Author(s):
Uttam Prasad Panigrahy, Siva Prasad Panda, Biplab Kumar Dey
Email(s):
uttampanigrahy@gmail.com
DOI:
10.52711/0974-360X.2023.00787 
Address:
Uttam Prasad Panigrahy1*, Siva Prasad Panda2, Biplab Kumar Dey3
1,3Faculty of Pharmaceutical Sciences, Assam Down Town University,
Sankar Madhab Path, Gandhi Nagar, Panikhaiti, Guwahati, Assam, India.
2Institute of Pharmaceutical Research, GLA University, Mathura, UP, India.
*Corresponding Author
Published In:
Volume - 16,
Issue - 10,
Year - 2023
ABSTRACT:
Objective: A simultaneous estimation of Esomeprazole and Ondansetron by HPLC-DAD method with degradation studies was developed. Method: It was carried out using Kromasil (150mm×4.6mm, 5µm particle size) column with mobile phase consists 0.01M KH2PO4 Phosphate buffer (1mL of Triethylamine added and pH was adjusted to 3.5 with Orthophosphoric acid): Acetonitrile (35:65, %v/v) with flow rate 1mL/min at 265nm. Esomeprazole and Ondansetron were eluted at 2.350 and 2.831mins respectively. Results and Discussion: Validation was performed as per ICH guidelines, which shows linearity 25-150µg/mL of Esomeprazole and 10-60µg/mL of Ondansetron with R2 was 0.999. Accuracy was obtained to be 99.75-100.21% for Esomeprazole and 99.39-100.3% for Ondansetron respectively and precision which shows %RSD less than 2%. Stress studies were carried out which shows that both Esomeprazole and Ondansetron is moderately degraded in acid, base, peroxide and thermal degradation and less degraded in photolytic degradation. Conclusion: This method was applicable for routine analysis and stability of Esomeprazole and Ondansetron.
Cite this article:
Uttam Prasad Panigrahy, Siva Prasad Panda, Biplab Kumar Dey. A Novel Analytical approach for Simultaneous Estimation of Esomeprazole and Ondansetron by HPLC-DAD Method with Degradation Studies. Research Journal of Pharmacy and Technology 2023; 16(10):4855-0. doi: 10.52711/0974-360X.2023.00787
Cite(Electronic):
Uttam Prasad Panigrahy, Siva Prasad Panda, Biplab Kumar Dey. A Novel Analytical approach for Simultaneous Estimation of Esomeprazole and Ondansetron by HPLC-DAD Method with Degradation Studies. Research Journal of Pharmacy and Technology 2023; 16(10):4855-0. doi: 10.52711/0974-360X.2023.00787 Available on: https://rjptonline.org/AbstractView.aspx?PID=2023-16-10-57
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