Author(s): Safaa Hadi Hussein, Suhad Faisal Hatem Al-Mugdadi, Lec. Ali Jalil Mjali, Qabas Nather Latef, Zahraa Ahmed Okhti

Email(s): zahraaahmedokhti1981@gmail.com

DOI: 10.52711/0974-360X.2023.00016   

Address: Safaa Hadi Hussein1, Suhad Faisal Hatem Al-Mugdadi2, Lec. Ali Jalil Mjali3, Qabas Nather Latef2, Zahraa Ahmed Okhti2
1Teaching Laboratories, Medical City, Baghdad, Iraq.
2Department of Clinical Laboratories Sciences, College of Pharmacy, Mustansiriyah University, Baghdad, Iraq.
3Department of Chemistry, College of Science, Mustansiriyah University, Baghdad, Iraq.
*Corresponding Author

Published In:   Volume - 16,      Issue - 1,     Year - 2023


ABSTRACT:
Background: Widespread usage of antimicrobial drugs has increased the emergence of antibiotic-resistant pathogens and that's why we need new drugs. Lignin and its derivatives considered as an antioxidant, anti-inflammatory, insecticidal and antimicrobial. This study aimed to investigate the effect of lignin polymer against the bacteria isolated from clinical samples, and to study the antibiotic resistance pattern using the Vitek system. Materials and Methods: 50 clinical bacterial samples were collected from two hospitals in Baghdad city. All the isolates of Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus were subjected to Vitek system to determine the resistance for 12 antibiotics. The Soda lignin polymer had been prepared from the palm tree empty fruit/ Malaysia. The biological activity of lignin polymer against resistant bacteria was evaluated by well agar diffusion method. Results: According to the Vitek system, all the bacterial isolates were multi-resistance to many antibiotics. Lignin polymer dilutions inhibited the growth of some bacterial isolates, and it was more effective in all the concentrations with a good inhibition zone on Pseudomonas aeruginosa which reached to 20mm. Conclusion: Lignin polymer has an antibacterial effect against some pathogenic multidrug resistant bacteria isolated from clinical samples, including urine and wound infection. Lignin polymer was more effective in all the concentrations on the isolates of Pseudomonas aeruginosa, with a good inhibition zone.


Cite this article:
Safaa Hadi Hussein, Suhad Faisal Hatem Al-Mugdadi, Lec. Ali Jalil Mjali, Qabas Nather Latef, Zahraa Ahmed Okhti. Antibacterial effect of Lignin Polymer on Multidrug Resistant Bacteria Identified by Vitek System. Research Journal of Pharmacy and Technology 2023; 16(1):91-6. doi: 10.52711/0974-360X.2023.00016

Cite(Electronic):
Safaa Hadi Hussein, Suhad Faisal Hatem Al-Mugdadi, Lec. Ali Jalil Mjali, Qabas Nather Latef, Zahraa Ahmed Okhti. Antibacterial effect of Lignin Polymer on Multidrug Resistant Bacteria Identified by Vitek System. Research Journal of Pharmacy and Technology 2023; 16(1):91-6. doi: 10.52711/0974-360X.2023.00016   Available on: https://rjptonline.org/AbstractView.aspx?PID=2023-16-1-16


REFERENCES:
1.    Lee SJ, Lee SJ, Lee SK, Yoon HJ, Lee HH, Kim KK, Lee BJ, Lee BI, Suh SW. Structures of Staphylococcus aureus peptide deformylase in complex with two classes of new inhibitors. Acta Crystallographica Section D: Biological Crystallography. 2012 Jul 1;68(7):784-93.
2.    Ananthula RS, Ravikumar M, Mahmood SK, Kumar MP. Insights from ligand and structure based methods in virtual screening of selective Ni-peptide deformylase inhibitors. Journal of molecular modeling. 2012 Feb 1;18(2):693-708.
3.    Zaman SB, Hussain MA, Nye R, Mehta V, Mamun KT, Hossain N. A review on antibiotic resistance: alarm bells are ringing. Cureus. 2017 Jun;9(6):e1403.
4.    Dimitrov TS, Udo EE, Emara M, Awni F, Passadilla R. Etiology and antibiotic susceptibility patterns of community-acquired urinary tract infections in a Kuwait hospital. Medical principles and Practice. 2004;13(6):334-9.
5.    Astal ZE. Increasing ciprofloxacin resistance among prevalent urinary tract bacterial isolates in the Gaza Strip. Singapore medical journal. 2005 Sep 1;46(9):457.
6.    Tsay RW, Siu LK, Fung CP, Chang FY. Characteristics of bacteremia between community-acquired and nosocomial Klebsiella pneumoniae infection: risk factor for mortality and the impact of capsular serotypes as a herald for community-acquired infection. Archives of internal medicine. 2002 May 13;162(9):1021-7.
7.    Chambers H.F. Community-associated MRSA-resistance and virulence converge. N Engl J Med 2005; 352(14): 1485- 1487
8.    Javiya VA, Ghatak SB, Patel KR, Patel JA. Antibiotic susceptibility patterns of Pseudomonas aeruginosa at a tertiary care hospital in Gujarat, India. Indian journal of pharmacology. 2008 Oct;40(5):230.
9.    Kumar AR. Antimicrobial Sensitivity Pattern of Staphylococcus aureus isolated from Pus From tertiary Care Hospital, Surendranagar, Gujarat and Issues Related to the Rational Selection of Antimicrobials. Sch JApp Med Sci 2013; 1(5): 600-605.
10.    Salmond GP, Welch M. Antibiotic resistance: adaptive evolution. The Lancet. 2008 Dec 1;372:S97-103.
11.    Sikarwar AS, Batra HV. Prevalence of antimicrobial drug resistance of Klebsiella pneumoniae in India. International Journal of Bioscience, Biochemistry and Bioinformatics. 2011 Sep 1;1(3):211.
12.    Magiorakos AP, Srinivasan A, Carey RT, Carmeli Y, Falagas MT, Giske CT, Harbarth S, Hindler JT, Kahlmeter G, Olsson-Liljequist B, Paterson DT. Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clinical microbiology and infection. 2012 Mar 1;18(3):268-81.
13.    Michelle K. P and Joan M. Klebsiella pneumoniae: Going on the Offense with a Strong Defense. Microbiol Mol Biol Rev. 2016 Sep; 80(3): 629–661.
14.    Motbainor H, Bereded F, Mulu W. Multi-drug resistance of blood stream, urinary tract and surgical site nosocomial infections of Acinetobacter baumannii and Pseudomonas aeruginosa among patients hospitalized at Felegehiwot referral hospital, Northwest Ethiopia: a cross-sectional study. BMC Infectious Diseases. 2020 Dec 1;20(1):92.
15.    Arias CA and Murray BE. “Antibiotic-resistant bugs in the 21st century a clinical super-challenge,” The New England Journal of Medicine. 2009 ; 360( 5): 439–443.
16.    Sarah AH, Ali MN, Kasim SA. Isolation and identification of multi-drug resistant “pseudomonas aeruginosa” from burn wound infection in Kirkuk City, Iraq. Eurasia J Biosci. 2019; 13:1045-1050.
17.    Imad SM, Khalil IA, Mohammad KAS, Safa D, Nahla NA. Determination of Antimicrobial Drug Resistance among Bacterial Isolates in Two Hospitals of Baghdad. Jordan Journal of Pharmaceutical Sciences. 2020; 13(1):1-9.
18.    Chiavari-Frederico MO, Barbosa LN, Carvalho dos Santos I, Ratti da Silva G, Fernandes de Castro A, de Campos Bortolucci W, Barboza LN, Campos CF, Gonçalves JE, Menetrier JV, Jacomassi E. Antimicrobial activity of Asteraceae species against bacterial pathogens isolated from
19.    Santos PR, Oliveira AC, Tomassini TC. Controle microbiológico de produtos fitoterápicos. Rev. farm. bioquim. Univ. Säo Paulo. 1995; 31: 35-38.
20.    Santos Filho D, Sarti SJ, Bastos JK, Leitäo Filho HD, Machado JO, Araújo ML, Lopes WD, Abreu JE. Atividade antibacteriana de extratos vegetais. Rev. ciênc. farm. 1990; 12: 39-46.
21.    Hassan T Abdulsahib, Abdulamir H Taobi, Salah S Hashem. A Novel Adsorbent Based on Lignin and Tannin for the Removal of Heavy Metals from Wastewater. Res. J. Pharmacognosy and Phytochem. 2015; 7(1): 38-48.
22.    Saiprabha M. Mahale, Anita S. Goswami-Giri. Development of Renewable Matrix (Lignin) from Mango Wastes and It’s FT-IR Spectroscopic Prediction. Asian J. Research Chem. 4(10): Oct., 2011; Page 1635-1637.
23.    S. Natarajan, R. Sukanya Devi, R. Surya. Development of Eco-friendly water repellent fabrics. Research J. Engineering and Tech. 2017; 8(4): 389-392.
24.    Fatima Zohra Bentebba, Ghiaba Zineb, Mokhtar Saidi. Phytochemical content and Antioxidant properties of Ghars date palm (Phoenix dactylifera L.) harvested at different stages of maturity. Asian J. Research Chem. 2020; 13(5):323-326.
25.    Messaoudi Abdeldjabbar, Dekmouche Messaouda, Rahmani Zhour, Bensaci Cheyma. Comparative Analysis of Total Phenolics, Flavonoid content and Antioxidant profile of date palm (Phoenix dactylifera L.) with different watering water from Oued Soufin Algeria; Asian J. Research Chem. 2020; 13(1): 28-32.
26.    Slavikova E, Kosikova B. Current awareness on yeast. Yeast. 1994;11(3):293-300.
27.    Lebo SE. JR, Gargulak JD, McNally TJ. Kirk Othmer Encyclopedia of Chemical Technology.2008;232:81-89.
28.    Cheyma Bensaci, Zineb Ghiaba, Messaouda Dakmouche, Mokhtar Saidi, Mohamed Hadjadj, Assia Belfar, Mahdi Belguidoum. Studies on Antibacterial Compounds from Methanolic Extracts of Date Palm (Phoenix dactylifera L.) fruits from Algeria. Asian J. Research Chem. 2019; 12(6):338-340.
29.    Ahmed Kareem Obaid Aldulaimi, Ameer Hassan idan, Ahmed Habeeb Radhi, Saadon Abdulla Aowda, Saripah Salbiah Syed Abdul Azziz, Wan Mohd Nuzul Hakimi Wan Salleh, Tamara Kareem Obaid Aldulaimi, Mailina Jamil, Mohd Shafik Yuzman, Nor Azah Mohamad Ali. GCMS Analysis and Biological Activities of Iraq Zahdi Date Palm Phoenix dactylifera L Volatile Compositions. Research J. Pharm. and Tech. 2020; 13(11):5207-5209.
30.    Nada AM, El‐Diwany AI, Elshafei AM. Infrared and antimicrobial studies on different lignins. Acta biotechnologica. 1989;9(3):295-8.
31.    Monika Verma, Amia Ekka. Decolorization and Degradation of Kraft Lignin Discharged from Pulp and Paper Mill Industry by Axenic and Co-Culture of Bacillus sp. Research J. Pharm. and Tech. 2018; 11(10): 4386-4392.
32.    Ibrahim M, Nor Nadiah MYN, Azian H. Comparison studies between soda lignin and soda-anthraquinone lignin in terms of physico-chemical properties and structural features. JApSc. 2006;6(2):292-6.
33.    Ibrahim A, Ibrahim MM, Rahim AA. Corrosion inhibition of mild steel in near neutral solution by kraft and soda lignins extracted from oil palm empty fruit bunch. Int. J. Electrochem. Sci. 2011 Nov 1;6(11):5396-416.
34.    Al-Hasnawy HH, Judi MR, Hamza HJ. The Dissemination of Multidrug Resistance (MDR) and Extensively Drug Resistant (XDR) among Uropathogenic E. coli (UPEC) Isolates from Urinary Tract Infection Patients in Babylon Province, Iraq. Baghdad Science Journal. 2019;16(4 Supplement):986-22.
35.    Freshinta Jellia Wibisono, Bambang Sumiarto, Tri Untari, Mustofa Helmi Effendi, Dian Ayu Permatasari, and Adiana Mutamsari Witaningrum. Resistance Profile of Extended Spectrum Beta Lactamase-Producing Escherichia coli Bacteria using Vitek® 2 Compact Method. Buletin Peternakan May 2020; 44 (2): 48-53.
36.    Shariff, AR, Shenoy S, Yadav T, Radhakrishna M. The Antibiotic Susceptibility Patterns of Uropathogenic Escherichia Coli, with Special Reference to the Fluoroquinolones. J Clin Diagn Res. 2013; (7)6 :1027-1030.
37.    Horcajada JP, Montero M, Oliver A, Sorlí L, Luque S, Gómez-Zorrilla S, Benito N, Grau S. Epidemiology and treatment of multidrug-resistant and extensively drug-resistant Pseudomonas aeruginosa infections. Clinical microbiology reviews. 2019 Sep 18;32(4):e00031-19.
38.    Lee YD, Park JH. Phage conversion for β-lactam antibiotic resistance of Staphylococcus aureus from foods. J. Microbiol. Biotechnol. 2016 Feb 1;26(2):263-9.
39.    Peleg AY, Hooper DC. Hospital-acquired infections due to Gram-negative bacteria. N.Engl.J.Med. 2010;362,1804–1813.
40.    Shankar S, Rhim JW. Preparation and characterization of agar/lignin/silver nanoparticles composite films with ultraviolet light barrier and antibacterial properties. Food Hydrocolloids. 2017 Oct 1;71:76-84.
41.    Li M, Jiang X, Wang D, Xu Z, Yang M. In situ reduction of silver nanoparticles in the lignin based hydrogel for enhanced antibacterial application. Colloids and Surfaces B: Biointerfaces. 2019 May 1;177:370-6.
42.    Gan D, Xing W, Jiang L, Fang J, Zhao C, Ren F, Fang L, Wang K, Lu X. Plant-inspired adhesive and tough hydrogel based on Ag-Lignin nanoparticles-triggered dynamic redox catechol chemistry. Nature communications. 2019 Apr 2;10(1):1-0.
43.    Yang W, Fortunati E, Gao D, Balestra GM, Giovanale G, He X, Torre L, Kenny JM, Puglia D. Valorization of acid isolated high yield lignin nanoparticles as innovative antioxidant/antimicrobial organic materials. ACS Sustainable Chemistry and Engineering. 2018 Jan 11;6(3):3502-14.
44.    Panel W.Yang E.Fortunati F.Dominici G. Giovanale A. Mazzaglia G.M Balestra J.M.Kenny D.Puglia. Effect of cellulose and lignin on disintegration, antimicrobial and antioxidant properties of PLA active films. International Journal of Biological Macromolecules. 2016; 89: 360-368.
45.    Venkateswarulu, T. C., et al. "Studies on Electrophoretic Band Pattern of Isozymes in all Leaves of Pisifera Variety of Oil Palm (Elaeis guineensis Jacq)." Research Journal of Pharmacy and Technology. 2014; 7(4): 415-418.

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

1.3
2021CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank

Journal Policies & Information


Recent Articles




Tags


Not Available