Author(s):
Seema BR, Jyothi. Y, Chetankumar N Bhovi, Vivek Aradhya M V, Mukesh Lekhak, Shweta R Mane
Email(s):
jokiran05@gmail.com
DOI:
10.52711/0974-360X.2023.00736 
Address:
Seema BR, Jyothi. Y*, Chetankumar N Bhovi, Vivek Aradhya M V, Mukesh Lekhak, Shweta R Mane.
Department of Pharmacology, Krupanidhi College of Pharmacy, Bangalore.
*Corresponding Author
Published In:
Volume - 16,
Issue - 10,
Year - 2023
ABSTRACT:
Curly kale is reported to contain powerful antioxidants like quercetin and kaempferol, and glucosinolates which is used to treat different diseases. Despite its favorable usage, there have been no investigations on its toxicity potential. According to OECD TG No.425, the study was aimed to investigate the acute toxic outcome of an aqueous extract of curly kale. Female wistar albino rats were randomly split into 2 groups (no=5). 1st group was used as the control group, while the 2nd group was used as the treated group and administered 2000mg/kg body weightof curly kale orally. Following that 2 groups were monitored for fourteen days. Then the blood samples were collected by cardiac puncture, and handover to haematological and biochemical analyses. The animal vital organs were stored for histopathological examination. The data disclose that the extract’s LD50 was more than 2000mg/Kg b.w. Body weight and organ to body mass ratio did not change significantly. Against the control group. But alkaline phosphates, total bilirubin, aspartate aminotransferase (ALT), Total protein, globulin, blood urea, cholesterol, and triglycerides, all increased dramatically, while total blood count, packed cell volume (PVC), mean corpuscular volume (MCV), corpuscular volume haemoglobin (MCH), and red blood cells (RBC) count all decreased significantly. Although the LD50 was larger than 2000 mg/kg body weight, mild toxicity indications developed in the liver, spleen, lipid profile, and complete blood count (CBC) also revealed blood dyscrasias at the maximum dose, according to the findings obtained in this investigation.
Cite this article:
Seema BR, Jyothi. Y, Chetankumar N Bhovi, Vivek Aradhya M V, Mukesh Lekhak, Shweta R Mane. Advanced up and down methodology for acute toxicity assessment with reliable LD50 verified by aqueous extract of curly kale using wistar rats. Research Journal of Pharmacy and Technology 2023; 16(10):4519-4. doi: 10.52711/0974-360X.2023.00736
Cite(Electronic):
Seema BR, Jyothi. Y, Chetankumar N Bhovi, Vivek Aradhya M V, Mukesh Lekhak, Shweta R Mane. Advanced up and down methodology for acute toxicity assessment with reliable LD50 verified by aqueous extract of curly kale using wistar rats. Research Journal of Pharmacy and Technology 2023; 16(10):4519-4. doi: 10.52711/0974-360X.2023.00736 Available on: https://rjptonline.org/AbstractView.aspx?PID=2023-16-10-6
REFERENCES:
1. Shenoy G, Shenoy S, Pai SB, Kumar N, Amuthan A, Shetty M, Rao M, Rao BK. Long term toxicity study of a Siddha formulation Vetpalaithailam (VT) in Rats. Research Journal of Pharmacy and Technology. 2021;14(12):6653-8.DOI : 10.52711/0974-360X.2021.01149
2. Vickers A, Zollman C. Herbal medicine. Bmj. 1999 Oct 16;319(7216):1050-3.doi: 10.1136/bmj.319.7216.1050.
3. Mukhi S, Bose A, Das DK, Panda SK, Mohapatra D, Latha S, Balaraman AK. Acute and sub-acute toxicity study of Amrtadichurna. Research Journal of Pharmacy and Technology. 2021;14(6):3111-8.DOI : 10.52711/0974-360X.2021.00543
4. Pal SK, Shukla Y. Herbal medicine: current status and the future. Asian Pacific Journal of Cancer Prevention. 2003;4(4):281-8.
5. Choudhary GP, Jain AP. Evaluation of Acute, Subacute and LD 50 values of Methanolic extract of Sphaeranthus indicus leaves in Albino mice. Research Journal of Pharmacy and Technology. 2021;14(5):2487-92.DOI : 10.52711/0974-360X.2021.00438
6. Chen X, Zhou H, Liu YB, Wang JF, Li H, Ung CY, Han LY, Cao ZW, Chen YZ. Database of traditional Chinese medicine and its application to studies of mechanism and to prescription validation. British journal of pharmacology. 2006;149(8):1092-103. https://doi.org/10.1038/sj.bjp.0706945
7. Koduru S, Grierson DS, Afolayan AJ. Antimicrobial Activity of Solanum aculeastrum. Pharmaceutical biology. 2006;44(4):283-6.doi:https://doi.org/10.1080/13880200600714145
8. Purnomo Y, Aini N, Noerhayati E. Acute Toxicity Level of Pulutan (Urena lobata) Leaf Extract on Zebrafish (Danio rerio) and its Analysis by In Silico Study. Research Journal of Pharmacy and Technology. 2022;15(6):2477-82. DOI: 10.52711/0974-360X.2022.00413
9. Hunter P. A toxic brew we cannot live without: micronutrients give insights into the interplay between geochemistry and evolutionary biology. EMBO reports. 2008; 9(1):15-8.Doi: https://doi.org/10.1038/sj.embor.7401148
10. Yadav T, Rohane S. Acute toxicity study of synthesized drug and herbal product. Asian Journal of Pharmaceutical Research. 2021; 11(4): 251-6.DOI: 10.52711/2231-5691.2021.00044
11. Michalak M, Gustaw K, Waśko A, Polak-Berecka M. Composition of lactic acid bacteria during spontaneous curly kale (Brassica oleracea var. sabellica) fermentation. Microbiological Research. 2018;206:121-30.doi: https://doi.org/10.1016/j.micres.2017.09.011
12. Khalid W, Sajid Arshad M, Imran M, Shabir Ahmad R, Imran A, Qaisrani TB, Asghar Z, Husain A, Muhammad Anjum F, Ansar Rasul Suleria H. Kale (Brassica oleracea var. sabellica) as miracle food with special reference to therapeutic and nutraceuticals perspective. Food Science & Nutrition. 2021. doi: https://doi.org/10.1002/fsn3.2476
13. Casajús V, Perini M, Ramos R, Lourenco AB, Salinas C, Sánchez E, Fanello D, Civello P, Frezza D, Martínez G. Harvesting at the end of the day extends postharvest life of kale (Brassica oleracea var. sabellica). Scientia Horticulturae. 2021;276:109757.doi: https://doi.org/10.1016/j.scienta.2020.109757
14. Schmidt S, Zietz M, Schreiner M, Rohn S, Kroh LW, Krumbein A. Genotypic and climatic influences on the concentration and composition of flavonoids in kale (Brassica oleracea var. sabellica). Food Chemistry. 2010;119(4):1293-9.doi: https://doi.org/10.1016/j.foodchem.2009.09.004
15. Doss A. Preliminary phytochemical screening of some Indian medicinal plants. Ancient science of life. 2009;29(2):12.doi: https://doi.org/10.1016/j.foodchem.2009.09.004.
16. Khandelwal K. Practical pharmacognosy. Pragati Books Pvt. Ltd.; 2008 Sep 7.
17. Burakale PV, Bhise MR, Sakarkar DM, Sudke SG. Synthesis and assessment of sub-acute toxicity of novel rosin esters of polyethylene glycol 200 in swiss albino mice. Research Journal of Pharmacy and Technology. 2021;14(4):1859-66.DOI: 10.52711/0974-360X.2021.00329
18. Dubey SK, Batra A. Acute and sub acute toxicity studies on ethanolic fraction of Thuja occidentalis Linn. Research Journal of Pharmacy and Technology. 2008;1(3):245-8.
19. Shrivastava A, Jain R, Agrawal RK, Ahirwar D. Clinical trial of herbal drugs and products in India: Past and current status and critical issues. Research Journal of Pharmacy and Technology. 2008;1(2):69-74.
20. Ahvazi M, Khalighi-Sigaroodi F, Charkhchiyan MM, Mojab F, Mozaffarian VA, Zakeri H. Introduction of medicinal plants species with the most traditional usage in Alamut region. Iranian journal of pharmaceutical research: IJPR. 2012;11(1):185.
21. Setzer RW, Kimmel CA. Use of NOAEL, benchmark dose, and other models for human risk assessment of hormonally active substances. Pure and Applied Chemistry. 2003;75(11-12):2151-8.https://doi.org/10.1351/pac200375112151
22. Ouidad A, Sara C, Samir D. Biological properties and Acute Toxicity Study of Copper oxide nanoparticles prepared by aqueous leaves extract of Portulaca oleracea (L). Asian Journal of Pharmaceutical Research. 2020;10(2):89-94.1 DOI : 10.5958/2231-5691.2020.00017.9
23. Vijayalakshmi S, Kripa KG, Suganthi V. Toxicological study of hydroalcoholic extract of Blepharismaderaspatensis (L.) heyne ex roth in wistar albino rats. Research Journal of Pharmacy and Technology. 2020;13(12):5673-9.DOI : 10.5958/0974-360X.2020.00988.9
24. Saleem U, Amin S, Ahmad B, Azeem H, Anwar F, Mary S. Acute oral toxicity evaluation of aqueous ethanolic extract of Saccharum munjaRoxb. roots in albino mice as per OECD 425 TG. Toxicology Reports. 2017;4:580-5. https://doi.org/10.1016/j.toxrep.2017.10.005
25. Saleem U, Ahmad B, Ahmad M, Erum A, Hussain K, Irfan Bukhari N. Is folklore use of Euphorbia helioscopia devoid of toxic effects?. Drug and Chemical Toxicology. 2016;39(2):233-7. https://doi.org/10.3109/01480545.2015.1092040
26. Morita Y, Ueno T, Sasaki N, Kuhara K, Yoshioka S, Tateishi Y, Nagata E, Kage M, Sata M. Comparison of Liver Histology Between Patients with Non‐Alcoholic Steatohepatitis and Patients with Alcoholic Steatohepatitis in Japan. Alcoholism: Clinical and Experimental Research. 2005;29:277S-81S.https://doi.org/10.1097/01.alc.0000191777.36629.33
27. Lackner C, Gogg-Kamerer M, Zatloukal K, Stumptner C, Brunt EM, Denk H. Ballooned hepatocytes in steatohepatitis: the value of keratin immunohistochemistry for diagnosis. Journal of hepatology. 2008;48(5):821-8.https://doi.org/10.1016/j.jhep.2008.01.026
28. Jain N, Sharma P, Sharma N, Joshi SC. Haemato-biochemical profile following sub-acute toxicity of malathion male albino rats. Avicenna J. 2009.