Naveen Kumar, Pramod Kumar, Satheesha Nayak B, Melissa Glenda Lewis, Ashwini Aithal P, Ravi Bhaskar
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Naveen Kumar1, Pramod Kumar2, Satheesha Nayak B3, Melissa Glenda Lewis4, Ashwini Aithal P3, Ravi Bhaskar5
1Department of Anatomy, Ras Al Khaimah College of Medical Sciences, RAK Medical and Health Sciences University, Ras Al Khaimah, UAE (Present). Former Faculty of Melaka Manipal Medical College, Manipal Academy of Higher Education, Manipal, India.
2Consultant Plastic Surgeon, King Fahad Central Hospital, Jazan, Saudi Arabia.
3Division of Anatomy, Department of Basic Medical Sciences, Manipal Academy of Higher Education, Manipal, India.
4Indian Institute of Public Health, Shillong, Meghalaya, India.
5Department of Anatomy, Sapthagiri Institute of Medical Sciences and Research Centre, Bengaluru, India.
Volume - 16,
Issue - 10,
Year - 2023
Background: The collagen and the elastic fiber content of the dermis is known to show the asymmetric distribution across the planes of the body region. During the period of limb development, the upper and lower limbs rotate 90 degrees laterally and medially respectively. Objectives: The study aims to check if the limb bud rotation during embryonic development favors the unequal distribution of the dermal connective tissue fiber content distribution. Methods: A total of 240 skin samples from the forearm and thigh regions of adult human cadavers were collected in horizontal and vertical directions from the anatomical and oblique planes. The tissues were processed with tissue-quant analysis. The data were analyzed statistically using SPSS. Results: There were no significant differences in the pattern of distribution of the collagen and elastic fiber contents in the tissues of horizontal and vertical directions from the two planes of forearm and thigh regions. However significant Spearman's correlation was observed in both elements in the thigh region but only the collagen content in the forearm region. Conclusion: The pattern of distribution of connective tissue fibers in the dermis is regulated by the mechanical communications between the cells and their extracellular matrices. Hence, the significant role of embryologic limb rotation in the event of unequal distribution of dermal collagen and elastic fibers in the limb region is to be ruled out.
Cite this article:
Naveen Kumar, Pramod Kumar, Satheesha Nayak B, Melissa Glenda Lewis, Ashwini Aithal P, Ravi Bhaskar. Does the Embryologic Limb Rotation Modify the Distribution of Dermal Collagen and Elastic Fibers in Different Planes of Human Extremities?. Research Journal of Pharmacy and Technology 2023; 16(10):4525-9. doi: 10.52711/0974-360X.2023.00737
Naveen Kumar, Pramod Kumar, Satheesha Nayak B, Melissa Glenda Lewis, Ashwini Aithal P, Ravi Bhaskar. Does the Embryologic Limb Rotation Modify the Distribution of Dermal Collagen and Elastic Fibers in Different Planes of Human Extremities?. Research Journal of Pharmacy and Technology 2023; 16(10):4525-9. doi: 10.52711/0974-360X.2023.00737 Available on: https://rjptonline.org/AbstractView.aspx?PID=2023-16-10-7
1. Thomas S. Langmans Medical Embryology (9th edition). Lippincott Williams and Wilkins 2003, 111-187.
2. Marcos-Garcés V, Molina Aguilar P, Bea Serrano C, García BV, Benavent SJ, Ferrández I A, Ruiz-Saurí A. Age-related dermal collagen changes during development, maturation and ageing - a morphometric and comparative study. J Anat. 2014;225:98–108. doi: 10.1111/joa.12186.
3. Furth JJ, Allen RG, Tresini M, Keogh B, Cristofalo VJ. Abundance of α1(I) and α1(III) procollagen and p21 mRNAs in fibroblasts cultured from fetal and postnatal dermis. Mech Ageing Dev. 1997; 97:131–142. doi: 10.1016/s0047-6374(97)00051-1.
4. Branchet MC, Boisnic S, Frances C, Lesty C, Robert L. Morphometric analysis of dermal collagen fibers in normal human skin as a function of age. Arch Gerontol Geriatr. 1991; 13:1–14. doi: 10.1016/0167-4943(91)90011-e.
5. Frances C, Branchet MC, Boisnic S, Lesty C, Robert L. Elastic fibers in normal human skin. Variation with age: a morphometric analysis. Arch Gerontol Geriatr. 1990;10: 57–67. doi: 10.1016/0167-4943(90)90044-7.
6. Naveen K, Pramod K, Keerthana P, Satheesha B, Ranjini K, Raghuveer CV. Histomorphometric analysis of dermal collagen and elastic fibers in skin tissues taken perpendicular to each other from Head and Neck region. Journal of Surgical Academia 2014;4(1):30-36.
7. Kumar N, Kumar P, Badagabettu SN, Prasad K, Kudva R, Raghuveer CV. Quantitative fraction evaluation of dermal collagen and elastic fibres in the skin samples obtained in two orientations from the trunk region, Dermatol Res Pract. 2014; 2014:251254. doi: 10.1155/2014/251254.
8. Naveen K, Pramod K, Keerthana P, Ranjini K, Raghuveer CV. Surgical implications of asymmetric distribution of dermal collagen and elastic fibers in two orientations of skin samples from extremities. Plast Surg Int. 2014;2014:364573. doi: 10.1155/2014/364573.
9. Bancroft JD, Gamble M. Theory and Practice of Histological Techniques. 5th edition. London; UK: Churchill Livingstone; 2002:127–156.
10. Naveen K, Pramod K, Satheesha N, Ashwini AP, Anitha G. Ratio Analysis Evaluation of Dermal Collagen and Elastic Fiber Contents: A Comparison of the Horizontal and Vertical Directions of Skin Tissues. J Clin Aesthet Dermatol. 2019; 12:21–24.
11. Veerabhadrappa G Mendagudli, Shivaleela S Sarawad. Obesity and its Impact on Health. International Journal of Nursing Education and Research. 2021; 9(4):485-7. doi: 10.52711/2454-2660.2021.00113.
12. Cynthia LJ, Mark D. The influence of presumptive limb connective tissue on motoneuron axon guidance. Dev Biol. 1991; 143: 93-110. doi: 10.1016/0012-1606(91)90057-a.
13. Cao L, Lafyatis R, Burkly LC. Increased dermal collagen bundle alignment in systemic sclerosis is associated with a cell migration signature and role of Arhgdib in directed fibroblast migration on aligned ECMs. PLoS One. 2017; 12(6):e0180751. doi: 10.1371/journal.pone.0180751.
14. van Zuijlen PP, de Vries HJ, Lamme EN, Coppens JE, van Marle J, Kreis RW, Middelkoop E. Morphometry of dermal collagen orientation by Fourier analysis is superior to multi-observer assessment. J Pathol. 2002; 198(3):284-91. doi: 10.1002/path.1219.
15. S.K.Purohit, R. Solanki. Microorganisms Responsible for Wound Infection on Human Skin. Asian J. Res. Pharm. Sci. 2013; 3(2): 68-71. https://ajpsonline.com/AbstractView.aspx?PID=2013-3-2-4.
16. Shubhangi Dwivedi, Prashant Tiwari. Burn Wound: An update focusing the classification, Immune responses and management resources aid in healing. Research J. Science and Tech. 2014; 6(3):128-132. https://rjstonline.com/AbstractView.aspx?PID=2014-6-3-2.
17. Deependra Singh, Mukesh K Nag, Satish Patel, Madhulika Pradhan, Shikha Shrivastava, SJ Daharwal, Manju R Singh. Treatment Strategies in Burn Wounds: An Overview. Research J. Pharmacology and Pharmacodynamics. 2013; 5(6): 341-352. https://rjppd.org/AbstractView.aspx?PID=2013-5-6-17
18. Ridhanya, Rajakumari. K. Skin Wound Healing: An update on the Current knowledge and Concepts. Research J. Pharm. and Tech. 2019; 12(3): 1448-1452. doi: 10.5958/0974-360X.2019.00240.3.
19. Rohit Kumar, Reena Gupta, Bimlesh Kumar, Bhupinder Kapoor. Wound Healing Potential of Polyherbal Formulation in Rats. Research J. Pharm. and Tech. 2021; 14(4):2195-9. doi: 10.52711/0974-360X.2021.00389.