
Cite(Electronic):
Pradeep Kumar Samal, Bharti Vaishnaw, Bharti Ahirwar, Kedar Prasad Meena, Aarti Tiwari, Kamdev Sen, Dipendra Nirmalkar, Pameshwar Sahu, Geetanjali Darsena. Evaluation of the Hepatoprotective potential and Antioxidant activity of 4–Hydroxybenzyl alcohol on Carbon tetrachloride Induced liver damage in Experimental animals. Research Journal of Pharmacy and Technology.2023; 16(12):6022-7. doi: 10.52711/0974-360X.2023.00977 Available on: https://rjptonline.org/AbstractView.aspx?PID=2023-16-12-78
REFERENCES:
1. Mroueh M, Saab Y, Rizkallah R. Hepatoprotective activity of Centaurium erythraea on acetaminophen-induced hepatotoxicity in rats. Phyther Res. 2004; 18(5): 431-433. doi:10.1002/ptr.1498
2. Hepatotoxicity D related. Drug-Related Hepatotoxicity. Published online 2006:731-739.
3. Venkatesh P, D HK, Dinakar A. Hepatoprotective Activity of an Aqueous Extract of Stem and Leaves of Boerhaavia diffusa Against Carbon Tetra Chloride Induced Hepatotoxicity in Rats. 2010; 3(3): 840-842.
4. Halliwell B. Oxygen radicals as key mediators in neurological disease: Fact or fiction? Ann Neurol. 1992; 32(1S): S10-S15. doi:10.1002/ana.410320704
5. Gutteridge JMC. Iron and oxygen radicals in brain. Ann Neurol. 1992; 32(1S): S16-S21. doi:10.1002/ana.410320705
6. Zachariah SM, Aleykutty N, Viswanad V, Halima OA. An overview on hepatoprotective activity of natural products. Res J Pharm Technol. 2012; 5(3):317-321.
7. Ames BN, Shigenaga MK, Hagen TM. Oxidants, antioxidants, and the degenerative diseases of aging. Proc Natl Acad Sci U S A. 1993; 90(17): 7915-7922. doi:10.1073/pnas.90.17.7915
8. Prior RL. Fruits and vegetables in the prevention of cellular oxidative damage. Am J Clin Nutr. 2003; 78(3 SUPPL.):570-578. doi:10.1093/ajcn/78.3.570s
9. Weisburger JH. Mechanisms of action of antioxidants as exemplified in vegetables, tomatoes and tea. Food Chem Toxicol. 1999; 37(9-10): 943-948. doi:10.1016/S0278-6915(99)00086-1
10. Chakraborty P, Kumar S, Dutta D, Gupta V. Role of Antioxidants in Common Health Diseases. Res J Pharm Tech. 2009; 2(2): 238-244.
11. Samal PK. Evaluation of antioxidant activity of Ardisia solanacea in albino rats. Res J Pharm Technol. 2013; 6(5): 585-588.
12. Bhathal PS, Rose NR, Mackay IR, Whittingham S. Strain differences in mice in carbon tetrachloride-induced liver injury. Br J Exp Pathol. 1983; 64(5): 524-533.
13. Komal Jain CM. Hepatoprotective Activity of Andrographolide and its Semi-Synthetic Derivatives. Res J Pharm Technol. 2022; 15(9).
14. Kuamwat RS, Mruthunjaya K, Gupta MK. Hepatoprotective effect of gallic acid and gallic acid phytosome against carbon tetrachloride induced damage in albino rats. Res J Pharm Technol. 2012; 5(5): 677-681.
15. Niha Naveed, Karthikeyan Murthykumar, Subasree Soundarajan SS. The Use of Neem in Oral Health. Res J Pharm Tech. 2014;. 7(9): 1060-1064.
16. Lettéron P, Labbe G, Degott C, et al. Mechanism for the protective effects of silymarin against carbon tetrachloride-induced lipid peroxidation and hepatotoxicity in mice. Evidence that silymarin acts both as an inhibitor of metabolic activation and as a chain-breaking antioxidant. Biochem Pharmacol. 1990; 39(12): 2027-2034. doi:10.1016/0006-2952(90)90625-U
17. Matkowski A. Plant in vitro culture for the production of antioxidants - A review. Biotechnol Adv. 2008; 26(6): 548-560. doi:10.1016/j.biotechadv.2008.07.001
18. Recknagel RO, Glende EA, Dolak JA, Waller RL. Mechanisms of carbon tetrachloride toxicity. Pharmacol Ther. 1989;43(1):139-154. doi:10.1016/0163-7258(89)90050-8
19. Glenn I. Nature of the Protection Tetrachloride-Induced Hepatotoxicity Produced By Pretreatment With Dibenamine. 1974; 23: 1479-1491.
20. Samal PK, Dangi JS, Meena KP, Beck NR, Maheshwari G, Patel A. Hepatoprotective activity of Butea monosperma bark on liver damage caused by paracetamol in rats. Res J Pharm Technol. 2011; 4(5): 771-774.
21. Samal PK. www.asianpharmaonline.org Investigation of Antiepileptic Activity of Hibiscus vitifolius leaves. 2013; 3(2): 63-66.
22. Aldhafiri FK, Santhanaraj B, Selvam G, et al. Hepatoprotective studies of methanolic leaf extract of Barringtonia acutangula (L) against carbon tetrachloride induced liver injury in wistar rats. Res J Pharm Technol. 2017; 10(1): 101-107. doi:10.5958/0974-360X.2017.00024.5
23. Reitman S, Frankel S. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am J Clin Pathol. 1957; 28(1): 56-63. doi:10.1093/ajcp/28.1.56
24. KIND PR, KING EJ. Estimation of plasma phosphatase by determination of hydrolysed phenol with amino-antipyrine. J Clin Pathol. 1954; 7(4): 322-326. doi:10.1136/jcp.7.4.322
25. Malloy HT, Evelyn KA. the Determination of Bilirubin With the Photoelectric Colorimeter. J Biol Chem. 1937; 119(2): 481-490. doi:10.1016/s0021-9258(18)74392-5
26. Misra HP, Fridovich I. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem. 1972; 247(10): 3170-3175. doi:10.1016/s0021-9258(19)45228-9
27. Góth L. A simple method for determination of serum catalase activity and revision of reference range. Clin Chim Acta. 1991; 196(2-3): 143-151. doi:10.1016/0009-8981(91)90067-M
28. Stehbens WE. Oxidative stress, toxic hepatitis, and antioxidants with particular emphasis on zinc. Exp Mol Pathol. 2003; 75(3): 265-276. doi:10.1016/S0014-4800(03)00097-2
29. Suryawanshi A, Joshi Y, Jain A, Kadam V. Protective Effect of Hexane Extract of Trachyspermum ammi Linn in Carbon Tetra Chloride-Induced Hepatitis in Rats. Res J Pharm Technol. 2015; 8(10): 1412. doi:10.5958/0974-360x.2015.00254.1
30. F.E. W. Tests in liver and biliary tract disease. CRC Press. Published online 1988: 567.
31. Drotman RB, Lawhorn GT. Serum enzymes as indicators of chemically induced liver damage. Drug Chem Toxicol. 1978; 1(2): 163-171. doi:10.3109/01480547809034433
32. NK SBSACBAKSOSKS. Hepatoprotective activity of verbenalin on experimental liver damage in rodents. Fitoterapia. 1998; 69(2): 15-140.
33. Rao RR. Alterations, As : 1973;5:313-318.
34. Clawson GA. Mechanisms of carbon tetrachloride hepatotoxicity. Pathol Immunopathol Res. 1989; 8(2): 104-112. doi:10.1159/000157141