K. Ashok Kumar, Firoj A. Tamboli, Harinath N. More, Kamal M. Alaskar, Prashant G. Tandale
K. Ashok Kumar1, Firoj A. Tamboli2*, Harinath N. More2, Kamal M. Alaskar3, Prashant G. Tandale3
1Vignan Institute of Pharmaceutical Sciences, Deshmukhi, Hyderabad, India.
*2Department of Pharmacognosy, Bharati Vidyapeeth College of Pharmacy, Near Chitranagari, Kolhapur - 416013 Maharashtra, India.
3Bharati Vidyapeeth (Deemed to be University)'s Institute of Management, Kolhapur, Maharashtra, India.
Volume - 15,
Issue - 9,
Year - 2022
In the present study, unani formulations Habb-e-Suranjan, Habb-e-Azaraqui are evaluated for the presence and quantification of phytochemicals, total antioxidant capacity and ferric reducing antioxidant capacity. The total phenolic and flavonoid content were estimated according to folin-ciocalteau and aluminum chloride colorimetric methods respectively. The total antioxidant capacity and ferric reducing antioxidant capacity were conducted according to standard methods. The results of phytochemical screening revealed the presence of phenols, flavonoids, saponins, phytosterols and tannins in Habb-e-Suranjan and Habb-e-Azaraqui. The quantitative study of total phenols and flavonoids in these formulations were expressed in terms of Gallic acid and Quercetin equivalents. The total antioxidant capacities of the formulations were less compared to standard ascorbic acid. Hence concluded that the studied unani formulations shown to possess phyto-therapeutic constituents which may be responsible for antioxidant and reducing power activities. Overall, the formulations are proving the claimed therapeutic activities by antioxidant mechanism.
Cite this article:
K. Ashok Kumar, Firoj A. Tamboli, Harinath N. More, Kamal M. Alaskar, Prashant G. Tandale. Phytochemical Screening, Total Flavonoid, Phenolic content assays and Antioxidant activity of selected Unani Formulations. Research Journal of Pharmacy and Technology. 2022; 15(9):4111-4. doi: 10.52711/0974-360X.2022.00690
K. Ashok Kumar, Firoj A. Tamboli, Harinath N. More, Kamal M. Alaskar, Prashant G. Tandale. Phytochemical Screening, Total Flavonoid, Phenolic content assays and Antioxidant activity of selected Unani Formulations. Research Journal of Pharmacy and Technology. 2022; 15(9):4111-4. doi: 10.52711/0974-360X.2022.00690 Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-9-50
1. B Ravishankar and VJ Shukla, Indian Systems of Medicine: A Brief Profile. Afr J Tradit Complement Altern Med. 2007; 4(3): 319–337.
2. Prasad LV. In: Indian System of Medicine and Homoeopathy Traditional Medicine in Asia. Chaudhury Ranjit Roy, Rafei Uton Muchatar., editors. New Delhi: WHO- Regional Office for South East Asia; 2002; 283–286
3. Shabnam Ansari, Qamar Alam Khan, Dr. Rubi Anjum, Aisha Siddiqui and Kamar Sultana, Fundamentals of unani system of medicine - a review. ejbps, 2017; Volume 4, Issue 9, 219-223.
4. AYUSH. Background. 2016. Available from: http://ayush.gov.in/.
5. Losada-Barreiro, S.; Bravo-Díaz, C. Free radicals and polyphenols: The redox chemistry of neurodegenerative diseases. Eur. J. Med. Chem. 2017; 133: 379–402. [CrossRef]
6. Madamanchi, N.R.; Vendrov, A.; Runge, M.S. Oxidative stress and vascular disease. Arter. Thromb. Vasc. Biol. 2005; 25: 29–38. [CrossRef]
7. Methodological considerations for characterizing potential antioxidant actions of bioactive components in plant foods. Aruoma OI Mutat Res. 2003; 523-524
8. Mohammed AA, Ibrahim AA. Pathological roles of reactive oxygen species and their defence mechanism. Saudi Pharm J. 2004;12:1–18.
9. Hakeem Mohammad Kabeeruddin, Bayaze Kabeer. 5th ed. Hyderabad: Hikmat Book Depot, 1938; 2: 96, 101, 57, 47, 16, 21, 20, 19, 127, 92, 126, 132, 129, 132, 136, 65, 71, 11, 93, 92.
10. Anonimus, Qarabadeen-e-Majeedi, Delhi printing works new delhi, 127, 101, 104, 47, 107, 43, 46, 59, 32, 55, 54, 51, 118, 319, 345, 346, 384, 346, 347, 355, 265, 346, 410, 262, 154, 156, 227, 213, 36.
11. Ibn Baitar. Aljame ul Mufradat al Advia wal aghzia. Urdu translation by CCRUM New Delhi. Department of AYUSH. Ministry of H&FW Govt. Of India, 2000; 2th: 170-173
12. V. Lobo, A. Patil, A. Phatak, and N. Chandra. Free radicals, antioxidants and functional foods: Impact on human health, Pharmacogn Rev. 2010; 4(8): 118–126.
13. Nabi MG. New Delhi: CCRUM, Ministry of H and F.W. Govt. of India; 2007; Makhzane Mufradat wa Murakkabat; p. 310.
14. Anonymous. National Formulary of Unani Medicine. Part 5. CCRUM: Ministry of health and family welfare, Govt. of India, Ramko Press Pvt. Ltd, New Delhi – 110 020, 2008; 36,15,67,72, 73, 81, 86, 87, 88, 89, 90, 92, 109, 27, 127, 141
15. Aneesh T. P., Mohammed Hisham, Sonal Sekhar M., Manjusree Madhu, Deepa T. V. International market scenario of traditional Indian herbal drugs – India declinin International Journal of Green Pharmacy 2009; July-September 184-190.
16. Parul Agarwal, Shashi Alok, Amreen Fatima and Amita Verma Current Scenario Of Herbal Technology World Wide: An Overview International Journal of Pharmaceutical Sciences and Research. 2013; 4(11): 4105-4117.
17. Alok Sharma, C. Shanker, Lalit Kumar Tyagi, Mahendra Singh and Ch.V.Rao Herbal Medicine for Market Potential in India: An Overview. Academic Journal of Plant Sciences 2008; 1 (2): 26-36.
18. Yamada, T.; Hayasaka, S.; Shibata, Y.; Ojima, T.; Saegusa, T.; Gotoh, T.; Ishikawa, S.; Nakamura, Y.; Kayaba, K. Frequency of citrus fruit intake is associated with the incidence of cardiovascular disease: The Jichi Medical School cohort study. J. Epidemiol. 2011; 21: 169–175.
19. Sri Hartati Yuliani, Dina Christin Ayuning Putri, Aris Widayati, Bagas Abiyoga. Compounding practice in a developing Country: A Case study of divided Powder in Indonesia. Research J. Pharm. and Tech. 2020; 13(12):6231-6237.
20. Khandelwal K. R., Sethi V. K., “Practical Pharmacognosy Techniques and Experiments”, Nirali Prakashan, Twenty- Fourth Edition, page no. 25.1-25.2, 2014.
21. V. Jenila Jose Jancy, V. Kalaichelvan, N. Balakrishnan. Phytochemical Analysis and Anti-Oxidant Activity of various Extracts of Plant Cassia auriculata. Research J. Pharm. and Tech. 2020; 13(12):6150-6155.
22. P. Chandrika, K. R. S. Prasad, Phani R S CH. Phytochemical screening and Evaluation of Anti-bacterial activity and various Anti-oxidant studies of root extracts of Mangrove plant Dalbergia spinosa. Research J. Pharm. and Tech 2020; 13(6):2551-2555.
23. Nitin Dubey, Nidhi Dubey, RS Mehta, AK Saluja, DK Jain. Preparation and Physico-chemical Characterization of Kushta-e-sadaf. A Traditional Unani Formulation. Research J. Pharm. and Tech. 1(3): July-Sept. 2008; Page 182-186.
24. Andressa Blainski, Gisely Cristiny Lopes and João Carlos Palazzo de Mello, Application and Analysis of the Folin Ciocalteu Method for the Determination of the Total Phenolic Content from Limonium Brasiliense L., Molecules 2013; 18: 6852-6865; doi:10.3390/molecules18066852
25. Firoj A. Tamboli, Harinath N. More, Evaluation of Anti ulcer and Antioxidant activity of Barleria gibsoni Dalz. leaves Pharmcognosy Research. 2016; 8(4): 226-230.
26. F. Pourmorad, S. J. Hosseinimehr, and N. Shahabimajd, “Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants,” African Journal of Biotechnology, 2006;(11): 1142–1145.
27. G. Miliauskas, P. R. Venskutonis, and T. A. van Beek, “Screening of radical scavenging activity of some medicinal and aromatic plant extracts.” Food Chemistry, 2004; vol. 85, no. 2, 231–237.
28. Abha Shukla, Amanpreet Kaur, Rishi Kumar Shukla, Anchal. Comparative Evaluation of Antioxidant capacity, Total flavonoid and Phenolic content of Ehretia acuminata R. Br. fruit. Research J. Pharm. and Tech. 2019; 12(4):1811-1816
29. Som K. Madhvi, Manik Sharma, Javaid Iqbal, Mohd Younis, Razda Sheikh. Phytochemical analysis, Total Flavonoid, Phenolic contents and Antioxidant activity of extracts from the Leaves of Rhododendron arboreum. Research J. Pharm. and Tech. 2020; 13(4):1701-1706.
30. P Prieto 1, M Pineda, M Aguilar, Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E, Anal Biochem,1999; May 1;269(2):337-41. doi: 10.1006/abio.1999.4019.
31. Benzie, Iris F. F.; Strain, J. J., The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay, Analytical Biochemistry 1996; 239 (1): 70-76CODEN: ANBCA2; ISSN:0003-2697. (Academic)
32. Sharmistha Banerjee, Shuchi Kaushik, Rajesh Singh Tomar. Efficacy Assay of Crude Leaf Extracts of Calotropis procera and Azadirachta indica for Anti-oxidant Activity. Research J. Pharm. and Tech 2018; 11(10): 4480-4486.
33. Spoorthy N. Babu, S. Govindarajan, M. A. Vijayalakshmi, Ayesha Noor. Evaluation of In vitro Anti-Diabetic and Anti-Oxidant activities and Preliminary Phytochemical screening of Gel, Epidermis and Flower extract of Aloe vera. Research J. Pharm. and Tech. 2019; 12(4):1761-1768.
34. S.B. Santhosh, M.J.N. Chandrasekar, Kaviarasan. L, P. Deepak, T. Silambarasan, B. Gayathri, D. Natarajan. Chemical Composition, Antibacterial, Anti-oxidant and Cytotoxic properties of Green Synthesized Silver Nanoparticles from Annona muricata L. (Annonaceae).Research J. Pharm. and Tech. 2020; 13(1):33-39.
35. Salemi Said, Gherraf Noureddine, Laouini Salah Eddine, Guerram Abdelmadjid, Berrani Djamel, Ali Tliba. Phenolic Content, HPLC Analysis and Antioxidant Activity Extract from Tamarix gallica and Tamarix articulata Growing in Southeast of Algeria. Research J. Pharm. and Tech 2018; 11(9): 3826-3832.