Author(s):
Fauziah Pratiwi, Alpha Fardah Athiyyah, Andy Darma, Reza Gunadi Ranuh, Widjiati Widjiati, Wibi Riawan, Khadijah Rizky Sumitro, SubijantoMarto Sudarmo
Email(s):
alpha-f-a@fk.unair.ac.id
DOI:
10.52711/0974-360X.2023.00055 
Address:
Fauziah Pratiwi, Alpha Fardah Athiyyah*, Andy Darma, Reza Gunadi Ranuh, Widjiati Widjiati, Wibi Riawan, Khadijah Rizky Sumitro, SubijantoMarto Sudarmo
Department of Child Health, Dr. Soetomo Hospital, Faculty of Medicine, Universitas Airlangga, Jl. Prof. Dr.Moestopo No. 6-8, Surabaya, Indonesia.
*Corresponding Author
Published In:
Volume - 16,
Issue - 1,
Year - 2023
ABSTRACT:
Gastric injury is an event that often occurs due to many factors, such as the use of drugs, stress factors, infections, chemicals, etc. The use of histamin 2(H2) receptor antagonist drugs and pump inhibitors have become the choice for gastric injury treatment so far and requires a relatively long time. The widespread use of probiotics has been shown to affect the healing process of digestive tract disorders, for example in the small intestine. This study aimed to investigate the effect of Lactobacillus plantarumIS-10506 in acceleratingthe healing of gastric injury induced by ketorolac in the rat. The experimental study used 64 Wistar rats divided into 4 groups, group 1 (control), group 2(ketorolac administration), group 3 (ketorolac and probiotic administration), and group 4(preventive treatment with probiotic before, ketorolac administration, and treatment with probiotic). Each group was divided into 4 subgroups based on the day of sacrifice, days 1, 5, 7, 10. The healing of gastric injury evaluating by epithelial defects improvement and fibroblast cells by hematoxylin and eosin (HE) staining.The group induced by ketorolac (group 2) showed the highest epithelial defect score (p=0.048) on day 1. The repair of the epithelial defect in group 3 and group 4 were significantly increased on day 5, while group 2remains defectiveon day 5(p=0.019). Fibroblast cells of groups 3 and 4 decreased significantly more than others on day 10(p=0.024). Lactobacillus plantarum IS-10506 influences the healing acceleration of gastric injury by ketorolac by enhancing epithelial regenerationand fibroblast cells.
Cite this article:
Fauziah Pratiwi, Alpha Fardah Athiyyah, Andy Darma, Reza Gunadi Ranuh, Widjiati Widjiati, Wibi Riawan, Khadijah Rizky Sumitro, SubijantoMarto Sudarmo. Lactobacillus plantarum IS-10506 Accelerates Healing of Gastric Injury Induced by Ketorolac in Wistar Rats. Research Journal of Pharmacy and Technology 2023; 16(1):307-3. doi: 10.52711/0974-360X.2023.00055
Cite(Electronic):
Fauziah Pratiwi, Alpha Fardah Athiyyah, Andy Darma, Reza Gunadi Ranuh, Widjiati Widjiati, Wibi Riawan, Khadijah Rizky Sumitro, SubijantoMarto Sudarmo. Lactobacillus plantarum IS-10506 Accelerates Healing of Gastric Injury Induced by Ketorolac in Wistar Rats. Research Journal of Pharmacy and Technology 2023; 16(1):307-3. doi: 10.52711/0974-360X.2023.00055 Available on: https://rjptonline.org/AbstractView.aspx?PID=2023-16-1-55
REFERENCES:
1. Russo R, Caro C De, Avallone B, Magliocca S, Nieddu M, Boatto G, et al. Ketogal: A derivative ketorolac molecule with minor ulcerogenic and renal toxicity. Front Pharmacol. 2017;8(NOV):1–11.
2. Rodríguez-Silverio J, Sánchez-Mendoza ME, Arrieta-Valencia J, Rocha-Gonzalez HI, Flores-Murrieta FJ. Tizanidine increases antinociceptive effect and prevents gastric damage induced by ketorolac in the rat. Drug Dev Res. 2013;74(1):38–42.
3. Rao TD, Kumar MPS. Analgesic Efficacy of Paracetamol Vs Ketorolac after Dental Extractions. Res J Pharm Tech. 2018;11(8):3375–9.
4. Tarnawski AS, Ahluwalia A. The critical role of growth factors in gastric ulcer healing: The cellular and molecular mechanisms and potential clinical implications. Cells. 2021;10(8).
5. Gomi A, Kano M, Miyazaki K, Ishikawa F. Effect of Bifidobacterium bifidum BF-1 on gastric protection and mucin production in an acute gastric injury rat model. J Dairy Sci. 2013;96(2):832–7.
6. Manimekalai P, Maheshwari P, Velmurugan R, Gurumoorthy M, Kumar SH, Vijayakumar G. Gastro Protective effect of Standardized Ethanolic leaf extract of Indigofera tinctoriao on experimental Gastric Ulcers in Rats. Res J Pharm Tech. 2018;11(2):527–31.
7. Narkhede KP, Satapathy T, Pandit B. Protective effect of Cod Liver Oil in Experimentally Induced Gastric Ulceration in Rats. Res J Pharm Tech. 2019;12(1):5–10.
8. Muthukumaran P, Pattabiraman K. Anti-Ulcer Effects of Ipomoea aquatica forsk Leaves against Gastric Ulcers in Rats. Res J Pharmacogn Phytochem. 2010;2(6):468–71.
9. Khoder G, Al-Menhali AA, Al-Yassir F, Karam SM. Potential role of probiotics in the management of gastric ulcer (Review). Exp Ther Med. 2016;12(1):3–17.
10. Melgar-Lalanne G, Rivera-Espinoza Y, Hernández-Sánchez H. Lactobacillus plantarum: An overview with emphasis in biochemical and healthy properties. Lact Classif Uses Heal Implic. 2012;(September 2012):1–34.
11. Athiyyah AF, Darma A, Ranuh RG, Riawan W, Sudiana IK, Surono IS, et al. The effects of lactobacillus plantarum IS-10506 on accelerating the regeneration of the intestinal mucosa structure examined by the scanning electron microscope. Carpathian J Food Sci Technol. 2019;10(5):11–8.
12. Ranuh RG, Athiyyah AF, Darma A, Riawan W, Surono IS, Sandra F, et al. Lactobacillus plantarum IS-20506 probiotic restores galectin-4 and myosin-1a expressions in duodenum, jejunum and ileum of lipopolysaccharide-induced rats. Indones Biomed J. 2020;12(3):283–7.
13. Rogers AB. Histologic scoring of gastritis and gastric cancer in mouse models. Methods Mol Biol. 2012;921(1):189–203.
14. Nitin M, Mohan Reddy U, Sayeed Ul Hasan S. Pharmacodynamic influence of vitamin C and Esomeprazole on gastro protection in pylorus ligation and aspirin induced ulcers in rats. Res J Pharmacol Pharmacodyn. 2012;4(3):176–80.
15. Saini S, Majee C. Antiulcer activity of Lagerstroemia indica leaves in Indomethacin Induced Gastric Ulcer in Rats. Res J Pharm Tech. 2021;14(8):4408–12.
16. Yuvaraj S, Priya AJ, Sriram N. Effect of Non Steroidal Anti Inflammatory Drugs in Pregnancy: A Systematic Review. Res J Pharm Tech. 2015;8(6):787–91.
17. Wallace JL, McKnight W, Reuter BK, Vergnolle N. NSAID-Induced gastric damage in rats: Requirement for inhibition of both cyclooxygenase 1 and 2. Gastroenterology. 2000;119(3):706–14.
18. Rocha-González HI, Sánchez-Mendoza ME, Cruz-Antonio L, Flores-Murrieta FJ, Cornelio-Huerta XI, Arrieta J. Antinociceptive Interaction and Pharmacokinetics of the Combination Treatments of Methyleugenol Plus Diclofenac or Ketorolac. Molecules [Internet]. 2020 Nov 3 [cited 2022 Jul 1];25(21). Available from: https://pubmed.ncbi.nlm.nih.gov/33153182/
19. Manikandan A, Doss DVA. Antiulcer Activity of 50% Hydroethanolic Leaf Extract of Ruellia tuberosa l. and Dipteracanthus patulus (Jaca) On Pylorus Ligated Gastric Ulcer Model. Res J Pharmacogn Phytochem. 2009;1(3):198–200.
20. Wallace JL. NSAID gastropathy and enteropathy: Distinct pathogenesis likely necessitates distinct prevention strategies. Br J Pharmacol. 2012;165(1):67–74.
21. Singh K, Monika, Verma N. Gastrointestinal and Cardiovascular Risks of Non-Steroidal AntiInflammatory Drugs (NSAIDs): Time to Translate Knowledge into Practice. Res J Pharm Tech. 2014;7(5):575–80.
22. Shirode DS, Powar P, Jain BB, Agarwal A. Antiulcer effect of Blumea lacera against Gastric ulcers in rats. Res J Pharm Tech. 2020;13(7):3340–2.
23. Wallace JL. Prostaglandins, NSAIDs, and gastric mucosal protection: Why doesn’t the stomach digest itself? Physiol Rev. 2008;88(4):1547–65.
24. Fornai M, Antonioli L, Colucci R, Tuccori M, Blandizzi C. Pathophysiology of Gastric Ulcer Development and Healing : Molecular Mechanisms and Novel Therapeutic Options. 2008;
25. Uchida M, Kurakazu K. Yogurt containing Lactobacillus gasseri OLL2716 exerts gastroprotective action agaisnt acute gastric lesion and antral ulcer in rats. J Pharmacol Sci. 2004;96(1):84–90.
26. Murali Eswara S, Kavitha BTVV, Srikanth JG, Velmani G. Probiotics as potential therapies in human gastrointestinal health. Int J Adv Pharm Sci. 2010;1(2):96–110.
27. Yan F, Polk DB. Probiotics and immune health. Curr Opin Gastroenterol [Internet]. 2011 Oct [cited 2022 Jul 1];27(6):496–501. Available from: https://pubmed.ncbi.nlm.nih.gov/21897224/
28. Sullivan Å, Nord CE. Probiotics and gastrointestinal diseases. J Intern Med. 2005;257(1):78–92.
29. Resta-Lenert S, Barrett KE. Live probiotics protect intestinal epithelial cells from the effects of infection with enteroinvasive Escherichia coli (EIEC). Gut. 2003;52(7):988–97.
30. Valdéz JC, Peral MC, Rachid M, Santana M, Perdigón G. Interference of Lactobacillus plantarum with Pseudomonas aeruginosa in vitro and in infected burns: The potential use of probiotics in wound treatment. Clin Microbiol Infect. 2005;11(6):472–9.
31. Halper J, Leshin LS, Lewis SJ, Li WI. Wound Healing and Angiogenic Properties of Supernatants from Lactobacillus Cultures: https://doi.org/101177/153537020322801111 [Internet]. 2016 Nov 29 [cited 2022 Jul 3];228(11):1329–37. Available from: https://journals.sagepub.com/doi/10.1177/153537020322801111
32. Ogueke CC, Owuamanam CI, Ihediohanma NC, Iwouno JO. Probiotics and prebiotics: Unfolding prospects for better human health. Pakistan J Nutr. 2010;9(9):833–43.
33. Pathmakanthan S, Li CKF, Cowie J, Hawkey CJ. Lactobacillus plantarum 299: Beneficial in vitro immunomodulation in cells extracted from inflamed human colon. J Gastroenterol Hepatol. 2004;19(2):166–73.
34. Ng SC, Hart AL, Kamm MA, Stagg AJ, Knight SC. Mechanisms of action of probiotics: recent advances. Inflamm Bowel Dis [Internet]. 2009 [cited 2022 Jul 1];15(2):300–10. Available from: https://pubmed.ncbi.nlm.nih.gov/18626975/
35. Quigley EMM. Prebiotics and Probiotics in Digestive Health. Clin Gastroenterol Hepatol. 2019;17(2):333–44.
36. Nasrabadi MH, Aboutalebi H, Ebrahimi MT, Zahedi F. The healing effect of Lactobacillus plantarum isolated from Iranian traditional cheese on gastric ulcer in rats. African J Pharm Pharmacol. 2011;5(12):1446–51.
37. Playford RJ, Wright NA. Peptides and peptic ulceration. QJM - Mon J Assoc Physicians. 1998;91(10):661–5.
38. Mori Y, Hashimoto S, Watanabe T, Yokokura T. Anti-ulcer effects of lactic acid bacteria and their cell wall polysaccharides. Biol Pharm Bull [Internet]. 1994 [cited 2022 Jul 1];17(8):1012–7. Available from: https://pubmed.ncbi.nlm.nih.gov/7820099/