Author(s): Muhammad I. Aulia, Rita K. Sari, Deded S. Nawawi, Rizna T. Dewi

Email(s): rita_kartikasari@apps.ipb.ac.id

DOI: 10.52711/0974-360X.2022.00645   

Address: Muhammad I. Aulia1, Rita K. Sari1*, Deded S. Nawawi1, Rizna T. Dewi2
1Departement of Forest Products, Faculty of Forestry and Environment, Bogor Agricultural University, Dramaga, 16680, West Java, Indonesia.
2Indonesian Institute of Sciences Chemical Research Center, Indonesian Institute of Sciences (P2K-LIPI). Gd. 454 PUSPIPTEK, Serpong, 15314, South Tangerang, Banten, Indonesia.
*Corresponding Author

Published In:   Volume - 15,      Issue - 9,     Year - 2022


ABSTRACT:
Tropical forests in Indonesia have a great diversity of plants. Among those species, Acacia is a plant that is widely found in Indonesia. The utilization of Acacia in Indonesia increases the amount of bark waste in the harvesting process. The aim of the research was to obtain prospective extracts the bark of A. mangium, A. auriculiformis, and A. crassicarpa based on antioxidant activity, a-glucosidase inhibition assay, and to identify compound in the prospective extract. In this research, the methods used to determine antioxidant were used 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), antioxidant Ferric Reducing power (FRAP), and assays for the total levels of phenolic compounds and flavonoids in each extract. To determine antidiabetic, a test was carried out by examining the a-glucosidase inhibition of the a-glucosidase enzyme from Sachamyce cerevisiae. Then the prospective extracts were identified using Liquid Chromatography-Mass Spectrometry (LC-MS) and Nuclear Magnetic Resonance (NMR) (1H and 13C). Based on the test results, Acacia mangium methanol extract showed the best activity for testing. Separation and further purification of A. mangium extract obtained sub-fraction 6 (isolate 1) which was identified as scopoletin and shows the a-glucosidase inhibition at 96,84%. Based on the results of this research, A. mangium has the potential as a source of medicinal ingredients, and it is suggested to conduct further research to isolate other active compounds.


Cite this article:
Muhammad I. Aulia, Rita K. Sari, Deded S. Nawawi, Rizna T. Dewi. Isolation and Identification of Antioxidant and α-glucosidase Inhibitor Compound from Prospective Extract of Acacia Bark from Indonesia. Research Journal of Pharmacy and Technology. 2022; 15(9):3847-3. doi: 10.52711/0974-360X.2022.00645

Cite(Electronic):
Muhammad I. Aulia, Rita K. Sari, Deded S. Nawawi, Rizna T. Dewi. Isolation and Identification of Antioxidant and α-glucosidase Inhibitor Compound from Prospective Extract of Acacia Bark from Indonesia. Research Journal of Pharmacy and Technology. 2022; 15(9):3847-3. doi: 10.52711/0974-360X.2022.00645   Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-9-5


REFERENCES:
1.    Quraishi A. Continuous Culture of Acacia mangium Willd. Research J Science and Tech. 2012 Jul;4(4):168-171.
2.    Hendrati RL, Nurrohmah SH, Susilawati S, Budi S. Budidaya Acacia Uriculiformis (Acacia auriculiformis) untuk Kayu Energi. Bogor. 2014.
3.    Doran JC, Turnbull JW. Australian Trees and Shrubs: Species for Land Rehabilitation and Farm Planting in the Tropics. Canberra. 1997.
4.    Badan Pusat Statistik. 2019. Satistik Produksi Kehutanan 2019. Jakarta (ID): Badan Pusat Statistik.
5.    Purwanto D. Analisa jenis limbah kayu pada industri pengolahan kayu di Kalimantan Selatan. Jurnal Riset Industri Hasil Hutan. 2009 Jun; 1(1):14-20. doi:10.24111/jrihh.v1i1.864.
6.    Prayogo YH, Syafii W, Sari RK, Batubara I, Danu. Pharmacological activity and phytochemical profile of Acacia heartwood extracts. Scientia Pharmaceutica. 2021 Aug 4;89(3):1-11. doi:10.3390/scipharm89030037.
7.    Setyaningrum ED, Kartika R, Simanjuntak Partomuan. Uji skrining fitokimia dan uji aktivitas antioksidan dari ekstrak daun akasia (Acacia auriculiformis Benth.). Public knowledge Project. 2017 Dec 29;94-96.
8.    Pietarinen SV, Willfor SM, Ahotupa MO, Hemming JE, Holmbom BR. Knotwood and bark extracts: strong antioxidants from waste materials. Journal of Wood Science. 2006 Oct 1;52:436-444. doi:10.1007/s10086-005-0780-1.
9.    Chen X, Xiong J, He Q, Wang F. Characterization and potential antidiabetic activity of proanthocyanidins from the barks of Acacia mangium and Larix gmelinii. Journal of Chemistry. 2019 Mar 3;2019(5):1-9. doi:10.1155/2019/4793047.
10.    Adetutu A, Olorunnisola OS, Owoade OB. Nutritive values and antioxidant activity of citrullus lanatus fruit extract. Food and Nutrition Sciences. 2015 Aug 28;6(11):1056-1064. doi:10.4236/fns.2015.611109.
11.    Artanti N, Dewi RT, Maryani F. Pengaruh lokasi dan pelarut pengekstraksi terhadap kandungan fitokimia dan aktivitas antioksidan ekstrak pegagan (Centella asiatica L. Urb). Jurnal Kimia Terapan Indonesia. 2014 Dec;16(2):88-92. doi:10.14203/jkti.v16i2.13.
12.    Dudonne S, Vitrac X, Coutiere P, Woillez M, Merillon JM. Comparative study of antioxidant properties and total phenolic content of 30 plant extracts of industrial interest using DPPH , ABTS , FRAP , SOD , and ORAC assays. Journal Agricultural Food Chemistry. 2009 Feb 6;57(5):1768–1774. doi:10.1021/jf803011r.
13.    Lee KJ, Oh YC, Cho WK, Ma JY. Antioxidant and anti-inflamatory activity determination of one hundred kinds of pure chemical compounds using offline and online screening HPLC assay. Evidence-based Complementary Alternative Medicine. 2015 Jul 30;2015(2):1-13. doi:10.1155/2015/165457.
14.    Dewi RT, Tachibana S, Darmawan A. Effect on α-glucosidase inhibition and antioxidant activities of butyrolactone derivatives from Aspergillus terreus MC751. Medicinal Chemistry Research. 2014 Jan;23:454-460. doi:10.1007/s00044-013-0659-4.
15.    Sowndhararajan K, Joseph JM, Manian S. Antioxidant and free radical scavenging activities of Indian Acacias: Acacia leucophloea (Roxb.) Willd., Acacia ferruginea Dc., Acacia dealbata Link. and Acacia pennata (L.) Willd. International Journal of Food Properties. 2013 Jun 14;16(8):1717-1728. doi:10.1080/10942912.2011.604895.
16.    Guleria A, Tiku AK, singh G, Vyas D, Bhardwaj A. Antioxidant activity and protective effect against plasmid dna strand scission of leaf, bark, and heartwood extracts from Acacia catechu. Journal of Food Science. 2011 Aug;76(7):C959-964.
17.    Lukmandaru G. Extractive composition of mangium wood (acacia mangium). Jurnal Ilmu dan Teknologi Kayu Tropis. 2012 Jul;10(2):150-158. doi:10.51850/jitkt.v10i2.114.g110.
18.    Mutiar S, Kasim A, Emriadi, Asben A. Studi awal tanin dari kulit kayu Acacia auriculiformis A. Cunn. ex Benth. dari hutan tanaman industri untuk bahan penyamak kulit. Majalah Kulit, Karet, dan Plastik. 2018 Oct 22;34(2):41-48.
19.    Sayuti M. Pengaruh perbedaan metode ekstraksi, bagian dan jenis pelarut terhadap rendemen dan aktifitas antioksidan bambu laut (Isis Hippuris). Technology Science and Engineering Journal. 2017 Nov 3;1(3):166-174.
20.    Sathya A, Siddhuraju P. Role of phenolics as antioxidants, biomolecule protectors and as anti-diabetic factors - Evaluation on bark and empty pods of Acacia auriculiformis. Asian Pacific Journal of Tropical Medicine 2012 Oct 20;5(10):757-765.
21.    Mokrani A, Madani K. Effect of solvent, time and temperature on the extraction of phenolic compounds and antioxidant capacity of peach (Prunus persica L.) fruit. Separation and Purification Technology. 2016 Apr 13;162:68-76.
22.    Uma DB, Ho CW, Wan Aida WM. Optimization of extraction parameters of total phenolic compounds from henna (Lawsonia inermis) leaves. Sains Malaysiana. 2010 Dec 30;39(1):119-128.
23.    Rifai G, Widarta WR, Nocianitri KA. Pengaruh jenis pelarut dan rasio bahan dengan pelarut terhadap kandungan senyawa fenolik dan aktivitas antioksidan ekstrak biji alpukat (Persea americana Mill.). Jurnal Ilmu dan Teknologi Pangan. 2018 Jul 29;7(2):22-32. doi:10.24843/itepa.2018.v07.i02.p03.
24.    Azizah DN, Kumolowati E, Faramatuda F. Penetapan kadar flavonoid metode alcl3 pada ekstrak metanol kulit buah kakao (Theobroma cacao L.). Kartika Jurnal Ilmiah Farmasi. 2014 Dec;2(2):45-49. doi:10.26874/kjif.v2i2.14.
25.    Pękal A, Pyrzynska K. Evaluation of Aluminium complexation reaction for flavonoid content assay. Food Analytical Methods. 2014 Feb 11;7(9):1776-1782. doi:10.1007/s12161-014-9814-x.
26.    Jun M, Fu H Y, Hong J, Wan X, Yang CS, Ho C-T. Comparison of antioxidant activities of isoflavones from kudzu root (Pueraria lobate ohwi). Journal Food of Science. 2006 Jul 20;68(6):2117-2122. doi:10.1111/j.1365-2621.2003.tb07029.x.
27.    Shukla A, Kaur A, Shukla RK, Anchal. Comparative Evaluation of Antioxidant capacity, Total flavonoid andPhenolic content of Ehretia acuminata R. Br. Fruit. Research J Pharm and Tech.2019 Apr;12(4):1811-1816.
28.    Pethani T, Thumar M, Vaishnav D, Dudhrejiya A. A Concise Study of Pharmacognostic and Antioxidant Potential of Stem bark from Bauhinia variegata Linn. Research J Pharm and Tech. 2018 Aug;11(8):3250-3256.
29.    Jainn, Goyal S, Ramawat KG. Radical Scavenging Activity and Total Phenolic Content in Selected Fruits and Vegetables. Research J Pharm and Tech. 2012 Jan;5(1):121-124.
30.    Tapas A, Sakarkar D, Kakde R. The Chemistry and Biology of Bioflavonoids. Research J Pharm and Tech. 2008 July-Sept;1(3);132-143.  
31.    Chandrawanshi NK, Tandia DK, Jadhav SK. Determination of Antioxidant and Antidiabetic Activities of Polar Solvent Extracts of Daedaleopsis confragosa (Bolton) J. Schröt. Research J Pharm and Tech. 2018 December;11(12):5623-5630.
32.    Ganamé HT, Karanga Y, Ilboudo O, Nikiema WKHC, Sawadogo RW, Tapsoba I. α-Glucosidase inhibitory and antiradical properties of Acacia macrostachya. European Journal of Medical and Health Sciences. 2020 Sept 18;2(5):1-6.
33.    Babu SN, Govindarajan S, Vijayalakshmi MA, Noor A. Evaluation of In vitro Anti-Diabetic and Anti-Oxidant activities and Preliminary Phytochemical screening of Gel, Epidermis and Flower extract of Aloe vera. Research J Pharm and Tech. 2019 Apr;12(4):1761-1768.
34.    Bhutkar MA, Bhise SB. Comparative Studies on Antioxidant Activity of Some Antidiabetic Plants. Research J Pharm and Tech. 2011 Sept;4(9):1209-1412.
35.    Anisah LN, Syafii W, Pari G, Sari RK. Antidiabetic activities and identification of chemical compound from Samama (Anthocephalus macrophyllus (Roxb) Havil). Indonesian Journal Chemistry. 2018 Aug 31:18(1):66-74. doi:10.22146/ijc.25492.
36.    Isma C, Aminah NS, Kristanti AN. Skopoletin suatu senyawa fenilpropanoid dari ekstrak etik asetat umbi jalar (Ipomoea batatas L.). Jurnal Kimia Riset. 2018 Dec 20;3(2):116-121. doi:10.20473/jkr.v3i2.12061.
37.    Rao USM, Mohd KS, Atif AB. Scopoletin (Coumarin extracted from Mengkudu): Chemical, Biological, Molecular and Pharmacokinetic insights – A Review. Research J Pharm and Tech. 2013 Sept;6(9):978-984.
38.    Khamis M, Talib F, Rosli NS, Dharmaraj S, Mohd S, Srenivasan S, Latif ZA, Utharkar MRS. In vitro α-amylase and α-glucosidase inhibition and increased glucose uptake of Morinda citrifolia fruit and scopoletin. Research J Pharm and Tech. 2015 February;8(2):189-193.

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags


Not Available