Anuradha G. More, Sneha R. Shinde, Padmaja S. Kore, Mayuresh N. Jadhav, Amit S. Tapkir
Anuradha G. More*, Sneha R. Shinde, Padmaja S. Kore, Mayuresh N. Jadhav, Amit S. Tapkir
P.E. Society’s Modern College of Pharmacy, Nigdi, Pune - 411044, Maharashtra, India.
Volume - 16,
Issue - 10,
Year - 2023
The most common type of wound is a burn wound which can be complicated due to electrolyte and fluid loss, leads to increase the chance of infectious complications and delay in wound healing. The main objective of the present study is to develop and evaluate a topical polyherbal ointment for antimicrobial and wound healing activity. The polyherbal ointment was formulated by fusion method using emulsifying ointment base with 10%w/w alcoholic extracts (soxhlet extraction) of Azadirachta indica (Neem), Melaleucaalternifolia (tea tree oil), and Myrica esculenta (katphala). The prepared ointment was evaluated for physicochemical property, wound healing and antimicrobial activity. The prepared ointments were tested for its odor, color, consistency, phase separation, homogeneity, pH, spreadability, viscosity, extrudability, and stability studies and gave satisfactory results. The antimicrobial activity of prepared polyherbalointment was evaluated using standard ATCC strain for gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus) and gram-negative bacteria (Pseudomonas aeruginosa) using the agar welldiffusion method. The polyherbalointment was investigated in vivo in rats for woundhealing activity. The prepared formulation showed predominant antimicrobial activity against selected species and formulation promoted wound healing activity when compared with marketed (standard povidone ointment) preparation. Overall study reveals that prepared formulation is an effective polyherbal ointment.
Cite this article:
Anuradha G. More, Sneha R. Shinde, Padmaja S. Kore, Mayuresh N. Jadhav, Amit S. Tapkir. Investigating the effect of Polyherbal Ointment on Non-Healing Burn Wound: The Geriatric Approach. Research Journal of Pharmacy and Technology 2023; 16(10):4610-6. doi: 10.52711/0974-360X.2023.00751
Anuradha G. More, Sneha R. Shinde, Padmaja S. Kore, Mayuresh N. Jadhav, Amit S. Tapkir. Investigating the effect of Polyherbal Ointment on Non-Healing Burn Wound: The Geriatric Approach. Research Journal of Pharmacy and Technology 2023; 16(10):4610-6. doi: 10.52711/0974-360X.2023.00751 Available on: https://rjptonline.org/AbstractView.aspx?PID=2023-16-10-21
1. K. Karthick, P. Kumaravel, P. Hemalatha, L. Thamaraiselvi. Mechanistic Aspects: Biosynthesis of Silver Nanoparticles from Proteus mirabilis and its Antimicrobial Study. Asian J. Res. Pharm. Sci. 2013; 3( 3): 133-136.
2. Harding K, Patel H. Science, medicine, and the future: healing chronic wounds. British Medical Journal. 2002; 324(7330):160-163. doi:10.1136/bmj.324.7330.160
3. Jaul E. Non healing wounds: The geriatric approach. Archives of Gerontology and Geriatrics. 2008; 49(2009): 224-226.doi: 10.1016/j.archger.2008.08.005
4. Ghosh D, Mondal S, Ramakrishna K. A Topical Ointment Formulation Containing Leaves Extract of Aegialitisrotundifolia roxb., Accelerates Excision, Incision and Burn Wound Healing in Rats. Wound Medicine. 2019; 26(1): 100168. Doi: 10.1016/J.Wndm.2019.100168
5. Ahmed Abduljabbar Jaloob Aljanaby, Israa Abduljabbar Jaloob Aljanaby. Profile of Antimicrobial Resistance of Aerobic Pathogenic Bacteria isolated from Different Clinical Infections in Al-Kufa Central Hospital –Iraq During period from 2015 to 2017. Research J. Pharm. and Tech. 2017; 10(10):3264-3270.
6. Chaturvedi P, Bag A, Rawat V, Jha P. Antibacterial Effects of Azadirachtaindica Leaf and Bark Extracts in Clinical Isolates of Diabetic Patients. NJIRM. 2011 ;2(1):5-9.
7. Gupta A, Ansari S, Gupta S, Narwani M, Gupta M. Therapeutics Role of Neem and Its Bioactive Constituents in Disease Prevention and Treatment. Journal of Pharmacognosy and Phytochemistry. 2019; 8(3):680-691.
8. Kamlesh Singh, Dibyajyoti Saha, Alok Thakur, Ashish Manigauha, Anish Chandy. Aloe Vera Gel for Cure of Alkali Burns. Research J. Pharmacognosy and Phytochemistry. 2011; 3(4): 158-161.
9. Srivastava B, Sharma VC, Pant P, Pandey N, Jadhav A. Evaluation for Substitution of Stem Bark with Small Branches of MyricaEsculenta for Medicinal Use – A Comparative Phytochemical Study. Journal of Ayurveda and Integrative Medicine. 2016;7(4):218-223. Doi: 10.1016/J.Jaim.2016.08.004
10. Kabra A, Martins N, Sharma R, Kabra R, Baghel Us. Myrica Esculenta Buch. -Ham. Ex D. Don: A Natural Source for Health Promotion and Disease Prevention. Plants. 2019; 8(6):149. Doi:10.3390/Plants8060149
11. Sunakar Panda, Swapna Sankar Nayak. Inclusion Complexes of Acridone and Its Semicarbazone Derivative with β- Cyclodextrin: - A Thermodynamic, Spectral and Antimicrobial Study. Asian J. Research Chem. 2009; 2(4): 539-543.
12. Wulansari A, Jufri M, Budianti A. Studies On the Formulation, Physical Stability, And in Vitro Antibacterial Activity of Tea Tree Oil (MelaleucaAlternifolia) Nanoemulsion Gel. International Journal of Applied Pharmaceutics. 2017; 9:135. Doi:10.22159/Ijap.2017.
13. Sayeed ul hasan S.M, Nitin Mahurkar. Evaluation of Wound Healing Effect of Polyherbal Ointment in Alloxan Induced Diabetic Rats. Research J. Topical and Cosmetic Sci. 2015; 6(1): 38-43.
14. S. Priya, S. Nethaji. Antimicrobial study of leaves and bark extracts of Diospyros virginiana. Res. J. Pharmacognosy & Phytochem. 2014; 6(1): 16-18.
15. Deshpande M.M., Seema I. Habib, Deshpande V. G., Praffullkumar A Kulkarni. Synthesis Characterization and Antimicrobial Evaluation of Some New Schiff Bases. Asian J. Research Chem. 2013; 6(2): 131-134.
16. Jifi Antony, Anitha Victoria Noronha, Sheela Williams, Janet Mathias, Usha Thomas. A study to assess the effectiveness of Banana Leaf Dressing (BLD) among patients with partial thickness burns in selected hospitals at Karnataka. Asian J. Nur. Edu. & Research. 2014; 4(1): 15-19.
17. Charde Y, Sharma P, Choudhary N, Avari J. Development and Evaluation of Herbal Formulation for The Treatment of Acne. International Journal of Pharmaceutical Sciences and Research. 2014;5(6):22502260. Doi:10.13040/Ijpsr.09758232.5(6).2250-60
18. Agnihotri S, Wakode S, Agnihotri A. Formulation and Evaluation of Herbal Antiacne Gel of Myrica Esculenta. Asian Journal of Pharmaceutical and Clinical Research. 2016;9(4):358-361.
19. Silpa M, Suresh Joghee, Hamsalakshmi. Formulation and Evaluation of Antimicrobial Ointments from Eupatorium Glandulosum Hort. Ex Kunth. Asian Journal of Pharmaceutical and Clinical Research. 2019:224-228. Doi:10.22159/Ajpcr. 2020.V13i1.29533
20. Maru AD, Lahoti SR. Formulation and evaluation of ointment containing sunflower wax. Asian Journal of Pharmaceutical and Clinical Research. 2019;12(8):116-120. doi:10.22159/ajpcr. 2019.v12i18.33199
21. Shukla R, Kashaw V. Development, Characterization and Evaluation of Herbal Ointment Containing Momordicacharantia, PongamiaGlabra and Piper Nigrum. Journal of Drug Delivery and Therapeutics. 2019;9(1):136-140. Doi:10.22270/Jddt.V9i1.2190
22. C Shaikh S, Sanap D, Bhusari DV, Jain S, Kochar PP, Memon FS. Fabrication and Evaluation of Herbal Ointment Formulations of Moringa Olifera for Topical Delivery. Universal Journal of Pharmaceutical Research. 2018. Doi:10.22270/Ujpr.V3i4.181
23. Chibuzo UC. Antimicrobial Activity of Azadirachta indica (Neem) Leaf Extract On Some Bacteria. International Journal of Current Microbiology and Applied Sciences. 2019; 8(7):431-437. Doi:10.20546/Ijcmas.2019.807.053
24. Mateen A, Parwez A. A Study of Antimicrobial Activity of Few Medicinally Important Herbal Single Drugs Extracted in Ethanol, Methanol and Aqueous Solvents. Pharmacognosy Journal. 2013;2(10):351-356. doi:10.1016/S0975-3575(10)80108-7
25. Dev SK, Choudhury P, Srivastava R, Sharma M. Antimicrobial, Anti-Inflammatory and Wound Healing Activity of Polyherbal Formulation. Biomedicine & Pharmacotherapy. 2019; 111:555-567. Doi: 10.1016/J.Biopha.2018.12.075
26. Said A, Wahid F. Sauromatum guttatum extract promotes wound healing and tissue regeneration in a burn mouse model via up-regulation of growth factors. Pharmaceutical Biology. 2019; 57:736-743. doi.org/10.1080/13880209.2019.1676266.
27. Toppo, F.A. and Pawar, R.S. Appraisal on the wound healing activity of different extracts obtained from Aegle marmelos and Mucunapruriens by in vivo experimental models. Nigerian Journal of Clinical Practice. 2016; 19(6), pp.753-760
28. Elzayat EM, Auda SH. Evaluation of wound healing activity of henna, pomegranate and myrrh herbal ointment blender. Saudi Pharmaceutical Journal. 2018;26(5):733-738. doi.org/10.1016/j.jsps.2018.02.016.
29. Kamini P. Sao. Knowledge regarding prevention and First Aid for burns among parents of under five children. Int. J. Nur. Edu. and Research. 2017; 5(3): 269-271.
30. Fakhir Daffa Fakhir, Imad Hadi Hameed, Salam Salah Flayyih. Burns Injuries: A Prospective Statistical Study of 112 patients. Research J. Pharm. and Tech. 2017; 10(10):3401-3407.