Author(s): Janani Manoharan, Nirmal Kumar Chinnakannu, Babujanarthanam Ranganathan


DOI: 10.52711/0974-360X.2023.00783   

Address: Janani Manoharan1, Nirmal Kumar Chinnakannu2, Babujanarthanam Ranganathan3*
1Department of Biochemistry, Auxilium College (Autonomous), Gandhi Nagar, Vellore - 632006, Tamil Nadu, India.
2Department of Biochemistry, KMG College of Arts and Science, Gudiyattam, Vellore - 632602, Tamil Nadu, India.
3Nano and Energy Bioscience Laboratory, Department of Biotechnology, Thiruvalluvar University, Serkkadu, Vellore - 632115, Tamil Nadu, India.
*Corresponding Author

Published In:   Volume - 16,      Issue - 10,     Year - 2023

Herbal medicaments are applied in the healing for numerous human illnesses from the sprouting of human civilization. The chemicals existed naturally within the plants displays antiradical features which are the principal agent for the neutralization of reactive oxygen species created within the living entities. Acacia nilotica from the Fabaceae family is a tree with magnificent therapeutic properties. Various sections of this tree are implemented in the traditional healing system. Specifically, the bark are applied to treat numerous health complaints. Here, owned to the medicinal features of bark of A.nilotica in the traditional practices, we prepared the aqueous bark extract from this tree and evaluated the antioxidant (antiradical) and anti-inflammatory activities. Hydroxyl radical scavenging protocol presented dose-based upsurge in the radical inhibition activity of the bark extract with maximum activity at 60 µg/ml and the IC50 value appeared as 43.35µg/ml. ABTS radical scavenging assay executed also displayed dose-lineated upsurge in the radical scavenging property of the tested bark extract with IC50 value as 41.07µg/ml. In both the radical scavenging assays, vitamin C was implemented for reference purpose. Even, the anti-inflammatory nature of the aqueous bark extract was performed applying egg albumin denaturation inhibition protocol. In a dose-lined way, the bark extract presented anti-inflammatory activity with an IC50 calculated value as 73.62µg/ml with diclofenac sodium as reference medicament. Additionally, the phytochemical qualitative scrutiny presented the existence of steroids, saponins, terpenoids, alkaloids, tannins, anthraquinones, carbohydrates, phenols and flavonoids which were chiefly responsible for the pharmacological activities of bark aqueous extract.

Cite this article:
Janani Manoharan, Nirmal Kumar Chinnakannu, Babujanarthanam Ranganathan. Phytochemical screening, Antiradical and Anti-inflammatory activity of aqueous bark extract acquired from Acacia nilotica. Research Journal of Pharmacy and Technology 2023; 16(10):4831-5. doi: 10.52711/0974-360X.2023.00783

Janani Manoharan, Nirmal Kumar Chinnakannu, Babujanarthanam Ranganathan. Phytochemical screening, Antiradical and Anti-inflammatory activity of aqueous bark extract acquired from Acacia nilotica. Research Journal of Pharmacy and Technology 2023; 16(10):4831-5. doi: 10.52711/0974-360X.2023.00783   Available on:

1.    Varsha Singh, Aleza Rizvi, Udaivir Singh Sara. Standardization and Phytochemcial screening of Carica papaya seeds. Research Journal of Pharmacy and Technology. 2021; 14(9):4540-6. doi: 10.52711/0974-360X.2021.00790
2.    Sumathi R, Pavni S, Sivakumar T. Antimicrobial evaluation of lipid extract of Pongamiapinnataleaveas. Research Journal of Pharmacy and Technology. 2009; 2(4):714-718.
3.    VeenaKadasala, NiladrySekhar Ghosh, Santhosh Kumar Chinnaiyan, Arunabha Mallik, Manjunath SY, Chiranjib Bhattacharjee. Phytochemical and Pharmacological Evaluation of Annona reticlulate. Research Journal of Pharmacy and Technology. 2021; 14(9):4881-4886. Doi:10.52711/0974-360X.2021.00848.
4.    Rekha Rajendran, R Hemachander, T Ezhilarasan, C Keerthana, DL Saroja, KV Saichand, Mohamed Gasim Abdullah. Phytochemical Analysis and In-vitro Antioxidant activity of Mimosa pudica Lin, Leaves. Research Journal of Pharmacy and Technology. 2010; 3(2): 551-555.
5.    Joshi Uttara, Upadhye Mohini. Evaluation of Antioxidant activity of Aqueous extract bark of Ficus glomerata. Research Journal of Pharmacy and Technology. 2008; 1 (4):537-538.
6.    Chew KheJiea, ShivkanyaFuloria, VetriselvanSubrimanyan, MahendranSekar, KathiresanV.Sathasivam, SaminathanKayarohanam, Yuan Seng Wu, VenkataSathyaSaiappala Raju Velago, Ashok Kumar Janakiraman, Mohammad Nazmul Hasan Maziz, Neeraj Kumar Fuloria. Phytochemcial screening and Antioxidant activity of Canaga odorata extract. Research Journal of Pharmacy and Technology. 2022; 15(3):1230-1234. doi: 10.52711/0974-360X.2022.00205.
7.    Chandra S, Chatterjee P, Dey P, Bhattacharya S. Evaluation of in vitro anti-inflammatory activity of coffee against the denaturation of protein. Asian Pacific Journal of Tropical Biomedicine. 2012;2(1 SUPPL.):S178–80. Available from:
8.    Barton GM. A calculated response: Control of inflammation by the innate immune system. Journal of Clinical Investigation. 2008;118(2):413–420.
9.    Ren K, Dusad A, Dong R, Quan L. Albumin as a delivery carrier for rheumatoid arthritis. J Journal of Nanomedicine and Nanotechnology. 2013;4(4):176. doi: 10.4172/2157-7439.1000176
10.    Arya D, Meena M, Neha G, Vidya P. In vitro anti-inflammatory and anti-arthritic activity in methanolic extract of Cocculushirsutus (L.) Diels. In vivo and In vitro. International Journal of Pharmaceutical Sciences and Research2014;5(5):1957-1962.
11.    Thirumalaisamy R, Ammashi S, Muthusamy G. Screening of anti-inflammatory phytocompounds from Cratevaadansonii leaf extracts and its validation by in silicomodeling. Journal of Genetic Engineering and Biotechnology. 2018;16(2):711–719. Available from:
12.    Furman D, Campisi J, Verdin E, Carrera-Bastos P, Targ S, Franceschi C, et al. Chronic inflammation in the etiology of disease across the life span. Nature Medicine. 2019;25(12):1822–1832. Available from:
13.    Fokunang C. Overview of non-steroidal anti-inflammatory drugs (nsaids) in resource limited countries. MOJ Toxicology. 2018;4(1):5–13. doi: 10.15406/mojt.2018.04.00081
14.    Lagana P, Anastasi G, Marano F, Piccione S, Singla RK, Dubey AK, et al. Phenolic substances in foods: Health effects as anti-inflammatory and antimicrobial agents. Journal of AOAC International. 2019;102(5):1378–1387.
15.    Baravkar AA, Kale RN, Patil RN, Sawant SD. Pharmaceutical and Biological evaluation of formulated cream of methanolic extract of Acacia niloticaleaves. Research Journal of Pharmacy and Technology. 2008; 1(4):480-483.
16.    Atif Ali, Naveed Akhtar, Barkat Ali Khan, Muhammad Shoaib Khan, Akhtar Rasul,Shahiq-UZ-Zaman, Nayab Khalid, Khalid Waseem, Tariq Mahmood and Laiquat Ali. Acacia nilotica: A plant of multipurpose medicinal uses. Journal of Medicinal Plants Research. 2012;6(9):1492–1496. doi: 10.5897/JMPR11.1275
17.    Iqbal E, Salim KA, Lim LBL. Phytochemical screening, total phenolics and antioxidant activities of bark and leaf extracts of Goniothalamusvelutinus (Airy Shaw) from Brunei Darussalam. Journal of King Saud University - Science. 2015;27(3):224–232. Available from:
18.    Subramanian R, Subbramaniyan P, Raj V. Antioxidant activity of the stem bark of Shorearoxburghii and its silver reducing power. Springerplus. 2013; 2(1):1–11. doi:
19.    Kuppurangan G, Karuppasamy B, Nagarajan K, KrishnasamySekar R, Viswaprakash N, Ramasamy T. Biogenic synthesis and spectroscopic characterization of silver nanoparticles using leaf extract of Indoneesiellaechioides: in vitro assessment on antioxidant, antimicrobial and cytotoxicity potential. Applied Nanoscience 2016;6(7):973–982. doi:
20.    Karthik K, P BRK, R VP, K SK, Rathore RSB. Evaluation of Anti-Inflammatory Activity of CanthiumParviflorum By in-Vitro Method. Indian Journal of Research in Pharmacy and Biotechnology. 2013;5674(October):729-730.
21.    Treml J, Smejkal K. Flavonoids as Potent Scavengers of Hydroxyl Radicals. Comprehensive Reviews in Food Science and Food Safety. 2016;15(4):720–738. doi:
22.    Ozyurek M, Bektasoglu B, Güçlü K, Apak R. Hydroxyl radical scavenging assay of phenolics and flavonoids with a modified cupric reducing antioxidant capacity (CUPRAC) method using catalase for hydrogen peroxide degradation. AnalyticaChimicaActa. 2008;616(2):196–206.doi: 10.1016/j.aca.2008.04.033
23.    Leong LP, Shui G. An investigation of antioxidant capacity of fruits in Singapore markets. Food Chemistry. 2002;76(1):69–75.doi:

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

56th percentile
Powered by  Scopus

SCImago Journal & Country Rank

Recent Articles


Not Available