Author(s): Citra Dewi Maharani, Nurwasis, Delfitri Lutfi, Clarisa Finanda, Kautsar Abiyoga, Evelyn Komaratih, Yulia Primitasari, Wimbo Sasono, Djoko Legowo


DOI: 10.52711/0974-360X.2023.00772   

Address: Citra Dewi Maharani1, Nurwasis1*, Delfitri Lutfi1, Clarisa Finanda1, Kautsar Abiyoga1, Evelyn Komaratih1, Yulia Primitasari1, Wimbo Sasono1, Djoko Legowo2
1Department of Ophthalmology, Faculty of Medicine Universitas Airlangga – Dr. Soetomo General Academic Hospital, Surabaya, Indonesia.
2Department of Pathology Anatomy, Faculty of Veterinary Universitas Airlangga – Animal Hospital, Universitas Airlangga Academic Hospital, Surabaya, Indonesia.
*Corresponding Author

Published In:   Volume - 16,      Issue - 10,     Year - 2023

Aims: To assess the effects of intravitreal triamcinolone acetonide and bevacizumab on the expression of matrix metalloproteinase MMP-2 and its inhibitor TIMP-1 in an experimental rabbit model of penetrating injury. Settings and Design: An accurate experimental study of five left eyes as negative control and 27 eyes with penetrating injury with or without treatment from 27 rabbits. Methods and Material: A total of 30 New Zealand rabbits were recruited, and penetrating injury was performed in the superotemporal quadrant of the right eye by making incisions 5 mm horizontally and 6 mm behind the limbus. The rabbits were split into five groups: OGI, intravitreal triamcinolone acetonide, and bevacizumab, with varying injection timings (n = 6 per group). All eyes were inspected and analyzed by assessing the expression of MMP-2 and TIMP-1. Statistical analysis used: Statistical analysis was performed using the Prism GraphPad 9. Statistical calculations were made by ANOVA test. All descriptive data are presented as mean+standard deviation. P value less than 0.05 was considered significant statistically. Results: The expression of MMP-2 in the treatment group was considerably lower than in control penetrating injury group (8,36±1,699, p<0,0001), conversely TIMP-1 expression was higher in the treatment group (4,72±1,026, P 0,0593). Fibrosis was assessed with HE staining and primarily detected in positive control groups. Conclusions: TA and bevacizumab treatments after penetrating injury effectively inhibited the elevation of MMP-2 and decreased the expression of TIMP-1 in the retina and wound site tissue, respectively. It reduces the possibility of acquiring posttraumatic PVR.

Cite this article:
Citra Dewi Maharani, Nurwasis, Delfitri Lutfi, Clarisa Finanda, Kautsar Abiyoga, Evelyn Komaratih, Yulia Primitasari, Wimbo Sasono, Djoko Legowo. Role of Intravitreal Triamcinolone Acetonide and Bevacizumab in Expression of Matrix Metalloproteinase and Inhibitor in Rabbit Penetrating Injury Model. Research Journal of Pharmacy and Technology 2023; 16(10):4759-6. doi: 10.52711/0974-360X.2023.00772

Citra Dewi Maharani, Nurwasis, Delfitri Lutfi, Clarisa Finanda, Kautsar Abiyoga, Evelyn Komaratih, Yulia Primitasari, Wimbo Sasono, Djoko Legowo. Role of Intravitreal Triamcinolone Acetonide and Bevacizumab in Expression of Matrix Metalloproteinase and Inhibitor in Rabbit Penetrating Injury Model. Research Journal of Pharmacy and Technology 2023; 16(10):4759-6. doi: 10.52711/0974-360X.2023.00772   Available on:

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