Author(s):
Ziad T I Alkayar, Ahmed A.Ismail, Nagham Muthanna Jassim Al-Ani, Fedoseenko Anastasia Alekseevna, Ahmed Mahdi Saeed, Haider Mahmoud Nasser Al-Amir, Mahmood Shakoori Yaseen, Bashaar Mohammed Tawfiq, Zena M. Jassem, Shaimaa Ahmad Hassan, Maryam O. Abd Ala
Email(s):
dr.shaimaa_altaee@kus.edu.iq
DOI:
10.52711/0974-360X.2023.00902 
Address:
Ziad T I Alkayar1*, Ahmed A.Ismail1, Nagham Muthanna Jassim Al-Ani2, Fedoseenko Anastasia Alekseevna3, Ahmed Mahdi Saeed1*, Haider Mahmoud Nasser Al-Amir2,Mahmood Shakoori Yaseen2, Bashaar Mohammed Tawfiq2, Zena M. Jassem4,Shaimaa Ahmad Hassan5, Maryam O. Abd Ala1
1Department of Chemistry, College of Education for Pure Science, University of Diyala, Iraq.
2The State Enterprise for Drugs Industries and Medical Appliances, Iraq.
3Emperor Alexander I St. Petersburg State Transport University, Russia.
4Ministry of Trade Iraq- grain board-quality Control Department, Iraq.
5College of Remote Sensing and Geophysics, Al Karkh University of Science, Baghdad, Iraq.
*Corresponding Author
Published In:
Volume - 16,
Issue - 12,
Year - 2023
ABSTRACT:
An effective, duplicable, and quick method to determine the purity and formulating form of Cetrimide (CE) have studied and modified utilizing the Reverse Phase High Performance Liquid Chromatography (RP-HPLC). The method was based on the separation of the CE using a reverse phase HPLC Shimadzu LC–20 A, Japan, and Phenomenex C18 column (15 × 4.6mm, 5.0µm). The process was carried out by using the mobile phase ammonium acetate solution 0.1M (AA), methanol (MeOH), and the methyl cyanide in the ratio of (15:60:25 V/V/V), a flow rate of 1.0mL/min, at pH = 3, with 10µL sample loop and the UV - spectrophotometry detector was set at 280nm. However, for the optimum conditions the mobile phase composition, wavelength, pH, and flow rate were studied. The calibration carve has obtained in the range of 10-1000mg/L and the correlation coefficient is 0.9985. The average recovery of the standard drug was 97.56%. The LOD was 5mg/L and LOQ is 16.5mg/L for CE. The process was used successfully to determine the CE in the pharmaceutical forms with the average recovery of 95.61 and 96.02%.
Cite this article:
Ziad T I Alkayar, Ahmed A.Ismail, Nagham Muthanna Jassim Al-Ani, Fedoseenko Anastasia Alekseevna, Ahmed Mahdi Saeed, Haider Mahmoud Nasser Al-Amir, Mahmood Shakoori Yaseen, Bashaar Mohammed Tawfiq, Zena M. Jassem,Shaimaa Ahmad Hassan, Maryam O. Abd Ala. Reverse Phase Liquid Chromatography for Cetrimide Determination in Pure and Pharmaceutical PreparationsResearch Journal of Pharmacy and Technology. 2023; 16(12):5581-5. doi: 10.52711/0974-360X.2023.00902
Cite(Electronic):
Ziad T I Alkayar, Ahmed A.Ismail, Nagham Muthanna Jassim Al-Ani, Fedoseenko Anastasia Alekseevna, Ahmed Mahdi Saeed, Haider Mahmoud Nasser Al-Amir, Mahmood Shakoori Yaseen, Bashaar Mohammed Tawfiq, Zena M. Jassem,Shaimaa Ahmad Hassan, Maryam O. Abd Ala. Reverse Phase Liquid Chromatography for Cetrimide Determination in Pure and Pharmaceutical PreparationsResearch Journal of Pharmacy and Technology. 2023; 16(12):5581-5. doi: 10.52711/0974-360X.2023.00902 Available on: https://rjptonline.org/AbstractView.aspx?PID=2023-16-12-3
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