Author(s):
S. Hemaa, P. Sankarganesh, V. Parthasarathy, R. Anbarasan
Email(s):
parthu0406@gmail.com
DOI:
10.52711/0974-360X.2023.00905 
Address:
S. Hemaa1, P. Sankarganesh2, V. Parthasarathy3*, R. Anbarasan4
1Department of Microbiology, Hindustan College of Arts and Science, Padur, Chennai - 603103, TN, India.
2Department of Food Technology, Hindustan Institute of Technology and Science, Padur, Chennai - 603103, TN, India.
3Department of Physics, Rajalakshmi Institute of Technology, Poonamalle, Chennai - 600124, Tamil Nadu, India
4Department of Product Development, School of Chemistry, SIMATS-SSE, Thandalam, Tamilnadu, 602105, India.
*Corresponding Author
Published In:
Volume - 16,
Issue - 12,
Year - 2023
ABSTRACT:
The isolated crown galls (a bacterial plant disease) from Pongamia pinnata leaf were investigated to identify the causative agent. The SEM analysis revealed that the causative agent in crown gall appeared to be a slender, pink and rod-shaped bacterium. The morphological, cultural, biochemical and tumorogenesis analyses were performed to identify causative organisms in the crown gall disease. The isolate was negative for Indole, MR and VP tests, but positive for Citrate, TSI, Urease, Catalase and Oxidase tests. The causative organism also developed a tumor-like sport during the potato disc assay after 3rd week of incubation. The causative agent in crown gall was identified as Agrobacterium tumefaciens. The ethanolic extract of Allium sativum produced an inhibitory zone of 15 mm against this isolate. The current study proved that ethanolic extract of garlic can be effectively used to treat crown gall disease in the economically valuable dicot plant of Pongamia pinnata.
Cite this article:
S. Hemaa, P. Sankarganesh, V. Parthasarathy, R. Anbarasan. Determination of the Anti-bacterial potency of Allium sativum (Garlic) extract against Agrobacterium tumefaciens causing Crown Gall Disease. Research Journal of Pharmacy and Technology. 2023; 16(12):5597-2. doi: 10.52711/0974-360X.2023.00905
Cite(Electronic):
S. Hemaa, P. Sankarganesh, V. Parthasarathy, R. Anbarasan. Determination of the Anti-bacterial potency of Allium sativum (Garlic) extract against Agrobacterium tumefaciens causing Crown Gall Disease. Research Journal of Pharmacy and Technology. 2023; 16(12):5597-2. doi: 10.52711/0974-360X.2023.00905 Available on: https://rjptonline.org/AbstractView.aspx?PID=2023-16-12-6
REFERENCES:
1. Kerpen L, Niccolini L, Licausi F, van Dongen J.T., Weits, D.A. Hypoxic Conditions in Crown Galls Induce Plant Anaerobic Responses That Support Tumor Proliferation. Frontiers in Plant Science. 2019 10:56. https://doi.org/10.3389/fpls.2019.00056
2. Gohlke, J., Deeken, R. Plant responses to Agrobacterium tumefaciens and crown gall development. Frontiers in Plant Science. 2014; 5: 155. https://doi.org/ 10.3389/fpls.2014.00155
3. Daniel P, Hans, T.-C.,Monica, L.P., Doru, P., Constantin, B., Catherine, B. Agrobacterium tumefaciens: From Crown Gall tumors to genetic transformation. J. Physilogic. Moleculecular Plant Pathology. 2011; 76(2): 76-81. https://doi.org/ 10.1016/J.Pmpp.2011.06.004.
4. Soriful, I., Munsina, A., Atikur, R, Mostafizur, R., Mauluda, A., Firoz, A. Isolation of Agrobacterium tumefaciens strains from Crown Gall Samples of Dicot plants in Bangladesh. Current Research in Bacteriology. 2010; 3(1): 27-36. DOI: 10.3923/crb. 2010.27.36.
5. Naruto F., Fumika, S., Yutaka, N., Kishiko, N., Minoru, T., Nobuaki, M., Kayo, M., Youichi, T. Crown Gall of tobacco caused by Agrobacterium tumefaciens biovar 1 in tobacco fields. Journal of General Plant Pathology. 2004; 70(1):39-44, https://doi.org/10.1007/s10327-003-0088-1.
6. Shih, P.Y., Chou, S.J., Müller, C., Halkier, B.A., Deeken, R., Lai, E.M. Differential roles of glucosinolates and camalexin at different stages of Agrobacterium-mediated transformation. Molecular Plant Pathology. 2018; 19(8): 1956–70. doi: 10.1111/mpp.12672
7. Gowthami, Y., Chandrasehar, G., Sathya, T.N. Isolation of Agrobacterium tumefaciens strains from stem Gall disease on Manilkara zapota tree in Padappai, Tamil Nadu, India. Biotechnological Research. 2004; 1(3):156-159.
8. Bobbrala, V., Rao, G.S., Maduri, D.B., Naidu, K. C. Biocide Potentialities of Different Plant Methanolic Extracts Against Crown Gall Bacteria viz Agrobacterium tumefaciens. Biomedical and Pharmacology Journal. 2009; 2(1): 79-84.
9. Kado, C.I. 2002. Crown Gall, The Plant Health Instructor, https://doi.org/10.1094/PHI-I-2002-1118-01.
10. Sumathi, R., Pavni, S., Sivakumar, T. Antimicrobial Evaluation of Lipid Extract of Pongamia pinnata Leaves. Research J. Pharm. and Tech. 2009; 2(4): 714-718.
11. Manikannan, M., Arumugam, S., Rangasamy, B., Selvaraj J. Immune Stimulation effects of Pongamia pinnata extracts, an In vitro Analysis. Research J. Pharm. and Tech. 2020; 13(1): 308-312. doi: 10.5958/0974-360X.2020.00062.1
12. Padmini, R., Sitrarasi, R., Razia, M. Molecular Docking Studies of Bioactive Compounds from Allium sativum Against EML4-ALK Receptor. Research J. Pharm. and Tech. 2017; 10(11): 3741-3747. doi: 10.5958/0974-360X.2017.00679.5
13. Hussein, J., Hussein, Imad Hadi, H., Mohammed Y.H. A Review: Anti-microbial, Anti-inflammatory effect and Cardiovascular effects of Garlic: Allium sativum. Research J. Pharm. and Tech. 2017; 10(11): 4069-4078. doi: 10.5958/0974-360X.2017.00738.7
14. Lolok, N., Muthmainnah, H., Annah, M.I., Saleh, A., Yuliastri, W.O., Isrul, M. Antidiabetic Effect of the Combination of Garlic Peel Extract (Allium sativum) and Onion Peel (Allium cepa) in Rats with Oral-Glucose Tolerance Method. Research J. Pharm. and Tech. 2019; 12(5): 2153-2156. doi: 10.5958/0974-360X.2019.00357.3
15. Fatimah, A.J., Hameed, S.A.H. Chemical Datasets, Antioxidant, Free Radicals Scavenger activities estimate in Aqueous Garlic (Allium sativum) extract. Research Journal of Pharmacy and Technology. 2021; 14(10): 5157-2. doi: 10.52711/0974-360X.2021.00897
16. Lia, A., Emilia, G., Ninis, Y., Giftania, W., Sudjarwo. In vitro Antiplatelet Activities of Aqueous Extract of Garlic (Allium sativum) and black Garlic in Human Blood. Research Journal of Pharmacy and Technology. 2022; 15(4): 1579-2. doi: 10.52711/0974-360X.2022.00263
17. Keshav, S., Deepak Kumar, B. Effect of liquid biofertilizer with Herbal Insecticide Garlic (Allium sativum L.) on productivity of Gram (Cicer arietinum) and Infestation of Helicoverpa armigera (Hübner). Research J. Pharm. and Tech. 2020; 13(6): 2567-2572. doi: 10.5958/0974-360X.2020.00457.6
18. Manjula, R., Syed Abdul, H., Aravind Yaswanth, C., Gowtham, V., Padma, T. Formulation and Characterization of Cedrus deodara Oil Emulsion and studies on its activity against representative food and plant pathogens. Research J. Pharm. and Tech. 2019; 12(3): 1333-1337. doi: 10.5958/0974-360X.2019.00223.3
19. Parvathi, P. Garlic- A Golden Wonder. Research J. Pharm. and Tech. 2018; 11(1): 393-396. doi: 10.5958/0974-360X.2018.00072.0
20. Anand, P.P., Ramani, N. Dynamics of limited neoplastic growth on Pongamia pinnata (L.) (Fabaceae) leaf, induced by Aceria pongamiae (Acari: Eriophyidae). BMC Plant Biology. 2021; 21: 1. https://doi.org/10.1186/s12870-020-02777-7
21. Hamed, K. Characterization and Biocontrol of Crown Gall Disease in Jordan. Jordan Journal of Agricultural Sciences. 2006; 2(3): 265-272.
22. Wise, A. A., Liu, Z., Binns, A.N. Culture and Maintenance of Agrobacterium Strains. Agrobacterium Protocols. 2006; 3–14. https://doi.org/10.1385/1-59745-130-4:3
23. Raffaele, P., Aida, R., Fabio, M., Astolfo, Z. Physiological, biochemical and molecular analyses of an Italian collection of Agrobacterium tumefaciens strains. Europe. Journal of Plant Pathology. 2003; 109: 291–300. https://doi.org/ 10.1023/A:1023556108085
24. Hussain, A., Zia, M., Mirza, B. Cytotoxic and antitumor potential of Fagonia cretica L. Turkish Journal of Biology. 2007 31: 19-24.
25. Mounyr, B., Moulay, S., Saad, K. I. Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis. 2016; 6(2): 71-79. https://doi.org/10.1016/j.jpha.2015.11.005
26. Erican, A. Inhibition of Crown Gall Tumorigenesis with Plant Extracts. Journal of Pharmaceutical Biology. 2009; 47(5): 463-466, https://doi.org/10.1080/1388020090824873
27. Abdul, Q.S., Palash, C. M., Soriful, I., Mohammad, F.A. Identification of Virulent Agrobacterium tumefaciens Strains from some Dicotyledonous Plants in Bangladesh. Agriculturae Conspectus Scientificus. 2011; 76 (2): 147-152.
28. Spiers, A.G. Isolation and characterisation of Agrobacterium species. New Zealand Journal of Agricultural Research. 1979; 22(4): 631-639, https://doi.org/10.1080/00288233.1979.10417834
29. Suksomtip, M., Liu, P., Wood, D., Nester, E.W. Citrate synthase mutants of Agrobacterium are attenuated in virulence and display reduced vir gene induction. J. Bacteriology. 2005; 187: 4844–4852.
30. Nester, E.W. Agrobacterium: nature’s genetic engineer. Frontiers in Plant Sciences. 2015; 5: 730. https://doi.org/ 10.3389/fpls.2014.00730
31. Bosmans, L., Moerkens, R., Wittemans, L. De Mot, R., Rediers, H., Lievens, B. Rhizogenic agrobacteria in hydroponic crops: epidemics, diagnostics and control Plant Pathology. 2017; 66:1043. https://doi.org/10.1111/ppa.12687
32. Thompson, M.A., Onyeziri, M.C., Fuqua, C. Function and Regulation of Agrobacterium tumefaciens Cell Surface Structures that Promote Attachment. Curr. Topic. Microbiology and Immunology. 2018; 418: 143–184. https://doi.org/10.1007/82_2018_96
33. Aysan, et al. First report of Crown Gall of apricot (Preunus armenica) caused by Agrobacterium tumefaciens in Turkey. Journal of Plant Pathologys. 2003; 52(6): 793. https://doi.org/ 10.1111/j.1365-3059.2003.00902.x.
34. Rahman, S., Kafeel, A., Niaz A., Muhammad, I., Waqar, A. Isolation and Characterization of Agrobacterium tumefaciens Strains from Malakander Farm, University of Agriculture, Peshawar. Pakistan Journal of Zoology. 2018; 52(5): 2019-2021. https://dx.doi.org/10.17582/journal.pjz/20181021061044
35. Ganjeh, A., Heshmatollah, R., Esmaeil, B. Agrobacterium tumefaciens causing crown and stem gall disease of citrus propagation nursery in Iran. Journal of Plant Pathology. 2021; 103:355. https://doi.org/10.1007/s42161-020-00679-z