Author(s):
Ni Komang Widiastuti, Ni Made Virginia, I Made Fery Yastawan
Email(s):
putripermatasari@undhirabali.ac.id
DOI:
10.52711/0974-360X.2023.00028 
Address:
Ni Komang Widiastuti1, Ni Made Virginia1, I Made Fery Yastawan1,
Anak Agung Ayu Putri Permatasari1*, Putu Angga Wiradana1, I Gede Widhiantara1, Teguh Hari Sucipto2
1Study Program of Biology, Faculty of Health, Science, and Technology, University of Dhyana Pura, Kuta Utara 80361, Badung, Bali, Indonesia.
2Dengue Study Group, Institute of Tropical Disease, Universitas Airlangga, Kampus C, Mulyorejo, Surabaya 60286, East Java, Indonesia.
*Corresponding Author
Published In:
Volume - 16,
Issue - 1,
Year - 2023
ABSTRACT:
In several societies, the development of traditional medicine employing plants to enhance medicinal formulations has been promoted as a valuable offering in therapeutic applications. Bali Province in Indonesia, which has a healthy culture until now, has a rich history of traditional medicine regarding the utilization of local plants. The purpose of this study was to determine the cytotoxicity of Erythrina lithosperma Miq leaf extract (ELLE) on Vero cells to gain knowledge of its potential as a standardized traditional medicine. A cytotoxicity test was performed on Vero cell lines grown on M119 medium with 10% FBS and incubated at 37°C in a CO2 incubator until confluent. Cells were collected and grown in 96 well microplates at a cell density of 2x104 cells/100mL/well at the start. After a 24-hour incubation period, extracts were subjected to concentrations ranging from 31.25 to 10,000µg/ml. Cell viability was determined using the MTT technique (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide). A one-way ANOVA test with a significance level of 5% was performed. Cell viability increased with decreasing concentration extracts used. There was no significant difference between concentrations of 500, 250, 125, 63, and 31µg/ml. A consistent and significant reduction occurred in cell proliferation added with very high extract concentrations. Empirically ELLE can be used as a traditional medicine for certain diseases, this experiment reduces the viability of Vero cells lines at very high concentrations. Overall, the findings of this study suggest that the investigated ELLE might be developed as a novel dietary component and traditional medicinal preparation for promoting human health.
Cite this article:
Ni Komang Widiastuti, Ni Made Virginia, I Made Fery Yastawan. Cytotoxicity Evaluation of Erythrina lithosperma Miq. Leaf Extract against Vero Cell Lines: In Vitro Study. Research Journal of Pharmacy and Technology 2023; 16(1):153-8. doi: 10.52711/0974-360X.2023.00028
Cite(Electronic):
Ni Komang Widiastuti, Ni Made Virginia, I Made Fery Yastawan. Cytotoxicity Evaluation of Erythrina lithosperma Miq. Leaf Extract against Vero Cell Lines: In Vitro Study. Research Journal of Pharmacy and Technology 2023; 16(1):153-8. doi: 10.52711/0974-360X.2023.00028 Available on: https://rjptonline.org/AbstractView.aspx?PID=2023-16-1-28
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