Author(s): Naveen Kumari K, Srikanth Jeyabalan, Jayaraman Rajangam, N. Gopinathan, S.R. Ramakrishnan, V. Jayashankar reddy


DOI: 10.52711/0974-360X.2023.00965   

Address: Naveen Kumari K1, Srikanth Jeyabalan2*, Jayaraman Rajangam3, N. Gopinathan4, S.R. Ramakrishnan5, V. Jayashankar reddy6
1Research Scholar, Sri Ramachandra Institute of Higher Education and Research (DU), Porur, Chennai - 600116, Tamilnadu, India.
2Department of Pharmacology, Faculty of Pharmacy, Sri Ramachandra Institute of Higher Education and Research (DU), Porur, Chennai - 600116, Tamilnadu, India.
3Principal and Professor, Shri Venkateshwara College of Pharmacy, Ariyur, Pondicherry, India.
4Professor, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Dr. M.G.R Educational and Research Institute, Chennai - 600116, Tamilnadu, India.
5Professor/Senior Consultant, Department of Medicine, Sri Ramachandra Institute of Higher Education and Research (DU), Porur, Chennai.
6Vice-Principal and Head, Department of Pharmacology, Krishna Teja Pharmacy College, Chadalawada Nagar, Tirupati - 517506, Andhra Pradesh, India.
*Corresponding Author

Published In:   Volume - 16,      Issue - 12,     Year - 2023

Alzheimer's disease (AD) is mainly general form of dementia that is linked to the increase of extracellular amyloid beta (Aß) plaques. Oxidative stress and neurotoxicity events that are linked to the aetiology of AD have been postulated to be influenced by genetic, environmental, and dietary variables. Using the use of in vitro models, we attempted to determine whether or not Ulva lactuca had antioxidant and neuroprotective properties. SH-SY5Y The research employed pre-existing Neuroblastoma cell lines. Caspase 3 expression was also determined to know the level of protection of neuronal cells. Our results showed that the total extract of UL effectively reduced cell death and caspase 3 levels were also decreased in the cells treated with TEUL. The extract also has effective antioxidant properties proved. Further, it was also proved that the extract also has acetylcholinesterase enzyme inhibitory activity which is essential in treating AD.

Cite this article:
Naveen Kumari K, Srikanth Jeyabalan, Jayaraman Rajangam, N. Gopinathan, S.R. Ramakrishnan, V. Jayashankar reddy. Neuroprotective Potential of Total Extract of Ulva Lactuca: An In vitro study. Research Journal of Pharmacy and Technology.2023; 16(12):5948-3. doi: 10.52711/0974-360X.2023.00965

Naveen Kumari K, Srikanth Jeyabalan, Jayaraman Rajangam, N. Gopinathan, S.R. Ramakrishnan, V. Jayashankar reddy. Neuroprotective Potential of Total Extract of Ulva Lactuca: An In vitro study. Research Journal of Pharmacy and Technology.2023; 16(12):5948-3. doi: 10.52711/0974-360X.2023.00965   Available on:

1.    Lopes, F. M., Londero, G. F., de Medeiros, L. M., da Motta, L. L., Behr, G. A., de Oliveira, V. A., Ibrahim, M., Moreira, J. C., Porciúncula, L. O., da Rocha, J. B., and Klamt, F. Evaluation of the neurotoxic/neuroprotective role of organoselenides using differentiated human neuroblastoma SH-SY5Y cell line challenged with 6-hydroxydopamine. Neurotoxicity Research. 2012; 22(2): 138–149.
2.    Silva, J., Alves, C., Pinteus, S. et al. Neuroprotective effects of seaweeds against 6-hydroxidopamine- induced cell death on an in vitro human neuroblastoma model. BMC Complement Altern Med. 2018; 18: 58.
3.    Jantas, D., Chwastek, J., Malarz, J., Stojakowska, A., and Lasoń, W. Neuroprotective Effects of Methyl Caffeate against Hydrogen Peroxide-Induced Cell Damage: Involvement of Caspase 3 and Cathepsin D Inhibition. Biomolecules. 2020; 10(11): 1530.
4.    Tian W, Zhao J, Lee J-H, Akanda MR, Cho J-H, Kim S-K, Choi Y-J, Park B-Y. Neuroprotective Effects of Cornusofficinalis on Stress-Induced Hippocampal Deficits in Rats and H2O2-Induced Neurotoxicity in SH-SY5Y Neuroblastoma Cells. Antioxidants. 2020; 9(1): 27.
5.    Esteban-Fernández A, Rendeiro C, Spencer JPE, del Coso DG, de Llano MDG, Bartolomé B and Moreno-Arribas MV. Neuroprotective Effects of Selected Microbial-Derived Phenolic Metabolites and Aroma Compounds from Wine in Human SH-SY5Y Neuroblastoma Cells and Their Putative Mechanisms of Action. Front. Nutr. 2017; 4:3.
6.    Joana Ferreira, Sara Santos, Helena Pereira. In Vitro Screening for Acetylcholinesterase Inhibition and Antioxidant Activity of Quercussuber Cork and Corkback Extracts. Evidence-Based Complementary and Alternative Medicine. 2020, Article ID 3825629, 8 pages, 2020.
7.    Adam Kostelnik, Alexander Cegan, Miroslav Pohanka. Acetylcholinesterase Inhibitors Assay Using Colorimetric pH Sensitive Strips and Image Analysis by a Smartphone. International Journal of Analytical Chemistry. 2017, Article ID 3712384, 8 pages, 2017.
8.    Levi P. Machado, Luciana R. Carvalho, Maria Cláudia M. Young, Elaine M. Cardoso-Lopes, Danilo C. Centeno, Leonardo Zambotti-Villela, PioColepicolo, Nair S. Yokoya, Evaluation of acetylcholinesterase inhibitory activity of Brazilian red macroalgae organic extracts. Revista Brasileira de Farmacognosia. 2015; 25(6): 657-662,
9.    Singh, M., and Ramassamy, C. In vitro screening of neuroprotective activity of Indian medicinal plant Withaniasomnifera. Journal of Nutritional Science. 2017; 6: e54.
10.    Chien-Min Lin, Yi-Tzu Lin, Tai-Lin Lee, ZuhaImtiyaz, Wen-Chi Hou, Mei-Hsien Lee,In vitro and in vivo evaluation of the neuroprotective activity of UncariahirsutaHaviland, Journal of Food and Drug Analysis. 2020; 28(1): 147-158
11.    Ashok Kumar Popuri, Prashanti Guttikonda. Sequestering Nickel(II) Ions from Aqueous Solutions using Ulva lactuca as a Low Cost Sorbent. Research J. Pharm. and Tech. 2016; 9(1): 49-52.
12.    Thilagar Gobinath, Kannan Kaviya, Samuthirapandian Ravichandran. Chitosan/Ulva lactuca polysaccharide Hydrogel containing copper (II) beneficial for Biofilm-associated wound infection: Formulation Characterizations and in vitro study. Research J. Pharm. and Tech. 2020; 13(9): 4224-4230.
13.    D. Santhosha. Pharmacognostic Study, Phytochemical Screening and Neuroprotective Activity of Methanolic extract of Gynandropsis gynandra against Transient Global Ischemia induced brain damage in rats. Asian J. Res. Pharm. Sci. 2019; 9(3): 156-162.
14.    Abhishek Singhal, Rahul V Takawale, Vaishali R Undale. Preventive neuroprotective effect of commonly used antihypertensive drugs in ischemic cerebral injury in rats. Research Journal of Pharmacology and Pharmacodynamics. 2015; 7(1): 11-18. 5836.2015.00003.8
15.    Rajesh Kumar Reddy P, Saravanan J and Praveen T K. Evaluation of Neuroprotective Activity of Melissa officinalis in MPTP Model of Parkinson’s Disease in Mice. Research J. Pharm. and Tech. 2019; 12(5): 2103-2108.
16.    Santosh Kumar Panda, A. Venkateshwar Reddy, Mohammad Shamim Qureshi. Antiepileptogenic and Neuroprotective effects of Ziziphus jujuba leaves methanolic extract against pentylenetetrazole-induced kindling model in mice. Research J. Pharm. and Tech. 2018; 11(1): 259-266.
17.    Vasudev Pai, C. S Shreedhara, Chandrashekar K. S, Aravinda Pai, Venkatesh Kamath. Cognitive Enhancement and Neuroprotective Effects of Ancient Ayurvedic Medicinal Plant Celastrus Paniculatus: An Overview. Research J. Pharm. and Tech. 2016; 9(8): 1295-1298. 360X.2016.00246.8
18.    Nagaraju Bandaru, Chandrasekhar Komavari, Uma Sankar Gorla, GSN Koteswarao, Umasankar Kulandaivelu, A. Ankarao. Neuroprotective effect of Conessinin on Elevated oxidative stress induced Alzheimers’disease in rats. Research J. Pharm. and Tech. 2020; 13(6): 2703-2707.
19.    Sree Lekshmi. R. S, P. Shanmugasundaram. Neuroprotective Properties of Statins. Research J. Pharm. and Tech. 2018; 11(8): 3581-3584.
20.    S. Karthika, N. Kannappan, TNK Suriyaprakash. Effect of Medicinal plants on amyloid β1-42 Intoxicated SH-SY5Y cell Lines - As Neuroprotective Evaluation. Research J. Pharm. and Tech. 2020; 13(7): 3351-3355.
21.    Narahari Narayan Palei, S. Ramu, V. Vijaya, K. Thamizhvanan, Anna Balaji. Green synthesis of silver nanoparticles using leaf extract of Lantana camara and its antimicrobial activity. International Journal of Green Pharmacy. 2020; 14(1): 1-7.
22.    Ramu Samineni, Jithendra Chimakurthy. Effect of Coformers on Novel Co-Crystals of Gabapentin: An in vivo Approach. Journal of Pharmaceutical Sciences and Research. 2018; 12(5): 639-648.
23.    Ramu Samineni, M Anil Kumar, J Malisha, K Pavani, K Bhavyasree, E R Nithin. Formulation and Evaluation of Topical Solid Lipid Nanoparticulate System of Aceclofenac. Int J Pharma Res Health Sci. 2018; 6(4): 2647-2650. issue4-2018-MS-15637.pdf
24.    Ramu Samineni, Jithendra Chimakurthy, K Sumalatha, G Dharani, J Rachana, K Manasa, P Anitha. Co- crystals: a review of recent trends in co crystallization of BCS class II drugs. Research Journal of Pharmacy and Technology. 2019; 12(7): 3117-3124.
25.    Ramu Samineni, Jithendra Chimakurthy, K Udayaratna, K Devatulasi, B Varshitha Reddy, G Keerthi Sri, Ager Deng Goc. A Quick Overview of Nano medicine Applications in Breast Cancer Detection, Imaging, and Therapy. Asian Journal of Advances in Medical Science. 2022; 4(2): 44-56.

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

56th percentile
Powered by  Scopus

SCImago Journal & Country Rank

Recent Articles


Not Available