Author(s): Kedar Prasad Meena, Purandhar Choudhary, Tripty Karri, Pradeep Samal


DOI: 10.52711/0974-360X.2023.00789   

Address: Kedar Prasad Meena*, Purandhar Choudhary, Tripty Karri, Pradeep Samal
Department of Pharmacy, Guru Ghasidas Vishwavidyalaya, Bilaspur, Chhattisgarh, India.
*Corresponding Author

Published In:   Volume - 16,      Issue - 10,     Year - 2023

Rutin is a natural molecule proved for strong efficacy against type 2 diabetes. Clinical application of rutin is highly restricted because of its low bioavailability and ineffectiveness in in vivo conditions. Therefore, the main aim of the present investigation was to formulate rutin loaded microspheres and evaluated for its efficacy against diabetes. Rutin loaded microparticles formulation was successfully prepared by emulsion solvent evaporation technique, characterized and evaluated for its potential application in diabetes. The rutin microparticles showing highest entrapment efficiency of 82.2±0.04% and higher percentage yield of 68.46±1.33% was selected as optimized formulation. Particle size and zetapotential of optimized Rutin microparticles formulation was found to be 210.78±5.46 µm and -0.0354mV, respectively. TEM and SEM images showed spherical shape and smooth surface of microparticle formulation. Rutin microparticles showed sustained drug release up to 6 h without any burst release. FTIR results revealed absence of potential chemical interaction between Rutin and other excipients of microparticles. DSC and XRD studies revealed the conversion of Rutin from crystalline to amorphous form. Stability studies indicate that the Rutin microspheres were stable and the formulation characteristics remain unchanged up to 30 days in room temperature and elevated temperature. Rutin microparticles treated rats showed significantly lower blood glucose, cholesterol, LDL, free fatty acid and triglyceride concentrations in comparison to pristine Rutin treated rats. The glucose and lipid profiles of microsphere formulation were akin to normal rats. Moreover, Rutin microparticles did not produce obesity (common adverse effect of antidiabetic drugs) even after 60 days. These results evidently indicate the potential and safe utilization of Rutin microparticle formulation for the treatment of diabetes.

Cite this article:
Kedar Prasad Meena, Purandhar Choudhary, Tripty Karri, Pradeep Samal. Preparation and Characterization of Rutin Loaded Microparticles for the treatment of Diabetes. Research Journal of Pharmacy and Technology 2023; 16(10):4867-4. doi: 10.52711/0974-360X.2023.00789

Kedar Prasad Meena, Purandhar Choudhary, Tripty Karri, Pradeep Samal. Preparation and Characterization of Rutin Loaded Microparticles for the treatment of Diabetes. Research Journal of Pharmacy and Technology 2023; 16(10):4867-4. doi: 10.52711/0974-360X.2023.00789   Available on:

1.    American Diabetes, A. Diagnosis and Classification of Diabetes Mellitus. Diabetes Care. 2009; 32: S62-S67.
2.    Auclair, S., Milenkovic, D., Besson, C., Chauvet, S., Gueux, E., Morand, C., Mazur, A. and Scalbert, A. Catechin reduces atherosclerotic lesion development in apo E-deficient mice: a transcriptomic study. Atherosclerosis. 2009; 204: e21-7.
3.    Bhardwaj, P., Khanna, D. and Balakumar, P. Catechin averts experimental diabetes mellitus-induced vascular endothelial structural and functional abnormalities. Cardiovasc Toxicol. 2014; 14: 41-51.
4.    Dugas, A.J., Jr., Castaneda-Acosta, J., Bonin, G.C., Price, K.L., Fischer, N.H. and Winston, G.W. Evaluation of the total peroxyl radical-scavenging capacity of flavonoids: structure-activity relationships. J Nat Prod. 2000; 63, 327-31.
5.    Fukuhara, K., Nakanishi, I., Kansui, H., Sugiyama, E., Kimura, M., Shimada, T., Urano, S., Yamaguchi, K. and Miyata, N. Enhanced Radical-Scavenging Activity of a Planar Catechin Analogue. Journal of the American Chemical Society. 2002; 124, 5952-5953.
6.    Mandel, S. and Youdim, M.B. Catechin polyphenols: neurodegeneration and neuroprotection in neurodegenerative diseases. Free Radic Biol Med. 2004; 37: 304-17.
7.    Mandel, S.A., Avramovich-Tirosh, Y., Reznichenko, L., Zheng, H., Weinreb, O., Amit, T. and Youdim, M.B. Multifunctional activities of green tea catechins in neuroprotection. Modulation of cell survival genes, iron-dependent oxidative stress and PKC signaling pathway. Neurosignals. 2005; 14: 46-60.
8.    Meeran, S.M., Mantena, S.K., Elmets, C.A. and Katiyar, S.K.  (-)-Epigallocatechin-3-gallate prevents photocarcinogenesis in mice through interleukin-12-dependent DNA repair. Cancer Res. 2006; 66: 5512-20.
9.    Miura, Y., Chiba, T., Tomita, I., Koizumi, H., Miura, S., Umegaki, K., Hara, Y., Ikeda, M. and Tomita, T. Tea catechins prevent the development of atherosclerosis in apoprotein E-deficient mice. J Nutr. 2001; 131: 27-32.
10.    Miyata, Y., Tamaru, S., Tanaka, T., Tamaya, K., Matsui, T., Nagata, Y. and Tanaka, K. Theflavins and theasinensin A derived from fermented tea have antihyperglycemic and hypotriacylglycerolemic effects in KK-A(y) mice and Sprague-Dawley rats. J Agric Food Chem. 2013; 61: 9366-72.
11.    Rice-Evans, C.A., Miller, N.J. and Paganga, G. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radic Biol Med. 1996; 20: 933-56.
12.    Singh, B., Vuddanda, P.R., M, R.V., Kumar, V., Saxena, P.S. and Singh, S. Cefuroxime axetil loaded solid lipid nanoparticles for enhanced activity against S. aureus biofilm. Colloids Surf B Biointerfaces. 2014; 121: 92-8.
13.    Tang, F.Y., Nguyen, N. and Meydani, M.  Green tea catechins inhibit VEGF-induced angiogenesis in vitro through suppression of VE-cadherin phosphorylation and inactivation of Akt molecule. Int J Cancer. 2003; 106: 871-8.
14.    Vijayakumar, M.R., Kosuru, R., Singh, S.K., Prasad, C.B., Narayan, G., Muthu, M.S. and Singh, S. Resveratrol loaded PLGA:d-[small alpha]-tocopheryl polyethylene glycol 1000 succinate blend nanoparticles for brain cancer therapy. RSC Advances. 2016; 6: 74254-74268.
15.    Vijayakumar, M.R., Kosuru, R., Vuddanda, P.R., Singh, S.K. and Singh, S. Trans resveratrol loaded DSPE PEG 2000 coated liposomes: Evidence for prolonged systemic circulation and passive brain targeting. Journal of Drug Delivery Science and Technology. 2016; 33: 125-135.
16.    Vijayakumar, M.R., Kumari, L., Patel, K.K., Vuddanda, P.R., Vajanthri, K.Y., Mahto, S.K. and Singh, S. Intravenous administration of trans resveratrol loaded TPGS coated solid lipid nanoparticles for prolonged systemic circulation, passive brain targeting and improved in vitro cytotoxicity against C6 glioma cell lines. RSC Advances. 2016.
17.    Vijayakumar, M.R., Vajanthri, K.Y., Balavigneswaran, C.K., Mahto, S.K., Mishra, N., Muthu, M.S. and Singh, S. Pharmacokinetics, biodistribution, in vitro cytotoxicity and biocompatibility of Vitamin E TPGS coated trans resveratrol liposomes. Colloids and Surfaces B: Biointerfaces. 145: 479-491.
18.    Wang, L.F. and Zhang, H.Y., 2005. A theoretical study of the different radical-scavenging activities of catechin, quercetin, and a rationally designed planar catechin. Bioorg Chem. 2016; 33: 108-15.
19.    Zhu, D., Wang, L., Zhou, Q., Yan, S., Li, Z., Sheng, J. and Zhang, W. (+)-Catechin ameliorates diabetic nephropathy by trapping methylglyoxal in type 2 diabetic mice. Mol Nutr Food Res. 2014; 58: 2249-60.

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

56th percentile
Powered by  Scopus

SCImago Journal & Country Rank

Recent Articles


Not Available