Author(s): Sreejesh Pilakkavil Chirakkara, Jiji George, Asha Abraham


DOI: 10.52711/0974-360X.2023.00786   

Address: Sreejesh Pilakkavil Chirakkara1*, Jiji George2, Asha Abraham2
1Department of Biotechnology, St Aloysius College (Autonomous), Mangalore, Karnataka, India.
2Department of Postgraduate Studies and Research in Biotechnology, St Aloysius College (Autonomous), Mangalore, Karnataka, India.
*Corresponding Author

Published In:   Volume - 16,      Issue - 10,     Year - 2023

The impact of bacterial microbiome metabolites on human health, most particularly on the prevention and treatment of cancer, has gained significant interest in recent times. The study focused on the isolation of bacterial flora associated with the ovaries of 8-week-old mice and examined its ability to produce metabolites with potential antibacterial, antioxidant, and anticancer properties. The isolates were identified based on biochemical and 16S rRNA sequencing. The ethyl acetate extract from isolates was screened for antimicrobial and antioxidant capacity. The 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay was used to evaluate the cytotoxicity of the selected isolate against the ovarian carcinoma cell lines SKOV-3 and PA-1. The ethyl acetate extract from Bacillus velezensis OM03 exhibited significant antibacterial activity against all the tested bacteria, with a MIC value ranging from 50 to 100 µg/mL. Furthermore, the extracts demonstrated hydrogen peroxide and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activities at rates of 88.50% and 87.78%, respectively. The extracts displayed substantial concentration-dependent antiproliferative/cytotoxic activities against SKOV-3 and PA-1 cell lines after 24 and 48 h of treatments, respectively. Further chemical analysis of the extract using HR-LCMS revealed the presence of bioactive compounds such as myriocin, 2,3-diethylpyrazine, dihydrodeoxystreptomycin, cyclo (L-Phe-L-Pro), C16 sphinganine, and other twenty-nine compounds that have been formerly reported and are accountable for the targeted activities. Bacillus velezensis OM03 may be further investigated for the creation of novel therapeutics, particularly for the treatment of ovarian cancer.

Cite this article:
Sreejesh Pilakkavil Chirakkara, Jiji George, Asha Abraham. Mice ovarian microbiome investigation divulges prospective fount of anticancer and antimicrobial metabolites. Research Journal of Pharmacy and Technology 2023; 16(10):4847-4. doi: 10.52711/0974-360X.2023.00786

Sreejesh Pilakkavil Chirakkara, Jiji George, Asha Abraham. Mice ovarian microbiome investigation divulges prospective fount of anticancer and antimicrobial metabolites. Research Journal of Pharmacy and Technology 2023; 16(10):4847-4. doi: 10.52711/0974-360X.2023.00786   Available on:

1.    Yu ZK.Xie RL.You al. The role of the bacterial microbiome in the treatment of cancer. BMC Cancer. 2021; 21(1): 1-14. doi:10.1186/s12885-021-08664-0
2.    Nguyen TLA.Vieira-Silva S.Liston A.Raes J. How informative is the mouse for human gut microbiota research? DMM disease Models and Mechanisms. 2015; 8(1): 1-16. doi:10.1242/DMM.017400
3.    Nair N.Kasai T.Seno M. Bacteria: Prospective savior in battle against cancer. Anticancer Research. 2014;34(11):6289-6296.
4.    Miles SM.Hardy BL.Merrell DS. Investigation of the microbiota of the reproductive tract in women undergoing a total hysterectomy and bilateral salpingo-oopherectomy. Fertility and Sterility. 2017; 107(3): 813-820. e1. doi:10.1016/j.fertnstert.2016.11.028
5.    Zhou B.Sun C.Huang al. The biodiversity composition of microbiome in ovarian carcinoma patients. Scientific Reports. 2019; 9(1): 1-11. Accessed November 24, 2022. /pmc/articles/PMC6368644/
6.    Mohanta M.Khanam S.Islam MS.Mohanta MK. Isolation, characterization and identification of bacterial isolates from the poultry environment at Rajshahi Metropolis, Bangladesh. Journal of Entomology and Zoology Studies. 2017; 5(4):918-926.
7.    Sipos A.Ujlaki G.Mikó al. The role of the microbiome in ovarian cancer: mechanistic insights into oncobiosis and to bacterial metabolite signaling. Molecular Medicine. 2021;27(1). doi:10.1186/s10020-021-00295-2
8.    Deore ND.Gupta S.Shrivastav B.Upasni CD.Apte KG.Shaikh AM. Evidence based evaluation of antidiabetic potential of Yesaka on Streptozotocin diabetic rats. Research Journal of Pharmacy and Technology. 2018;11(11):4965-4970. doi:10.5958/0974-360X.2018.00904.6
9.    Masato Ohtsuka MS.Akayuki Sakurai SN.Yukiko Yasuoka SW. Functional recovery of a whole ovary transplanted into syngenic testis in mice. Cloning and Transgenesis. 2012;01(01):1-4. doi:10.4172/2168-9849.1000102
10.    Al-Shimmary SMH.Mohamed NS.Al-Qaysi SAS.Almohaidi AMS. Phylogeny analysis of gyrB Gene and 16S rRNA genes of Pseudomonas aeruginosa Isolated from Iraqi Patients. Research Journal of Pharmacy and Technology. 2021;14(5):2517-2521. doi:10.52711/0974-360X.2021.00443
11.    Kumar S.Stecher G.Li M.Knyaz C.Tamura K. MEGA X: Molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution. 2018;35(6):1547-1549. doi:10.1093/molbev/msy096
12.    Ravikumar S.Fredimoses M.Gnanadesigan M. Anticancer property of sediment actinomycetes against MCF-7 and MDA-MB-231 cell lines. Asian Pacific Journal of Tropical Biomedicine. 2012; 2(2):92-96. doi:10.1016/S2221-1691(11)60199-8.
13.    Vaijayanthi G.Cholarajan A.Vijayakumar R. Isolation, characterization and antibacterial activity of terrestrial actinobacteria in the soils of Thanjavur District, Tamil Nadu, India. Research Journal of Science and Technology. 2013;4(3):132-139.
14.    Vasavada H.Inampudi S. Minimal inhibitory concentration and minimal bactericidal concentration of Peppermint (Mentha piperita) extracts against standard microorganisms. Research Journal of Pharmacy and Technology. 2021; 14(9):4662-4666. doi:10.52711/0974-360X.2021.00810
15.    Chitra V. Tamilanban T. Manasa K. Narayanan J. Evaluation of in-vitro antioxidant activity of ethanolic extract of ugli fruit. Research Journal of Pharmacy and Technology. 2017;10(11):3826-3830. doi:10.5958/0974-360X.2017.00694.1
16.    Karthik VP.Punnagai.Suresh P.David DC. In vitro hydrogen peroxide scavenging activity and alpha amylase inhibitory activity of Croton tiglium extract. Research Journal of Pharmacy and Technology. 2019; 12(6): 3045-3047. doi:10.5958/0974-360X.2019.00515.8
17.    Sasikala M.Sundaraganapathy R.Mohan S. MTT assay on anticancer properties of phytoconstituents from Ipomoea aquatica forsskal using MCF–7 cell lines for breast cancer in women. Research Journal of Pharmacy and Technology. 2020; 13(3):1356-1360. doi:10.5958/0974-360X.2020.00250.4
18.    Chethankumara GP.Krishna V.Nagaraj K. HR-LCMS and In vitro cytotoxicity analysis of Alseodaphne semecarpifolia stem bark and leaf methanol extracts. Research Journal of Pharmacy and Technology. 2022; 15(1): 250-256. doi:10.52711/0974-360X.2022.00041
19.    Meidong R.Khotchanalekha K.Doolgindachbaporn al. Evaluation of probiotic Bacillus aerius B81e isolated from healthy hybrid catfish on growth, disease resistance and innate immunity of Pla-mong Pangasius bocourti. Fish and Shellfish Immunology. 2018;73:1-10. doi:10.1016/j.fsi.2017.11.032
20.    Setyawati T.Yanti A.Kurniatuhadi R. Identification and Probiotic potency screening of cellulolytic bacilli isolated from fecal pellets and gastrointestinal tract of Nypha worm (Namalycastis rhodochorde), West Kalimantan, Indonesia. Jurnal Biologi Indonesia. 2021;17(2):189-195. doi:10.47349/jbi/17022021/189
21.    Zhang L.Yi H. Potential antitumor and anti-inflammatory activities of an extracellular polymeric substance (EPS) from Bacillus subtilis isolated from a housefly. Scientific Reports. 2022; 12(1):1-10. doi:10.1038/s41598-022-05143-9
22.    Wang J. Zhang D. Wang Y.Liu Z.Liu L. Shi C. Probiotic effects of the Bacillus velezensis GY65 strain in the mandarin fish, Siniperca chuatsi. Aquaculture Reports. 2021;21:100902. doi:10.1016/j.aqrep.2021.100902
23.    Rabbee MF.Ali S.Baek K hyun. Endophyte Bacillus velezensis isolated from Citrus. controls Streptomycin-resistant Xanthomonas citri subsp. citri that causes citrus bacterial canker. Agronomy. 2019; 9(8): 470.
24.    Maheswari P.Mahendran S.Sankaralingam S.Sivakumar N.  In vitro antioxidant activity of exopolysaccharide extracted from marine sediment soil bacteria . Research Journal of Pharmacy and Technology. 2020; 13(1): 404. doi:10.5958/0974-360x.2020.00079.7
25.    Chirakkara SP.Abraham A. Bacillus strains from the ovaries of Swiss albino mice (Mus musculus): deciphering of probiotic potential through an in vitro approach. Biomedicine. 2022; 42(6): 1200-1208. doi:10.51248/.V42I6.2302
26.    Pournejati R.Karbalaei-Heidari HR. Optimization of fermentation conditions to enhance cytotoxic metabolites production by Bacillus velezensis strain RP137 from the Persian gulf. Avicenna Journal of Medical Biotechnology. 2020; 12(2):116-123.

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

56th percentile
Powered by  Scopus

SCImago Journal & Country Rank

Recent Articles


Not Available