Author(s): Mervat G. Hassan, Amr A. El-Waseif, Reem H. Abd El gawad, Omnia M. Arief, Sabah A. Abo El-Maaty


DOI: 10.52711/0974-360X.2023.00739   

Address: Mervat G. Hassan1, Amr A. El-Waseif2, Reem H. Abd El gawad1, Omnia M. Arief1, Sabah A. Abo El-Maaty1
1Botany and Microbiology Dept., Faculty of Science, Banha University, Egypt.
2Botany and Microbiology Dept., Faculty of Science (Boys), Al-Azhar University, Cairo, Egypt.
*Corresponding Author

Published In:   Volume - 16,      Issue - 10,     Year - 2023

The present article targeted to biosynthesis and description of copper oxide nanoparticles (CuO NPs) using probiotic isolate and assessment the antibacterial, cytotoxicity of these NPs and its effect on wound heals. The CuO NPs are biosynthesized using probiotic isolate identified as Bacillus cereus. These CuO NPs are characterized using UV, FT-IR, Zeta and TEM. The antibacterial efficiency is assayed by agar plate diffusion protocol. Results assured that the CuO NPs can prevent Staphylococcus aureus jointly Escherichia coli growth. MTT procedure exercised to detect cytotoxicity and wound healing effects. The results revealed that CuO NPs can use as antibacterial agent with non-significant effects on host cells and wound healing.

Cite this article:
Mervat G. Hassan, Amr A. El-Waseif, Reem H. Abd El gawad, Omnia M. Arief, Sabah A. Abo El-Maaty. Assessment of Antibacterial, Cytotoxicity and Wound Healing Influence of Copper Nanoparticles Synthesized using Probiotic Bacteria. Research Journal of Pharmacy and Technology 2023; 16(10):4537-2. doi: 10.52711/0974-360X.2023.00739

Mervat G. Hassan, Amr A. El-Waseif, Reem H. Abd El gawad, Omnia M. Arief, Sabah A. Abo El-Maaty. Assessment of Antibacterial, Cytotoxicity and Wound Healing Influence of Copper Nanoparticles Synthesized using Probiotic Bacteria. Research Journal of Pharmacy and Technology 2023; 16(10):4537-2. doi: 10.52711/0974-360X.2023.00739   Available on:

1.    El-Ghwas D., Mazeed T., El-Waseif A., Al-Zahrani H., AlmaghrabiO.andElazzazy A.. Factorial Experimental Design for Optimization of Zinc Oxide Nanoparticle Production. Current Nanoscience. 2020; 16(1):51-61. doi: 10.2174/1573413715666190618103127
2.    Kouhkan M., Ahangar P., Babaganjeh L. A., and Allahyari-Devin M. Biosynthesis of Copper Oxide Nanoparticles Using Lactobacillus casei Subsp. Casei and its Anticancer and Antibacterial Activities Current Nanoscience. 2020; 16 (1): 101-111. doi: 10.2174/1573413715666190318155801
3.    El-Ghwas D. E. and El-Waseif A. A. The Synthesis of Silver Nanoparticals from Streptomyces sp. with Antimicrobial Activity. International Journal of Pharm Tech Research. 2016; 9(4): 179-186.
4.    El-Waseif A. A., Gaber H. S., Ewais E. A. Hypocholesterolemic Operating Parameters of Novel Probiotics In vitro. Research J. Pharm. and Tech. 2021; 14(10): 5197-5201.doi: 10.52711/0974-360X.2021.00904
5.    El-Waseif A. A., Roshdy T. Y., Abdel-Monem M.O., Hassan M. G. Taguchi design Analysis for optimization of probiotics cholesterol assimilation. Materials Today: Proceedings. 2022; 61(3):  1154-1157.
6.    El-Waseif A. A., Abobaker R. A., Abdel-Monem M. O., Attia A. A., Hassan M. G. The Lactobacillus brevis Prebiotic Pure Exo polysaccharide and its Nano crystalline Characterization, anti-colon cancer and cytotoxicity. Research J. Pharm. and Tech. 2021; 14(11): 5998-6002.doi: 10.52711/0974-360X.2021.01042
7.    El-Waseif A. A. and El-Ghwas D. E. Involving the Silver Particles into Microbial Membrane to Improve the Biological Activity and Characterization. International Journal of Pharm Tech Research. 2016; 9(5):16-22.
8.    El-Waseif A. A., Eissa H. A., Abo El maaty S. A., Hassan M. G. Anti-Virulence and Cytotoxic Behavior of Copper Nanoparticles against antibiotics-resistant Staphylococcus aureus. Egyptian Journal of Chemistry. 2022; 65(9): 361–367.  10.21608/EJCHEM.2022.111832.5079
9.    El-Waseif A.A., Abd El-Ghani G. S., Abo El maaty S. A., Hassan M. G. Cytotoxicity and Promising Anti-Biofilm of Curcuma Silver Nanoparticles against Candida albicans. Research J. Pharm. and Tech. 2022; 15(8): 3355-3359.doi: 10.52711/0974-360X.2022.00561
10.    El-Waseif  A. A. and El-Ghwas D. E. Anti-Breast Cancer and Cytotoxicity of Nano Materials Formed Bacterial Cellulose-ZnO-Ag Composite.Materials Today: Proceedings. 2022; 60(3):  1651-1655.
11.    De Man, J.C., M. Rogosa, and E. Sharpe (1960). A medium for the cultivation of Lactobacilli. J. Appl. Bacteriol. 23:130-135.
12.    Al-Janabi SS., Shawky H, El-Waseif A A., Farrag A A., Abdelghany T M., El-Ghwas D E. (2022). Novel approach of amplification and cloning of bacterial cellulose synthesis (bcs) operon from Gluconoacetobacterhansenii. Gene Reports. 27(2): 101577.
13.    Humphries R, Ambler J, Mitchell SL, Castanheira M, Dingle T, Hindler JA, Koeth L, Sei K, Hardy D, Zimmer B, et al. (2018). CLSI methods development and standardization working group best practices for evaluation of antimicrobial susceptibility tests. J. Clin. Microbiol. 56: 1917-1934.
14.    Wayne P. (2013). Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Third Informational Supplement. M100-S23.  
15.    Senthilraja P. Kathiresan K. In vitro cytotoxicity MTT assay in Vero, HepG2 andMCF-7 cell lines study of marine yeast. J Appl Pharm Sci. 2015; 5:80–84.
16.    Lee NK, Kim WS, Paik HD. Bacillus strains as human probiotics: characterization, safety, microbiome, and probiotic carrier. Food Sci Biotechnol. 2019 (8);28(5):1297-1305. PMID: 31695928; PMCID: PMC6811671.doi: 10.1007/s10068-019-00691-9.
17.    Vidovix T. B., H. B. Quesada, E. F. DiogoJanuário, R. Bergamasco, A. M. Salcedo Vieira, Green synthesis of copper oxide nanoparticles using Punicagranatum leaf extract applied to the removal of methylene blue, Materials Letters. 257; 2019, 126685,
18.    Sangeetha G., Sivaraj R., Venckatesh R., Aloe barbadensis Miller mediated green synthesis of mono-disperse copper oxide nanoparticles: Optical properties, SpectrochimicaActa Part A: Molecular and Biomolecular Spectroscopy. 2012;  97: 1140-1144,
19.    Kim, J.H.; Cho, H.; Ryu, S.E.; Choi, M.U. Effects of metal ions on the activity of protein tyrosine phosphatase VHR: Highly potent and reversible oxidative inactivation by Cu2+ ion. Arch. Biochem. Biophys. 2000; 382(1): 72-80.
20.    TavassoliHojati, S.; Alaghemand, H.; Hamze, F.; AhmadianBabaki, F.; Rajab-Nia, R.; Rezvani, M.B.; Kaviani, M.; Atai, M. Antibacterial, physical and mechanical properties of flowable resin composites containing zinc oxide nanoparticles. Dent. Mater. 2013; 29(5): 495-505.
21.    Sondi, I.; Salopek-Sondi, B. Silver nanoparticles as antimicrobial agent: A case study on E. coli as a model for gram-negative bacteria. J. Colloid Interface Sci., 2004, 275(1), 177-182.
22.    Pazos-Ortiz, E.; Roque-Ruiz, J.H.; Hinojos-Marquez, E.A.; Lopez- Esparza, J.; Donohue-Cornejo, A.; Cuevas-Gonzalez, J.C.; Espinosa- Cristobal, L.F.; Reyes-Lopez, S.Y. Dose-dependent antimicrobial activity of silver nanoparticles on polycaprolactone fibers against gram-positive and gram-negative bacteria. J. Nanomater. 2017; 2017, Article ID 4752314.
23.    Abbaszadegan, A.; Ghahramani, Y.; Gholami, A.; Hemmateenejad, B.; Dorostkar, S.; Nabavizadeh, M.; Sharghi, H. The effect of charge at the surface of silver nanoparticles on antimicrobial activity against gram-positive and gram-negative bacteria: A preliminary study. J. Nanomater. 2015; 2015, Article ID 720654.
24.    Khan, M.F.; Hameedullah, M.; Ansari, A.H.; Ahmad, E.; Lohani, M.B.; Khan, R.H.; Alam, M.M.; Khan, W.; Husain, F.M.; Ahmad, I. Flower-shaped ZnO nanoparticles synthesized by a novel approach at near-room temperatures with antibacterial and antifungal properties. Int. J. Nanomedicine. 2014; 9: 853-864.
25.    Sen, C.K., Khanna, S., Venojarvi, M., et al.: ‘Copper-induced vascular endothelial growth factor expression and wound healing’, Am. J. Physiol. Heart Circ. Physiol. 2002; 282:  1821–1827.
26.    Tiwari M, Narayanan K, Thakar MB, Jagani HV, VenkataRao J. Biosynthesis and wound healing activity of copper nanoparticles. IET Nanobiotechnol. 2014; 8(4): 230-7. PMID: 25429502. doi: 10.1049/iet-nbt.2013.0052.
27.    AbhirupDey, Mangala Lakshmi Ragavan, Sanjeeb Kumar Mandal, Nilanjana Das. Isolation, Identification and In vitro Characterisation of Probiotic Yeast Strains. Research J. Pharm. and Tech. 2017; 10(3): 726-732.
28.    HarshaAchanta, Suneetha V. Isolation and Biochemical Characterization of Probiotic Lactobacillus Species Isolated from Curd Samples of Southern Regions of Vellore, Tamil Nadu. Research J. Pharm. and Tech. 2017; 10(6): 1734-1741.
29.    Huda W. Hadi. Genetic study on Bacillus cereus. Research J. Pharm. and Tech. 2019; 12(3): 1301-1306.

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

56th percentile
Powered by  Scopus

SCImago Journal & Country Rank

Recent Articles


Not Available