Author(s): Swapna. Vemireddy, Gandla Kumaraswamy, B. Jyothsna, M. Bindu

Email(s): drkumaraswamygandla@gmail.com

DOI: 10.52711/0974-360X.2023.00020   

Address: Swapna. Vemireddy1, Gandla Kumaraswamy2*, B. Jyothsna3, M. Bindu4
1Research Scholar, Chaitanya (Deemed to be University), Department of Pharmacy, Warangal, Telangana - 506001, India.
2Department of Pharmaceutical Analysis, Chaitanya (Deemed to be University), Hanumakonda, Telangana - 506001, India.
3Chilkur Balaji College of Pharmacy, Aziz Nagar, Rangareddy, Telangana, 500075, India.
4Marri Laxman Reddy (MLR) Institute of Pharmacy, Dundigal (Mandal), Medchal (District), Hyderabad - 500043, Telangana.
*Corresponding Author

Published In:   Volume - 16,      Issue - 1,     Year - 2023


ABSTRACT:
A novel approach was used to develop and validate a rapid, specific, accurate and precise reverse phase high performance liquid chromatographic (HPLC) method for the estimation of Guaifenesin in bulk and pharmaceutical dosage forms. The chromatographic separation was achieved by Cosmosil packed (5C18-MS-II, 4.6 internal diameter 250mm lengths) column, using mobile phase ratio of Acetonitrile: Phosphate buffer PH 5(70:30). The flow rate was 1mL/min and the detection wavelength was 260nm. The limit of detection (LOD) for guaifenesin was 0.23µg/ml and the limit of quantification (LOQ) for guaifenesin was 0.78µg/ml. This method was validated concerning linearity, accuracy, precision, specificity and robustness. The method was also found to be stability-indicating.


Cite this article:
Swapna. Vemireddy, Gandla Kumaraswamy, B. Jyothsna, M. Bindu. A Validated RP-HPLC Method for Quantitative Estimation of Guaifenesin in Bulk and Pharmaceutical Dosage Forms. Research Journal of Pharmacy and Technology 2023; 16(1):111-4. doi: 10.52711/0974-360X.2023.00020

Cite(Electronic):
Swapna. Vemireddy, Gandla Kumaraswamy, B. Jyothsna, M. Bindu. A Validated RP-HPLC Method for Quantitative Estimation of Guaifenesin in Bulk and Pharmaceutical Dosage Forms. Research Journal of Pharmacy and Technology 2023; 16(1):111-4. doi: 10.52711/0974-360X.2023.00020   Available on: https://rjptonline.org/AbstractView.aspx?PID=2023-16-1-20


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